RESUMO
PURPOSE: This study aimed to investigate the frequency of Enterocytozoon bieneusi and Encephalitozoon intestinalis in patients with diarrhea in the immunosuppressed. METHODS: Patients between the ages of 18-85 who applied to different clinics of Mus Bulanik and Bitlis State Hospitals and were referred to the microbiology or parasitology laboratory were selected for this study. A total of 200 individuals, including 88 immunosuppressed with diarrhea patients, 38 immunocompetent with diarrhea patients, 38 immunosuppressed without diarrhea patients, and 36 immunocompetent without diarrhea individuals, were included. Collected stool samples were evaluated using IFA-MAbs and real-time PCR methods to determine the frequency of E.intestinalis and E.bieneusi. RESULTS: E. intestinalis was detected in 59 (29.5%) of 200 samples and E. bieneusi was detected in 46 (23.0%) of them. Mixed infection was detected in 16 (8%) of the positive samples. While there was no statistically significant difference between E. intestinalis positivity and gender, age, diarrhea status and immune system status, a statistically significant relationship was determined between E. bieneusi positivity and diarrhea. When the real-time PCR method was accepted as the gold standard, the sensitivity of the IFA-MAbs method in the diagnosis of E. intestinalis was 94.54%, the specificity was 97.24, the sensitivity in the diagnosis of E. bieneusi was 95.45%, and the specificity was 98.72%. The overall accuracy of the IFA-MAbs method was 96.5% for the diagnosis of E. intestinalis and 98% for the diagnosis of E. bieneusi. CONCLUSIONS: The findings suggest that E. intestinalis and E. bieneusi should be considered in both immunosuppressed and healthy individuals with diarrhea. IFA-MAbs method can be used in addition to the real-time PCR method to diagnose E. intestinalis and E. bieneusi.
Assuntos
Encefalitozoonose , Enterocytozoon , Microsporidiose , Animais , Camundongos , Humanos , Adolescente , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Encefalitozoonose/diagnóstico , Encefalitozoonose/microbiologia , Reação em Cadeia da Polimerase em Tempo Real , Enterocytozoon/genética , Microsporidiose/diagnóstico , Microsporidiose/epidemiologia , Fezes/microbiologia , Diarreia/diagnóstico , Anticorpos MonoclonaisRESUMO
BACKGROUND: There is a relative paucity of data examining the prevalence of renal pathology in wild rabbits. METHODS: Sixty-two wild rabbits that had been shot for population control in Cambridgeshire, UK, underwent postmortem examination, including macroscopic and microscopic renal assessment. RESULTS: The majority (82%) of the animals had macroscopically and microscopically normal kidneys. One animal (1.6%) had severe perirenal abscessation. Pasteurella spp. was isolated from this lesion. Ten rabbits (16%) had microscopic renal pathology comprising minimal to mild renal inflammation or fibrosis. No Encephalitozoon cuniculi organisms were detected histologically. LIMITATIONS: The sample population was composed of shot rabbits, so the probability of detecting moribund individuals was reduced. Extrapolation of these data to the wider UK wild rabbit population may be limited as rabbits were shot at two sites within a 3 km radius of each other. CONCLUSION: Renal pathology is rare in the population examined.
Assuntos
Encephalitozoon cuniculi , Encefalitozoonose , Animais , Coelhos , Rim , Encéfalo/patologia , Encefalitozoonose/epidemiologia , Encefalitozoonose/veterinária , Encefalitozoonose/diagnósticoRESUMO
A 2-year-old female mixed-breed canine patient from Namibia presented originally with chronic uveitis. A serum antibody titer and a PCR test performed on the aqueous humor were positive for encephalitozoon cuniculi. The left eye showed an immature anterior focal cortical cataract in the periphery with suspected lens capsule rupture and signs of chronic uveitis. An incipient anterior focal cortical cataract was also perceivable in the patient's right eye. Despite local treatment as well as systemic administration of carprofen, prednisolone, and fenbendazol recurrent uveitis occurred. The patient then underwent bilateral extracapsular lensextraction via phacoemulsification. A PCR test of the lens material was positive for encephalitozoon cuniculi strain III. Recurring uveitis and secondary glaucoma 10 months post-op resulted in permanent blindness of the left eye. The patient then continued to receive local anti-inflammatory treatment. The last recheck examination of both eyes, 31 month post-op, revealed no signs of uveitis. This is the first case reported of a cataract in a canine patient caused by encephalitozoon cuniculi strain III.
Assuntos
Catarata , Doenças do Cão , Encephalitozoon cuniculi , Encefalitozoonose , Uveíte , Feminino , Animais , Cães , Encefalitozoonose/diagnóstico , Encefalitozoonose/tratamento farmacológico , Encefalitozoonose/veterinária , Melhoramento Vegetal , Catarata/complicações , Catarata/diagnóstico , Catarata/veterinária , Uveíte/diagnóstico , Uveíte/tratamento farmacológico , Uveíte/veterinária , Doenças do Cão/diagnóstico , Doenças do Cão/tratamento farmacológicoRESUMO
Encephalitozoon cuniculi is a microsporidian parasite mostly associated with its natural host, the rabbit (Oryctolagus cuniculus). However, other animals can be infected, like other mammals, birds, and even humans. Although it usually causes subclinical infection, it can also lead to encephalitozoonosis, a clinical disease characterized by neurological, ocular, and/or renal signs that can be even fatal, especially in immunocompromised individuals. Therefore, this multidisciplinary review contributes with updated information about the E. cuniculi, deepening in its molecular and genetic characterization, its mechanisms of infection and transmission, and its prevalence among different species and geographic locations, in a One Health perspective. Recent information about the diagnostic and therapeutic approach in the main host species and the prophylaxis and infection control measures currently suggested are also discussed.
Assuntos
Encephalitozoon cuniculi , Encefalitozoonose , Saúde Única , Animais , Infecções Assintomáticas , Encephalitozoon cuniculi/genética , Encefalitozoonose/diagnóstico , Encefalitozoonose/epidemiologia , Encefalitozoonose/veterinária , Humanos , Hospedeiro Imunocomprometido , Mamíferos , CoelhosRESUMO
Encephalitozoon cuniculi is a microsporidian which is frequently reported from rabbits. This microorganism can either ravage rabbit farms or transmit to humans from pet rabbits. This study aimed to investigate the prevalence and the genotype distribution of E. cuniculi among pet rabbits. In this study urine samples were collected from 50 pet rabbits, aged 2 months to 3 years, admitted to teaching veterinary hospital. Four races Lop, Dutch, Mix, and Angora were screened for E. cuniculi. The clinical symptoms were recorded and total DNA was extracted from urine samples. E. cuniculi was identified using amplification of the small subunit ribosomal RNA (ssu rRNA) gene and its genotypes were characterized using PCR/sequencing of the polar tube protein (PTP) gene. Phylogenetic tree was drawn to confirm the characterized genotypes. Out of 50 samples, 41 (82 %) of rabbits were asymptomatic, while nine (18 %) had at least one of symptoms including head-tilt, circling, and ataxia. A statistical correlation was seen between mean age + SD and symptoms (P-value = 0.039). The presence of E. cuniculi was confirmed in 16/50 (32 %) rabbits and all of them were identified as the genotype I. Our findings represented no consistency between E. cuniculi PCR - positive and the presence of symptoms (P-value = 0.318). Our results showed positive correlation between symptoms and age; however, the lack of correlation between PCR results with age may signify the latent infection in younger rabbits. All identified E. cuniculi were the genotype I, which is reported from rabbits and humans, highlighting the zoonotic concern for this genotype, particularly among subjects who keep pet rabbits.
Assuntos
Encephalitozoon cuniculi , Encefalitozoonose , Animais , Encephalitozoon cuniculi/genética , Encefalitozoonose/diagnóstico , Encefalitozoonose/epidemiologia , Encefalitozoonose/veterinária , Genótipo , Filogenia , Reação em Cadeia da Polimerase/veterinária , CoelhosRESUMO
OVERVIEW: Encephalitozoon cuniculi is a common obligate intracellular microsporidian parasite of rabbits that is increasingly recognised as a pathogen of cats and other mammalian species. These guidelines aim to review the literature on feline E cuniculi infection and provide recommendations on prevention and management. INFECTION IN CATS: E cuniculi infection should be considered as a differential diagnosis in cases of feline uveitis and cataract formation. It is not significantly associated with either chronic kidney disease or meningoencephalitis. E cuniculi infection is more common in stray or feral cats than in pet cats. DIAGNOSIS AND TREATMENT: Serological tests for antibody detection in the blood are easy to perform and can be useful for diagnosis, but their specificity is low as antibodies have been found in apparently healthy cats. PCR appears to be more sensitive than histopathology for diagnosis, and is more sensitive when performed on cataractous lenses compared with aqueous humour, although ease of sampling is an obvious limitation. Treatment is with fenbendazole for 3 weeks and phacoemulsification to remove microsporidia from cataractous lenses. ZOONOTIC RISK: E cuniculi is a potential zoonotic agent, and there is a particular risk to immunocompromised humans posed by infected rabbits. Albeit infrequent, spore shedding has been identified in cats, so care should be taken around infected cats.
Assuntos
Doenças do Gato/terapia , Catarata/veterinária , Encephalitozoon cuniculi/fisiologia , Encefalitozoonose/veterinária , Uveíte/veterinária , Animais , Doenças do Gato/diagnóstico , Doenças do Gato/prevenção & controle , Catarata/diagnóstico , Catarata/parasitologia , Gatos , Diagnóstico Diferencial , Encefalitozoonose/diagnóstico , Encefalitozoonose/prevenção & controle , Encefalitozoonose/terapia , Uveíte/diagnóstico , Uveíte/parasitologiaRESUMO
OBJECTIVE: In recent years new infectious diseases, i.e. emerging or re-emerging diseases, have been coming to the forefront. Currently, microsporidia, considered to be a major cause of emerging and opportunistic infections particularly in immunocompromised individuals, are also included in this group. Therefore, the aim of our study was to map the prevalence of Encephalitozoon intestinalis and Enterocytozoon bieneusi infection in a group of patients and to compare it with the occurrence of specific antigens in immunocompetent people. METHODS: Detection of spores of both pathogens in faecal samples was performed by an immunofluorescence test using species-specific monoclonal antibodies. RESULTS: Positivity to E. intestinalis in 91 examined immunosuppressed patients reached 33% (30/91), while only 4.3% (3/70) of the control group samples were found to be positive (relative risk 7.7, p < 0.001). In case of E. bieneusi 14.3% (13/91) of immunocompromised patients were positive, as were 5.7% (4/70) of people from the control group (relative risk 2.5, p = 0.095). CONCLUSION: In case of development of any opportunistic infection, the infection is detected and removed in most cases at an early stage. The incidence of clinically manifested microsporidiosis in patients with immunodeficiency is rare as they are under constant medical supervision. However, we must not forget about opportunistic infections, and in case of any non-specific symptoms it is necessary to exclude or confirm the diagnosis for immediate treatment.
Assuntos
Encephalitozoon/isolamento & purificação , Encefalitozoonose/diagnóstico , Enterocytozoon/isolamento & purificação , Hospedeiro Imunocomprometido , Programas de Rastreamento , Microsporidiose/diagnóstico , Infecções Oportunistas/diagnóstico , Encefalitozoonose/epidemiologia , Fezes/microbiologia , Humanos , Microsporidiose/epidemiologia , Infecções Oportunistas/microbiologia , Eslováquia/epidemiologiaRESUMO
Encephalitozoon cuniculi, a zoonotic and opportunistic pathogen, can cause latent infection, especially in lagomorphs. Nowadays, this member of the Eukaryotes has drawn significant attention in the fields of veterinary and public health. The purpose of this study was to determine the seroprevalence of infection in a New Zealand rabbit farm that has a clinical history of neurological manifestations including head tilt ataxia, aggressiveness, seizures, and circling and rotational movements around the body length axis, but the general conditions and food intake were normal. Blood samples were taken from 42 breeding rabbits and researched for E. cuniculi antibodies. Out of that, 25 (59%) animals resulted positive against the pathogen. The rabbit was found to be seropositive for E. cuniculi antibodies, but negative for Toxoplasma gondii and Listeria monocytogenes antibodies. Hematological and serum biochemical parameters were measured at reference intervals. No brain tissue impairment was observed the computed tomography (CT) scan. As a result of these histopathological findings, the brain cortex presented severe neuronal degeneration and partial myelin loss, with reactive diffuse gliosis against the parasite spores was observed to the histopathology. These results are possibly related to the early stage of infection because the parasitic infestation comprise long time spreading. E. cuniculi DNA was detected on brain tissues using polymerase chain reaction (PCR), and it partial DNA sequence was identified as E. cuniculi genotype I.
Assuntos
Anticorpos Antifúngicos/sangue , Encephalitozoon cuniculi/imunologia , Encefalitozoonose/veterinária , Doenças do Sistema Nervoso/veterinária , Coelhos/microbiologia , Animais , Encéfalo/patologia , Encephalitozoon cuniculi/genética , Encephalitozoon cuniculi/isolamento & purificação , Encefalitozoonose/diagnóstico , Encefalitozoonose/microbiologia , Encefalitozoonose/patologia , Masculino , Doenças do Sistema Nervoso/diagnóstico , Doenças do Sistema Nervoso/microbiologia , Doenças do Sistema Nervoso/patologia , Neurônios/patologia , TurquiaRESUMO
INTRODUCTION: Encephalitozoon cuniculi is an obligate intracellular microsporidian parasite that commonly induces subclinical infections in rabbits, but occurs also in a range of other species, including various rodents, carnivores, humans and birds. The present report describes encephalitozoonosis in a group of captive Barbary striped grass mice (Lemniscomys barbarus) in a zoo collection. The aetiology was confirmed by immunohistochemistry and PCR with subsequent sequencing. The source of infection is not known.
Assuntos
Encephalitozoon cuniculi/isolamento & purificação , Encefalitozoonose/veterinária , Murinae/microbiologia , Animais , Animais de Zoológico/microbiologia , Encéfalo/microbiologia , DNA Fúngico/análise , DNA Fúngico/genética , Encefalitozoonose/diagnóstico , Encefalitozoonose/microbiologia , Coração/microbiologia , Imuno-Histoquímica , Reação em Cadeia da Polimerase , Baço/microbiologiaRESUMO
Encephalitozoon cuniculi is an obligate, intracellular microsporidian organism capable of establish infection in a wide variety of animals. In carnivores it may cause a sporadic, severe disease in the first few months of life, which usually culminates with the death of the animal. The objective of this study was to report a natural fatal case of encephalitozoonosis in a puppy from Argentina. Clinical signs included reduced appetite, depression, vocalizing, weight loss, weakness, convulsions and recumbency. No significant gross lesions were noticed at necropsy. Microscopically, severe, diffuse, lymphocytic encephalitis was seen. Large cytoplasmic vacuoles containing spores, morphologically compatible with E. cuniculi, were present within endothelial cells of brain and kidney, in renal tubular epithelium and hepatocytes. Encephalitozoon cuniculi DNA was detected by PCR in the kidney. Antibody titers to E. cuniculi in serum from the surviving puppies and the dam were ≥1:200. This report contributes to our understanding of neurologic disease in puppies. Encephalitozoonosis should be considered as a differential diagnosis in cases of fatal encephalitis in puppies.
Assuntos
Anticorpos Antifúngicos/sangue , Encéfalo/microbiologia , Doenças do Cão/microbiologia , Encefalitozoonose/veterinária , Fatores Etários , Animais , Encéfalo/patologia , Diagnóstico Diferencial , Doenças do Cão/diagnóstico , Cães , Encefalite/diagnóstico , Encephalitozoon cuniculi/genética , Encephalitozoon cuniculi/isolamento & purificação , Encefalitozoonose/diagnóstico , Evolução Fatal , Feminino , Rim/microbiologia , Rim/patologia , América LatinaRESUMO
Central vestibular dysfunction caused by Encephalitozoon cuniculi frequently mimics the condition of a peripheral disorder. A negative antibody titer rules out E cuniculi as the cause of present clinical signs. Cerebrospinal fluid analysis including polymerase chain reaction is considered an inappropriate diagnostic method for in vivo diagnosis of encephalitozoonosis. The usefulness of glucocorticoid anti-inflammatories in the treatment of encephalitozoonosis is called into question. Encouraging activity early in the course of disease and adding in therapeutic exercise may represent the most important part of therapy in rabbits with vestibular dysfunction associated with encephalitozoonosis.
Assuntos
Encephalitozoon cuniculi/isolamento & purificação , Encefalitozoonose/veterinária , Coelhos , Animais , Encefalitozoonose/diagnóstico , Encefalitozoonose/microbiologia , Encefalitozoonose/terapiaRESUMO
BACKGROUND: Microsporidia are intracellular obligate parasites traditionally associated with immunosuppressed patients; their detection in immunocompetent patients has increased, highlighting their possible importance as emerging pathogens. Detection of spores in stools, urine, body fluids and tissues is difficult and immunological techniques such as immunofluorescence have proved to be a useful and reliable tool in the diagnosis of human microsporidiosis. For this reason, we have produced and characterized monoclonal antibodies (MAbs) specific for Encephalitozoon intestinalis (the second most frequent microsporidian infecting humans), and other Encephalitozoon species, that can be used in different diagnostic techniques. RESULTS: Seven MAbs were selected in accordance with their optical density (OD). Four (4C4, 2C2, 2E5 and 2H2) were isotype IgG2a; two (3A5 and 3C9) isotype IgG3, and one Mab, 1D7, IgM isotype. The selected monoclonal antibody-secreting hybridomas were characterized by indirect immunofluorescence antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA), Western blot, immunoelectron microscopy (Immunogold) and in vitro cultures. The study by IFAT showed different behavior depending on the MAbs studied. The MAbs 4C4, 2C2, 2E5 and 2H2 showed reactivity against epitopes in the wall of the spore (exospore and endospore) epitopes located in Encephalitozoon sp. spores, whereas the MAbs 3A5, 1D7 and 3C9 showed reactivity against internal epitopes (cytoplasmic contents or sporoplasm) of E. intestinalis spores. All MAbs recognized the developing parasites in the in vitro cultures of E. intestinalis. Additionally, 59 formalin-fixed stool samples that had been previously analyzed were screened, with 26 (44%) presenting microsporidian spores (18 samples with E. intestinalis and 8 samples with Enterocytozoon bieneusi). Detection of microsporidian spores by microscopy was performed using Calcofluor stain, Modified Trichrome, Quick-Hot Gram Chromotrope, as well as IFAT using MAbs 4C4, 2C2, 2E5 and 2H2. The 4 MAbs tested clearly recognized the larger spores corresponding to E. intestinalis, but showed no reactivity with Enterocytozoon bieneusi spores. The mass spectrometry and proteomic study revealed that the Mabs 4C4, 2C2, 2E5 and 2H2 recognized the Spore Wall Protein 1 (SWP1) as the antigenic target. CONCLUSIONS: The IFAT-positive MAbs exhibited excellent reactivity against spores and developmental stages, permitting their use in human and animal diagnosis. The epitopes recognized (exospore, endospore and cytoplasmic contents) by the different MAbs developed need further study, and may reveal potential targets for vaccine development, immunotherapy and chemotherapy.
Assuntos
Anticorpos Monoclonais/imunologia , Encephalitozoon/imunologia , Esporos Fúngicos/crescimento & desenvolvimento , Esporos Fúngicos/imunologia , Anticorpos Monoclonais/química , Anticorpos Monoclonais/isolamento & purificação , Western Blotting , Encephalitozoon/isolamento & purificação , Encephalitozoon/fisiologia , Encefalitozoonose/diagnóstico , Encefalitozoonose/imunologia , Encefalitozoonose/microbiologia , Enterocytozoon/imunologia , Enterocytozoon/isolamento & purificação , Enterocytozoon/fisiologia , Fezes/microbiologia , Imunofluorescência , Humanos , Espectrometria de Massas/métodos , Microscopia , Microsporidiose/diagnóstico , Microsporidiose/imunologia , Microsporidiose/microbiologia , Proteômica/métodos , Esporos Fúngicos/isolamento & purificação , Esporos Fúngicos/ultraestruturaRESUMO
Rabbit medicine has been continuously evolving over time with increasing popularity and demand. Tremendous advances have been made in rabbit medicine over the past 5 years, including the use of imaging tools for otitis and dental disease management, the development of laboratory testing for encephalitozoonosis, or determination of prognosis in rabbits. Recent pharmacokinetic studies have been published, providing additional information on commonly used antibiotics and motility-enhancer drugs, as well as benzimidazole toxicosis. This article presents a review of evidence-based advances for liver lobe torsions, thymoma, and dental disease in rabbits and controversial and new future promising areas in rabbit medicine.
Assuntos
Prática Clínica Baseada em Evidências/normas , Coelhos , Medicina Veterinária/normas , Animais , Encefalitozoonose/diagnóstico , Encefalitozoonose/veterinária , Hepatopatias/diagnóstico , Hepatopatias/veterinária , Otite/diagnóstico , Otite/veterinária , Doenças Estomatognáticas/diagnóstico , Doenças Estomatognáticas/terapia , Doenças Estomatognáticas/veterinária , Timoma/cirurgia , Timoma/veterináriaAssuntos
Doenças do Colo/veterinária , Encephalitozoon/isolamento & purificação , Encefalitozoonose/veterinária , Lagartos , Animais , Doenças do Colo/diagnóstico , Doenças do Colo/microbiologia , Doenças do Colo/patologia , Diagnóstico Diferencial , Encefalitozoonose/diagnóstico , Encefalitozoonose/microbiologia , Encefalitozoonose/patologia , MasculinoRESUMO
Encephalitozoon cuniculi is an important microsporidian pathogen that is considered an emergent, zoonotic, and opportunistic. It infects both domestic and laboratory rabbits, generating severe chronic interstitial and granulomatous nephritis with fibrosis and granulomatous encephalitis. Encephalitozoonosis is diagnosed in paraffin-embedded sections by examining the spores in the host tissues. The spores are difficult to observe when the samples are stained with hematoxylin and eosin (H&E), particularly when there is an inflammatory reaction and tissue damage. The spores are easily mistaken for other microorganisms, such as fungi (yeasts), protozoa, and bacteria. In our study, we used kidney samples from E. cuniculi-positive rabbits and employed 14 recommended histologic stains for detecting microsporidia spores: alcian blue, calcofluor white, Giemsa, Gram, Grocott, H&E, Luna, Luxol fast blue, Masson trichrome, modified trichrome stain (MTS), periodic acid-Schiff reaction (PAS), Van Gieson, Warthin-Starry (WS), and Ziehl-Neelsen (ZN).We concluded that MTS and Gram stain, detected by light microscopy, and calcofluor white stain, detected by ultraviolet light microscopy, are the best stains for detecting spores of E. cuniculi in paraffin-embedded tissues from infected rabbits. These stains were superior to WS, ZN, Giemsa, and PAS for identifying spores without background "noise" or monochromatic interference. Also, they allow individual spores to be discerned in paraffin-embedded tissues. MTS allows observation of the polar tube, polaroplast, and posterior vacuole, the most distinctive parts of the spore.
Assuntos
Encephalitozoon cuniculi/isolamento & purificação , Encefalitozoonose/veterinária , Doenças dos Roedores/diagnóstico , Esporos Fúngicos/isolamento & purificação , Animais , Animais Domésticos , Encefalitozoonose/diagnóstico , Encefalitozoonose/parasitologia , Rim/parasitologia , Coelhos , Doenças dos Roedores/parasitologia , Coloração e Rotulagem/veterináriaRESUMO
Objectives Despite comprehensive diagnostics, the aetiology of meningoencephalitis (ME) in cats often remains undetermined. As a result of recently published surveys, Encephalitozoon cuniculi has gained growing importance in cats not only with ocular disorders, but also with central nervous system disease. Therefore, it was hypothesised that E cuniculi may be an underestimated pathogen in the development of feline non-suppurative and/or granulomatous ME. Methods As a first step, histopathological sections of the brain of cats with encephalopathy were retrospectively reviewed to identify cases of granulomatous ME. In a second step, an immunohistochemical screening for detection of E cuniculi was performed in cases with ME of unknown origin. Results In 59/89 (66.3%) cats with ME, an aetiologically relevant pathogen was detected. Forty-three of 89 (48.3%) cats had a diagnosis of feline infectious peritonitis. In 14/89 (15.7%) cats, protozoan cysts were identified and infection with Toxoplasma gondii was confirmed by immunohistochemistry (IHC) in all cases. In 2/89 (2.3%) cats with granulomatous ME, fungal organisms were identified. Thirty of 89 (33.7%) cats with ME of unknown origin that underwent IHC for the detection of E cuniculi remained negative. Conclusions and relevance The results of this study suggest that E cuniculi is unlikely to be directly associated with (non-suppurative and/or granulomatous) ME in cats in Austria.
Assuntos
Doenças do Gato/microbiologia , Encephalitozoon cuniculi , Encefalitozoonose/veterinária , Meningoencefalite/veterinária , Animais , Áustria , Doenças do Gato/diagnóstico , Doenças do Gato/patologia , Gatos , Encefalitozoonose/diagnóstico , Encefalitozoonose/patologia , Meningoencefalite/diagnóstico , Estudos Retrospectivos , ToxoplasmaRESUMO
Diagnosis of Encephalitozoon cuniculi infection in rabbits remains a major veterinary issue. ELISA or immunofluorescence assays are the current reference standards of serological tests. However, these conventional techniques suffer from a lack of accuracy for distinguishing active from past infections, as a positive serostatus is common in clinically normal rabbits. In this study, we assessed the diagnostic performance of Western blot (WB) to detect both anti-E. cuniculi immunoglobulin G (IgG) and immunoglobulin M (IgM) in comparison with ELISA and to address the intensity of the immune response through a quantitative approach. Positive WB results were highly correlated with the E. cuniculi-related diseased status (P < 0.0001). Although it was more labor intensive and less standardized, quantitative WB provided detailed comparable analysis regarding the humoral response and diagnostic performance similar to ELISA testing with statistically higher sensitivity (88.4 vs. 76.1% for IgG detection and 84.3 vs. 70.4% for IgM, P < 0.01). Several specific WB bands were shown to be significantly associated with concomitant clinical signs, like the one located at 50 kDa (OR = 8.2, [2.4-27.7], P = 0.0008) for IgG and (OR = 27.9, [4.2-187.9], P = 0.0006) for IgM. Therefore, the quantitative WB may have application in veterinary diagnostic laboratories to increase the accuracy of the clinical diagnosis of E. cuniculi infection. In addition, this tool may help to further understand the development and function of the humoral immune response to this infectious agent.
Assuntos
Western Blotting/veterinária , Encephalitozoon cuniculi/imunologia , Encefalitozoonose/veterinária , Coelhos/microbiologia , Animais , Western Blotting/métodos , Encephalitozoon cuniculi/isolamento & purificação , Encefalitozoonose/diagnóstico , Encefalitozoonose/microbiologia , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Técnica Direta de Fluorescência para Anticorpo/veterinária , Imunoglobulina G/sangue , Imunoglobulina M/sangue , MasculinoRESUMO
Detection of microsporidia at the species level is important for therapeutic purpose. The available techniques, modified trichrome (MT) staining cannot differentiate between species, while polymerase chain reaction (PCR) requires a reference laboratory and skilled technical staff. Immunoflourescence antibody (IFA) assay is another technique, which can differentiate among commonest species of microsporidia. However, there are very limited studies on its efficacy worldwide. Therefore, we aimed to evaluate IFA assay for the detection of microsporidia and differentiation among commonest species, Enterocytozoon bieneusi (E. bieneusi) and Encephalitozoon intestinalis infecting immunocompromised patients. Stool samples from 200 immunocompromised patients (19 with microsporidia and 181 without microsporidia using MT staining) were tested for species identification by PCR-RFLP and IFA assay. Sensitivity, specificity, diagnostic accuracy, and positive and negative predictive values were calculated as per standard formulae. Kappa statistics was used to assess the agreement between three tests. Of 200 immunocompromised patients, 21 and 20 patients had microsporidia using PCR and IFA assay, respectively. IFA assay and PCR identified E. bieneusi in all patients infected with microsporidia. Considering MT stain as gold standard, sensitivity and specificity of IFA assay was 100 and 99.4 %, respectively. Upon considering PCR as gold standard, sensitivity and specificity of IFA assay was 95.2 and 100 %, respectively. Diagnostic accuracy of IFA assay was 99.5 % along with its high test agreement with MT staining and PCR (K = 0.915, p = 0.049; K = 0.973, p = 0.027). IFA assay is highly sensitive and specific technique for detecting and identifying species of microsporidia among immunocompromised patients. E. bieneusi was the commonest species identified.
Assuntos
Encephalitozoon/imunologia , Encefalitozoonose/diagnóstico , Enterocytozoon/imunologia , Imunofluorescência/métodos , Enteropatias/diagnóstico , Microsporidiose/diagnóstico , Anticorpos Monoclonais , Encephalitozoon/genética , Encephalitozoon/isolamento & purificação , Encefalitozoonose/microbiologia , Enterocytozoon/genética , Enterocytozoon/isolamento & purificação , Fezes/microbiologia , Humanos , Hospedeiro Imunocomprometido , Enteropatias/microbiologia , Microsporidiose/microbiologia , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Sensibilidade e Especificidade , Coloração e RotulagemRESUMO
A European goldfinch (Carduelis carduelis), a canary (Serinus canaria), and a lovebird (Agapornis roseicollis) captive-bred at three different private aviaries in Spain were submitted for necropsy with a history of weakness and ruffled feathers, weight loss associated with glossitis, and respiratory disease, respectively. Microscopically, enterocytes in the jejunum and ileum contained colonies of gram- and Stamp-positive, oval to elliptical microorganisms within parasitophorous vacuoles in the apical cytoplasm. Nested PCR using MSP primers that target microsporidian RNA genes produced amplicons of expected size for Encephalitozoon species, and analysis of forward and reverse DNA sequences confirmed the presence of Encephalitozoon hellem in all cases. The main cause of death of all three birds consisted of concurrent infections. However, intestinal encephalitozoonosis may have contributed to exacerbated catabolism. Encephalitozoonosis (or microsporidiosis) has been rarely described in passerine birds.
Assuntos
Doenças das Aves/parasitologia , Encefalitozoonose/veterinária , Agapornis/parasitologia , Animais , Doenças das Aves/diagnóstico , Doenças das Aves/patologia , Canários/parasitologia , Encephalitozoon/fisiologia , Encefalitozoonose/diagnóstico , Encefalitozoonose/parasitologia , Encefalitozoonose/patologia , Feminino , Tentilhões/parasitologia , Intestinos/parasitologia , Intestinos/patologia , Masculino , EspanhaRESUMO
Five cases of microsporidioses among leukemic patients, 4 in myeloid-leukemic patients and 1 in a chronic lymphocytic leukemia, have been described until now. We report a case of microsporidiosis and the genomic identification of Encephalitozoon hellem in a patient with CD4(+) T-cell prolymphocytic leukemia.