RESUMO
Highly selective and fast liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for simultaneous determination of tadalafil (TDL) and finasteride (FNS) in human plasma. The method was successfully applied for analysis of TDL and FNS samples in clinical study. The method was validated as per USFDA (United States Food and Drug Administration), EMA (European Medicines Agency), and ANVISA (Agência Nacional de Vigilância Sanitária-Brazil) bio analytical method validation guidelines. Glyburide (GLB) was used as common internal standard (ISTD) for both analytes. The selected multiple reaction monitoring (MRM) transitions for mass spectrometric analysis were m/z 390.2/268.2, m/z 373.3/305.4 and m/z 494.2/369.1 for TDL, FNS and ISTD respectively. The extraction of analytes and ISTD was accomplished by a simple solid phase extraction (SPE) procedure. Rapid analysis time was achieved on Zorbax Eclipse C18 column (50â¯×â¯4.6â¯mm, 5⯵m). The calibration ranges for TDL and FNS were 5-800â¯ng/ml and 0.2-30â¯ng/ml respectively. The results of precision and accuracy, linearity, recovery and matrix effect of the method are acceptable. The accuracy was in the range of 92.9%-106.4% and method precision was also good; %CV was less than 8.1%.
Assuntos
Finasterida/sangue , Tadalafila/sangue , Calibragem , Cromatografia Líquida de Alta Pressão/métodos , Glibureto/sangue , Humanos , Reprodutibilidade dos Testes , Extração em Fase Sólida/métodos , Espectrometria de Massas em Tandem/métodosRESUMO
Finasteride (FIN) is a Class II candidate of the Biopharmaceutics Classification System (BCS). The lipophilic cavity of cyclodextrins (CyDs) enables it to construct a non-covalent inclusion complex with different insoluble drugs. Only ß-cyclodextrin (ß-CyD) and hydroxypropyl-ß-CyD (HP-ß-CyD) have been previously examined with FIN. This study aimed to investigate the consistence of FIN with different kinds of ß-CyDs, including dimethyl-ß-cyclodextrin (DM-ß-CyD), carboxymethyl-ß-cyclodextrin (CM-ß-CyD), HP-ß-CyD, sulfobutyl ether-ß-cyclodextrin (SBE-ß-CyD), and ß-CyD, by the coprecipitation method. The resultant inclusion systems were characterized by differential scanning calorimetry, infrared spectroscopy, X-ray diffractometry, and dissolution studies. Moreover, molecular docking for the selected inclusion systems was carried out to explore the suitable arrangements of FIN in the cavity of ß-CyD or its derivatives. The results suggested that the DM-ß-CyD inclusion system gave the higher complexation efficiency for improvement in solubility of FIN and hence enhancement of its bioavailability. Pharmacokinetic parameters displayed a higher absorption rate and higher area under the curve of the FIN/DM-ß-CyD inclusion complex when compared with the drug alone, which indicates an improvement in the absorption and bioavailability of FIN in the DM-ß-CyD inclusion system.
Assuntos
Inibidores de 5-alfa Redutase/farmacocinética , Finasterida/farmacocinética , Simulação de Acoplamento Molecular , beta-Ciclodextrinas/química , Inibidores de 5-alfa Redutase/administração & dosagem , Inibidores de 5-alfa Redutase/sangue , Inibidores de 5-alfa Redutase/química , Administração Oral , Animais , Área Sob a Curva , Disponibilidade Biológica , Varredura Diferencial de Calorimetria , Cristalografia por Raios X , Composição de Medicamentos , Liberação Controlada de Fármacos , Finasterida/administração & dosagem , Finasterida/sangue , Finasterida/química , Meia-Vida , Absorção Intestinal , Taxa de Depuração Metabólica , Modelos Biológicos , Difração de Pó , Coelhos , Solubilidade , Espectrofotometria InfravermelhoRESUMO
This study aimed to use the biocompatibility features of the biodegradable polymers to prepare depot injectable finasteride (FIN) microspheres for the treatment of benign prostatic hyperplasia. FIN microspheres were prepared utilising an emulsion-solvent evaporation/extraction technique. The Box-Behnken experimental design was adopted to optimise the preparation process. FIN plasma levels in albino rabbits were determined after injection with optimised FIN microspheres formula and compared with oral FIN suspension. Results revealed that the optimum microspheres displayed an amended sustained release pattern with lower initial burst. The cumulative FIN % released after 25 days was in the range 27.83-73.18% for F4 and F1, respectively. The optimised formula, with 50.0% (X1), and 22.316% (X2) and 1.38% (X3) showed 6.503 µm, 93.213%, 14.574%, and 64.838% for Y1, Y2, Y3, and Y4, respectively. In vivo studies displayed a sustained release pattern with minimal initial burst release when injected into rabbits.
Assuntos
Preparações de Ação Retardada/química , Finasterida/administração & dosagem , Finasterida/sangue , Agentes Urológicos/administração & dosagem , Agentes Urológicos/sangue , Animais , Materiais Biocompatíveis/química , Caproatos/química , Composição de Medicamentos/métodos , Humanos , Injeções , Ácido Láctico/química , Lactonas/química , Masculino , Ácido Poliglicólico/química , Copolímero de Ácido Poliláctico e Ácido Poliglicólico , Hiperplasia Prostática/tratamento farmacológico , CoelhosRESUMO
OBJECTIVE: In the Prostate Cancer Prevention Trial (PCPT), finasteride reduced the risk of prostate cancer by 25%, even though high-grade prostate cancer was more common in the finasteride group. However, it remains to be determined whether finasteride concentrations may affect prostate cancer risk. In this study, we examined the association between serum finasteride concentrations and the risk of prostate cancer in the treatment arm of the PCPT and determined factors involved in modifying drug concentrations. METHODS: Data for this nested case-control study are from the PCPT. Cases were drawn from men with biopsy-proven prostate cancer and matched controls. Finasteride concentrations were measured using a liquid chromatography-mass spectrometry validated assay. The association of serum finasteride concentrations with prostate cancer risk was determined by logistic regression. We also examine whether polymorphisms in the enzyme target and metabolism genes of finasteride are related to drug concentrations using linear regression. RESULTS AND CONCLUSIONS: Among men with detectable finasteride concentrations, there was no association between finasteride concentrations and prostate cancer risk, low-grade or high-grade, when finasteride concentration was analyzed as a continuous variable or categorized by cutoff points. Since there was no concentration-dependent effect on prostate cancer, any exposure to finasteride intake may reduce prostate cancer risk. Of the twenty-seven SNPs assessed in the enzyme target and metabolism pathway, five SNPs in two genes, CYP3A4 (rs2242480; rs4646437; rs4986910), and CYP3A5 (rs15524; rs776746) were significantly associated with modifying finasteride concentrations. These results suggest that finasteride exposure may reduce prostate cancer risk and finasteride concentrations are affected by genetic variations in genes responsible for altering its metabolism pathway. TRIAL REGISTRATION: ClinicalTrials.gov NCT00288106.
Assuntos
Citocromo P-450 CYP3A/genética , Finasterida/sangue , Finasterida/uso terapêutico , Neoplasias da Próstata/prevenção & controle , Idoso , Estudos de Casos e Controles , Testes Genéticos , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único , Neoplasias da Próstata/sangue , Neoplasias da Próstata/genéticaRESUMO
A specific, fast and sensitive LC-MS/MS assay was developed for the determination of finasteride in human plasma using betamethsone dipropionate as the internal standard (IS). The limit of quantification was 1.0 ng/ml and the method was linear in the range of 1.0-25.0 ng/ml. The retention times were 0.75 min for finasteride and 0.85 min for IS. Method intra-batch precision and accuracy ranged from 3.6 to 7.1%, and 96.6 to 103.9%, respectively. Inter-batch precision ranged from 2.5 to 3.4%, while Inter-batch accuracy ranged from 100.3 to 103.5%. The analytical method was applied to evaluate the pharmacokinetic and relative bioavailability of 2 different pharmaceutical formulations containing 1.0 mg of finasteride. This study evaluated 38 volunteers in a randomized, 2-period crossover study with 7 days washout period between doses. The geometric mean and respective 90% CI of finasteride test/reference percent ratios were 95.68% (91.2 - 104.6%) for Cmax, 97.5% (92.1-103.3%) for AUC0-t and 98.1 (92.67-103.8) for AUC0-inf. Based on the 90% confidence interval of the individual ratios (test formulation/reference formulation) for Cmax and AUC0-inf, it was concluded that the test formulation is bioequivalent to the reference one with respect to the rate and extent of absorption of finasteride.
Assuntos
Finasterida/sangue , Finasterida/farmacocinética , Inibidores de 5-alfa Redutase/sangue , Inibidores de 5-alfa Redutase/farmacocinética , Adolescente , Adulto , Betametasona/análogos & derivados , Betametasona/sangue , Betametasona/farmacocinética , Disponibilidade Biológica , Cromatografia Líquida de Alta Pressão , Estudos Cross-Over , Humanos , Limite de Detecção , Masculino , Pessoa de Meia-Idade , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas em Tandem , Equivalência Terapêutica , Adulto JovemRESUMO
Benign prostatic hyperplasia and prostate cancer can be treated with the 5α-reductase inhibitors, finasteride and dutasteride, when pharmacodynamic biomarkers are useful in assessing response. A novel method was developed to measure the substrates and products of 5α-reductases (testosterone, 5α-dihydrotestosterone (DHT), androstenedione) and finasteride and dutasteride simultaneously by liquid chromatography tandem mass spectrometry, using an ABSciex QTRAP(®) 5500, with a Waters Acquity™ UPLC. Analytes were extracted from serum (500 µL) via solid-phase extraction (Oasis(®) HLB), with (13)C3-labelled androgens and d9-finasteride included as internal standards. Analytes were separated on a Kinetex C18 column (150 × 3 mm, 2.6 µm), using a gradient run of 19 min. Temporal resolution of analytes from naturally occurring isomers and mass +2 isotopomers was ensured. Protonated molecular ions were detected in atmospheric pressure chemical ionisation mode and source conditions optimised for DHT, the least abundant analyte. Multiple reaction monitoring was performed as follows: testosterone (m/z 289 â 97), DHT (m/z 291 â 255), androstenedione (m/z 287 â 97), dutasteride (m/z 529 â 461), finasteride (m/z 373 â 317). Validation parameters (intra- and inter-assay precision and accuracy, linearity, limits of quantitation) were within acceptable ranges and biological extracts were stable for 28 days. Finally the method was employed in men treated with finasteride or dutasteride; levels of DHT were lowered by both drugs and furthermore the substrate concentrations increased.
Assuntos
Inibidores de 5-alfa Redutase/sangue , Androgênios/sangue , Cromatografia Líquida/métodos , Neoplasias da Próstata/sangue , Espectrometria de Massas em Tandem/métodos , Inibidores de 5-alfa Redutase/farmacocinética , Inibidores de 5-alfa Redutase/farmacologia , Androgênios/farmacocinética , Androgênios/farmacologia , Androstenodiona/sangue , Androstenodiona/farmacocinética , Androstenodiona/farmacologia , Azasteroides/sangue , Azasteroides/farmacocinética , Azasteroides/farmacologia , Di-Hidrotestosterona/sangue , Di-Hidrotestosterona/farmacocinética , Di-Hidrotestosterona/farmacologia , Dutasterida , Finasterida/sangue , Finasterida/farmacocinética , Finasterida/farmacologia , Humanos , Masculino , Neoplasias da Próstata/tratamento farmacológico , Extração em Fase Sólida/métodos , Testosterona/sangue , Testosterona/farmacocinética , Testosterona/farmacologia , Distribuição TecidualRESUMO
PURPOSE: The primary aim of this study was to evaluate whether there was clinically significant pharmacokinetic (PK) interaction between finasteride and tamsulosin in healthy Chinese male subjects. METHODS: This was an open-label, randomized, 3-period, crossover study. Subjects received single and multiple doses of 5 mg finasteride alone, single and multiple doses of 0.2 mg tamsulosin hydrochloride sustained-release capsule alone, and single and multiple doses of 5 mg finasteride with 0.2 mg tamsulosin hydrochloride, in an order determined by a computerized randomization schedule. Blood samples were collected up to 48 hours after dosing on study day 1 and up to 24 hours after dosing on study day 9 for determination of plasma concentrations with a validated LC-MS/MS method. Pharmacokinetic parameters were estimated via noncompartmental methods. Tolerability was evaluated by monitoring adverse events, laboratory assays, vital signs, and 12-lead ECG. FINDINGS: Fifteen subjects were enrolled, and 14 completed the study. The geometric mean ratios (GMRs) (90% CIs) of AUC(τ,ss) and C(max,ss) values of finasteride at steady state between coadministration of finasteride and tamsulosin hydrochloride and finasteride alone were 1.14 (1.05-1.23) and 1.06 (0.99-1.14), respectively. The GMRs (90% CIs) for AUC(0-t) and C(max) values of finasteride for a single dose of coadministration of finasteride and tamsulosin hydrochloride and finasteride alone were 1.02 (0.94-1.11) and 1.06 (1.01-1.11), respectively. The GMRs (90% CIs) for AUC(τ,ss) and C(max,ss) values of tamsulosin at steady-state for coadministration of finasteride and tamsulosin hydrochloride and tamsulosin hydrochloride alone were 1.18 (1.05-1.33) and 1.23 (1.06-1.43), respectively. The GMRs (90% CIs) for AUC(0-t) and C(max) values of tamsulosin for a single dose of coadministration of finasteride and tamsulosin hydrochloride and tamsulosin hydrochloride alone were 1.04 (0.97-1.10) and 1.04 (0.98-1.11), respectively. Statistical analyses confirmed that the 90% CIs for these PK parameters were within the predefined not clinically significant PK drug-drug interaction effect boundaries (0.5-2.0) in this study. If comparing the findings with narrower boundaries (0.8-1.25), the conclusion may not be supportive for tamsulosin hydrochloride. During the study, a total of 4 adverse events were reported in 3 subjects including allergic reaction, abnormal findings on an ECG, a slight increase in alanine aminotransferase, and a positive result on glucose urine test. IMPLICATIONS: Both finasteride and tamsulosin hydrochloride were well tolerated. Coadministration of finasteride and tamulosin hydrochloride seems unlikely to lead to a clinically significant PK drug-drug interaction, after a single dose and at steady state.
Assuntos
Finasterida/farmacocinética , Sulfonamidas/farmacocinética , Agentes Urológicos/farmacocinética , Adulto , Área Sob a Curva , Povo Asiático , Cápsulas , Estudos Cross-Over , Preparações de Ação Retardada , Combinação de Medicamentos , Interações Medicamentosas , Finasterida/administração & dosagem , Finasterida/efeitos adversos , Finasterida/sangue , Voluntários Saudáveis , Humanos , Masculino , Pessoa de Meia-Idade , Sulfonamidas/administração & dosagem , Sulfonamidas/efeitos adversos , Sulfonamidas/sangue , Tansulosina , Espectrometria de Massas em Tandem/métodos , Agentes Urológicos/administração & dosagem , Agentes Urológicos/efeitos adversos , Agentes Urológicos/sangue , Adulto JovemRESUMO
In hair follicle (Hf) cells, the type-2 5-α-reductase enzyme, implicated in androgenetic alopecia, is selectively inhibited by finasteride (FNS). Because an effective topical formulation to deliver FNS to Hf is currently unavailable, this investigation aimed at evaluating in vitro FNS skin permeation and retention through and into hairless rat and human abdominal skin. Four hydroxypropyl chitosan (HPCH)-based formulations (P-08-012, P-08-016, P-08-063, and P-08-064) and one anhydrous formulation without HPCH (P-10-008) were tested. The pharmacokinetics in plasma and skin after application of P-08-016 or P-10-008 on dorsal rat skin with single and repeated doses was investigated. P-08-016 performed the best in driving FNS to the reticular dermis without producing a high transdermal flux. Neither the in vivo single nor the repeated dose experiments produced plasma levels of FNS and no differences were found between formulations concerning skin retention. No increase in the amount of drug retained in the skin was obtained with the repeated dose experiment. In conclusion, the HPCH-based formulation P-08-016 might represent an alternative to systemic therapy for its ability to promote a cutaneous depot of FNS in the region of hair bulbs, minimizing systemic absorption even after repeated treatments.
Assuntos
Inibidores de 5-alfa Redutase/farmacocinética , Finasterida/farmacocinética , Absorção Cutânea , Inibidores de 5-alfa Redutase/administração & dosagem , Inibidores de 5-alfa Redutase/sangue , Administração Cutânea , Alopecia/tratamento farmacológico , Animais , Finasterida/administração & dosagem , Finasterida/sangue , Humanos , Masculino , Ratos , Ratos Pelados , Pele/metabolismoRESUMO
1. The metabolite profile of the 5α-reductase type II inhibitor finasteride has been studied in pig plasma, urine and bile using high-resolution mass spectrometry. The porcine biotransformation products were compared to those formed by human liver microsomes and to literature data of recently identified human in vivo metabolites. The objective of this study was to gain further evidence for the validity of using pigs for advanced, invasive drug-drug interaction studies that are not possible to perform in humans. 2. The use of high-resolution mass spectrometry with accurate mass measurements enabled identification of the metabolites by calculation of their elemental compositions as well as their fragmentation patterns. 3. There was an excellent match between the porcine and human metabolic profiles, corroborating the pig as a model of human drug metabolism. The glucuronides of the two recently described human hydroxylated metabolites MX and MY and the carboxylated metabolite M3 were identified as the major biotransformation products of finasteride in pig urine and bile. 4. Furthermore, the CYP enzymes involved in the formation of the hydroxylated metabolites were characterized. Human recombinant CYP3A4 could produce the two major hydroxylated metabolites MX and MY, whereas human recombinant CYP2D6 formed MY only.
Assuntos
Finasterida/análise , Finasterida/metabolismo , Espectrometria de Massas/métodos , Desintoxicação Metabólica Fase II , Desintoxicação Metabólica Fase I , Sus scrofa/sangue , Sus scrofa/urina , Animais , Bile , Cromatografia Líquida de Alta Pressão , Sistema Enzimático do Citocromo P-450/metabolismo , Finasterida/sangue , Finasterida/urina , Humanos , Microssomos Hepáticos/metabolismo , Peso Molecular , Padrões de ReferênciaRESUMO
Deferral of blood donors taking teratogenic drugs is critical. From March 2008 to January 2009, we analysed stored blood specimens from donors who had taken teratogenic drugs and whose blood was transfused to women of childbearing age to determine the plasma concentration at the time of donation using high-performance liquid chromatography. In total, 167 specimens were examined. The numbers of specimens exceeding the quantification limit were 7, 39, 4, 2 and 1 for finasteride, isotretinoin, acitretin, etretinate and dutasteride, respectively. Finasteride was beyond the recommended drug deferral period in one specimen. These results may help create practical deferral policies.
Assuntos
Doadores de Sangue , Teratogênicos/análise , Reação Transfusional , Acitretina/sangue , Adulto , Etretinato/sangue , Feminino , Finasterida/sangue , Humanos , Adulto JovemRESUMO
Finasteride (FIN), a widely used medication for the treatment of androgen-dependent diseases, blocks the conversion of testosterone to a more potent androgen, dihydrotestosterone (DHT). In this study, we investigated a dosing time-dependent effect and safety of FIN in rats. Androgen receptor (AR) mRNA and nuclear protein levels exhibited clear daily rhythms with the peak during the dark period in the prostate and during the light period in the liver. Repeated oral administration of FIN (5 or 100 mg/kg) at 3 h after lights on (HALO) for 2 weeks decreased serum DHT concentration throughout a 24-h period, whereas the dosing of the agent at 15 HALO decreased its level only transiently even in the higher dose group. FIN caused laboratory abnormalities in the 3 HALO group but not in the 15 HALO group. However, the effect of FIN on the prostate weight was not influenced by the dosing time. These results suggest that the safety, but not effect, of FIN depends on its dosing time in rats. The dosing of FIN in the active period might be a rational dosage regimen, which is needed to be confirmed in human subjects.
Assuntos
Finasterida/efeitos adversos , Finasterida/uso terapêutico , Animais , Ritmo Circadiano/efeitos dos fármacos , Ritmo Circadiano/fisiologia , Relação Dose-Resposta a Droga , Esquema de Medicação , Finasterida/sangue , Humanos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Próstata/efeitos dos fármacos , Próstata/metabolismo , Ratos , Ratos Wistar , Receptores Androgênicos/biossíntese , Receptores Androgênicos/sangue , Receptores Androgênicos/genética , Resultado do TratamentoRESUMO
In this study, an attempt was made to describe and validate liquid chromatography-electrospray ionization tandem mass spectrometry as a fast, sensitive and reproducible method for determining finasteride in human plasma. Finasteride and internal standard (pantoprazole) were extracted by liquid-liquid extraction using methyl tert-butyl ether. Separation was performed by using a flow rate gradient on a reverse phase C18 column at 25°C. The mobile phase consisted of methanol-water (70:30, v/v) containing 0.5% anhydrous formic acid. The protonated analytes were quantitated in positive ionization by multiple reaction monitoring in mass spectrometry. The mass transitions are m/z 373.4â305.3 and 384.1â200.0 for finasteride and pantoprazole, respectively. The method had a run time of 3.6 min and a linear calibration curve at a range of 0.2-100 ng mL(-1) (r2=0.9958). The lower limit of quantification was 0.2 ng mL(-1). The extraction recoveries of finasteride from the biological matrix were more than 82.7%, and the intra- and inter-day precision of the assay at four concentrations were 2.4-8.0% with an accuracy of 94.3-105.8%. The developed method requires less plasma (0.1 mL), but has high sensitivity. The validated method has been successfully used to analyze human plasma samples in pharmacokinetic or bioequivalence studies.
Assuntos
Inibidores de 5-alfa Redutase/sangue , Cromatografia Líquida/métodos , Finasterida/sangue , Espectrometria de Massas por Ionização por Electrospray/métodos , Inibidores de 5-alfa Redutase/farmacocinética , Administração Oral , Adulto , Finasterida/farmacocinética , Humanos , Masculino , Reprodutibilidade dos Testes , Espectrometria de Massas em Tandem/métodos , Equivalência Terapêutica , Adulto JovemRESUMO
A rapid, specific, and sensitive method utilizing reversed-phase ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed and validated to determine finasteride levels in human plasma. The plasma samples were prepared by liquid-liquid extraction with ethyl acetate, evaporation, and reconstitution. MS/MS analyses were performed on a triple-quadrupole tandem mass spectrometer by monitoring protonated parent-->daughter ion pairs at m/z 373-->305 for finasteride and m/z 237-->194 for carbamazepine (internal standard, IS). The method was validated with respect to linearity, recovery, specificity, accuracy, precision, and stability. The method exhibited a linear response from 0.1 to 30 ng/mL (r(2)>0.998). The limit of quantitation for finasteride in plasma was 0.1 ng/mL. The relative standard deviation (RSD) of intra- and inter-day measurements was less than 15% and the method was accurate within -6.0% to 2.31% at all quality-control levels. The mean extraction recovery was higher than 83% for finasteride and 84% for the IS. Plasma samples containing finasteride were stable under the three sets of conditions tested and the processed samples were stable up to 29 h in an autosampler at 5 degrees C. Detection and quantitation of both analytes within 3 min make this method suitable for high-throughput analyses. The method was successfully applied to a pharmacokinetic study of finasteride in healthy volunteers following oral administration.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Finasterida/sangue , Espectrometria de Massas em Tandem/métodos , Adulto , Finasterida/farmacocinética , Humanos , MasculinoRESUMO
Novel sample preparation approaches for HPLC bioanalysis based on the phenomenon that acetonitrile can be separated from water by adding salts or cooling at subzero temperatures have been reported. These two methods are superior to conventional liquid-liquid extraction since the separated acetonitrile phase can be directly injected to the RP-LC system. However, the salting-out method suffers from a potential problem that the remained salt in the acetonitrile phase may harm the MS detector, while the subzero-temperature method is troublesome to operate. Here, we have reported a similar phase separation phenomenon that the acetonitrile aqueous mixture can be separated by adding a hydrophobic solvent; and capitalising on this phase transition phenomenon, we have proposed an alternative approach, named solvent induced phase transition extraction (SIPTE), to extract drug from plasma for HPLC-MS analysis. The proposed SIPTE method is much simpler and avoids contaminating the MS detector. Three structurally diverse drugs were selected as test compounds to design the SIPTE method and to validate the efficiency of this method. The four goals of plasma sample pretreatment for HPLC-MS analysis, i.e. removal of proteins, removal of other low-molecular interferences, preconcentration of the analytes of interest, and matching the sample solvent with the HPLC-MS system, can be rapidly performed in a very simple step by using the SIPTE method.
Assuntos
Acetonitrilas/química , Fracionamento Químico/métodos , Cromatografia Líquida de Alta Pressão/métodos , Espectrometria de Massas/métodos , Preparações Farmacêuticas/sangue , Clorofórmio/química , Diterpenos/sangue , Diterpenos/isolamento & purificação , Finasterida/sangue , Finasterida/isolamento & purificação , Humanos , Interações Hidrofóbicas e Hidrofílicas , Modelos Moleculares , Preparações Farmacêuticas/isolamento & purificação , Piperazinas/sangue , Piperazinas/isolamento & purificação , Purinas/sangue , Purinas/isolamento & purificação , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Citrato de Sildenafila , Sulfonas/sangue , Sulfonas/isolamento & purificaçãoRESUMO
A sensitive, rapid and specific liquid chromatography-electrospray ionization-tandem mass spectrometry method was developed and validated for the determination of aristolochic acid-I (AA-I) in rat plasma. Finasteride was used as the internal standard (IS). The analyte was separated on a Zorbax Eclipse XDB-C(18) column by isocratic elution with methanol-10 mM ammonium acetate (75:25, v/v, pH = 7.3) at a flow rate of 0.25 mL/min, and analyzed by mass spectrometry in positive multiple reaction monitoring mode. The precursor-to-product ion transitions of m/z 359.0 --> 298.2 and m/z 373.1 --> 305.2 were used to detect AA-I and IS, respectively. Good linearity was achieved over a range of 0.4-600 ng/mL. Intra- and inter-day precisions measured as relative standard deviation were less than 13.5%, and accuracy ranged from 94.2 to 97.5%. The developed method was successfully applied in the pharmacokinetic study of AA-I in rats.
Assuntos
Ácidos Aristolóquicos/sangue , Animais , Área Sob a Curva , Ácidos Aristolóquicos/administração & dosagem , Ácidos Aristolóquicos/farmacocinética , Calibragem , Cromatografia Líquida de Alta Pressão , Inibidores Enzimáticos/sangue , Finasterida/sangue , Meia-Vida , Indicadores e Reagentes , Intubação Gastrointestinal , Plasma/química , Controle de Qualidade , Ratos , Ratos Sprague-Dawley , Padrões de Referência , Reprodutibilidade dos Testes , Soluções , Espectrometria de Massas em TandemRESUMO
The aim of this study was to investigate what the consequences of induced drug metabolism, caused by St. John's wort (SJW, Hypericum perforatum) treatment, would have on the plasma, biliary and urinary pharmacokinetics of finasteride and its two previously identified phase I metabolites (hydroxy-finasteride and carboxy-finasteride). Twelve healthy men were administered 5mg finasteride directly to the intestine via a catheter with a multi-channel tubing system, Loc-I-Gut, before and after 14 days SJW (300mg b.i.d, hyperforin 4%) treatment. Bile samples were withdrawn via the Loc-I-Gut device from the proximal jejunum. LC-ESI-MS/MS was used to analyze finasteride and its metabolites in plasma, bile and urine. HPLC-UV was used to analyze hyperforin in plasma. The herbal treatment significantly reduced the peak plasma concentration (C(max)), the area under the plasma concentration-time curve (AUC(0-24h)) and the elimination half-life (t(1/2)) of finasteride. The geometric mean ratios (90% CI) were 0.42 (0.36-0.49), 0.66 (0.56-0.79) and 0.54 (0.48-0.61), respectively. Finasteride was excreted unchanged to a minor extent into bile and urine. Hydroxy-finasteride was not detected in plasma, bile or urine. Carboxy-finasteride was quantified in all three compartments and its plasma pharmacokinetics was significantly affected by SJW treatment. Hyperforin concentration in plasma was 21+/-7ng/ml approximately 12h after the last dose of the 14 days SJW treatment. In conclusion, SJW treatment for 2 weeks induced the metabolism of finasteride and caused a reduced plasma exposure of the drug. New knowledge was gained about the biliary and urinary excretion or the drug and its metabolites.
Assuntos
Sistema Biliar/metabolismo , Inibidores Enzimáticos/farmacocinética , Finasterida/farmacocinética , Hypericum , Adulto , Compostos Bicíclicos com Pontes/sangue , Cromatografia Líquida de Alta Pressão , Inibidores Enzimáticos/sangue , Inibidores Enzimáticos/urina , Finasterida/sangue , Finasterida/urina , Meia-Vida , Humanos , Masculino , Floroglucinol/análogos & derivados , Floroglucinol/sangue , Valores de Referência , Espectrometria de Massas por Ionização por Electrospray , Espectrofotometria Ultravioleta , Espectrometria de Massas em Tandem , Terpenos/sangueRESUMO
An analytical method is developed and validated for the quantitative determination of finasteride, a potent 5 alpha-reductase inhibitor, in human plasma. Calibration curves are linear in the concentration range of 1 to 100 ng/mL. Sample pretreatment involves a liquid-liquid extraction with ethyl acetate using 0.2 mL aliquots of plasma. Finasteride and the internal standard (beclomethasone) are separated on a Waters Symmetry Shield RP18 column (50 x 2.1 mm, 3.5 microm) and eluted using a gradient mobile phase composed of acetonitrile and 10mM ammonium acetate with 0.1% formic acid. The column eluant is monitored by mass spectrometry with electrospray ionization. A complete validation of the method is performed. For quality control samples at three different concentrations that were analyzed in quintuplicate, on six separate occasions, the accuracy and precision range from 95.2% to 101% and 3.4% to 7.3%, respectively. The developed method is subsequently applied to measure the steady state finasteride concentration of patients who participated in the Prostate Cancer Prevention Trial.
Assuntos
Inibidores de 5-alfa Redutase , Cromatografia Líquida de Alta Pressão/métodos , Finasterida/sangue , Espectrometria de Massas/métodos , Estabilidade de Medicamentos , Finasterida/uso terapêutico , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias da Próstata/prevenção & controle , Ensaios Clínicos Controlados Aleatórios como Assunto/métodos , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
A fast, accurate, sensitive, selective and reliable method using reversed-phase high-performance liquid chromatography-mass spectrometry coupling with an electrospray ionization interface was developed and validated for the determination of finasteride in human plasma. After deprotienation with acetonitrile, centrifugation, evaporation to dryness and dissolving in mobile phase, satisfactory separation was achieved on a Hypersil-Keystone C(18) reversed-phase column using a mobile phase consisting of acetonitrile-water (46:54, v/v), 0.1% acetic acid and 0.1% trifluoracetic acid. Carbamazepine (IS) was used as internal standard. This method involved the use of the [M+H](+) ions of finasteride and IS at m/z 373 and 237 with the selective ion monitoring (SIM) mode. The calibration curve was linear in the range of 0.2-120 ng ml(-1). The limit of quantification for finasteride in plasma was 0.2 ng ml(-1) with good accuracy and precision. The intra-assay precision and accuracy were in the range of 2.1-11.2% and -1.3% to 8.5%, respectively. The inter-assay precision and accuracy were in the order of 3.4-12.1% and -1.5% to 11.5%, respectively. The mean sample extract recoveries of the method were higher than 85% and 74% for finasteride and internal standard (IS), respectively. The assay has been successfully used to estimate the pharmacokinetics of finasteride after oral administration of a 5mg tablet of finasteride to 24 healthy volunteers.
Assuntos
Cromatografia Líquida/métodos , Inibidores Enzimáticos/sangue , Inibidores Enzimáticos/farmacocinética , Finasterida/sangue , Finasterida/farmacocinética , Espectrometria de Massas por Ionização por Electrospray/métodos , Administração Oral , Adolescente , Adulto , Carbamazepina/química , Cromatografia Líquida/instrumentação , Inibidores Enzimáticos/administração & dosagem , Inibidores Enzimáticos/química , Finasterida/administração & dosagem , Finasterida/química , Humanos , Masculino , Estrutura Molecular , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Comprimidos , Fatores de TempoAssuntos
Inibidores de 5-alfa Redutase , Antagonistas Adrenérgicos alfa/uso terapêutico , Azasteroides/uso terapêutico , Finasterida/uso terapêutico , Hiperplasia Prostática/tratamento farmacológico , Antagonistas Adrenérgicos alfa/administração & dosagem , Azasteroides/administração & dosagem , Azasteroides/sangue , Ensaios Clínicos Fase II como Assunto , Quimioterapia Combinada , Dutasterida , Finasterida/administração & dosagem , Finasterida/sangue , Humanos , Masculino , Guias de Prática Clínica como Assunto , Fatores de TempoRESUMO
AIM: To develop an HPLC-MS assay for determination of finasteride in human plasma and to investigate the bioequivalence in healthy volunteers. METHODS: After alkalization with sodium hydroxide, plasma was extracted with ethyl acetate and separated using a C18 column with a mobile phase of methanol-water (85:15). LC-ESI-MS was performed in the selected ion monitoring (SIM) mode using target ions at m/z 395 for finasteride and m/z 407 for the IS. The fragmentor voltage was 120 V. A randomized crossover design was performed in 20 healthy volunteers. In the two study periods, a single 10 mg dose of each tablet was administered to each volunteer. RESULTS: Calibration curves were linear over the range 1-200 micrograms.L-1 (r = 0.9986). The limit of determination for finasteride in plasma was 0.05 microgram.L-1. The recovery of finasteride from plasma was in the range of 85.9%-98.7%. The results of variance analysis and two one-side t-test showed that there was no significant difference between the two formulations in the AUC and Cmax. CONCLUSION: The assay was proved to be sensitive, accurate and convenient. The two formulations were bioequivalent.