RESUMO
The increasing global food demand is threatening the sustainability of agrifood production systems. The intensification of agricultural practices, with inadequate use of pesticides and fertilizers, poses major challenges to the good functioning of agroecosystems and drastically degrades the soil quality. Nanotechnology is expected to optimize the current farming practices and mitigate some associated impacts. Layered double hydroxides (LDHs) are a class of nanomaterials with high potential for use in agricultural productions, mostly due to their sustained release of nutrients. Considering its novelty and lack of studies on the terrestrial ecosystem, it is essential to assess potential long-term harmful consequences to non-target organisms. Our study aimed to evaluate the effect of Zn-Al-NO3 LDH and Mg-Al-NO3 LDH ageing on the survival and reproduction of two soil invertebrate species Enchytraeus crypticus and Folsomia candida. We postulated that the toxicity of nanomaterials to soil invertebrates would change with time, such that the ageing of soil amendments would mediate their impacts on both species. Our results showed that the toxicity of LDHs was species-dependent, with Zn-Al-NO3 LDH being more toxic to E. crypticus, while Mg-Al-NO3 LDH affected more F. candida, especially in the last ageing period, where reproduction was the most sensitive biological parameter. The toxicity of both nanomaterials increased with ageing time, as shown by the decrease of the EC50 values over time. The influence of LDH dissolution and availability of Zn and Mg in the soil pore water was the main factor related to the toxicity, although we cannot rule out the influence of other structural constituents of LDHs (e.g., nitrates and aluminium). This study supports the importance of incorporating ageing in the ecotoxicity testing of nanomaterials, considering their slow release, as effects on soil organisms can change and lead to more severe impacts on the ecosystem functioning.
Assuntos
Fertilizantes , Oligoquetos , Poluentes do Solo , Solo , Animais , Fertilizantes/toxicidade , Poluentes do Solo/toxicidade , Solo/química , Oligoquetos/efeitos dos fármacos , Nanoestruturas/toxicidade , Reprodução/efeitos dos fármacos , Hidróxidos/toxicidade , Hidróxidos/química , Ecossistema , Invertebrados/efeitos dos fármacosRESUMO
Terrestrial snails are a significant issue in agricultural production worldwide. The use of nitrogen - phosphorus - potassium (NPK) based fertilizers played an important role in meeting the food demand throughout the world, so its effectiveness against land snails needs to be investigated. This study was conducted to evaluate toxic lethal effect of New-Fort®, an inorganic NPK based fertilizer, in the field for 3, 7 and 10 days and in the laboratory for 24, 48 and 72 h against Theba pisana snails. Also, the impact of its sub-lethal doses (1/10, 1/5, 1/4 and 1/2 of 48 h-LD50) on biochemical parameters were determined under laboratory conditions. The results showed that the snails percent reduction in the field were 21.4, 61.0 and 80.0 % after 10 days' application of quarter, half and one field rate and the values of LD50 in the laboratory were 4.94, 4.56 and 4.24 mg/g b.w at 24, 48 and 72 h, respectively. New-Fort® sub-lethal doses caused a significant inhibition in catalase, γ-glutamyl transferase and acetylcholinesterase activities. It also elicited a significant elevation in glutathione S-transferase activity post exposure to 1/10 and 1/5 of LD50, whereas an opposite effect was occurred after exposure to 1/4 and 1/2 of LD50. Lipid peroxidation level was reduced in snails treated with 1/10 and 1/5 of LD50, whereas it increased in 1/4 and 1/2 of LD50- treated snails. Moreover, a significant inhibition in alkaline phosphatase activity at all tested doses, with the exception of 1/2 of LD50 was observed. An increase in alanine aminotransferase and aspartate aminotransferase activities were occurred after all tested doses exposure. Our findings highlighted on how biochemical changes can be exploited to better understand the mechanisms underlying New-Fort® fertilizer toxicity against the land snail, T. pisana, as well as how to benefit from NPK fertilizers application in snail control.
Assuntos
Fertilizantes , Moluscocidas , Animais , Fertilizantes/toxicidade , Acetilcolinesterase , Dose Letal Mediana , Agricultura , Moluscocidas/toxicidade , CaramujosRESUMO
Nanoagrochemicals have the potential to revolutionize agriculture towards a precision farming system, able to reduce application rates and consequently their environmental footprint, while keeping efficacy. Several nanoagrochemicals (including nanopesticides (Npes) and nanofertilizers (Nfer)) are already commercialized but the environmental risk assessment of these advanced materials is often lacking. In the present study, we studied the commercial fertilizer WELGRO® Cu + Zn and assessed its ecotoxicity to the soil invertebrate species Enchytraeus crypticus (Oligochaeta), further comparing it to its individual active substances CuO and ZnO. To get a comprehensive picture of possible effects, we used four types of highly relevant tests in LUFA 2.2 soil: 1) avoidance behaviour (2 days), 2) reproduction (OECD standard, 28 d), 3) its extension (56 d), and 4) the full life cycle (FLC) (46 d) - this high level of hazard screening allows for increased interpretation. The results confirmed the nano-features of WELGRO® and a higher toxicity than the mixture of the individual components CuO + ZnO. E. crypticus avoided the soil spiked with WELGRO® and CuO + ZnO, this being the most sensitive endpoint - avoidance behaviour. Both WELGRO® and the active substances were little to non-toxic based on the OECD standard test. However, the toxicity dramatically increased in the tests focussing on longer-term sustainability measures, i.e., 56 days, ca. 170 for WELGRO®. This seems related to the nano-features of WELGRO®, e.g., slow release of ions from the nanoparticles throughout time. The FLCt results showed WELGRO® affected hatching and juveniles' survival, being these the most sensitive life stages. Hence, under actual real world field usage scenarios, i.e., based on the recommended application rates, nanoenabled WELGRO® can affect oligochaete populations like enchytraeids, both via the immediate avoidance behaviour and also via prolonged exposure periods.
Assuntos
Oligoquetos , Poluentes do Solo , Óxido de Zinco , Animais , Fertilizantes/toxicidade , Óxido de Zinco/farmacologia , Poluentes do Solo/análise , Reprodução , Solo/química , Zinco/farmacologiaRESUMO
Soil microplastic pollution can have negative effects on organisms, including plants, but the underlying mechanisms are not fully understood. We tested whether structural or chemical properties of a microplastic cause its effects on plant above- and belowground growth and whether these effects can be influenced by earthworms. We conducted a factorial experiment in a greenhouse with seven common Central European grassland species. Microplastic granules of the synthetic rubber ethylene propylene diene monomer (EPDM),1 a frequently used infill material of artificial turfs, and cork granules with a comparable size and shape to the EPDM granules were used to test for structural effects of granules in general. To test for chemical effects, EPDM-infused fertilizer was used, which should have contained any leached water-soluble chemical components of EPDM. Two Lumbricus terrestris individuals were added to half of the pots, to test whether these earthworms modify effects of EPDM on plant growth. EPDM granules had a clear negative effect on plant growth, but since cork granules had a negative effect of similar magnitude, with an average decrease in biomass of 37 % in presence of granules, this is likely due to the structural properties of granules (i.e., size and shape). For some belowground plant traits, EPDM had a stronger effect than cork, which shows that there must be other factors playing into the effects of EPDM on plant growth. The EPDM-infused fertilizer did not have any significant effect on plant growth by itself, but it had in interaction with other treatments. Earthworms had an overall positive effect on plant growth and mitigated most of the negative effects of EPDM. Our study shows that EPDM microplastic can have negative effects on plant growth, and that these might be more related to its structural than to its chemical properties.
Assuntos
Oligoquetos , Humanos , Animais , Microplásticos , Plásticos , Fertilizantes/toxicidade , Elastômeros , EtilenosRESUMO
A multitude of possible contaminants can be contained in bio-based fertilizers (BBFs) because of their complex matrix. The chemical characterization of BBFs is a challenging analytical task. Therefore, it is important for sustainable agricultural production to develop standard procedures to assess new bio-based fertilizers for possible hazards related to their application in order to guarantee their safety for soils organisms, plants and the environment. There is a huge number of ecotoxicological tests for aquatic and terrestrial organisms. They were developed for the evaluation of chemicals, pesticides and industrial wastes on aquatic systems and soil functioning. These tests can be useful for the assessment of BBFs. Ecotoxicological tests in comparison to chemical analysis have the advantage to capture the effects of all possible contaminants and metabolites available in the product. The bioavailability of toxic compounds and their interaction are recorded while the cause-and-effect-chain is not elucidated. Numerous ecotoxicological tests work with liquid media, capturing the effects of pollutants that can be mobilized. Hence, standardized procedures how to produce solvents from BBFs are mandatory. Moreover, tests using the original (solid) material are necessary in order to determine the toxicity of a given BBF in its application form and to cover the potential toxicity of non-soluble compounds. To date there are no rules how to determine the ecotoxicological potential of BBFs. A tiered approach of chemical analytical parameters in combination with a set of ecotoxicological tests and the measurement of sensitive soil indicators seem to be a promising experimental setup for the evaluation of BBFs. A decision tree for such an approach was developed. An extended ecotoxicological test strategy of BBFs is mandatory to identify the most promising raw materials and BBF processing technologies to end up with sustainable fertilizer products showing a high agronomic efficiency.
Assuntos
Poluentes Ambientais , Solo , Fertilizantes/toxicidade , Agricultura , Plantas , EcotoxicologiaRESUMO
The demand for food to feed the growing world population has been promoting the indiscriminate use of chemical fertilizers, which can be detrimental to the environment. In order to maintain high crop productivity without damaging the ecosystem, biofertilizers have emerged as alternative to reduce the use of chemical fertilizers. So, environmentally safer biofertilizer can replace the exploitation of more toxic chemical fertilizer. Here, the fly Drosophila melanogaster was used to study the potential toxicity of the biofertilizer Beifort®. Flies were exposed to high concentrations of Beifort® in the diet (1.8 mL/L, 9.0 mL/L and 18 mL/L), and morphological and behavioral endpoints of toxicity were analyzed (development from egg to adult age, flies longevity, climbing performance, memory and learning of an associative learning, larvae digestive tract damage and plasmid DNA break). Beifort® did not modify flies development, survival, digestive track cell damage, locomotor activity or memory. Beifort® did not induce DNA breakage in vitro and had no toxicity to the non-target D. melanogaster after in vivo exposure. Thus, in addition of promoting the sustainable use of agricultural wastes, the exploitation of Beifort® can contribute to decrease the use of chemical fertilizers.
Assuntos
Drosophila melanogaster , Ecossistema , Animais , Fertilizantes/toxicidade , Fertilizantes/análise , Agricultura , Produção AgrícolaRESUMO
The huge volumes of currently generated agricultural waste pose a challenge to the economy of the 21st century. One of the directions for their reuse may be as fertilizer. Spent mushroom substrate (SMS) could become an alternative to manure (M). A three-year field experiment was carried out, in which the purpose was to test and compare the effect of SMS alone, as well as in multiple variants with mineral fertilization, and in manure with a variety of soil quality indices-such as enzymatic activity, soil phytotoxicity, and greenhouse gas emissions, i.e., CO2. The use of SMS resulted in significant stimulation of respiratory and dehydrogenase activity. Inhibition of acid phosphatase and arylsulfatase activity via SMS was recorded. SMS showed varying effects on soil phytotoxicity, dependent on time. A positive effect was noted for the growth index (GI), while inhibition of root growth was observed in the first two years of the experiment. The effect of M on soil respiratory and dehydrogenase activity was significantly weaker compared to SMS. Therefore, M is a safer fertilizer as it does not cause a significant persistent increase in CO2 emissions. Changes in the phytotoxicity parameters of the soil fertilized with manure, however, showed a similar trend as in the soil fertilized with SMS.
Assuntos
Agaricales , Gases de Efeito Estufa , Fosfatase Ácida , Arilsulfatases , Dióxido de Carbono/toxicidade , Fertilizantes/toxicidade , Efeito Estufa , Esterco , Oxirredutases , SoloRESUMO
Stress exposures during vegetation are known to reduce the yield in crops, but the intensity and duration of stress is rather difficult to determine from the crop loss. Allelotoxins are released from plants into soil on exposure to stress factors. Soil allelotoxicity after vegetation was assumed to provide a diagnostic parameter that characterizes the total stress effect on crops. To verify the assumption, changes in soil allelotoxicity were studied after vegetation of three winter wheat cultivars fed with different amounts of nitrogen fertilizers. The cultivars Nemchinovskaya 85, Moskovskaya 39, and Lutescens 982/08 were found to differently respond to fertilizing. Nemchinovskaya 85 and Lutescens 982/08 showed lower stress responses at higher doses of nitrogen fertilizers, while their responses were appreciable when soil nitrogen was lacking. In the case of Moskovskaya 39, higher doses of nitrogen fertilizers caused an adverse reaction of increased allelotoxin release. Stepwise nitrogen fertilization was concluded to be necessary for preventing delayed plant development in the case of this cultivar. The results confirmed that soil allelotoxicity measured growing plants can serve as an indicator of stress caused by the growth conditions and use of various agricultural techniques.
Assuntos
Fertilizantes , Solo , Agricultura/métodos , Produtos Agrícolas , Fertilizantes/análise , Fertilizantes/toxicidade , Nitrogênio/análiseRESUMO
The increasing use of agrochemicals, alone and in combination, has been implicated as a potential causative factor in the decline of amphibians worldwide. Fertilizers and pesticides are frequently combined into single-use tank mixtures for agricultural applications to decrease costs while meeting the food demands of a growing human population. Limited data are available on the effects of increased nitrogen levels in nontarget species, such as amphibians, and therefore investigating alterations in the nitrogen cycle and its impacts on amphibians needs to be considered in best management practices going forward. The objective of the present study was to elucidate the impact of fertilizer (urea) and herbicide (atrazine and/or alachlor) tank mixtures on the hepatic metabolome of juvenile leopard frogs as well as to investigate alterations in oxidative stress by relating these changes to glutathione (GSH) levels. Herbicide exposure only moderately increased this parameter in amphibians, however, urea alone and in combination with either atrazine or alachlor statistically elevated GSH levels. Interestingly, urea also inhibited pesticide uptake: calculated bioconcentration factors were greatly decreased for atrazine and alachlor when urea was present in the exposure mixture. Metabolomic profiling identified fluxes in hepatic metabolites that are involved in GSH and carbohydrate metabolic processes as well as altered intermediates in the urea cycle. Ultimately, understanding the biological impacts of nitrogenous fertilizers alone and in combination with pesticide exposure will inform best management practices to conserve declining amphibian populations worldwide. Environ Toxicol Chem 2022;41:122-133. © 2021 SETAC.
Assuntos
Atrazina , Herbicidas , Praguicidas , Animais , Atrazina/metabolismo , Atrazina/toxicidade , Fertilizantes/toxicidade , Glutationa/metabolismo , Herbicidas/metabolismo , Herbicidas/toxicidade , Praguicidas/metabolismo , Rana pipiens , Ranidae , UreiaRESUMO
Whether maternal macro-environmental chemical fertilizer exposure has an association with the risk of preterm birth remains unclear. This study aimed to investigate the association between maternal exposure to chemical fertilizer during pregnancy and the risk of preterm birth. A total of 13,861 births, including 402 preterm birth from 313 villages in Pingding County, Shanxi Province, China, were analyzed to explore spatial variations of preterm birth risks at the village level. Spatial variations of preterm birth were visualized and tested with Disease Mapping, Moran's I and G* Statistic. The spatial zero-inflated negative binomial model was used to evaluate the incidence rate ratio (IRR) of preterm birth in different chemical fertilizer consumption levels at the village level. A population-based case-control study was conducted including 153 preterm births cases and 204 controls at the household level. The two-level logistic regression model was performed to estimate the association between risks of preterm birth and maternal exposure to chemical fertilizer after adjusting confounding factors. The findings indicated a remarkably clustering effect in relative risks of preterm birth and identified a high-risk clustering region of preterm birth from the south-central to the southwest and a low-risk clustering region in the northern Pingding county. The results of the spatial zero-inflated negative binomial model showed that the risk of preterm birth in the villages with chemical fertilizer consumption≥100 tons was 2.82 (95% CI: 1.50-5.57) times higher than those with <50 tons. Maternal exposure to chemical fertilizer ≥100 tons at village level was associated with an increased risk of preterm birth (aOR 2.14, 95%CI: 1.18-3.96), compared with exposure <50 tons after adjusting for confounding variables. The findings suggests that chemical fertilizers deserve more attention as a potential risk factor of preterm birth.
Assuntos
Nascimento Prematuro , Estudos de Casos e Controles , China/epidemiologia , Exposição Ambiental , Feminino , Fertilizantes/toxicidade , Humanos , Recém-Nascido , Exposição Materna , Gravidez , Nascimento Prematuro/induzido quimicamente , Nascimento Prematuro/epidemiologia , Fatores de RiscoRESUMO
Exposure to anthropogenic environmental chemical mixtures could be contributing to the decline in male reproductive health. This study used the biosolid treated pasture (BTP) sheep model to assess the effects of exposure to low-dose chemical mixtures. Maternal BTP exposure was associated with lower plasma testosterone concentrations, a greater proportion of Sertoli cell-only seminiferous tubules, and fewer gonocytes in the testes of neonatal offspring. Transcriptome analysis highlighted changes in testicular mTOR signalling, including lower expression of two mTOR complex components. Transcriptomic hierarchical analysis relative to the phenotypic severity demonstrated distinct differential responses to maternal BTP exposure during pregnancy. Transcriptome analysis between phenotypically normal and abnormal BTP lambs demonstrated separate responses within the cAMP and PI3K signalling pathways towards CREB. Together, the results provide a potential mechanistic explanation for adverse effects. Exposure could lower gonocyte numbers through mTOR mediated autophagy, but CREB mediated survival factors may act to increase germ cell survival.
Assuntos
Biossólidos , Poluentes Ambientais/toxicidade , Fertilizantes/toxicidade , Testículo/efeitos dos fármacos , Transcriptoma/efeitos dos fármacos , Animais , Animais Recém-Nascidos , AMP Cíclico , Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico , Feminino , Masculino , Troca Materno-Fetal , Fosfatidilinositol 3-Quinases , Gravidez , Ovinos , Transdução de Sinais , Serina-Treonina Quinases TOR , Testículo/metabolismo , Testículo/patologia , Testosterona/sangueRESUMO
Juvenile African Catfish (also known as Sharptooth Catfish) Clarias gariepinus were exposed to 2.26, 4.52, and 11.30 mg/L NPK (15-15-15) fertilizer for 21 d followed by 7 d of recovery to assess the genotoxic effects of the fertilizer in erythrocytes. Biomarkers of oxidative stress were evaluated in the liver and gill tissues. The fertilizer induced micronuclei formation with maximum effects on day 7 in erythrocytes of individuals that were exposed to 4.52 and 11.30 mg/L NPK, and on day 14 in individuals exposed to 2.26 mg/L of the same fertilizer. The lipid peroxidation, glutathione reductase, and reduced glutathione values in the exposed fish increased, while the values of catalase, superoxide dismutase, and glutathione peroxidase decreased. There were mixed trends in the recovery patterns after the 7-d withdrawal from the fertilizer. Careful use of the fertilizer in the field is recommended to avoid toxicological effects on nontarget organisms.
Assuntos
Peixes-Gato/metabolismo , Fertilizantes/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Animais , Cálcio/química , Peixes-Gato/genética , Eritrócitos/efeitos dos fármacos , Eritrócitos/enzimologia , Brânquias/efeitos dos fármacos , Fígado/efeitos dos fármacos , Micronúcleos com Defeito Cromossômico , Nitrogênio/química , Fósforo/química , Poluentes Químicos da Água/toxicidadeRESUMO
Poultry litter is used as a fertilizer due to the high concentration of nutrients. However, this material receives application of medicines, whether they are used to treat animals or the litter itself. Thus, the objective was to evaluate if the application in soils of poultry litter doses treated with two insecticides (cypermethrin and cinnamon oil) used to control of the Alphitobius diaperinus, affect the reproduction of Enchytraeus crypticus, and the insecticides effects over time, trough of ecotoxicological tests using soils with different textural classes an Entisol and an Oxisol. In the Oxisol the application of poultry litter stimulated the E. crypticus reproduction regardless of the insecticide and the collection time. In Entisol the poultry litter showed a toxicity at 10 t ha-1 when treated with cypermethrin and at 30 t ha-1 with cinnamon oil. Entisol results show that cypermethrin affects non-target edaphic organisms soon after spraying, but over time has reduced toxicity unlike cinnamon oil.
Assuntos
Cinnamomum zeylanicum , Fertilizantes/toxicidade , Inseticidas/toxicidade , Óleos Voláteis/toxicidade , Oligoquetos/efeitos dos fármacos , Piretrinas/toxicidade , Poluentes do Solo/toxicidade , Animais , Galinhas , Esterco , Reprodução/efeitos dos fármacosRESUMO
PURPOSE: The aim of this study was to prepare zeolite/iron (III) oxide nanocomposites (zeolite/Fe2O3-NCs) as a smart fertilizer to improve crop yield and soil productivity. METHODS: Zeolite/Fe2O3-NCs were successfully produced by loading of Fe2O3-NPs onto the zeolite surface using a quick green precipitation method. The production of zeolite/Fe2O3 nanocomposites was performed under a mild condition using environmentally friendly raw materials as a new green chemistry method. The product was characterized using several techniques such as near and far Fourier-transform infrared spectroscopy (FT-IR), powder X-ray diffraction (PXRD), energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). RESULTS: The results confirmed the formation of Fe2O3-NPs with mean particle sizes of 1.45, 2.19, and 2.20 nm on the surface of the zeolite per amount of 4, 7 and 12 wt% Fe2O3-NPs, respectively. Such results indicated that the size of the Fe2O3-NPs did not significantly change when Fe amounts increased from 7 to 12 wt% for the zeolite/Fe2O3-NCs. In terms of medical applications, in vitro cell studies demonstrated that zeolites and zeolite/Fe2O3-NCs were generally non-toxic to human fibroblast cells and significantly pernicious to human malignant melanoma cells. From MTS cytotoxicity assays, the concentration of Fe2O3 within the zeolite/Fe2O3-NCs that was effective at inhibiting the growth of malignant melanoma cells by 50% (the IC50 value) was ~14.9 wt%. The three types of nanocomposites were further tested as an iron smart nanofertilizer for the slow-release of iron ions. CONCLUSION: Advantages of this project include the production of non-toxic nanocomposites as a smart fertilizer to develop crops while the reaction involves the use of commercial and natural materials as low-cost raw materials with low energy usage due to a mild reaction condition, as well as the use of an environmentally friendly solvent (water) with no toxic residues.
Assuntos
Compostos Férricos/química , Fertilizantes , Nanocompostos/química , Zeolitas/química , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Fertilizantes/toxicidade , Fibroblastos/efeitos dos fármacos , Química Verde , Humanos , Ferro/farmacocinética , Melanoma/tratamento farmacológico , Melanoma/patologia , Microscopia Eletrônica de Transmissão , Nanocompostos/toxicidade , Tamanho da Partícula , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios XRESUMO
The QuEChERS (quick, easy, cheap, effective, rugged, and safe) ultrasound-assisted method was successfully used to determine the presence of contaminants of emerging concern (CECs) in both the growing medium (i.e. soil) and vegetable samples (i.e. potato, onion, celery, parsnip and carrot) cultivated in household gardens under field conditions impacted by the application of livestock manure mixed with human waste. CECs with a broad range of physico-chemical properties including pharmaceuticals, plasticizers, herbicides, personal care products, and biocides were investigated. Among all studied CECs, diclofenac (DCL), carbamazepine, bisphenol A (BPA) and estrogenic hormones (estrone (E1), 17α-ethinyl estradiol (EE2)) were detected and quantified in vegetable samples with a concentration range from 0.114 to 13.3 ng/g fresh weight (f.w.), while BPA, E1, and EE2 were detected in soil samples with concentration ranges from 0.526 to 0.830 ng/g f.w., 0.121-0.199 ng/g f.w. and 0.118-0.333 ng/g f.w., respectively. DCL was only quantified above the limit of quantification in one soil sample (0.151 ng/g f.w.). The human health risk was estimated using the threshold of toxicological concern approach and a standard diet for two segments of the population (i.e. adult and child). The consumption of investigated vegetables contaminated with CECs poses no risk for the Serbian population.
Assuntos
Agricultura , Saúde Ambiental , Fezes , Fertilizantes , Poluentes Químicos da Água , Criança , Etinilestradiol , Fertilizantes/toxicidade , Humanos , Medição de Risco , Solo , Águas ResiduáriasRESUMO
BACKGROUND: N fixation is one of the most important microbially driven ecosystem processes on Earth, allowing N to enter the soil from the atmosphere, and regulating plant productivity. A question that remains to be answered is whether such a fundamental process would still be that important in an over-fertilized world, as the long-term effects of fertilization on N fixation and associated diazotrophic communities remain to be tested. Here, we used a 35-year fertilization experiment, and investigated the changes in N fixation rates and the diazotrophic community in response to long-term inorganic and organic fertilization. RESULTS: It was found that N fixation was drastically reduced (dropped by 50%) after almost four decades of fertilization. Our results further indicated that functionality losses were associated with reductions in the relative abundance of keystone and phylogenetically clustered N fixers such as Geobacter spp. CONCLUSIONS: Our work suggests that long-term fertilization might have selected against N fixation and specific groups of N fixers. Our study provides solid evidence that N fixation and certain groups of diazotrophic taxa will be largely suppressed in a more and more fertilized world, with implications for soil biodiversity and ecosystem functions.
Assuntos
Agricultura/métodos , Fertilizantes , Fixação de Nitrogênio , Nitrogênio/metabolismo , Microbiologia do Solo , Biodiversidade , Ecossistema , Fertilizantes/microbiologia , Fertilizantes/toxicidade , FilogeniaRESUMO
Increasing agricultural production requires the application of more chemical inputs during the growth of different crops. This study aims at assessing the energy indicators and environmental impacts during the initial 7-year growth of orange orchards. Through the life cycle assessment (LCA) technique, the hotspots in 11 environmental indicators of the orange orchard growth were determined for different years. The system boundaries were considered to be the orange orchard gates, and the functional units were 1 kg of orange and 1-h orange orchard. The energy input of nitrogen fertilizer, diesel, and human labor has the biggest share in the total energy inputs. The total average energy input and energy output (in 7 years) were calculated as 62,917.027 MJ/ha and 47,618.17 MJ/ha, respectively. The results showed that the energy indices (energy efficiency and net energy) were increasing from year first to seventh and also the share of renewable energy increased. According to the results, with respect to the highest share for production of 1 kg orange in different criteria, nitrogen fertilizer was the main contributor to abiotic depletion and human toxicity, orchard field emissions and nitrogen fertilizer had the highest shares in global warming and photochemical oxidation, fossil fuels and nitrogen fertilizer were the highest contributors to ozone layer depletion, whereas ecotoxicity was mainly affected by chemical fertilizers and orchard surface emissions. Finally, the main contributor for acidification and eutrophication was surface emissions. In the study of environmental impacts from the first to the seventh year, it should be noted that if the functional unit is considered mass based (1 kg orange), due to the unproductive of the trees in the first to third years (low fruit production), the environmental effects are high and then it decreases after the third year due to increasing the yield of the product. Generally, with consideration of the tree growth period, the useful data of energy and environmental impacts for production horticultural products can be provided so that we can avoid multiple interpretations of results associated to reporting annual energy and environmental impact variations.
Assuntos
Citrus sinensis/crescimento & desenvolvimento , Meio Ambiente , Nitrogênio/química , Agricultura/métodos , Eutrofização , Fertilizantes/toxicidade , Combustíveis Fósseis , Aquecimento Global , Humanos , Irã (Geográfico)RESUMO
The adaptation of crops to acid soils is needed for the maintenance of food security in a sustainable way, as decreasing fertilizers use and mechanical interventions in the soil would favor the reduction of agricultural practices' environmental impact. Phosphate deficiency and the presence of reactive aluminum affect vital processes to the plant in this soil, mostly water and nutrient absorption. From this, the understanding of the molecular response to these stresses can foster strategies for genetic improvement, so the aim was to broadly analyze the transcriptional variations in Poupulus spp. in response to these abiotic stresses, as a plant model for woody crops. A co-expression network was constructed among 3,180 genes differentially expressed in aluminum-stressed plants with 34,988 connections. Of this total, 344 genes presented two-fold transcriptional variation and the group of genes associated with those regulated after 246 hours of stress had higher number of connections per gene, with some already characterized genes related to this stress as main hubs. Another co-expression network was made up of 8,380 connections between 550 genes regulated by aluminum stress and phosphate deficiency, in which 380 genes had similar profile in both stresses and only eight with transcriptional variation higher than 20%. All the transcriptomic data are presented here with functional enrichment and homology comparisons with already characterized genes in another species that are related to the explored stresses, in order to provide a broad analysis of the co-opted responses for both the stresses as well as some specificity. This approach improves our understanding regarding the plants adaptation to acid soils and may contribute to strategies of crop genetic improvement for this condition that is widely present in regions of high agricultural activity.
Assuntos
Alumínio/toxicidade , Fosfatos/metabolismo , Populus/genética , Estresse Fisiológico/genética , Adaptação Fisiológica/genética , Produtos Agrícolas , Fertilizantes/toxicidade , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Redes Reguladoras de Genes/efeitos dos fármacos , Humanos , Populus/efeitos dos fármacos , Populus/metabolismo , Solo/química , Inanição/genética , Transcriptoma/efeitos dos fármacos , Transcriptoma/genéticaRESUMO
Phthalate acid esters (PAEs) are the most frequently utilized synthetic chemical compounds worldwide. They are typical emergent contaminants and are currently attracting considerable concern due to their risks to plants, animals, and public health. Determining the vital environmental factors that affect the toxicity of target pollutants in soil is important for vegetable production and the maintenance and control of soil productivity. We investigated the influence of di-n-butyl phthalate (DBP) and bis(2-ethylhexyl) phthalate (DEHP) under different soil conditions on physiological changes in water spinach (Ipomoea aquatic Forsk.) seedlings and the rhizosphere soil microbial community. Supported by our former experiments in which we determined the representative concentrations that caused the most pronounced toxic effects, three experimental concentrations were studied including control soils without PAEs and spiked soils with either 20 mg DBP or DEHP kg-1 soil. The soil at all the three PAE concentrations was then adjusted to test two soil pH values, three levels of soil organic matter (SOM) content, and three levels of soil moisture content; thus, we completed 12 treatments or conditions simulating different soil environment conditions in greenhouses. After 30 days of cultivation, we analyzed the toxicity effects of two target PAEs on plant growth and physiological factors, and on soil microbial community characteristics. The toxicity of soil DBP and DEHP to the physiology of water spinach was found to be most affected by the soil pH value, then by SOM content, and least of all by soil moisture. The results of the 454 high-throughput sequencing analysis of the soil microbial community indicated that the toxicity of target PAEs to soil microorganisms was most affected by SOM content and then by soil moisture, and no clear relationship was found with soil pH. Under different soil conditions, declines in leaf biomass, chlorophyll a content, and carotenoid content-as well as increases in free amino acid (FAA) content, superoxide anion free radical activity, and hydroxyl radical activity-occurred in response to DBP or DEHP. Heavy use of chemical fertilizer, organic fertilizer, and high humidity led to the special environmental conditions of greenhouse soil, constituting the main conditions considered in this study. The results indicate that under the special highly intensive production systems of greenhouses, soil conditions may directly influence the effects of pollutant phytotoxicity and may thus endanger the yield, nutrient content, and food safety of vegetables. The combined studies of the impacts on plants and rhizosphere microorganisms give a more detailed picture of the toxic effects of the pollutants under different soil conditions.
Assuntos
Dibutilftalato/toxicidade , Ipomoea/efeitos dos fármacos , Microbiota , Plastificantes/toxicidade , Plântula/efeitos dos fármacos , Poluentes do Solo/toxicidade , Verduras/efeitos dos fármacos , Produção Agrícola , Fertilizantes/toxicidade , Microbiologia do SoloRESUMO
Chicken litter application on land as an organic fertilizer is the cheapest and most environmentally safe method of disposing of the volume generated from the rapidly expanding poultry industry worldwide. However, little is known about the safety of chicken litter for land application and general release into the environment. Bridging this knowledge gap is crucial for maximizing the benefits of chicken litter as an organic fertilizer and mitigating negative impacts on human and environmental health. The key safety concerns of chicken litter are its contamination with pathogens, including bacteria, fungi, helminthes, parasitic protozoa, and viruses; antibiotics and antibiotic-resistant genes; growth hormones such as egg and meat boosters; heavy metals; and pesticides. Despite the paucity of literature about chicken litter safety for land application, the existing information was scattered and disjointed in various sources, thus making them not easily accessible and difficult to interpret. We consolidated scattered pieces of information about known contaminants found in chicken litter that are of potential risk to human, animal, and environmental health and how they are spread. This review tested the hypothesis that in its current form, chicken litter does not meet the minimum standards for application as organic fertilizer. The review entails a meta-analysis of technical reports, conference proceedings, peer-reviewed journal articles, and internet texts. Our findings indicate that direct land application of chicken litter could be harming animal, human, and environmental health. For example, counts of pathogenic strains of Eschericiacoli (105-1010 CFU g-1) and Coliform bacteria (106-108 CFU g-1) exceeded the maximum permissible limits (MPLs) for land application. In Australia, 100% of broiler litter tested was contaminated with Actinobacillus and re-used broiler litter was more contaminated with Salmonella than non-re-used broiler litter. Similarly, in the US, all (100%) broiler litter was contaminated with Eschericiacoli containing genes resistant to over seven antibiotics, particularly amoxicillin, ceftiofur, tetracycline, and sulfonamide. Chicken litter is also contaminated with a vast array of antibiotics and heavy metals. There are no standards set specifically for chicken litter for most of its known contaminants. Even where standards exist for related products such as compost, there is wide variation across countries and bodies mandated to set standards for safe disposal of organic wastes. More rigorous studies are needed to ascertain the level of contamination in chicken litter from both broilers and layers, especially in developing countries where there is hardly any data; set standards for all the contaminants; and standardize these standards across all agencies, for safe disposal of chicken litter on land.