RESUMO
Recent in vivo tracer studies demonstrated that targeted mass spectrometry (MS) on the Q Exactive Orbitrap could determine the metabolism of HDL proteins 100s-fold less abundant than apolipoprotein A1 (APOA1). In this study, we demonstrate that the Orbitrap Lumos can measure tracer in proteins whose abundances are 1000s-fold less than APOA1, specifically the lipid transfer proteins phospholipid transfer protein (PLTP), cholesterol ester transfer protein (CETP), and lecithin-cholesterol acyl transferase (LCAT). Relative to the Q Exactive, the Lumos improved tracer detection by reducing tracer enrichment compression, thereby providing consistent enrichment data across multiple HDL sizes from 6 participants. We determined by compartmental modeling that PLTP is secreted in medium and large HDL (alpha2, alpha1, and alpha0) and is transferred from medium to larger sizes during circulation from where it is catabolized. CETP is secreted mainly in alpha1 and alpha2 and remains in these sizes during circulation. LCAT is secreted mainly in medium and small HDL (alpha2, alpha3, prebeta). Unlike PLTP and CETP, LCAT's appearance on HDL is markedly delayed, indicating that LCAT may reside for a time outside of systemic circulation before attaching to HDL in plasma. The determination of these lipid transfer proteins' unique metabolic structures was possible due to advances in MS technologies.
Assuntos
Proteínas de Transferência de Ésteres de Colesterol/sangue , Lipoproteínas HDL/sangue , Espectrometria de Massas/métodos , Fosfatidilcolina-Esterol O-Aciltransferase/sangue , Proteínas de Transferência de Fosfolipídeos/sangue , Análise Química do Sangue/instrumentação , Análise Química do Sangue/métodos , Deutério/análise , Deutério/sangue , Feminino , Humanos , Cinética , Lipoproteínas HDL/química , Masculino , Espectrometria de Massas/instrumentação , Modelos Biológicos , Peso Molecular , Tamanho da PartículaRESUMO
The effects of deuterium depletion on the human organism have been, except for the antitumor action, seldom investigated by now and the available data are scarce. In oncological patients who also suffered from diabetes and were treated with deuterium-depleted water (DDW), an improvement of glucose metabolism was observed, and rat studies also proved the efficacy of DDW to reduce blood sugar level. In the present work, 30 volunteers with pre- or manifest diabetes were enrolled to a clinical study. The patients received 1.5 L of water with reduced deuterium content (104 ppm instead of 145 ppm, equivalent 12 mmol/L in human) daily for 90 days. The effects on fasting glucose and insulin level, on peripheral glucose disposal, and other metabolic parameters were investigated. Fasting insulin and glucose decreased, and insulin reaction on glucose load improved, in 15 subjects, while in the other 15 the changes were opposite. Peripheral glucose disposal was improved in 11 of the subjects. In the majority of the subjects, substantial increase of serum high-density lipoprotein (HDL) cholesterol and significant decrease of serum Na+ concentration were also seen-the latter possibly due to activation of a Na+/H+ antiporter by the decreased intracellular deuterium level. The results support the possible beneficial role of DDW in disorders of glucose metabolism but leave questions open, requiring further studies.
Assuntos
Deutério/sangue , Jejum/sangue , Síndrome Metabólica/sangue , Adulto , Glicemia/metabolismo , Feminino , Humanos , Insulina/metabolismo , Masculino , Pessoa de Meia-Idade , Água/administração & dosagemRESUMO
A candidate reference measurement procedure (RMP) based on isotope dilution coupled with liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) was developed and validated for the quantification of 17α-hydroxyprogesterone (17-OHP) in human plasma. 17-OHP spiked with a deuterium-labeled internal standard was extracted from plasma by liquid-liquid extraction with 1 mL n-hexane/ethyl acetate (3:2, v/v). Reversed-phase chromatography and positive electrospray ionization were used in the ID-LC-MS/MS. Gradient elution coupled with use of a C18-packed ultrahigh-performance liquid chromatography column allowed complete baseline resolution of 17-OHP from its structural analogue desoxycorticosterone in 6 min. To determine the 17-OHP level in human plasma, a bracketing calibration method was used to give higher accuracy and precision. The limit of detection and the lower limit of the measuring interval for the candidate RMP were 2.1 pg/mL (6.4 pmol/L) and 4.6 pg/mL (13.9 pmol/L), respectively. Extraction recovery was determined to be (96.08 ± 3.03)% (n = 3). Imprecision (intra-assay and interassay) was 4.03% or less at 0.83, 15.19, 64.22, and 313.46 ng/mL (2.51, 45.97, 194.34, and 948.56 nmol/L, respectively). Recoveries ranged from 98.05% to 102.24%. When comparing our RMP results with those obtained with an established RMP via International Federation of Clinical Chemistry and Laboratory Medicine external quality assessment scheme for reference laboratories in laboratory medicine (RELA) samples, we found that the biases ranged from -1.99% to 3.08% against the targets. No interference was observed, and the linear response ranged from 0.47 to 958.63 ng/mL (1.42 to 2900.90 nmol/L). Moreover, the candidate RMP was used to measure the concentration of 17-OHP in human plasma and was compared with an immunoassay using 40 plasma samples. The performance of the method meets the needs of an RMP (total coefficient of variation of 5% or less and bias of 3.08% or less). This method can be used for reference material value assignment of 17-OHP in human plasma matrix. It could also serve as an accurate reference baseline for routine methods to increase the accuracy and precision of certain clinical laboratory measurements. Graphical abstract Selected ion chromatograms obtained by liquid chromatography-tandem mass spectrometry with a C18 column for 17α-hydroxyprogesterone (17-OHP) from a plasma sample.
Assuntos
17-alfa-Hidroxiprogesterona/sangue , Cromatografia Líquida de Alta Pressão/métodos , Espectrometria de Massas em Tandem/métodos , Calibragem , Cromatografia Líquida de Alta Pressão/normas , Deutério/sangue , Humanos , Técnicas de Diluição do Indicador , Marcação por Isótopo , Padrões de Referência , Valores de Referência , Reprodutibilidade dos Testes , Espectrometria de Massas em Tandem/normasRESUMO
We explored a novel doubly labelled water (DLW) method based on breath water (BW-DLW) in mice to determine whole body CO2 production and energy expenditure noninvasively. The BW-DLW method was compared to the DLW based on blood plasma. Mice (n = 11, 43.5 ± 4.6â g body mass (BM)) were administered orally a single bolus of doubly labelled water (1.2â g H218O kg BM-1 and 0.4â g 2H2O kg BM-1, 99 atom% (AP) 18O or 2H). To sample breath water, the mice were placed into a respiration vessel. The exhaled water vapour was condensed in a cold-trap. The isotope enrichments of breath water were compared with plasma samples. The 2H/1H and 18O/16O isotope ratios were measured by means of isotope ratio mass spectrometry. The CO2 production (RCO2) was calculated from the 2H and 18O enrichments in breath water and plasma over 5 days. The isotope enrichments of breath water vs. plasma were correlated (R2 = 0.89 for 2H and 0.95 for 18O) linearly. The RCO2 determined based on breath water and plasma was not different (113.2 ± 12.7 vs. 111.4 ± 11.0â mmolâ d-1), respectively. In conclusion, the novel BW-DLW method is appropriate to obtain reliable estimates of RCO2 avoiding blood sampling.
Assuntos
Bioquímica/métodos , Testes Respiratórios/métodos , Dióxido de Carbono/análise , Metabolismo Energético , Animais , Bioquímica/instrumentação , Testes Respiratórios/instrumentação , Dióxido de Carbono/metabolismo , Deutério/análise , Deutério/sangue , Feminino , Marcação por Isótopo/métodos , Cinética , Masculino , Espectrometria de Massas , Camundongos , Oxigênio/análise , Isótopos de Oxigênio/análise , Isótopos de Oxigênio/sangue , VaporRESUMO
In the clinical laboratories, dehydroepiandrostenedione (DHEA) is usually quantified by immunoassay-based methods, which are often affected by cross-reactivity with endogenous interferences, such as 4-androsten-3ß-ol-17-one. The interfering compounds lead to a poor accuracy of the measurements, mainly at a low concentration level. The present paper describes a validated method based on tandem mass spectrometry coupled to liquid chromatography, for the accurate quantification of DHEA in serum. The peculiarity of this method is the use of calibrators and quality controls prepared by adding measured amounts of DHEA-D5, a stable isotope-labeled analogue of DHEA, to real serum from healthy subjects. DHEA-D5 is used in place of DHEA, which is usually present in unstripped serum at physiological levels, as it has the same basic structure, provides an equivalent instrumental response, and can be easily distinguish by DHEA by mass spectrometry due to its different m/z value. The method proved to be sensitive, with a LLOD of 0.09 ng/mL and a LLOQ of 0.23 ng/mL, and selective, with overall performances that allow its use on a routine basis.
Assuntos
Desidroepiandrosterona/sangue , Espectrometria de Massas em Tandem/métodos , Calibragem , Cromatografia Líquida de Alta Pressão/métodos , Desidroepiandrosterona/análogos & derivados , Deutério/análise , Deutério/sangue , Humanos , Cinética , Limite de DetecçãoRESUMO
GC-MS and GC-MS/MS methods were developed and validated for the quantitative determination of ibuprofen (d0-ibuprofen), a non-steroidal anti-inflammatory drug (NSAID), in human plasma using α-methyl-2H3-4-(isobutyl)phenylacetic acid (d3-ibuprofen) as internal standard. Plasma (10µL) was diluted with acetate buffer (80µL, 1M, pH 4.9) and d0- and d3-ibuprofen were extracted with ethyl acetate (2×500µL). After solvent evaporation d0- and d3-ibuprofen were derivatized in anhydrous acetonitrile by using pentafluorobenzyl (PFB) bromide and N,N-diisopropylethylamine as the base catalyst. Under electron-capture negative-ion chemical ionization (ECNICI), the PFB esters of d0- and d3-ibuprofen readily ionize to form their carboxylate anions [M-PFB]- at m/z 205 and m/z 208, respectively. Collision-induced dissociation (CID) of m/z 205 and m/z 208 resulted in the formation of the anions at m/z 161 and m/z 164, respectively, due to neutral loss of CO2 (44 Da). A collision energy-dependent H/D isotope effect was observed, which involves abstraction/elimination of H- from d0-ibuprofen and D- from d3-ibuprofen and is minimum at a CE value of 5eV. Quantitative GC-MS determination was performed by selected-ion monitoring of m/z 205 and m/z 208. Quantitative GC-MS/MS determination was performed by selected-reaction monitoring of the mass transitions m/z 205 to m/z 161 for d0-ibuprofen and m/z 208 to m/z 164 for d3-ibuprofen. In a therapeutically relevant concentration range (0-1000µM) d0-ibuprofen added to human plasma was determined with accuracy (recovery, %) and imprecision (relative standard deviation, %) ranging between 93.7 and 110%, and between 0.8 and 4.9%, respectively. GC-MS (y) and GC-MS/MS (x) yielded almost identical results (y=4.00+0.988x, r2=0.9991). In incubation mixtures of arachidonic acid (10µM), d3-ibuprofen (10µM) or d0-ibuprofen (10µM) with ovine cyclooxygenase (COX) isoforms 1 and 2, the concentration of d3-ibuprofen and d0-ibuprofen did not change upon incubation at 37°C up to 60min. The trough pharmacokinetics of an inhaled arginine-containing ibuprofen preparation in mice was studied after once-daily treatment (0.0, 0.07, 0.4 and 2.5mg/kg body weight) for three days. A linear relationship between ibuprofen concentration in serum (10µL) and administered dose 24h after the last drug administration was observed.
Assuntos
Cromatografia Gasosa-Espectrometria de Massas/métodos , Ibuprofeno/sangue , Ibuprofeno/isolamento & purificação , Extração Líquido-Líquido/métodos , Espectrometria de Massas em Tandem/métodos , Acetatos , Animais , Deutério/sangue , Deutério/química , Deutério/metabolismo , Feminino , Fluorbenzenos , Humanos , Ibuprofeno/química , Ibuprofeno/metabolismo , Limite de Detecção , Modelos Lineares , Camundongos , Camundongos Endogâmicos BALB C , Reprodutibilidade dos TestesRESUMO
RATIONALE: Studies of molecular biodegradation by mass spectrometry often require synthetic compounds labeled with stable isotopes as internal standards. However, labeling is very expensive especially when a large number of compounds are needed for analysis of biotransformation. Here we describe an approach for qualitative and quantitative analysis using bradykinin (BK) and its in vitro degradation metabolites as an example. Its novelty lies in the use of deuterated peptides which are obtained by a high-temperature solid-state exchange (HSCIE) reaction. METHODS: Deuterated and native BK were analyzed by positive electrospray ionization high-resolution mass spectrometry (ESI-HRMS) using an Orbitrap Fusion mass spectrometer. High-energy collision-induced dissociation (HCD) experiments were performed on [M+H](+) and [M+2H](2+) ions in targeted-MS(2) mode with adjusted normalized HCD value. RESULTS: After the HSCIE reaction, each amino acid residue of the deuterated peptide contained deuterium atoms and the average degree of substitution was 5.5 atoms per the peptide molecule. The deuterated peptide demonstrated the same chromatographic mobility as the unlabeled counterpart, and lack of racemization during substitution with deuterium. Deuterium-labeled and unlabeled BKs were incubated with human plasma and their corresponding fragments BK(1-5) and BK(1-7), well known as the major metabolites, were detected. CONCLUSIONS: Quantitative assays demonstrated applicability of the heavy peptide for both sequencing and quantification of generated fragments. Applicability of the HSCIE deuterated peptide for analysis of routes of its degradation has been shown in in vitro experiments. Copyright © 2016 John Wiley & Sons, Ltd.
Assuntos
Bradicinina/sangue , Bradicinina/metabolismo , Espectrometria de Massas por Ionização por Electrospray/métodos , Biotransformação , Bradicinina/análise , Linhagem Celular , Deutério/análise , Deutério/sangue , Deutério/metabolismo , Medição da Troca de Deutério/métodos , Temperatura Alta , HumanosRESUMO
Naturally occurring deuterium ((2)H) in biota can be used to trace movement, migration and geographic origin of a range of organisms. However, to evaluate movements of animals using δ(2)H measurements of tissues, it is necessary to establish the turnover time of (2)H in the tissues and the extent of isotopic discrimination from different environmental (2)H sources to those tissues. We investigated the turnover of (2)H in lake sturgeon (Acipenser fulvescens) blood by manipulating both environmental water δ(2)H and diet δ(2)H over a four-month period. The half-life of deuterium in lake sturgeon blood was 37.9 days after an increase in the environmental water δ(2)H of +714â . However, no clear turnover in blood (2)H occurred over the same period in a separate trial following a change of -63.8â or +94.2â in diet. These findings suggest that environmental water (2)H exchanges much faster with blood than diets and that blood δ(2)H values can be used to trace movements of sturgeon and other fish moving among isotopically distinct waters.
Assuntos
Migração Animal , Deutério/sangue , Peixes/sangue , Animais , Dieta , Peixes/fisiologiaRESUMO
Incorporation of deuterium from deuterium oxide ((2) H2 O) into biological components is a commonly used approach in metabolic studies. Determining the dilution of deuterium in the body water (BW) pool can be used to estimate body composition. We describe three sensitive GC/MS/MS methods to measure water enrichment in BW. Samples were reacted with NaOH and U-(13) C3 -acetone in an autosampler vial to promote deuterium exchange with U-(13) C3 -acetone hydrogens. Headspace injections were made of U-(13) C3 -acetone-saturated air onto a 30-m DB-1MS column in electron impact-mode. Subjects ingested 30 ml (2) H2 O, and plasma samples were collected. BW was determined by standard equation. Dual-energy X-ray absorptiometry scans were performed to calculate body mass, body volume and bone mineral content. A four-compartmental model was used to estimate body composition (fat and fat free mass). Full-scan experiments generated an m/z 45 peak and to a lesser extent an m/z 61 peak. Product fragment ions further monitored included 45 and 46 using selected ion monitoring (Method1), the 61 > 45 and 62 > 46 transition using multiple reaction monitoring (MRM; Method2) and the neutral loss, 62 > 45, transition (Method3). MRM methods were optimized for collision energy (CE) and collision-induced dissociation (CID) argon gas pressure with 6 eV CE and 1.5 mTorr CID gas being optimal. Method2 was used for final determination of (2) H2 O enrichment of subjects because of lower natural background. We have developed a sensitive method to determine (2) H2 O enrichment in BW to enable measurement of FM and FFM.
Assuntos
Água Corporal/química , Óxido de Deutério/química , Deutério/sangue , Cromatografia Gasosa-Espectrometria de Massas/métodos , Acetona , Adulto , Idoso , Água Corporal/metabolismo , Deutério/metabolismo , Óxido de Deutério/metabolismo , Humanos , Pessoa de Meia-Idade , Espectrometria de Massas em Tandem/métodos , Adulto JovemRESUMO
It is found that consumption of deuterium depleted water reduces not only the content of deuterium in biological fluids but also more than 2 times reduces the D/H gradient value along the line: mixed saliva > blood plasma. The experimental data showed that a physiological solution prepared on deuterium depleted water during induced apoptosis activates the DNA repair system, significantly reducing the number of single-stranded DNA breaks, which, in general, indicates an increase in the efficiency of defensive systems of the cell.
Assuntos
Adaptação Fisiológica/efeitos dos fármacos , Deutério/farmacologia , Animais , Apoptose , Dano ao DNA , Deutério/sangue , Deutério/farmacocinética , Humanos , Rim/metabolismo , Fígado/metabolismo , Miocárdio/metabolismo , Ratos , Saliva/metabolismo , Distribuição TecidualRESUMO
With the use of nuclear magnetic resonance we investigated the effect of consumption of water with the modified isotope content on the composition of deuterium in the blood, assessed the state of pro-oxidant-antioxidant system in the blood and lyophilized tissues (liver, kidneys) under prolonged oxidative stress. Possible mechanisms of direct and indirect antioxidant effects of the water with modified isotope content and the perspectives of its use for nutritional correction of abnormalities of oxidative metabolism during special physiological conditions and in clinical practice are discussed.
Assuntos
Antioxidantes/metabolismo , Deutério , Radicais Livres/metabolismo , Águas Minerais , Estresse Oxidativo , Infecções dos Tecidos Moles/sangue , Animais , Deutério/análise , Deutério/sangue , Deutério/farmacologia , Rim/metabolismo , Fígado/metabolismo , Espectroscopia de Ressonância Magnética , Masculino , Águas Minerais/administração & dosagem , Águas Minerais/análise , RatosRESUMO
Stable hydrogen isotopes (δ(2)H) are commonly used in studies of animal movement. Tissue that is metabolically inactive after growth (e.g., feathers) provides spatial or dietary information that reflects only the period of tissue growth, whereas tissues that are metabolically active (e.g., red blood cells) provide a moving window of forensic information. However, using δ(2)H for studies of animal movement relies on the assumption that tissue δ(2)H values reflect dietary δ(2)H values, plus or minus a net diet-tissue discrimination value, and that the turnover rate is known for metabolically active tissue. The metabolic rate of an animal may influence both diet-tissue discrimination values and isotopic tissue turnover rate, but this hypothesis has not been tested experimentally. To examine the metabolic hypothesis, an experimental group of 12 male and 15 female captive Japanese quail (Coturnix japonica) was housed at 8.9°C for 90 d to elevate their metabolic rates (mL CO(2) min(-1)), and a control group of 12 male and 13 female quail was housed at room temperature during the same period. For both experimental and control birds, diet-tissue discrimination values were estimated for red blood cells and feathers. To determine turnover rate, experimental and control birds were switched from a (2)H-enriched diet to a (2)H-depleted diet, with red blood cells sampled before and after diet switch. Metabolic rate did not influence red blood cell hydrogen isotope turnover rate (η(2)(p) = 0.24)) or diet-feather isotope discrimination values (η(2)(p) = 0.86). Diet-feather hydrogen isotopic discrimination had a significant sex plus treatment interaction effect; female feathers were depleted in (2)H relative to food regardless of treatment, whereas male feathers were enriched in (2)H. The effect of sex suggested that experimental studies should examine whether coeval males and females differ in blood δ(2)H levels during certain periods of the annual cycle.
Assuntos
Coturnix/metabolismo , Deutério/farmacocinética , Ração Animal/análise , Animais , Metabolismo Basal , Temperatura Baixa , Coturnix/sangue , Deutério/administração & dosagem , Deutério/sangue , Eritrócitos/metabolismo , Plumas/metabolismo , Feminino , Masculino , Distribuição Aleatória , Distribuição por Sexo , Distribuição TecidualRESUMO
The analysis of hydrogen stable isotopes (δD) is a potentially powerful tool for studying animal ecology. Unlike other stable isotopes used in ecological research, however, we are less familiar with the physiological processes that influence the incorporation of hydrogen isotopes from dietary resources to animal tissues. Here we present the results of a controlled feeding experiment utilizing Japanese quail (Cortunix japonica) that was designed to: (1) estimate the relative contributions of diet to the δD signatures of blood plasma, red blood cells, intestine, liver, muscle and feathers; (2) investigate possible differences among these same tissues in diet to tissue discrimination; and (3) explore the differences in incorporation dynamics between deuterium ((2)H) and a well-studied isotope, (13)C, for blood plasma solids and red blood cells. Tissues differed in both the relative contribution of diet to tissue δD and diet to tissue discrimination. The average residence time of both hydrogen and carbon was significantly lower in plasma solids than in red blood cells. The average residence time of hydrogen was significantly lower than that of carbon in plasma solids, but not in red blood cells. Although the average residence times of hydrogen and carbon were positively correlated, the correlation was weak. Hence the incorporation of carbon seems to be a poor predictor of that of hydrogen.
Assuntos
Coturnix/metabolismo , Deutério/farmacocinética , Animais , Isótopos de Carbono/administração & dosagem , Isótopos de Carbono/sangue , Isótopos de Carbono/farmacocinética , Coturnix/sangue , Deutério/administração & dosagem , Deutério/sangue , Dieta , Eritrócitos/metabolismo , Feminino , Masculino , Plasma/metabolismo , Distribuição TecidualRESUMO
We investigated the relationships between the δdeuterium (δD) and the δ(18)oxygen (δ(18)O) of drinking water and the δD and δ(18)O of blood plasma, red blood cells and feathers in house sparrows (Passer domesticus) fed on diets with identical hydrogen and oxygen isotopic compositions and five isotopically distinct drinking water treatments. We expected and, with only one exception ((18)O in blood plasma), found linear relationships between the δD and δ(18)O values of drinking water and those of bird tissues. The slopes of these relationships, which estimate the percentage contributions of drinking water to the tissue isotopic signatures, were lower than those of previous studies. We found significant differences in the δD and δ(18)O values of feathers, red blood cells and plasma solids. In feathers and red blood cells, δD and δ(18)O values were linearly correlated. Our results have three implications for isotopic field studies: (1) if the isotopic composition of drinking water differs from that of food, its effect on tissue isotope values can confound the assignment of animals to a site of origin; (2) comparisons of the δD and δ(18)O values of different tissues must account for inter-tissue discrimination factors; and (3) δD/δ(18)O linear relationships are probably as prevalent in animal systems as they are in geohydrological systems. These relationships may prove to be useful tools in animal isotopic ecology.
Assuntos
Deutério/sangue , Plumas/química , Isótopos de Oxigênio/sangue , Pardais/sangue , Abastecimento de Água/análise , Análise de Variância , Animais , Deutério/análise , Isótopos de Oxigênio/análise , Pardais/metabolismo , WyomingRESUMO
Bisphenol A (BPA) is an important industrial chemical used in the manufacture of polycarbonate plastic products, epoxy resin-based food can liners, and paper products. The presence of BPA in urine of >90% of Americans aged 6-60 suggests ubiquitous and frequent exposure and is problematic because of the potential for endocrine disruption. The ubiquity of environmental BPA in common laboratory supplies used for sample collection, storage, and analysis greatly increases the likelihood of false positive determinations, particularly at trace levels. The current study validated using liquid chromatography/tandem mass spectrometry (LC/MS/MS) in conjunction with deuterated BPA as the dosing material to circumvent contamination for high sensitivity quantifications in rat serum, tissues, urine, and feces. The methods described provided measurements of both estrogen receptor-active aglycone and metabolically deactivated conjugated forms of BPA, a distinction that is critical to assessing toxicological potential. The adequacy of the described methodology was substantiated by its utility in analyzing samples from rats treated orally with a 100 µg/kg body weight dose of d6-BPA. These results emphasize the challenges inherent in measuring BPA in biological samples and how employing stable isotope labeled dosing can facilitate pharmacokinetic studies needed to understand BPA metabolism and disposition. Such studies conducted in experimental animal models, in conjunction with properly validated human biomonitoring data, will be the basis for PBPK modeling of BPA in environmentally exposed humans.
Assuntos
Cromatografia Líquida/métodos , Deutério/análise , Fezes/química , Fenóis/análise , Espectrometria de Massas em Tandem/métodos , Animais , Compostos Benzidrílicos , Técnicas de Laboratório Clínico/normas , Deutério/sangue , Deutério/química , Deutério/urina , Feminino , Fenóis/sangue , Fenóis/química , Fenóis/urina , Ratos , Ratos Sprague-Dawley , Padrões de Referência , Reprodutibilidade dos TestesRESUMO
Homocysteine plays a key role in several pathophysiological conditions. To assess the methionine-homocysteine kinetics by stable isotope methodology, we developed a simultaneous quantification method of [(2)H(7)]methionine, [(2)H(4)]methionine, methionine, [(2)H(4)]homocysteine and homocysteine in rat plasma by gas chromatography-mass spectrometry (GC-MS). [(13)C]Methionine and [(13)C]homocysteine were used as analytical internal standards to account for losses associated with the extraction, derivatization and chromatography. For labeled and non-labeled homocysteine measurements, disulfide bonds between homocysteine and other thiols or proteins were reduced by dithiothreitol. The reduced homocysteine and methionine species were purified by cation-exchange chromatography and derivatized with isobutyl chlorocarbonate in water-ethanol-pyridine. Quantification was carried out by selected ion monitoring of the molecular-related ions of N(O,S)-isobutyloxycarbonyl ethyl ester derivatives on the chemical ionization mode. The intra- and inter-day precision of the assay was less than 6% for all labeled and non-labeled methionine and homocysteine species. The method is sensitive enough to determine pharmacokinetics of labeled methionine and homocysteine.
Assuntos
Deutério/sangue , Cromatografia Gasosa-Espectrometria de Massas/métodos , Homocisteína/sangue , Metionina/sangue , Metionina/farmacocinética , Animais , Ésteres/química , Metionina/administração & dosagem , Ratos , Fatores de TempoRESUMO
BACKGROUND & AIMS: The intestine is a major site of amino acid metabolism, especially in neonates. Neonatal animals derive energy needed for metabolic processes from dietary glucose and amino acids. Rats were found to oxidize non-essential amino acids such as aspartate, glutamate and glutamine in the intestine at a high rate. We have previously found that glutamate and glucose are important sources of energy for the splanchnic tissues in fully fed preterm infants. However, no data are available on splanchnic aspartate metabolism in human preterm infants. In the present study we studied whole-body and splanchnic aspartate metabolism and determined the metabolic fate of aspartate. METHODS: In eight, enterally fed, preterm infants (gestational age 31 weeks (wk)+/-3 SD, range: 26-34wk) splanchnic and whole-body aspartate kinetics were assessed by dual tracer ([U-(13)C]aspartate and [D(3)]aspartate) techniques. RESULTS: Splanchnic first-pass aspartate uptake was almost complete (77+/-15%). Almost all (80+/-9%) of the (13)C administered as [U-(13)C]aspartate used in first-pass was recovered as CO(2) in expired breath. CONCLUSION: The splanchnic tissues extract almost all of the dietary aspartate in preterm infants. The majority of the labeled carbon is recovered in expired breath, making it most likely that the sequestered carbon skeleton of aspartate is utilized for energy generation.
Assuntos
Ácido Aspártico/administração & dosagem , Ácido Aspártico/metabolismo , Nutrição Enteral , Recém-Nascido Prematuro/metabolismo , Absorção Intestinal , Ácido Aspártico/sangue , Testes Respiratórios , Dióxido de Carbono/análise , Isótopos de Carbono/administração & dosagem , Isótopos de Carbono/análise , Isótopos de Carbono/sangue , Deutério/administração & dosagem , Deutério/análise , Deutério/sangue , Metabolismo Energético , Humanos , Técnicas de Diluição do Indicador , Recém-Nascido de Baixo Peso/sangue , Recém-Nascido de Baixo Peso/metabolismo , Recém-Nascido , Recém-Nascido Prematuro/sangue , Infusões Intravenosas , Cinética , Bicarbonato de Sódio/administração & dosagem , Bicarbonato de Sódio/metabolismo , Circulação EsplâncnicaRESUMO
This study describes a GC-MS method for the simultaneous determination of androstenedione (AD), 11beta-hydroxyandrostenedione (11beta-OHAD), and testosterone (TS) in human plasma. [19,19,19-(2)H(3)]Androstenedione (AD-(2)H(3)), 11beta-hydroxy-[1,2,4,19-(13)C(4)]androstenedione (11beta-OHAD-(13)C(4)), and [1,16,16,17-(2)H(4)]testosterone (TS-(2)H(4)) were used as internal standards. Pentafluoropropionic (PFP) derivatization with good GC behavior was employed for the GC-MS analysis of the three steroids. The detection limit of the present GC-MS-SIM method was found to be 1 pg per injection for AD (S/N ratio=4.5), 5 pg for 11beta-OHAD (S/N ratio=5.0), and 1 pg for TS (S/N ratio=4.4), respectively. Calibration curves were linear from 0.22 to 2.80 ng/mL (r=0.9998) for AD, from 0.56 to 3.19 ng/mL (r=0.9996) for 11beta-OHAD, and from 2.05 to 10.3 ng/mL (r=0.9996) for TS. The intra- and inter-day assay reproducibilities in the amounts of the three androgens determined were in good agreement with the actual amounts added, the relative errors (R.E.) were -3.1 to 2.4%. The inter-assay relative standard deviation (R.S.D.) was less than 5.3%. The present method provides a sensitive and reliable technique for the simultaneous determination of AD, 11beta-OHAD, and TS in plasma. The method can be applied to pharmacokinetic and metabolic studies of androgens with a particular interest in evaluating the conversion of AD to 11beta-OHAD and the interconversion of AD and TS in humans.
Assuntos
Androstenodiona/sangue , Isótopos de Carbono/sangue , Deutério/sangue , Espectrometria de Massas/métodos , Testosterona/sangue , Androstenodiona/química , HumanosRESUMO
In doubly labelled water studies, biological sample enrichments are mainly measured using off-line techniques (equilibration followed by dual-inlet introduction) or high-temperature elemental analysis (HT-EA), coupled with an isotope-ratio mass spectrometer (IRMS). Here another continuous-flow method, (CF-EA/IRMS), initially dedicated to water, is tested for plasma and urine analyses. The elemental analyser configuration is adapted for each stable isotope: chromium tube for deuterium reduction and glassy carbon reactor for 18O pyrolysis. Before on-line conversion of water into gas, each matrix is submitted to a short and easy treatment, which is the same for the analysis of the two isotopes. Plasma is passed through centrifugal filters. Urine is cleaned with black carbon and filtered (0.45 microm diameter). Tested between 150 and 300 ppm in these fluids, the D/H ratio response is linear with good repeatability (SD<0.2 ppm) and reproducibility (SD<0.5 ppm). For 18O/16O ratios (from 2000 to 2200 ppm), the same repeatability is obtained with a between-day precision lower than 1.4 ppm. The accuracy on biological samples is validated by comparison to classical dual-inlet methods: 18O analyses give more accurate results. The data show that enriched physiological fluids can be successfully analysed in CF-EA/IRMS.
Assuntos
Deutério/química , Oxigênio/química , Água/química , Animais , Calibragem , Deutério/sangue , Deutério/urina , Humanos , Marcação por Isótopo , Espectrometria de Massas , Oxigênio/sangue , Oxigênio/urina , Isótopos de Oxigênio , Padrões de Referência , Reprodutibilidade dos TestesRESUMO
The dual-tracer method has been used conventionally for assessment of postprandial fluxes, i.e., appearance in plasma of ingested glucose (R(a meal)), endogenous glucose production (EGP), and disposal (R(d)). To quantify the magnitude of errors affecting the calculations and their dependence on model assumptions, this method was assessed and compared with the triple-tracer method, which provides model-independent estimates. For this purpose, the dual-tracer protocol was performed twice in eight normal subjects, with [1-(13)C]glucose to trace ingested glucose and [6,6-(2)H(2)]glucose constantly infused. A third tracer, [6-(3)H]glucose, was infused at variable rates to render the calculation of R(a meal) and EGP virtually model independent. The dual-tracer method analyzed with a one-compartment model performed poorly, since R(a meal) peak was significantly lower and delayed compared with triple-tracer reference, resulting in a significantly lower estimation of the amount of absorbed glucose (9,036 +/- 558 vs. 11,316 +/- 823 micromol/kg, P = 0.0117). EGP showed a paradoxical pattern, with an initial overshoot followed by a rapid decay to negative values, resulting in a significant underestimation of EGP suppression (57 +/- 3 vs. 65 +/- 4%, P = 0.0117). A two-compartment model performed better but did not overcome the limitations of the dual-tracer approach, since the amount of absorbed glucose was still significantly underestimated (10,231 +/- 661 vs. 12,169 +/- 838 micromol/kg, P = 0.0117) and EGP still showed a paradoxical behavior. R(d), estimated from R(a meal) and EGP, was significantly underestimated with the dual-tracer method, irrespective of adopted model. We conclude that three suitably infused tracers are required for accurate assessment of postprandial R(a meal), EGP, and R(d).