RESUMO
Pseudomonas syringae is a gram-negative plant pathogen that infects plants such as tomato and poses a threat to global crop production. In this study, a novel lytic phage infecting P. syringae pv. tomato DC3000, named phage D6, was isolated and characterized from sediments in a karst cave. The latent period of phage D6 was found to be 60 min, with a burst size of 16 plaque-forming units per cell. Phage D6 was stable at temperatures between 4 and 40 °C but lost infectivity when heated to 70 °C. Its infectivity was unaffected at pH 6-10 but became inactivated at pH ≤ 5 or ≥ 12. The genome of phage D6 is a linear double-stranded DNA of 307,402 bp with a G + C content of 48.43%. There is a codon preference between phage D6 and its host, and the translation of phage D6 gene may not be entirely dependent on the tRNA library provided by the host. A total of 410 open reading frames (ORFs) and 14 tRNAs were predicted in its genome, with 92 ORFs encoding proteins with predicted functions. Phage D6 showed low genomic similarity to known phage genomes in the GenBank and Viral sequence databases. Genomic and phylogenetic analyses revealed that phage D6 is a novel phage. The tomato plants were first injected with phage D6, and subsequently with Pst DC3000, using the foliar spraying and root drenching inoculum approach. Results obtained after 14 days indicated that phage D6 inoculation decreased P. syringae-induced symptoms in tomato leaves and inhibited the pathogen's growth in the leaves. The amount of Pst DC3000 was reduced by 150- and 263-fold, respectively. In conclusion, the lytic phage D6 identified in this study belongs to a novel phage within the Caudoviricetes class and has potential for use in biological control of plant diseases.
Assuntos
Genoma Viral , Filogenia , Doenças das Plantas , Pseudomonas syringae , Solanum lycopersicum , Pseudomonas syringae/virologia , Pseudomonas syringae/genética , Pseudomonas syringae/patogenicidade , Genoma Viral/genética , Solanum lycopersicum/virologia , Solanum lycopersicum/microbiologia , Doenças das Plantas/microbiologia , Doenças das Plantas/virologia , Fagos de Pseudomonas/genética , Fagos de Pseudomonas/isolamento & purificação , Fagos de Pseudomonas/classificação , Composição de Bases , Fases de Leitura Aberta , Sequenciamento Completo do Genoma , DNA Viral/genéticaRESUMO
Pseudomonas phage MD8 is a temperate phage isolated from the freshwater lake Baikal. The organisation of the MD8 genome resembles the genomes of lambdoid bacteriophages. However, MD8 gene and protein sequences have little in common with classified representatives of lambda-like phages. Analysis of phage genomes revealed a group of other Pseudomonas phages related to phage MD8 and the genomic layout of MD8-like phages indicated extensive gene exchange involving even the most conservative proteins and leading to a high degree of genomic mosaicism. Multiple horizontal transfers and mosaicism of the genome of MD8, related phages and other λ-like phages raise questions about the principles of taxonomic classification of the representatives of this voluminous phage group. Comparison and analysis of various bioinformatic approaches applied to λ-like phage genomes demonstrated different efficiency and contradictory results in the estimation of genomic similarity and relatedness. However, we were able to make suggestions for the possible origin of the MD8 genome and the basic principles for the taxonomic classification of lambdoid phages. The group comprising 26 MD8-related phages was proposed to classify as two close genera belonging to a big family of λ-like phages.
Assuntos
Bacteriófago lambda , Genes Virais , Fagos de Pseudomonas , Bacteriófago lambda/classificação , Bacteriófago lambda/genética , Fagos de Pseudomonas/classificação , Fagos de Pseudomonas/genéticaRESUMO
In recent years, the use of bacteriophages (or 'phages') against multidrug-resistant (MDR) bacteria including Pseudomonas aeruginosa has drawn considerable attention, globally. In this work, we report the isolation and detailed characterization of a highly lytic Pseudomonasphage DRL-P1 isolated from wastewater. Under TEM, DRL-P1 appeared as a member of the phage family Myoviridae. DRL-P1 featured rapid adsorption (~ 5 min), short-latency (~ 30 min), and large burst size (~ 100 PFU per infected cell). DRL-P1 can withstand a wide temperature range (4 °C to 40 °C) and pH (5.0 to 10.0) conditions. The 66,243 bp DRL-P1 genome (MN564818) encodes at least 93 ORFs, of which 36 were functionally annotated based on homology with similar phage proteins available in the databases. Comparative analyses of related genomes suggest an independent evolutionary history and discrete taxonomic position of DRL-P1 within genus Pbunavirus. No toxin or antibiotic resistance genes was identified. DRL-P1 is tolerant to lyophilization and encapsulation techniques and retained lytic activity even after 18 months of storage. We also demonstrated decontaminating potentials of DRL-P1 in vitro, on an artificially contaminated cover-slip model. To the best of our knowledge, this is the first Pbunavirus to be reported from India. Our study suggests DRL-P1 as a potential candidate for various applications.
Assuntos
Myoviridae , Fagos de Pseudomonas , Pseudomonas aeruginosa/virologia , Águas Residuárias , DNA Viral , Farmacorresistência Bacteriana Múltipla/genética , Genoma Viral , Myoviridae/classificação , Myoviridae/isolamento & purificação , Myoviridae/fisiologia , Fagos de Pseudomonas/classificação , Fagos de Pseudomonas/isolamento & purificação , Fagos de Pseudomonas/fisiologia , Águas Residuárias/microbiologia , Águas Residuárias/virologiaRESUMO
The complete genome sequence of the virulent bacteriophage PMBT3, isolated on the proteolytic Pseudomonas grimontii strain MBTL2-21, showed no significant similarity to other known phage genome sequences, making this phage the first reported to infect a strain of P. grimontii. Electron microscopy revealed PMBT3 to be a member of the family Siphoviridae, with notably long and flexible whiskers. The linear, double-stranded genome of 87,196 bp has a mol% G+C content of 60.4 and contains 116 predicted protein-encoding genes. A putative tellurite resistance (terB) gene, originally reported to occur in the genome of a bacterium, was detected in the genome of phage PMBT3.
Assuntos
Pseudomonas/virologia , Animais , Bacteriólise , Composição de Bases , Sequência de Bases , DNA Viral/genética , Genoma Viral/genética , Especificidade de Hospedeiro , Leite/microbiologia , Filogenia , Fagos de Pseudomonas/classificação , Fagos de Pseudomonas/genética , Fagos de Pseudomonas/fisiologia , Fagos de Pseudomonas/ultraestrutura , Siphoviridae/classificação , Siphoviridae/genética , Siphoviridae/fisiologia , Siphoviridae/ultraestrutura , Proteínas Virais/genética , Vírion/ultraestruturaRESUMO
Isolating single phages using plaque assays is a laborious and time-consuming process. Whether single isolated phages are the most lyse-effective, the most abundant in viromes, or those with the highest ability to make plaques in solid media is not well known. With the increasing accessibility of high-throughput sequencing, metaviromics is often used to describe viruses in environmental samples. By extracting and sequencing metaviromes from organic waste with and without exposure to a host-of-interest, we show a host-related phage community's shift, as well as identify the most enriched phages. Moreover, we isolated plaque-forming single phages using the same virome-host matrix to observe how enrichments in liquid media correspond to the metaviromic data. In this study, we observed a significant shift (p = 0.015) of the 47 identified putative Pseudomonas phages with a minimum twofold change above zero in read abundance when adding a Pseudomonas syringae DC3000 host. Surprisingly, it appears that only two out of five plaque-forming phages from the same organic waste sample, targeting the Pseudomonas strain, were highly abundant in the metavirome, while the other three were almost absent despite host exposure. Lastly, our sequencing results highlight how long reads from Oxford Nanopore elevates the assembly quality of metaviromes, compared to short reads alone.
Assuntos
Metagenoma , Metagenômica , Fagos de Pseudomonas/fisiologia , Pseudomonas/virologia , Ensaio de Placa Viral , Viroma , Biologia Computacional , Especificidade de Hospedeiro , Metagenômica/métodos , Fagos de Pseudomonas/classificação , Ensaio de Placa Viral/métodosRESUMO
Antibiotic resistance causes around 700,000 deaths a year worldwide. Without immediate action, we are fast approaching a post-antibiotic era in which common infections can result in death. Pseudomonas aeruginosa is the leading cause of nosocomial infection and is also one of the three bacterial pathogens in the WHO list of priority bacteria for developing new antibiotics against. A viable alternative to antibiotics is to use phages, which are bacterial viruses. Yet, the isolation of phages that efficiently kill their target bacteria has proven difficult. Using a combination of phages and antibiotics might increase treatment efficacy and prevent the development of resistance against phages and/or antibiotics, as evidenced by previous studies. Here, in vitro populations of a Pseudomonas aeruginosa strain isolated from a burn patient were treated with a single phage, a mixture of two phages (used simultaneously and sequentially), and the combination of phages and antibiotics (at sub-minimum inhibitory concentration (MIC) and MIC levels). In addition, we tested the stability of these phages at different temperatures, pH values, and in two burn ointments. Our results show that the two-phages-one-antibiotic combination had the highest killing efficiency against the P. aeruginosa strain. The phages tested showed low stability at high temperatures, acidic pH values, and in the two ointments. This work provides additional support for the potential of using combinations of phage-antibiotic cocktails at sub-MIC levels for the treatment of multidrug-resistant P. aeruginosa infections.
Assuntos
Antibacterianos/uso terapêutico , Queimaduras/tratamento farmacológico , Infecções por Pseudomonas/terapia , Fagos de Pseudomonas/fisiologia , Pseudomonas aeruginosa/virologia , Queimaduras/microbiologia , Humanos , Testes de Sensibilidade Microbiana , Terapia por Fagos , Filogenia , Infecções por Pseudomonas/microbiologia , Fagos de Pseudomonas/classificação , Fagos de Pseudomonas/genética , Fagos de Pseudomonas/isolamento & purificação , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/fisiologia , Rios/virologia , Esgotos/virologiaRESUMO
Phytopathogenic pseudomonads are widespread in the world and cause a wide range of plant diseases. In this work, we describe the Pseudomonas phage Pf-10, which is a part of the biopesticide "Multiphage" used for bacterial diseases of agricultural crops caused by Pseudomonas syringae. The Pf-10 chromosome is a dsDNA molecule with two direct terminal repeats (DTRs). The phage genomic DNA is 39,424 bp long with a GC-content of 56.5%. The Pf-10 phage uses a packaging mechanism based on T7-like short DTRs, and the length of each terminal repeat is 257 bp. Electron microscopic analysis has shown that phage Pf-10 has the podovirus morphotype. Phage Pf-10 is highly stable at pH values from 5 to 10 and temperatures from 4 to 60 °C and has a lytic activity against Pseudomonas strains. Phage Pf-10 is characterized by fast adsorption rate (80% of virions attach to the host cells in 10 min), but has a relatively small number of progeny (37 ± 8.5 phage particles per infected cell). According to the phylogenetic analysis, phage Pf-10 can be classified as a new phage species belonging to the genus Pifdecavirus, subfamily Studiervirinae, family Autographiviridae, order Caudovirales.
Assuntos
Bacteriófagos/fisiologia , Agentes de Controle Biológico , Produtos Agrícolas , Doenças das Plantas/prevenção & controle , Fagos de Pseudomonas/fisiologia , Pseudomonas syringae/virologia , Bacteriófagos/classificação , Bacteriófagos/genética , Bacteriófagos/isolamento & purificação , Composição de Bases , Caudovirales/genética , Produtos Agrícolas/microbiologia , DNA Viral/genética , Genoma Viral , Especificidade de Hospedeiro , Filogenia , Doenças das Plantas/microbiologia , Podoviridae/genética , Fagos de Pseudomonas/classificação , Fagos de Pseudomonas/genética , Fagos de Pseudomonas/isolamento & purificação , Siphoviridae/genética , VírionRESUMO
Pseudomonas phages PaGz-1 and PaZq-1, two new phages infecting Pseudomonas aeruginosa, were isolated from fresh water in Guangdong province, China. The genomes of these two phages consist of 93,975 bp and 94,315 bp and contain 175 and 172 open reading frames (ORFs), respectively. The genome sequences of PaGz-1 and PaZq-1 share 95.8% identity with a query coverage of 94%, suggesting that these two phages belong to two different species. Based on results of nucleotide sequence alignment, gene annotation, and phylogenetic analysis, we propose PaGz-1 and PaZq-1 as representative isolates of two species in the genus Pakpunavirus within the family Myoviridae.
Assuntos
Genoma Viral , Myoviridae/genética , Fases de Leitura Aberta , Filogenia , Fagos de Pseudomonas/genética , Pseudomonas aeruginosa/virologia , Sequência de Bases , China , Água Doce/microbiologia , Ontologia Genética , Anotação de Sequência Molecular , Myoviridae/classificação , Myoviridae/isolamento & purificação , Fagos de Pseudomonas/classificação , Fagos de Pseudomonas/isolamento & purificação , Pseudomonas aeruginosa/isolamento & purificação , Alinhamento de Sequência , Sequenciamento Completo do GenomaRESUMO
Bacteriophage PaBG is a jumbo Myoviridae phage isolated from water of Lake Baikal. This phage has limited diffusion ability and thermal stability and infects a narrow range of Pseudomonas aeruginosa strains. Therefore, it is hardly suitable for phage therapy applications. However, the analysis of the genome of PaBG presents a number of insights into the evolutionary history of this phage and jumbo phages in general. We suggest that PaBG represents an ancient group distantly related to all known classified families of phages.
Assuntos
Fagos de Pseudomonas/isolamento & purificação , Pseudomonas aeruginosa/virologia , Genoma Viral , Especificidade de Hospedeiro , Filogenia , Fagos de Pseudomonas/classificação , Fagos de Pseudomonas/genética , Fagos de Pseudomonas/fisiologia , Pseudomonas aeruginosa/fisiologiaRESUMO
Bleeding canker of horse chestnut trees is a bacterial disease, caused by the bacterium Pseudomonas syringae pv. aesculi, estimated to be present in ~ 50% of UK horse chestnut trees. Currently, the disease has no cure and tree removal can be a common method of reducing inoculum and preventing spread. One potential method of control could be achieved using naturally occurring bacteriophages infective to the causative bacterium. Bacteriophages were isolated from symptomatic and asymptomatic horse chestnut trees in three locations in the South East of England. The phages were found to be belonging to both the Myoviridae and Podoviridae families by RAPD PCR and transmission electron microscopy. Experimental coevolution was carried out to understand the dynamics of bacterial resistance and phage infection and to determine whether new infective phage genotypes would emerge. The phages exhibited different coevolution patterns with their bacterial hosts across time. This approach could be used to generate novel phages for use in biocontrol cocktails in an effort to reduce the potential emergence of bacterial resistance.
Assuntos
Aesculus/microbiologia , Fagos de Pseudomonas/classificação , Pseudomonas syringae/virologia , Especificidade de Hospedeiro , Myoviridae/classificação , Doenças das Plantas/microbiologia , Folhas de Planta/microbiologia , Podoviridae/classificação , Fagos de Pseudomonas/isolamento & purificação , Pseudomonas syringae/patogenicidade , Reino UnidoRESUMO
The lytic cold-active phage HU1, a member of the family Podoviridae, infects Pseudomonas lactis and was first isolated from raw cow's milk. In this study, we used deep sequencing to determine and analyze the DNA genome sequence of HU1. We identified a 42,551-base-pair genome comprising double-stranded DNA, with 69 predicted open reading frames and a GC content of 56.4%. A whole-genome comparison did not identify HU1 as a member of any previously reported cluster of Pseudomonas phages. By contrast, HU1 was most similar to AF, which infects P. putida, with nucleotide sequence alignment coverage of 24%. These results suggest that HU1 is a novel Pseudomonas phage.
Assuntos
Leite/virologia , Fagos de Pseudomonas/genética , Sequenciamento Completo do Genoma/métodos , Animais , Tamanho do Genoma , Lisogenia , Fases de Leitura Aberta , Fagos de Pseudomonas/classificaçãoRESUMO
Bacteriophages encode an arsenal of proteins to lyse bacteria by breaking their surface structures, constituting a promising alternative to antibiotics. However, the selection and bioengineering of endolysins and other phage lytic proteins need to be assisted by a previous knowledge of their molecular characteristics. In this study, all putative lytic proteins encoded in Pseudomonas phages were in silico examined to describe their diversity, host association and molecular evolution. A total of 491 proteins were identified among 223 phages, including endolysins, holins, pinholins, spanins, lipases and peptidases. Protein families and combination of functional domains were characteristic of phages belonging to the same genus, and these tended to infect a single host species. Clustering and phylogenetic analysis showed a protein grouping associated with bacterial host, and some functional domains being specific. Interestingly, most putative lytic proteins from phages infecting P. fluorescens and P. putida had negative net charges, opposed to most endolysins. Phage lifestyle also had an impact on protein variability, with transglycosylases, glucosaminidases, holins and spanins from lysogenic phages clustering into monophyletic nodes, suggesting the effect of a different selection pressure as a result of the co-option of a new function in the lysogenized bacteria.
Assuntos
Biodiversidade , Evolução Molecular , Fagos de Pseudomonas/classificação , Fagos de Pseudomonas/genética , Bactérias/virologia , Simulação por Computador , Especificidade de Hospedeiro , Filogenia , Fagos de Pseudomonas/química , Proteínas Virais/análiseRESUMO
Two morphologically different bacteriophages were isolated from the river and soil samples from various locations of Maharashtra, India against the phytopathogen Pseudomonas sp. that was recently reported to cause a new bacterial blight of pomegranate. Both the phages belonged to the order Caudovirales representing the families Siphoviridae (vB_Psp.S_PRɸL2) and Myoviridae (vB_Psp.M_SSɸL8). The multiplicity of infection ranged from 0.01 to 0.1, phage adsorption rate from 39% to 66%, latent period from 10 to 20â¯min with a burst size of 24-85 phage particles per infected host cell. The genome size of phages PRɸL2 and SSɸL8 was approximately 25.403â¯kb and 29.877â¯kb respectively. Restriction digestion pattern of phage genomic DNA was carried out for phage PRɸL2, Eco RI resulted in two bands and Hind III resulted in three bands while for phage SSɸL8, both Eco RI and Hind III each resulted in three bands. SDS-PAGE protein profile showed six bands for PRɸL2 and nine bands for SSɸL8 of different proteins. Phages showed high pH stability over a range of 4-9, temperature stability over a range of 4-50⯰C and UV radiation showed a reduction up to 89.36% for PRɸL2 and 96% for SSɸL8. In short, the present research work discusses for the first time in-detailed characterization of phages of a phytopathogen Pseudomonas sp. from Maharashtra, India, which can be further efficiently used for biological control of the causative agent of a new bacterial blight disease of pomegranate.
Assuntos
Lythraceae/microbiologia , Doenças das Plantas/microbiologia , Fagos de Pseudomonas/classificação , Fagos de Pseudomonas/isolamento & purificação , Pseudomonas/virologia , Caudovirales/classificação , Caudovirales/genética , Caudovirales/isolamento & purificação , Caudovirales/ultraestrutura , DNA Viral/análise , Especificidade de Hospedeiro , Concentração de Íons de Hidrogênio , Índia , Viabilidade Microbiana , Myoviridae/classificação , Myoviridae/genética , Myoviridae/isolamento & purificação , Myoviridae/ultraestrutura , Fagos de Pseudomonas/genética , Fagos de Pseudomonas/ultraestrutura , Siphoviridae/classificação , Siphoviridae/genética , Siphoviridae/isolamento & purificação , Siphoviridae/ultraestrutura , Temperatura , Raios Ultravioleta/efeitos adversos , Proteínas Virais/análiseRESUMO
The complete genomic sequence of Pseudomonas fluorescens bacteriophage PFP1, isolated from sewage samples collected in Liaoning Province, China, were sequenced in this study and found to be 40,914 bp long. The PFP1 genome is composed of linear double-stranded DNA with 55.81% G+C content and 45 putative protein-coding genes, and no rRNA and tRNA genes. Comparative genomics and phylogenetic analysis revealed that the Pseudomonas fluorescens phage PFP1 is a new member of the genus T7virus. This information can be used to develop novel phage-based control strategies against Pseudomonas fluorescens.
Assuntos
Bacteriófagos/genética , Fagos de Pseudomonas/genética , Pseudomonas fluorescens/virologia , Bacteriófagos/classificação , Bacteriófagos/isolamento & purificação , Composição de Bases , Sequência de Bases , Genoma Viral , Dados de Sequência Molecular , Fases de Leitura Aberta , Filogenia , Fagos de Pseudomonas/classificação , Fagos de Pseudomonas/isolamento & purificação , Análise de Sequência de DNARESUMO
Despite the abundance, ubiquity and impact of environmental viruses, their inherent genomic plasticity and extreme diversity pose significant challenges for the examination of bacteriophages on Earth. Viral metagenomic studies have offered insight into broader aspects of phage ecology and repeatedly uncover genes to which we are currently unable to assign function. A combined effort of phage isolation and metagenomic survey of Chicago’s nearshore waters of Lake Michigan revealed the presence of Pbunaviruses, relatives of the Pseudomonas phage PB1. This prompted our expansive investigation of PB1-like phages. Genomic signatures of PB1-like phages and Pbunaviruses were identified, permitting the unambiguous distinction between the presence/absence of these phages in soils, freshwater and wastewater samples, as well as publicly available viral metagenomic datasets. This bioinformatic analysis led to the de novo assembly of nine novel PB1-like phage genomes from a metagenomic survey of samples collected from Lake Michigan. While this study finds that Pbunaviruses are abundant in various environments of Northern Illinois, genomic variation also exists to a considerable extent within individual communities.
Assuntos
Variação Genética , Genoma Viral , Fagos de Pseudomonas/classificação , Fagos de Pseudomonas/genética , Biologia Computacional , Microbiologia Ambiental , Metagenômica , Fagos de Pseudomonas/isolamento & purificaçãoRESUMO
Psychrotrophic gram-negative Pseudomonas spp. represent a serious problem in the dairy industry as they can cause spoilage of milk and dairy products. Bacteriophages have moved into focus as promising biocontrol agents for such food spoilage bacteria. The virulent Siphoviridae phage PMBT14 was isolated on a mutant variant of P. fluorescens DSM 50090 challenged with an unrelated virulent P. fluorescens DSM 50090 Podoviridae phage (i.e., mutant strain DSM 50090R). PMBT14 has a 47,820-bp dsDNA genome with 76 predicted open reading frames (ORFs). Its genome shows no significant sequence similarity to that of known phages, suggesting that PMBT14 represents a novel phage. Phage PMBT14 could be a promising biocontrol agent for P. fluorescens in milk or dairy foods.
Assuntos
Genoma Viral , Lisogenia/fisiologia , Fagos de Pseudomonas/genética , Pseudomonas fluorescens/virologia , Siphoviridae/genética , Proteínas Virais/genética , Agentes de Controle Biológico , Mapeamento Cromossômico , DNA/genética , DNA/metabolismo , DNA Viral/genética , DNA Viral/metabolismo , Laticínios/microbiologia , Microbiologia de Alimentos , Ontologia Genética , Tamanho do Genoma , Humanos , Anotação de Sequência Molecular , Fases de Leitura Aberta , Filogenia , Fagos de Pseudomonas/classificação , Fagos de Pseudomonas/patogenicidade , Fagos de Pseudomonas/ultraestrutura , Análise de Sequência de DNA , Siphoviridae/classificação , Siphoviridae/metabolismo , Proteínas Virais/metabolismoRESUMO
Phage O4 of Pseudomonas aeruginosa was previously visualized as a short-tailed virus using a transmission electron microscope. In this work, the O4 genome was characterized to be a linear dsDNA molecule comprising 50509 bp with 76 predicted genes located in five clusters. Mass spectrometry showed that the O4 virion contains 6 putative structural proteins, 2 putative enzymes, and 7 hypothetical proteins. By analyzing a Tn5G transposon mutation library, eight genes, wbpR, wbpV, wbpO, wbpT, wbpS, wbpL, galU, and wzy, were identified and confirmed responsible for the phage-resistant phenotype; all of them are related to the synthesis of O-specific antigen (OSA) of lipopolysaccharide (LPS), indicating that OSA is the receptor for the adsorption of phage O4. Comparative genomic analysis revealed that the phage O4 genome shares little similarity to any known podovirus, indicating that phage O4 is classifiable as a novel member of the Podoviridae family.
Assuntos
Genoma Viral , Lisogenia/fisiologia , Podoviridae/genética , Fagos de Pseudomonas/genética , Pseudomonas aeruginosa/virologia , Receptores Virais/metabolismo , Proteínas Virais/genética , DNA/genética , DNA/metabolismo , Elementos de DNA Transponíveis , DNA Viral/genética , DNA Viral/metabolismo , Ontologia Genética , Anotação de Sequência Molecular , Antígenos O/química , Antígenos O/metabolismo , Filogenia , Podoviridae/classificação , Podoviridae/metabolismo , Fagos de Pseudomonas/classificação , Fagos de Pseudomonas/metabolismo , Receptores Virais/química , Análise de Sequência de DNA , Proteínas Virais/metabolismo , Vírion/genética , Vírion/metabolismoRESUMO
The combined use of phage and antibiotics can show synergistic antimicrobial effects, so-called phage-antibiotic synergy (PAS). Here, we screened and examined PAS against Pseudomonas aeruginosa in vitro. Testing four different phages infecting P. aeruginosa, phage KPP22 classified within the family Myoviridae genus Pbunavirus showed PAS with the widest range of antibiotics, and showed PAS with anti-Pseudomonas drugs such as piperacillin and ceftazidime. Thus, evidence suggests that the combined use of phage and antibiotics is a promising therapeutic strategy against P. aeruginosa infections, with consideration needed regarding the optimal selection and adequate application timing of these phages and antibiotics.
Assuntos
Antibacterianos/farmacologia , Ceftazidima/farmacologia , Myoviridae/fisiologia , Piperacilina/farmacologia , Fagos de Pseudomonas/fisiologia , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/fisiologia , Humanos , Testes de Sensibilidade Microbiana , Myoviridae/classificação , Terapia por Fagos , Fagos de Pseudomonas/classificação , Fagos de Pseudomonas/genética , Pseudomonas aeruginosa/virologiaRESUMO
We isolated a Pseudomonas phage infecting Pseudomonas fluorescens SA1 separated from a soil sample collected in Sichuan Province, China. This phage, which we named PPSC2, has a genome that is composed of a 97,330-bp-long linear double-stranded DNA with 47.51% G+C content and 168 putative protein-coding genes. We identified 20 tRNA genes in the genome of PPSC2, and the tRNA GC content ranged from 44.2% to 58.4%. Phylogenetic and BLASTn analysis revealed that the Pseudomonas phage PPSC2 should be considered a new member of the family Myoviridae.
Assuntos
Genoma Viral , Fagos de Pseudomonas/genética , Microbiologia do Solo , Composição de Bases , China , DNA Viral/genética , Genômica , Myoviridae/genética , Fases de Leitura Aberta , Filogenia , Fagos de Pseudomonas/classificação , Fagos de Pseudomonas/isolamento & purificação , RNA de Transferência/genética , Análise de Sequência de DNARESUMO
In this study, we characterize three phages (SL1 SL2, and SL4), isolated from hospital sewage with lytic activity against clinical isolates of multi-drug resistant Pseudomonas aeruginosa (MDR-PA). The host spectrum ranged from 41% to 54%, with all three phages together covering 79% of all tested clinical isolates. Genome analysis revealed that SL1 (65,849 bp, 91 open reading frames ORFs) belongs to PB1-like viruses, SL2 (279,696 bp, 354 ORFs) to phiKZ-like viruses and SL4 (44,194 bp, 65 ORFs) to LUZ24-like viruses. Planktonic cells of four of five selected MDR-PA strains were suppressed by at least one phage with multiplicities of infection (MOIs) ranging from 1 to 10-6 for 16 h without apparent regrowth of bacterial populations. While SL2 was most potent in suppressing planktonic cultures the strongest anti-biofilm activity was observed with SL4. Phages were able to rescue bacteria-infected wax moth larvae (Galleria melonella) for 24 h, whereby highest survival rates (90%) were observed with SL1. Except for the biofilm experiments, the effect of a cocktail with all three phages was comparable to the action of the best phage alone; hence, there are no synergistic but also no antagonistic effects among phages. The use of a cocktail with these phages is therefore expedient for increasing host range and minimizing the development of phage resistance.