Immobilization of HRP enzyme on polymeric microspheres and its use in decolourisation of organic dyes.

In this study, horseradish peroxidase (HRP) enzyme was immobilized on Pd(II) containing polymeric microspheres by adsorption method and used for the decolourisation of Methyl Orange (MO) and Rhodamine B (RB) dyes. The synthesized microspheres were characterized by Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy-Energy Dispersive X-ray (SEM/EDX), Thermal Gravimetric Analysis (TGA). The effects of pH, dye concentration, temperature, and H2O2 concentration on the decolourisation of MO and RB were determined. According to the results of various parameters studied, when 2-AEPS-napht-HRP support was used, MO and RB were biodegraded to 69.72% and 80.65%, respectively, within 60 min. When 2-AEPS-napht-Pd-HRP support was used, MO and RB were biodegraded to 58.35% and 90.81%, respectively, under optimum conditions. When the reproducibility results of the immobilized supports were examined, it was observed that they remained efficient during the first five reusability cycles and even reached 65% decolourisation efficiency after the 9th reuse. The immobilized enzyme (2AEPS-npht-HRP and 2AEPS-npht-Pd-HRP) showed remarkable resistance to higher temperatures compared to the free enzyme.

Harnessing iron materials for enhanced decolorization of azo dye wastewater: A comprehensive review.

Highly colored azo dye-contaminated wastewater poses significant environmental threats and requires effective treatment before discharge. The anaerobic azo dye treatment method is a cost-effective and environmentally friendly solution, while its time-consuming and inefficient processes present substantial challenges for industrial scaling. Thus, the use of iron materials presents a promising alternative. Laboratory studies have demonstrated that systems coupled with iron materials enhance the decolorization efficiency and reduce the processing time. To fully realize the potential of iron materials for anaerobic azo dye treatment, a comprehensive synthesis and evaluation based on individual-related research studies, which have not been conducted to date, are necessary. This review provides, for the first time, an extensive and detailed overview of the utilization of iron materials for azo dye treatment, with a focus on decolorization. It assesses the treatment potential, analyzes the influencing factors and their impacts, and proposes metabolic pathways to enhance anaerobic dye treatment using iron materials. The physicochemical characteristics of iron materials are also discussed to elucidate the mechanisms behind the enhanced bioreduction of azo dyes. This study further addresses the current obstacles and outlines future prospects for industrial-scale application of iron-coupled treatment systems.

Decolorization and degradation of crystal violet dye by electron beam radiation: Performance, degradation pathways, and synergetic effect with peroxymonosulfate.

Ionizing radiation (mainly including gamma ray and electron beam) technology provides a more efficient and ecological option for dye-containing wastewater treatment, which is supported by its successful achievements in industrial-scale applications. However, the degradation pathway of triphenylmethane dyes by radiation technology is still unclear. In this study, crystal violet (CV) was selected as representative cationic triphenylmethane dye, the decolorization and degradation performance by electron beam radiation technology was systematically evaluated. The results showed that CV can be efficiently decolorized and mineralized by radiation, and its degradation kinetics followed the first-order kinetic model. The effect of inorganic anions and chelating agents commonly existed in dye-containing wastewater on CV decolorization and total organic carbon (TOC) removal was explored. Quenching experiments, density functional theory (DFT) calculation and high performance liquid chromatography mass spectrometry (HPLC-MS) analysis were employed to reveal CV decolorization and degradation mechanism and pathway, which mainly included N-demethylation, triphenylmethane chromophore cleavage, ring-opening of aromatic products and further oxidation to carboxylic acid, and mineralization to CO2 and H2O. Additionally, electron beam radiation/PMS process was explored to decrease the absorbed dose required for decolorization and degradation, and the synergetic effect of radiation with PMS was elucidated. More importantly, the findings of this study would provide the support for treating actual dyeing wastewater by electron beam radiation technology.

A hybrid electrocoagulation-biocomposite adsorption system for the decolourization of dye wastewater.

Among the various methods for the removal of azo dye, electrocoagulation is recognized to be highly efficient. However, the process is associated with high operation and maintenance cost, which demands the need for reducing the electrolysis time without compromising the performance efficiency. This can be achieved by adopting hybrid electrocoagulation process with a low-cost but effective process, such as adsorption. The study investigated the performance of a hybrid electrocoagulation-biocomposite system (H-EC-BC) for removing methyl orange dye. Firstly, the operating parameters of electrocoagulation process were optimized and a removal efficiency of 99% has been attained using Fe-SS electrodes at a pH of 6 for a reaction time of 30 min. The performance of EC process was found to be decreasing with increase in dye concentration. Secondly, biocomposite was synthesized from Psidium guajava leaves and characterized using SEM, FTIR, EDAX, and XRD analyses. The results suggested that it is having a porous nature and cellulose crystal structure and confirmed the presence of chemical elements such as carbon (65.2%), oxygen (29.1%) as primary with Fe, Cl, Na and Ca as secondary elements. The performance of the biocomposite was evaluated for the dye adsorption using spectrophotometric methods. Various operating parameters were optimized using experimental methods and a maximum removal efficiency of 65% was achieved at a pH of 6, dosage of 5 g/L and an adsorption contact time of 120 min. The maximum efficiency (92.78%) was obtained with Fe-SS electrodes and KCl as a sustaining electrolyte under acidic circumstances (pH 6). The biocomposite was observed to be more efficient for higher dye concentration. Langmuir and Freundlich adsorption isotherms were fitted with the experimental results with R2 values as 0.926 and 0.980 respectively. The adsorption kinetics were described using Pseudo-first and Pseudo-second order models, wherein Pseudo-second order model fits the experimental results with R2 value of 0.999. The energy consumption of electrocoagulation (EC) process in the hybrid H-EC-BC system was compared to that of a standard EC process. The results demonstrated that the hybrid system is approximately 7 times more energy efficient than the conventional process, thereby implicating its adaptability for field application.

New insights on the decolorization of waste flows by Saccharomyces cerevisiae strain - A systematic review.

One of the common causes of water pollution is the presence of toxic dye-based effluents, which can pose a serious threat to the ecosystem and human health. The application of Saccharomyces cerevisiae (S. cerevisiae) for wastewater decolorization has been widely investigated due to their efficient removal and eco-friendly treatments. This review attempts to create an awareness of different forms and methods of using Saccharomyces cerevisiae (S. cerevisiae) for wastewater decolorization through a systematic approach. Overall, some suggestions on classification of dyes and related environmental/health problems, and treatment methods are discussed. Besides, the mechanisms of dye removal by S. cerevisiae including biosorption, bioaccumulation, and biodegradation and cell immobilization methods such as adsorption, covalent binding, encapsulation, entrapment, and self-aggregation are discussed. This review would help to inspire the exploration of more creative methods for applications and modification of S. cerevisiae and its further practical applications.

Regenerated Silk Nanofibers for Robust and Cyclic Adsorption-Desorption on Anionic Dyes.

In recent years, adsorption-based membranes have been widely investigated to remove and separate textile pollutants. However, cyclic adsorption-desorption to reuse a single adsorbent and clear scientific evidence for the adsorption-desorption mechanism remains challenging. Herein, silk nanofibers were used to assess the adsorption potential for the typical anionic dyes from an aqueous medium, and they show great potential toward the removal of acid dyes from the aqueous solution with an adsorption rate of ∼98% in a 1 min interaction. Further, we measured the filtration proficiency of a silk nanofiber membrane in order to propose a continuous mechanism for the removal of acid blue dye, and a complete rejection was observed with a maximum permeability rate of ∼360 ± 5 L·m-2·h-1. The Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy studies demonstrate that this fast adsorption occurs due to multiple interactions between the dye molecule and the adsorbent substrate. The as-prepared material also shows remarkable results in desorption. A 50-time cycle exhibits complete adsorption and desorption ability, which not only facilitates high removal aptitude but also produces less solid waste than other conventional adsorbents. Additionally, fluorescent 2-bromo-2-methyl-propionic acid (abbreviated as EtOxPY)-silk nanofibers can facilitate to illustrate a clear adsorption and desorption mechanism. Therefore, the above-prescribed results make electrospun silk nanofibers a suitable choice for removing anionic dyes in real-time applications.

Factors Influencing Decolourization and Detoxification of Remazol Brilliant Blue R Dye by Aspergillus flavus.

<b>Background and Objectives:</b> Anthraquinone synthetic dyes are widely used in textile, dyeing and paper painting. The discharge of these dyes into the environment causes detriment. The removal of physiochemical dyes is sometimes unsuccessful and expensive. Biological removal is inexpensive, eco-friendly and may break down organic contaminants. In the current work, a fungal technique was applied to decolorize and detoxify dye. <b>Materials and Methods:</b> Dye decolorizing fungi isolation, selection and identification of the most effective isolate and dye decolorization optimization based on carbon and nitrogen sources. In addition, the product's cytotoxicity and metabolites were tested. The enzyme activities were measured to determine dye decolorization. <b>Results:</b> Decolorization of reactive blue 19 dye by the most effective fungal strain isolate (5BF) isolated from industrial effluents were studied. This isolate was identified as <i>Aspergillus flavus</i> based on phenotypic characteristics and confirmed using 18S rRNA gene sequencing. Thin-layer chromatography indicated that this strain is aflatoxins free. Furthermore, metabolites produced from dye treatment with <i>A. flavus</i> were assessed using gas chromatography-mass spectrometry. Toxicity data revealed that <i>A. flavus</i> metabolites were not toxic to plants. Using a one-factor-at-a-time optimization technique, a maximum decolorization percentage (99%) was obtained after 72 hrs in the presence of mannitol and NH₄NO₃ or NH₄Cl as carbon and nitrogen sources. Two enzymes (laccase and manganese peroxidase) were shown to be active during dye decolorization by <i>A. flavus</i>. <b>Conclusion:</b> The <i>A. flavus</i> strain was shown to be safe when it came to removing dye from a synthetic medium with high efficiency and their metabolites had no negative influence on the environment. As a result, this strain will be used in the future for dye wastewater bioremediation.

A screening method for decoloration of xenobiotic dyes by fungi.

Wood degrading fungi are often screened for their ability to degrade xenobiotics such as dyes. Dye decoloration by these fungi on solid media could until now only be assessed qualitatively. We here describe a fast quantitative method to screen for dye decoloration on such media. Decoloration of crystal violet (CV), malachite green (MG), orange G (OG), rose bengal (RB) and remazol brilliant blue R (RBBR) by 124 isolates of the basidiomycete Schizophyllum commune was quantified with a flatbed scanner and the CIE-L*a*b* model. Colour and intensity changes were calculated with the Euclidean distance formula. More than 10 strains showed high MG decoloration. Isolates 136, 140 and 213 showed superior CV decoloration, while OG was most effectively decolorized by isolates 183, 216 and 227. Six strains showed high RB decoloration with isolate 216 being superior. The latter strain was also highly active on RBBR together with isolates 177 and 227. Together, dye decoloration was highly variable between the 124 isolates but strain 216 showed high activity on 3 out of 5 dyes. The fast screening method described in this paper enables identification of strains effectively decolorizing dyes.

Dye-decolorization of a newly isolated strain Bacillus amyloliquefaciens W36.

Dye-decolorization is one of the most important steps in dye-polluted wastewater treatment. The dye-decolorization bacteria were isolated from active sludge collected from wastewater treating pond of a dyeing and printing plant using serial dilution method. Among the 44 bacteria isolates from the active sludge, the strain Bacillus amyloliquefaciens W36 was found to have strong ability in dye-decolorization. The effects of carbon source, nitrogen sources, C/N, metal ions, temperature, pH, and rotation speed for dye-decolorization were investigated. The optimum decolorization conditions were that the strain was grown in enriched mineral salt medium (EMSM) using maltose 1 g/L, (NH4)2SO4 1 g/L as carbon and nitrogen source respectively, supplemented with 100 mg/L different dyes (pH 6.0), at 30 °C, 200 rpm from 48 to 96 h. The bacteria could aerobically decolorize dyes, such as Coomassie brilliant blue (95.42%), Bromcresol purple (93.34%), Congo red (72.37%) and Sarranine (61.7%), within 96 h. The dyes decolorization products were analyzed by ultra-violet and visible (UV-vis) spectroscopy before and after decolorization, which indicated that the four dyes were significantly degraded by the strain. The results indicated that the bacteria Bacillus amyloliquefaciens W36 could be used in dye-polluted wastewater treatment.

Understanding molecular enzymology of porphyrin-binding α + ß barrel proteins - One fold, multiple functions.

There is a high functional diversity within the structural superfamily of porphyrin-binding dimeric α + ß barrel proteins. In this review we aim to analyze structural constraints of chlorite dismutases, dye-decolorizing peroxidases and coproheme decarboxylases in detail. We identify regions of structural variations within the highly conserved fold, which are most likely crucial for functional specificities. The loop linking the two ferredoxin-like domains within one subunit can be of different sequence lengths and can adopt various structural conformations, consequently defining the shape of the substrate channels and the respective active site architectures. The redox cofactor, heme b or coproheme, is oriented differently in either of the analyzed enzymes. By thoroughly dissecting available structures and discussing all available results in the context of the respective functional mechanisms of each of these redox-active enzymes, we highlight unsolved mechanistic questions in order to spark future research in this field.

Development of photochemical integrated submerged membrane bioreactor for textile dyeing wastewater treatment.

A pilot-scale photocatalytic membrane bioreactor (PMBR) was developed for the treatment of textile dyeing wastewater. The PMBR is made of mild steel rectangular reactor of photocatalytic unit and polyethersulphone submerged hollow fibre membrane bioreactor unit with the working volume of about 20 L. For easy recovery, the tungsten oxide (WO3) and WO3/1% graphene oxide (GO)-powdered photocatalyst were made into bead and immersed in photocatalytic reactor. Graphene oxide incorporation has shown better results in decolourisation and degradation when compared with WO3 alginate alone. The incorporation of GO into WO3 minimises the recombination of photogenerated electron-hole pairs. The operating conditions such as 3 h of contact time for photocatalysis reaction (WO3/1% GO), 10 h hydraulic retention time for MBR and 100 kPa of transmembrane pressure were optimised. Chemical oxygen demand removal efficiency of 48% was attained with photocatalysis, and the removal efficiency was further increased up to 76% when integrated with MBR. The colour removal efficiency after photocatalysis was 25% further increased up to 70% with MBR. Complete total suspended solid removal has been achieved with this hybrid system.

Optimization of spore laccase production by Bacillus amyloliquefaciens isolated from wastewater and its potential in green biodecolorization of synthetic textile dyes.

The spore laccase enzyme production by B. amyloliquefaciens was optimized. It was characterized and tested for its textile dye decolorization potential. LB medium was found to be the most promising growth medium with addition of glucose (1-2%), yeast extract (0.1%), FeCl3 (0.01 mM) and MnCl2 (0.001 mM). The optimum spore laccase production was at pH 8, 30 °C, 1:5 medium to air ratio, 2% inoculum size and 7 days incubation. The characterization study of the enzyme showed the maximum activity at 60 °C and pH 6-7.5. It was induced by Ca+2, Mg+2, Fe+3, Zn+2, Cu+2 and Na+ at 1 mM concentration. Also, it was stable in the presence of methanol, ethanol, acetone and chloroform. In addition, it enhanced about 34% by 5 mM H2O2 and it was nearly stable at 10-20 mM H2O2. Furthermore, mediators such as ABTS, syrengaldazine and 2, 6 dimethyl phenol enhanced the spore laccase activity. The spore laccase enzyme efficiently decolorized direct red 81 and acid black 24 after 24 h. Phytotoxicity of the direct red 81 solution after decolorization by tested spore laccase was lower than that of the untreated dye solution. Finally, this study added a promising spore laccase candidate for ecofriendly and cost-effective dye wastewater bio-decolorization.

Screening and identification of newly isolated Pseudomonas sp. for biodegrading the textile azo dye C.I. Procion Red H-3B.

AIM: To test the potential of a newly isolated strain of Pseudomonas sp., and its optimization for carrying out bioremediation of textile azo dye Procion Red H-3B. METHOD: The isolation of the bacterial strain was done from a textile waste dumping site, followed by screening techniques to study the decolourization of an azo dye. The isolated pure culture was selected by its ability to form clear zones. The biochemical tests gave partial confirmation of the isolates, and the phylogenic analysis made the complete confirmation by 16S rRNA sequencing. RESULT: The identified strain belongs to the genus Pseudomonas. The phylogenic analysis confirmed that the strain belongs to Pseudomonas stutzeri. The culture exhibited maximum decolourization at pH between 6 and 8, the optimum at pH 7·5 and 37°C temperature. A maximum of 96% discolouration was observed at 50 mg l-1 of initial dye concentration after 24 h of incubation period. At a dye concentration equally or greater than 600 mg l-1 , the colour removal was drastically decreased to 30%. The use of fructose at 1% (w/v) and peptone 0·5% (w/v) concentration for 24 h of incubation, as carbon and nitrogen source, showed luxuriant decolourization. The results showed that the Pseudomonas sp. holds immense potential in treating textile effluents containing the dye Procion red H-3B. CONCLUSION: Pseudomonas is a known organism in bioremediation of various textile dyes but not much has being reported about the role of P. stutzeri in the bioremediation of azo dyes. This study revealed the immense potential of this strain in degrading the azo dyes. SIGNIFICANCE AND IMPACT OF THE STUDY: The strain shows prospective for industrial application in the field of textile wastewater treatment. Bioremediation is comparatively cheaper and more effective treatment, thus holds promising future for a cleaner environment.

Industrial polluted soil borne fungi decolorize the recalcitrant azo dyes Synozol red HF-6BN and Synozol black B.

Today's world needs to control the industrial pollution through smarter ways. Presently, we observed the capacity of soil borne fungi to digest Synozol Red HF-6BN and Synozol Black B. Initially, 86 fungal strains were isolated from soil samples randomly collected from industrial sites. Among these, 31 isolates were capable of dye decolorization on solid media, with SN12f and SN13a isolates showed the highest decolorization capacity. The dye decolorization by both strains was higher (80-95%), when incubated for 120 h under optimized conditions of pH, concentration, nutrient source and temperature. The dye (Synozol red HF-6BN and Synozol black B) decolorization by SN12f isolate was maximum (˃90%) at pH7, whereas the SN13a decolorized 90% of Synozol red HF-6BN and 89% of Synozol black B at pH3. The SN13a and SN12f isolates at 40 mg/L showed de-colorization of 94.71%, 81.4% (for Synozol red HF-6BN) and 90.5%, 84.4% (Synozol black B), respectively. Our isolates also mitigated the toxic effect of azo dyes on the growth of phosphate solubilizing soil bacteria. In fact, the untreated effluent showed toxic effects on the growth of beneficial bacterial by developing zone of inhibition (16.5 mm around Aeromonas spp., 14.5 mm around Sallmonella while 14.25 mm around Citrobacter spp). However, the fungal treated dye was unable to develop zone of inhibition. Laccase activity was positive for both of fungal isolates after incubation on Bassnell Hass Medium (0.0733 U/mL for SN12f and 0.0439 U/mL SN13a). Using molecular approaches (ITS region), SN12f was identified as Aspergillus nidulans, while SN13a as Aspergillus fumigatus. The current study showed that local fungal flora can reclaim the contaminated soils and support the agro-friendly micro-flora.

Using a novel polysaccharide BM2 produced by Bacillus megaterium strain PL8 as an efficient bioflocculant for wastewater treatment.

In this study, the purification and characterization of a novel polysaccharide-based bioflocculant BM2 produced by a bacterium Bacillus megaterium strain PL8 with self-flocculating property were investigated. The results showed that BM2 was an acidic polysaccharide composed of Gal, GalUA, Glc, GlcUA and Man at a molar ratio of 45.1: 33.8:9.3:9.2:2.4, respectively. The molecular weight of BM2 was 4.55 × 106 Da. BM2 had high flocculation efficiencies across a wide pH ranged from 4 to 11 and a wide temperature ranged from 20 to 100 °C towards kaolin clay. BM2 was a cation-independent bioflocculant which could achieve high flocculation activity without the addition of other cations. Adsorption bridging was the main mechanism in the flocculation process of BM2 towards kaolin clay. The BM2 also displayed a high removal efficiency in terms of Congo red (88.14%) and Pb2+ ions (82.64%). These results suggested that BM2 had a great potential as an efficient bioflocculant candidate in wastewater treatment.

Elucidation of fungal dye-decolourizing peroxidase (DyP) and ligninolytic enzyme activities in decolourization and mineralization of azo dyes.

AIM: The aim of the study is to investigate the efficiency of Geotrichum candidum in the decolourization and mineralization of synthetic azo dyes. METHODS AND RESULTS: It includes screening of enzymes from G. candidum and its optimization, followed by decolourization and mineralization studies. Decolourization was observed to be maximum in methyl orange (94·6%) followed by Congo red (85%), trypan blue (70·4%) and Eriochrome Black T (55·6%) in 48 h, suggesting the plausible degradation of the azo dyes by G. candidum. The enzyme activity study showed that DyP-type peroxidase has highest activity of 900 mU ml-1 compared to that of laccase (405 mU ml-1 ) and lignin peroxidase (LiP) (324 mU ml-1 ) at optimized pH (6) and temperature (35°C). Moreover, the rate of decolourization was found to be directly proportional to the production of laccase and LiP, unlike DyP-type peroxidase. Furthermore, mineralization study demonstrated reduction in aromatic amines, showing 20% mineralization of methyl orange. CONCLUSION: Geotrichum candidum with its enzyme system is able to efficiently decolourize and mineralize the experimental azo dyes. SIGNIFICANCE AND IMPACT OF THE STUDY: The efficient decolourization and mineralization of azo dyes makes G. candidum a promising alternative in the treatment of textile effluent contaminated with azo dyes.

Chitosan-wrapped multiwalled carbon nanotube as filler within PEBA thin film nanocomposite (TFN) membrane to improve dye removal.

Herein, a thin film nanocomposite (TFN) membrane was prepared through deposition of a very thin mixed matrix layer of PEBAX®1657/chitosan-wrapped multiwalled carbon nanotubes (CWNTs) on an ultraporous polyethersulfone (PES) substrate. The eco-friendly CWNTs were synthesized via non-covalent functionalization of MWNTs by carbohydrate polymer chitosan. They were then incorporated into PEBAX®1657 matrix at different loadings (0, 0.1, 0.5, 1 and 2 wt%). The membranes were analyzed using SEM, AFM, FTIR, XRD and contact angel analyses. Furthermore, pure water fluxes through the membranes were investigated at 1, 2 and 3 bar and Malachite green separation properties of the membranes were evaluated at 2 bar. The results showed that the highest permeate flux (∼13.85 L/m2h) and rejection (∼98.7%) were obtained at 1 wt% and 0.1 wt% CWNT dosages, respectively. Additionally, the slight flux decline of the membranes during 5 h indicated the improved antifouling properties.

Biochar-activated persulfate for organic contaminants removal: Efficiency, mechanisms and influencing factors.

Turning biomass into biochar as a multifunctional carbon-based material for water remediation has attracted much research attention. Sawdust and rice husk were selected as feedstock for biochar (BC) production, aiming to explore their performance as a catalyst to activate persulfate (PS) for degrading acid orange 7 (AO7). There was an excellent synergistic effect in the combined BC/PS system. Sawdust biochar (MX) showed a faster and more efficient performance for the AO7 degradation due to its abundant oxygen functional groups, compared to rice husk biochar (DK). In the BC/PS system, AO7 was well decolorized and mineralized. Based on the two-dimensional correlation analysis method, the azo conjugation structure and naphthalene ring of AO7 molecule changed first then benzene ring changed during the reaction. Moreover, AO7 decolorization efficiency increased with the increase of PS concentration and biochar dosage, and the deacrease of pH. Biochar deactivated after used twice. When the biochar reached its adsorption equilibrium of AO7, the AO7 could not be degraded in the BC/PS system. SO4- and OH participated in the reaction together and OH played the main role in activating PS to AO7 decolorization based on the radical scavengers experiment. All of results indicate using biochar to activate PS for degradation of AO7 contaminated water is a promising method.

Electrochemical oxidation of indanthrene blue dye in a filter-press flow reactor and toxicity analyses with Raphidocelis subcapitata and Lactuca sativa.

Alternative routes to degrade dyes are of crucial importance for the environment. Hence, we report the electrochemical removal of indanthrene blue by using a boron-doped diamond anode, focusing on the toxicity of the treated solutions. Different operational conditions were studied, such as current density (5, 10, and 20 mA cm-2) and electrolyte composition (Na2SO4, Na2CO3, and NaNO3). Besides, the pH was monitored throughout the experiment to consider its direct influence on the ecotoxicity effects. The highest electrochemical oxidation efficiency, measured as color removal, was seen in the 180 min condition of electrolysis in 0.033 M Na2SO4, applying 20 mA cm-2, resulting in a color removal of nearly 91% and 40.51 kWh m-3 of energy consumption. The toxicity towards Lactuca sativa depends solely on pH variations being indifferent to color removal. While the inhibition concentration (IC50) for Raphidocelis subcapitata increases 20% after treatment (in optimized conditions), suggesting that the byproducts are more toxic for this specific organism. Our data highlight the importance of analyzing the toxicity towards various organisms to understand the toxic effect of the treatment applied.

Microbial Decolorization of Triazo Dye, Direct Blue 71: An Optimization Approach Using Response Surface Methodology (RSM) and Artificial Neural Network (ANN).

The release of wastewater from textile dyeing industrial sectors is a huge concern with regard to pollution as the treatment of these waters is truly a challenging process. Hence, this study investigates the triazo bond Direct Blue 71 (DB71) dye decolorization and degradation dye by a mixed bacterial culture in the deficiency source of carbon and nitrogen. The metagenomics analysis found that the microbial community consists of a major bacterial group of Acinetobacter (30%), Comamonas (11%), Aeromonadaceae (10%), Pseudomonas (10%), Flavobacterium (8%), Porphyromonadaceae (6%), and Enterobacteriaceae (4%). The richest phylum includes Proteobacteria (78.61%), followed by Bacteroidetes (14.48%) and Firmicutes (3.08%). The decolorization process optimization was effectively done by using response surface methodology (RSM) and artificial neural network (ANN). The experimental variables of dye concentration, yeast extract, and pH show a significant effect on DB71 dye decolorization percentage. Over a comparative scale, the ANN model has higher prediction and accuracy in the fitness compared to the RSM model proven by approximated R 2 and AAD values. The results acquired signify an efficient decolorization of DB71 dye by a mixed bacterial culture.