RESUMO
Researchers often use trace element concentrations, including strontium-calcium ratios (Sr/Ca), to reconstruct paleodiets. While most commonly used as a proxy for meat consumption, a more appropriate application may be to differentiate frugivory from folivory. Sr/Ca ratios in animal tissue reflect the Sr/Ca ratios of the highest calcium components of that animal's diet. Because plants have much higher concentrations of calcium than meat, meat consumption signals are often overwhelmed by the variation in Sr/Ca ratios coming from different plant parts. This study uses faunal and plant data from Kibale National Park, a protected forest in southwestern Uganda home to numerous primate species (for example, common chimpanzees and baboons), to assess the reliability of Sr/Ca ratios to differentiate between primate dietary groups. We find that leaves consistently have higher strontium and calcium concentrations than fruits and that this is mirrored in higher Sr/Ca ratios in folivorous primates compared to frugivorous primates. Plant species differ widely in both their overall Sr/Ca ratios and the differences between their fruit and leaf Sr/Ca ratios, but this variation does not overwhelm the dietary signal separating frugivores and folivores. Furthermore, this research demonstrates that non-destructive and portable X-ray florescence (XRF) methods are an effective means of gathering Sr/Ca data from plant and faunal material, increasing the opportunities to apply such methods to fossil material in the future.
Assuntos
Cálcio , Estrôncio , Animais , Uganda , Estrôncio/análise , Cálcio/análise , Frutas , Primatas , Dieta , Parques Recreativos , Folhas de Planta/químicaRESUMO
The aim of the study was to determine the content and leachability of Sr in ashes obtained through combusting municipal waste in household furnaces. The waste had been collected as a mixed stream and as separate fractions (i.e. furniture, sponges, waste paper, PCV packaging, plastic-coated paper cartons, imitation leather, rubber, textiles and polystyrene). Using single-step chemical extractions, (HCl + HNO3, H2O, 0.01 M CaCl2, 0.1 M CH3COOH), we determined the total content of Sr (TC) and proportions of the following fractions: water-leachable, phytoavailable and easily soluble and bound to carbonates. We also analyzed the effect of reducing pH in the extraction solutions on St leachability from the study material. The study showed that Sr concentration in ash generated from the combustion of conventional fuels, alternative fuels and municipal waste ranged from 114 to 1006 mg/kg. The largest amounts of Sr were found in ash generated from the combustion of alternative fuels (coal pellets 488-1006 mg/kg), conventional fuels (hard coal 430-670 mg/kg) and mixed waste (237-825 mg/kg). The most mobile fraction of Sr (water-leachable) comprised from 1.3% to nearly 91% TC; the phytoavailable fraction and the ion-exchange and carbonate-bound fraction comprised 3-92% TC and 9-72% TC, respectively. We also found that the greatest pH reductions do not always entail the greatest amounts of extracted Sr. A much more significant factor in this respect is the mineral and chemical composition of primary materials, which can buffer changes in pH. The Risk Assessment Code (RAC) values pointed to a varied environmental risk and the highest RAC values (> 70) were found for coal pellets, wood pellets, straw, rubber and plastic containers for mixed oils.
Assuntos
Incineração , Estrôncio , Estrôncio/análise , Estrôncio/química , Concentração de Íons de Hidrogênio , Resíduos Sólidos/análise , Poluentes Químicos da Água/análiseRESUMO
This comprehensive review examines recent trends in phytoremediation strategies to address soil radionuclide contamination by cesium (Cs) and strontium (Sr). Radionuclide contamination, resulting from natural processes and nuclear-related activities such as accidents and the operation of nuclear facilities, poses significant risks to the environment and human health. Cs and Sr, prominent radionuclides involved in nuclear accidents, exhibit chemical properties that contribute to their toxicity, including easy uptake, high solubility, and long half-lives. Phytoremediation is emerging as a promising and environmentally friendly approach to mitigate radionuclide contamination by exploiting the ability of plants to extract toxic elements from soil and water. This review focuses specifically on the removal of 90Sr and 137Cs, addressing their health risks and environmental implications. Understanding the mechanisms governing plant uptake of radionuclides is critical and is influenced by factors such as plant species, soil texture, and physicochemical properties. Phytoremediation not only addresses immediate contamination challenges but also provides long-term benefits for ecosystem restoration and sustainable development. By improving soil health, biodiversity, and ecosystem resilience, phytoremediation is in line with global sustainability goals and environmental protection initiatives. This review aims to provide insights into effective strategies for mitigating environmental hazards associated with radionuclide contamination and to highlight the importance of phytoremediation in environmental remediation efforts.
Assuntos
Biodegradação Ambiental , Radioisótopos de Césio , Poluentes Radioativos do Solo , Radioisótopos de Estrôncio , Radioisótopos de Estrôncio/metabolismo , Radioisótopos de Estrôncio/análise , Poluentes Radioativos do Solo/metabolismo , Poluentes Radioativos do Solo/análise , Radioisótopos de Césio/metabolismo , Radioisótopos de Césio/análise , Solo/química , Plantas/metabolismo , Estrôncio/metabolismo , Estrôncio/análise , Césio/metabolismoRESUMO
BACKGROUND: An interlaboratory study was conducted at the U.S. Food and Drug Administration's (FDA) Northeast Food and Feed Laboratory (NFFL) and the Center for Food Safety and Applied Nutrition (CFSAN) with the purpose to expand FDA Elemental Analysis Manual (EAM) method 4.7 (Inductively Coupled Plasma-Mass Spectrometric Determination of Arsenic, Cadmium, Chromium, Lead, Mercury, and Other Elements in Food Using Microwave Assisted Digestion) to include new analytes. OBJECTIVE: The goal of the study was to demonstrate the performance of FDA EAM method 4.7 when analyzing new analytes cobalt (Co), strontium (Sr), thallium (Tl), tin (Sn), uranium (U), and vanadium (V). This analyte extension method validation of EAM 4.7 for six additional elements, Co, Sr, Tl, Sn, U, and V, followed all guidelines for a Level 2 or single-laboratory validation and met all acceptance criteria for analyte extensions as per the Guidelines for the Validation of Chemical Methods. METHOD: As per EAM 4.7, this study followed the procedures and used specified equipment operated under recommended conditions. The analyte extension method validation was performed in accordance with protocol and with no deviations. RESULTS: All quality control (QC) requirements for this analyte extension method validation of EAM 4.7 passed as evidenced by the analytical data. The results presented demonstrate accuracy, linearity, and precision by successful analyses of method blanks, matrix spikes, unfortified test samples, and reference materials. The data analyzed met each of the validation requirements for each analyte in all representative matrixes. CONCLUSIONS: The study showed that the new analytes performed satisfactorily using EAM 4.7 for total acidic extractable elemental analysis of food according to FDA's guidelines. HIGHLIGHTS: The method met or exceeded the performance criteria.
Assuntos
Cobalto , Estrôncio , Tálio , Estanho , Urânio , Cobalto/análise , Tálio/análise , Urânio/análise , Estanho/análise , Estrôncio/análise , Vanádio/análise , Análise de Alimentos/métodos , Espectrometria de Massas/métodos , Estados Unidos , United States Food and Drug AdministrationRESUMO
Ocean acidification could modify the bioavailability and chemical properties of trace elements in seawater, which could affect their incorporation into the calcareous structures of marine organisms. Fish otoliths, biomineralized ear stones made by aragonite, are suspended within the endolymph fluid of teleosts, indicating that the elemental incorporation of otoliths might also be susceptible to ocean acidification. In this study, we evaluated the combined effects of CO2-induced ocean acidification (pH 8.10, 7.70, and 7.30, corresponding to ocean acidification scenarios under the representative concentration pathway 8.5 model as projected by the Intergovernmental Panel on Climate Change) and water elemental concentrations of strontium (Sr) and barium (Ba; low, medium, and high) on elemental incorporation into otoliths of the flounder Paralichthys olivaceus at early life stages. Our results revealed that the elemental incorporation of Sr and Ba into otoliths was principally dependent on the corresponding water elemental concentrations rather than on ocean acidification. Moreover, the partition coefficients (DMe) of Sr and Ba may stabilize after dynamic equilibrium is reached as the water elemental concentration increases, but are not affected by ocean acidification. Therefore, the incorporation of Sr and Ba into otoliths of the flounder at early life stages may not serve as an effective indicator of ocean acidification. In other words, the findings suggest that ocean acidification does not impact the incorporation of Sr and Ba incorporation into otoliths when tracing the temperature or salinity experiences of the flounder. Our findings will provide new knowledge for understanding the potential ecological effects of ocean acidification on the recruitment dynamics of fish species.
Assuntos
Bário , Linguado , Membrana dos Otólitos , Água do Mar , Estrôncio , Animais , Estrôncio/análise , Membrana dos Otólitos/química , Membrana dos Otólitos/crescimento & desenvolvimento , Bário/análise , Água do Mar/química , Linguado/crescimento & desenvolvimento , Concentração de Íons de Hidrogênio , Dióxido de Carbono , Linguados/crescimento & desenvolvimento , Linguados/metabolismo , Mudança Climática , Acidificação dos OceanosRESUMO
Tongling is a significant non-ferrous metal mining city in China, which produces waste that negatively impacts the area's water environment. It is essential to comprehend the hydrochemical properties and formation processes of groundwater to safeguard and utilize it efficiently. We explored major ions, strontium, and its isotopes in water and river-bottom samples from the northern (i.e., A-A' section) and southern (i.e., B-B' section) areas. The hydrochemical facies show the mining activities have a greater impact on surface water than on groundwater. Groundwater hydrochemical formation results from several factors, with water-rock interaction and ion exchange being primary. Additionally, the dissolution of calcite, dolomite, and feldspar, oxidation of pyrite, and hydrolysis of carbonate minerals also impact the formation of groundwater chemistry. Our analysis of strontium and its isotopes indicates that carbonate dissolution primarily occurred in the recharge area; the runoff from the recharge to the discharge area results in the dissolution of certain silicate rocks; calcite dissolution sources account for > 70% contribution in both surface water and groundwater water-rock interactions, whereas silicate rock dissolution sources and dolomite dissolution sources account for < 30%. Due to changed order of dissolved carbonate and silicate minerals during groundwater flow, the distribution of strontium and its isotopes in the A-A' section is opposite to that in the B-B' section. The findings provide a basis for developing, utilizing, managing, and protecting groundwater resources, especially in similar mining areas.
Assuntos
Água Subterrânea , Magnésio , Poluentes Químicos da Água , Monitoramento Ambiental/métodos , Poluentes Químicos da Água/análise , Carbonato de Cálcio/análise , Água Subterrânea/química , Mineração , Isótopos de Estrôncio/análise , Minerais/análise , Estrôncio/análise , Carbonatos/análise , Isótopos/análise , Silicatos/análise , Água/análiseRESUMO
Coccolithophores are biogeochemically and ecologically important phytoplankton that produce a composite calcium carbonate-based exoskeleton - the coccosphere - comprised of individual platelets, known as coccoliths. Coccoliths are stunning examples of biomineralization; their formation featuring exceptional control over both biomineral chemistry and shape. Understanding how coccoliths are formed requires information about minor element distribution and chemical environment. Here, the first high-resolution 3D synchrotron X-ray fluorescence (XRF) mapping of a coccolith is presented, showing that the lopadoliths of Scyphosphaera apsteinii display stripes of different Sr concentration. The presence of Sr stripes is unaffected by elevated Sr in the culture medium, macro-nutrient concentration, and light intensity, indicating that the observed stripiness is an expression of the fundamental coccolith formation process in this species. Current Sr fractionation models, by contrast, predict an even Sr distribution and will have to be modified to account for this stripiness. Additionally, nano-XANES analyses show that Sr resides in a Ca site in the calcite lattice in both high and low Sr stripes, confirming a central assumption of current Sr fractionation models.
Assuntos
Estrôncio , Estrôncio/análise , Haptófitas/química , Carbonato de Cálcio/química , Espectrometria por Raios X/métodos , BiomineralizaçãoRESUMO
BACKGROUND AND PURPOSE: Biological monitoring of trace element horses is a well-known tool for investigating potential bioaccumulation in urbanized and industrialized geographical areas. Some biomaterials such as hoof are considered as an important indicator of environmental pollution. Hooves can store trace elements for a long time compared to blood and this represents a scientific key to long-term monitoring of exposure to environmental pollutants. In the present study, samples of equine hoof and blood were taken from an experimental group of horses living in an industrialized area of Sicily (Italy) and from a control group of horses housed in a non-industrialized area of Sicily to evaluate the bioaccumulation of different trace elements in different substrates. METHODS: Hoof and blood mineral concentration of Li- Lithium, Cu- Copper, Zn- Zinc, Sr- Strontium and, Pb- Lead was processed by means of Thermo Scientific iCAP-Q ICP-MS spectrometer. Student T-test was applied to evaluate the differences between groups and substrates. Correlation analysis between substrates and hematological parameters was performed. RESULTS: Statistical analysis showed a significant statistical difference for Li (p < 0.0001) and Pb (p < 0.0001) between blood and hoof in both groups. A statistically significant difference was observed for Li (p < 0.0001), Cu(p < 0.01), Zn (p < 0.0001) and Pb (p < 0.0001) between substrates in the experimental group. The statistical analysis showed a significant difference of blood Zn (p < 0.01) and hoof Pb (p < 0.01) concentration between groups. Our results revealed a statistically higher concentration of minerals in the blood substrate than in the hoof in both groups. Li (p < 0.0001) and Zn (p < 0.001) were positively correlated between control and experimental group in blood substrate. No correlations were found (P > .05) in hoof between groups for other elements. A positive correlation with WBC was observed in Cu concentration for hoof substrate (p < 0.02) in control group and for Zn in experimental group (p < 0.02). A positive correlation with HGB and HCT was observed for Cu hoof concentration (p < 0.02) in experimental group and PLT resulted positively correlated (p < 0.02) with Pb blood substrate in control group. CONCLUSIONS: Horses were not overexposed to these elements in the analyzed groups. The study highlights the role of a new bioaccumulation substrate such as the horse hoof identified as an analytical matrix for the monitoring of heavy metal concentrations in domestic animals.
Assuntos
Casco e Garras , Metais Pesados , Oligoelementos , Cavalos , Animais , Cobre/análise , Oligoelementos/análise , Zinco/análise , Lítio/análise , Chumbo/análise , Estrôncio/análise , Sicília , Monitoramento Ambiental/métodos , Metais Pesados/análise , Minerais/análiseRESUMO
Eels have fascinated biologists for centuries due to their amazing long-distance migrations between freshwater habitats and very distant ocean spawning areas. The migratory life histories of the Japanese eel, Anguilla japonica, in the waters of south China are not very clear despite its ecological importance, and the need for fishery regulation and management. In this study, strontium (Sr) and calcium (Ca) microchemical profiles of the otoliths of silver eels were measured by X-ray electron probe microanalysis based on data collected from different habitats (including freshwater and brackish habitats), in the large subtropical Pearl River. The corresponding habitat preference characteristics were further analysed using redundancy analysis (RDA). A total of 195 Japanese eels were collected over 6 years. The collected individuals ranged from 180 to 771 mm in total length and from 8 to 612 g in body weight. Two-dimensional pictures of the Sr:Ca concentrations in otoliths revealed that the A. japonica in the Pearl River are almost entirely river eels, spending the majority of their lives in fresh water without exposure to salt water, while the catadromous migration time has delayed about 1 month in the Pearl River estuary in the past 20 years. RDA analysis further indicated that juveniles and adults preferred water with high salinity and high tide levels. Youth preferred habitats with high river fractals. Our findings contribute to a growing body of evidence showing that the eels are extremely scarce currently and conservation measures against them are imminent, including the protection of brackish and freshwater areas where they live in south China.
Assuntos
Anguilla , Rios , Humanos , Animais , Anguilla/fisiologia , Migração Animal , Cálcio/análise , Ecossistema , Estrôncio/análise , China , Água/análiseRESUMO
Hydroxyapatite (HAp) phantoms have been proposed as an alternative to plaster of Paris (poP) phantoms for the calibration of x-ray fluorescence-based systems for thein vivoquantification of bone lead and strontium which employ a coherent normalization procedure. The chemical composition of the material becomes critical in the calculation, or omission, of the coherent correction factor (CCF) required in this normalization procedure. This study evaluated the long-term chemical stability of HAp phantoms. Phantoms were prepared and allowed to age for a two week period and over a seven year period in ambient conditions. The chemical composition of the phantoms was then assessed by powder x-ray diffraction. Two week old phantoms were found to be composed of HAp with only a small amount of contamination from CaHPO4·2H2O. Seven year old phantoms were found to have converted nearly completely to a carbonate-bearing apatite in the form of Ca10(PO4)6(CO3)0.75(OH)0.5indicating that the HAp phantom material likely reacts with carbon dioxide in air over time forming a carbonate-bearing apatite. The influence of this chemical conversion was assessed at the level of relevant cross-sections. Calibration under the assumption that the material is HAp when in fact it is a carbonate-bearing apatite would result in not more than a 0.2%-2% bias in the total mass attenuation coefficient within the photon energy range of 0-100 keV. Differential scattering cross-section for coherent scattering was found to differ between HAp and carbonate-bearing apatite by 0.9%-2% for both a 35.5 keV and 88.0 keVγ-ray. This variation in the differential scattering cross-section for coherent scattering may introduce a ca. 2% bias in the CCF used within the coherent normalization-based calibration procedure. Using HAp phantoms as calibrators thus requires acknowledgement of this conversion in chemical form and possible introduction of uncertainty into the calibration procedure.
Assuntos
Durapatita , Estrôncio , Estrôncio/análise , Calibragem , Espectrometria de Fluorescência , Espectrometria por Raios X/métodos , CarbonatosRESUMO
Exposure to metal mixtures may affect children's health but the conclusions are controversial. We aimed to investigate the associations of metal mixture exposure with children's physical and behavioral development. 15 metals were detected in the urine samples of 278 preschoolers aged 3-6 years from eastern China. Multiple linear models and restricted cubic splines were used to evaluate dose-response relationships between single metal and children's physical and behavioral development. The Bayesian Kernel Machine Regression (BKMR) models, the weighted quantile sum (WQS) models and Quantile G-Computation were applied to evaluate the joint effects of metal mixtures. The results showed that arsenic (As) was negatively associated with z score of height for age (HAZ) in individual-metal models [ß (95%CI): - 0.22 (-0.38, -0.06), P = 0.006]. Concerning children's behavioral development, multiple-metal models demonstrated a negative association with strontium (Sr) [ß (95%CI): - 0.82 (-1.38, -0.26), P = 0.004], and a positive association with tin (Sn) [ß (95%CI): 0.69 (0.16, 1.21), P = 0.010]. Notably, these associations remained significant or suggestive even after adjustments for multiple tests, sensitivity analyses, and application of different statistical models, including BKMR, WQS, and Quantile G-Computation. Furthermore, the study identified a negative joint effect of the metal mixture on HAZ, as demonstrated by BKMR and Quantile G-Computation models, with As playing an irreplaceable role in this observed impact. In summary, exposure to As appears to have adverse effects on HAZ, while exposure to Sn may hinder children's behavioral development. Conversely, exposure to Sr may have a protective effect on children's behavioral development. Additionally, the combined impact of metal mixtures is implicated in potentially impairing children's physical development, particularly in terms of HAZ.
Assuntos
Arsênio , Exposição Ambiental , Humanos , Criança , Pré-Escolar , Exposição Ambiental/efeitos adversos , Exposição Ambiental/análise , Monitoramento Biológico , Teorema de Bayes , Metais/toxicidade , Metais/análise , Arsênio/toxicidade , Arsênio/análise , Estrôncio/análise , ChinaRESUMO
A facile modification of a strontium-based MOF using oxalic acid was carried out to prepare MTSr-OX MOF, which was used as a potential substance for eliminating 152+154Eu radioisotopes. Various analytical techniques were used to characterize MTSr-OX-MOF. The prepared MOF had a rod-like structure with a BET surface area of 101.55 m2 g-1. Batch sorption experiments were used to investigate the sorption performance of MTSr-OX-MOF towards 152+154Eu radionuclides where different parameters like pH, contact time, initial 152+154Eu concentration, ionic strength, and temperature were scrutinized to determine the optimum conditions for 152+154Eu removal. MTSr-OX-MOF showed superior effectiveness in the elimination of 152+154Eu with a maximum sorption capacity of 234.72 mg g-1 at pH 3.5. Kinetics fitted with the pseudo-second-order model and the Langmuir model correctly described the sorption mechanism. The thermodynamic variables were carefully examined, demonstrating that the 152+154Eu sorption was endothermic as well as spontaneous. The MTSr-OX-MOF has been found to be a significantly more effective sorbent towards 152+154Eu than that of many other adsorbents. When applied to real active waste, MTSr-OX-MOF demonstrated excellent removal performance for a wide range of radionuclides. As a result, the MTSr-OX-MOF can be recognized as an attractive solution for the 152+154Eu purification from active waste.
Assuntos
Monitoramento de Radiação , Poluentes Químicos da Água , Estrôncio/análise , Adsorção , Radioisótopos , Termodinâmica , Cinética , Concentração de Íons de Hidrogênio , Poluentes Químicos da Água/químicaRESUMO
River sharks (Glyphis spp.) and some sawfishes (Pristidae) inhabit riverine environments, although their long-term habitat use patterns are poorly known. We investigated the diadromous movements of the northern river shark (Glyphis garricki), speartooth shark (Glyphis glyphis), narrow sawfish (Anoxypristis cuspidata), and largetooth sawfish (Pristis pristis) using in situ laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) on vertebrae to recover elemental ratios over each individual's lifetime. We also measured elemental ratios for the bull shark (Carcharhinus leucas) and a range of inshore and offshore stenohaline marine species to assist in interpretation of results. Barium (Ba) was found to be an effective indicator of freshwater use, whereas lithium (Li) and strontium (Sr) were effective indicators of marine water use. The relationships between Ba and Li and Ba and Sr were negatively correlated, whereas the relationship between Li and Sr was positively correlated. Both river shark species had elemental signatures indicative of prolonged use of upper-estuarine environments, whereas adults appear to mainly use lower-estuarine environments rather than marine environments. Decreases in Li:Ba and Sr:Ba at the end of the prenatal growth zone of P. pristis samples indicated that parturition likely occurs in fresh water. There was limited evidence of prolonged riverine habitat use for A. cuspidata. The results of this study support elemental-environment relationships observed in teleost otoliths and indicate that in situ LA-ICP-MS elemental characterization is applicable to a wide range of elasmobranch species as a discriminator for use and movement across salinity gradients. A greater understanding of processes that lead to element incorporation in vertebrae, and relative concentrations in vertebrae with respect to the ambient environment, will improve the applicability of elemental analysis to understand movements across the life history of elasmobranchs into the future.
Assuntos
Tubarões , Rajidae , Animais , Tubarões/metabolismo , Ecossistema , Água Doce/química , Rajidae/metabolismo , Estrôncio/análise , Coluna Vertebral/químicaRESUMO
A novel analytical solution of non-linear chromatography in case of parabolic isotherm for frontal analysis was obtained by combination of Cole-Hopf and Laplace transform. It was used for simulation of strontium capturing on chromatographic column with aim to improve quantitative determination of low-level 90Sr activities. From the experimentally determined breakthrough curves, the retention factor and the number of theoretical plates were calculated using the Glueckauf and Wenzel relations and by fitting the breakthrough curves for the linear isotherm using MatLab. These were used to simulate the breakthrough curves using a parabolic isotherm solution where the non-linear term of the isotherm was taken as a small negative deviation of the retention factor. On the base of theoretical prediction and experimental data, procedure for automated capturing of strontium on chromatographic column with specific dimension and off line "on-column" Cherenkov detection on commercial ultra low-level liquid scintillation counter was developed. It was shown that analytical solution for parabolic isotherm in comparison with solution for linear isotherm gives better prediction of mass of captured Sr on column filled with small amount of Sr resin and SuperLig®620 in case of elevated Sr concentration, even when non-linear effect is not obvious. The solution also makes it possible to predict the mass of resin required for strontium isolation at 100% yield under given conditions. Considering the limited dimensions of the column, and consequently small mass of the resin in them, it resulted in the low efficiency of the columns, which, however, did not affect the yield in real conditions of isolation. The results have shown that the yields achieved after isolation on SuperLig®620 from real samples are 100%. In addition, it is shown that captured 90Sr can be detected through 90Y ingrowth, on column filled with strontium specific resin, with Cherenkov detection efficiency of at least 50%. The efficiency may be enhanced to 60%, depending on parameters which can affect detection efficiency change (type of column, resin type, surrounding solution, etc.). The developed procedures enable quantitative determination of 90Sr in natural water samples with MDAC below 12 mBq l-1 and solid (soil and vegetation) samples with MDAC below 6 Bq kg-1 within 2-3 days. The proposed solution may easily be implemented in radiochemical laboratories where this type of analysis is routinely done within environmental monitoring or other purposes.
Assuntos
Cromatografia , Radioisótopos de Estrôncio , Radioisótopos de Estrôncio/análise , Cromatografia/métodos , Estrôncio/análise , Estrôncio/química , Monitoramento AmbientalRESUMO
Colombia faces the complex humanitarian challenges of locating approximately 100,000 missing persons and identifying thousands who are deceased. Identification is a difficult task in many cases, because the skeletonized bodies are deteriorated, missing person data are unavailable for comparison, and the provenance of the remains is often totally unknown. Isotopic analysis of human tissues (e.g., bone, hair, nails, and teeth) aid in the identification process of unknown individuals because they can provide valuable information on possible geographic origin. This project evaluated the isotopic variability of carbon (C), oxygen (O), strontium (Sr), and lead (Pb) in modern Colombian teeth according to city, department (a political designation similar to "state" in the US or Mexico), and one of four geographically determined regions of origin; and assessed its utility for human identification in Colombia. Isotopic data (O-C-Sr-Pb) were analyzed from modern Colombians originating from the cities of Bogotá, Cali, and Neiva (n = 95); and these data were compiled with published Colombian data of individuals mainly from the city of Medellín (n = 61). Results indicate a wide range and overlap of O-C-Sr-Pb isotopic distribution according to the defined categories. However, differences between coastal and lowland individuals are observed when using δ18 O values, and differences between mountainous regions are observed when using 87 Sr/86 Sr values. In addition, this study suggests that the usefulness of isotopic analysis for unidentified individuals in Colombia would be with assigning them geographically using the designations of North, Central Andes, Eastern Andes, or Southwest Andes versus making classifications at a city or department level.
Assuntos
Antropologia Forense , Estrôncio , Humanos , Colômbia , Estrôncio/análise , Oxigênio/análise , Carbono/análise , Chumbo/análise , Isótopos/análise , Isótopos de Estrôncio/análiseRESUMO
Objectives: Intra-tooth patterns of trace elements barium (Ba) and strontium (Sr) have been used to infer human and nonhuman primate nursing histories, including australopithecine and Neanderthal juveniles. Here we contrast the two elemental models in first molars (M1s) of four wild baboons and explore the assumptions that underlie each. Materials and Methods: Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) was employed to create comprehensive calcium-normalized barium and strontium (Ba/Ca, Sr/Ca) maps of M1 enamel and dentine at 35 micron resolution. Results: Postnatal Ba/Ca values were typically high, peaking ~0.5 years of age and then decreasing throughout M1 crown formation; all four individuals showed minimal Ba/Ca values between ~1.2-1.8 years, consistent with field reports of the cessation of suckling. Enamel Sr/Ca did not support patterns of previous LA-ICP-MS spot sampling as the enamel rarely showed discrete Sr/Ca secretory zonation. Increases in Sr/Ca appeared in coronal dentine beginning ~0.3 years, with varied peak value ages (~0.7-2.7 years) and no evidence of a predicted postweaning decline. Discussion: Inferences of baboon weaning ages from initial Ba/Ca minima are more congruent with behavioral observations than Sr/Ca maxima; this is consistent with studies of captive macaques of known weaning ages. Elemental variation is more apparent in the coronal dentine than the enamel of these baboons, which may relate to its more rapid mineralization and protection from the oral environment. Inferences of nursing histories from enamel Sr/Ca patterns alone should be reconsidered, and elevated values of Ba/Ca and Sr/Ca in teeth formed after weaning require further study.
Assuntos
Dente , Animais , Humanos , Desmame , Bário/análise , Dente/química , Estrôncio/análise , PapioRESUMO
INTRODUCTION: This study evaluated the effects of diabetes mellitus (DM) on the nanostructure of root canal dentin using high-resolution transmission electron microscopy (HRTEM) and inductively coupled plasma mass spectrometry (ICP-MS). METHODS: Twenty extracted human premolars from diabetic and nondiabetic patients (n = 10 in each group) were decoronated and sectioned horizontally into 40 2-mm-thick dentin discs, with each disc designated for a specific test. ICP-MS was used to determine the different elemental levels of copper, lithium, zinc, selenium, strontium, manganese, and magnesium in diabetic and nondiabetic specimens. HRTEM was used to analyze the shape and quantity of the apatite crystals in diabetic and nondiabetic dentin at the nanostructural level. Statistical analysis was performed using Kolmogorov-Smirnov and Student t test (P < .05). RESULTS: ICP-MS revealed significant differences in trace element concentrations between the diabetic and nondiabetic specimens (P < .05), with lower levels of magnesium, zinc, strontium, lithium, manganese, and selenium (P < .05), and higher levels of copper in diabetic specimens (P < .05). HRTEM revealed that diabetic dentin exhibited a less compact structure with smaller crystallites and significantly more crystals in the 2500 nm2 area (P < .05). CONCLUSION: Diabetic dentin exhibited smaller crystallites and altered elemental levels more than nondiabetic dentin, which could explain the higher root canal treatment failure rate in diabetic patients.
Assuntos
Diabetes Mellitus , Selênio , Oligoelementos , Humanos , Magnésio/análise , Magnésio/farmacologia , Cobre/análise , Cobre/farmacologia , Manganês/análise , Manganês/farmacologia , Selênio/análise , Selênio/farmacologia , Cavidade Pulpar , Lítio/análise , Lítio/farmacologia , Oligoelementos/análise , Oligoelementos/farmacologia , Zinco/análise , Zinco/farmacologia , Estrôncio/análise , Estrôncio/farmacologia , DentinaRESUMO
The geographical authentication in the agrifood industry has become a major issue to guarantee the quality of food products. Olive oil (OO) is particularly a complex matrix and establishing a reliable approach for linking OO samples to their origin is an analytical challenge. In this study, the isotopic composition of carbon, strontium and the concentrations of seventeen elements were determined in OOs from Tunisia, Southern France and the South Basque country. The preliminary results overlapped and showed that, taken individually, the isotopic and elemental approaches were not discriminant. A linear discriminant analysis applied to δ13C, 87Sr/86Sr and to the concentrations of 4 selected trace elements (Fe, Mn, V and Cr) allowed to classify, with high resolution, olive oils into 3 groups according to their provenance. The combination of the plant growing environment, the geological background, the mineral composition of the soil and the production process lead to a novel approach to deal with fraudulent practices in OO sector.
Assuntos
Oligoelementos , Azeite de Oliva , Isótopos de Carbono/análise , Oligoelementos/análise , Estrôncio/análise , França , Isótopos de Estrôncio/análise , Isótopos/análiseRESUMO
Understanding mobility and landscape use is important in reconstructing subsistence behavior, range, and group size, and it may contribute to our understanding of phenomena such as the dynamics of biological and cultural interactions between distinct populations of Upper Pleistocene humans. However, studies using traditional strontium isotope analysis are generally limited to identifying locations of childhood residence or nonlocal individuals and lack the sampling resolution to detect movement over short timescales. Here, using an optimized methodology, we present highly spatially resolved 87Sr/86Sr measurements made by laser ablation multicollector inductively coupled plasma mass spectrometry along the growth axis of the enamel of two marine isotope stage 5b, Middle Paleolithic Neanderthal teeth (Gruta da Oliveira), a Tardiglacial, Late Magdalenian human tooth (Galeria da Cisterna), and associated contemporaneous fauna from the Almonda karst system, Torres Novas, Portugal. Strontium isotope mapping of the region shows extreme variation in 87Sr/86Sr, with values ranging from 0.7080 to 0.7160 over a distance of c. 50 km, allowing short-distance (and arguably short-duration) movement to be detected. We find that the early Middle Paleolithic individuals roamed across a subsistence territory of approximately 600 km2, while the Late Magdalenian individual parsimoniously fits a pattern of limited, probably seasonal movement along the right bank of the 20-km-long Almonda River valley, between mouth and spring, exploiting a smaller territory of approximately 300 km2. We argue that the differences in territory size are due to an increase in population density during the Late Upper Paleolithic.
Assuntos
Hominidae , Terapia a Laser , Homem de Neandertal , Dente , Animais , Humanos , Portugal , Dente/química , Isótopos de Estrôncio/análise , Estrôncio/análiseRESUMO
OBJECTIVES: So far, no 87 Sr/86 Sr mobility studies have been done for Neolithic remains from Belgium and information on the Sr isotopic variability in the region is scarce. This study aims to explore mobility in a Final Neolithic population from the funerary cave 'Grotte de La Faucille', contribute to the understanding of the isotopic composition of bioavailable Sr in Belgium, assess evidence for male mobility using proteomic analysis, and explore possible places of origin for nonlocal individuals. MATERIALS AND METHODS: The 87 Sr/86 Sr isotope ratio of dental enamel from six adults and six juveniles was determined. Liquid chromatography mass spectrometry-based protein analysis was employed to identify individuals of male biological sex. 87 Sr/86 Sr of micromammal teeth, snail shells, and modern plants from three geological areas in Belgium were measured to establish isotopic signatures for bioavailable strontium. Nonlocality was assessed by comparing human 87 Sr/86 Sr isotope ratios to the 87 Sr/86 Sr range for bioavailable Sr. RESULTS: Four individuals yielded 87 Sr/86 Sr isotope ratios consistent with a nonlocal origin. No statistical differences were found between adults and juveniles. Three males were detected in the sample set, of which two show nonlocal 87 Sr/86 Sr values. DISCUSSION: This study provides evidence for mobility in Final Neolithic Belgium. The four nonlocal 87 Sr/86 Sr signatures correspond with the 87 Sr/86 Sr of bio-available Sr in Dutch South Limburg, the Black Forest in Southwest Germany, and regions of France, such as parts of the Paris Basin and the Vosges. The results support the ruling hypothesis of connections with Northern France, brought to light by archeological research.