RESUMO
The effect of K and Mg salts of aspartic acid (Cardilan) on the serum concentration of selected proteins and phagocytic activity in aging male Wistar rats was investigated. Cardilan was administered in tap water for 7 days a month for 3 months before the last observed interval (12, 18 and 24 month). In a part of animals, the aging process was accelerated by sublethally gamma-irradiation. The administration of Cardilan slowed down the changes in the concentration of prealbumin, albumin, haptoglobin, haemopexin, C3 complement in non-irradiated rats (DC). This effect was extended to the changes in transferrin level in irradiated rats (IDC). The phagocytic activity in both DC, IDC rats was lower compared with controls drinking water (DW, IDW), but not significantly. The effect of Cardilan administration appears to be the greatest in 24-month-old rats, when the treated animals survived better by 25% in IDC group and by 26% better in DC rats, compared with those of the same age controls. Potassium and magnesium salts of aspartates are suitable compounds for life prolongation in the experimental conditions.
Assuntos
Envelhecimento/efeitos dos fármacos , Ácido Aspártico/farmacologia , Proteínas Sanguíneas/efeitos dos fármacos , Envelhecimento/metabolismo , Envelhecimento/efeitos da radiação , Animais , Proteínas Sanguíneas/metabolismo , Proteínas Sanguíneas/efeitos da radiação , Complemento C3/efeitos dos fármacos , Complemento C3/metabolismo , Complemento C3/efeitos da radiação , Raios gama/efeitos adversos , Haptoglobinas/efeitos dos fármacos , Haptoglobinas/metabolismo , Haptoglobinas/efeitos da radiação , Hemopexina/efeitos dos fármacos , Hemopexina/metabolismo , Hemopexina/efeitos da radiação , Imunidade Inata/efeitos dos fármacos , Imunidade Inata/imunologia , Imunidade Inata/efeitos da radiação , Masculino , Neutrófilos/efeitos dos fármacos , Neutrófilos/metabolismo , Neutrófilos/efeitos da radiação , Fagocitose/efeitos dos fármacos , Fagocitose/imunologia , Fagocitose/efeitos da radiação , Ratos , Ratos Wistar , Albumina Sérica/efeitos dos fármacos , Albumina Sérica/metabolismo , Albumina Sérica/efeitos da radiação , Taxa de Sobrevida , Transferrina/efeitos dos fármacos , Transferrina/metabolismo , Transferrina/efeitos da radiaçãoRESUMO
Three types of hepatic proteins, a heme-binding Z protein, a mixture of the glutathione S-transferases and a cytochrome P450 isozyme, were shown to be susceptible to photodynamic cross-linking and loss in antigenicity by naturally occurring porphyrins. At 50 microM, uroporphyrin caused the most and protoporphyrin the least photodecomposition. Hemopexin, a specific serum heme carrier, was photodecomposed but no cross-linking was detected. Heme and scavengers of singlet oxygen partially prevented protein photodecomposition.
Assuntos
Proteínas de Transporte/efeitos da radiação , Hemeproteínas , Luz , Fígado/análise , Porfirinas/farmacologia , Animais , Proteínas de Transporte/imunologia , Coproporfirinas/farmacologia , Sistema Enzimático do Citocromo P-450/efeitos da radiação , Epitopos/efeitos da radiação , Glutationa Transferase/efeitos da radiação , Heme/farmacologia , Proteínas Ligantes de Grupo Heme , Hemopexina/efeitos da radiação , Histidina/farmacologia , Imunoeletroforese , Fotoquímica , Protoporfirinas/farmacologia , Ratos , Temperatura , Fatores de Tempo , Raios Ultravioleta , Uroporfirinas/farmacologiaRESUMO
Changes in the concentration of some serum proteins are determined by quantitative two-dimensional immunoelectrophoresis in rats subjected to continuous irradiation with 400R/day for 11 days. At this dose rate albumin levels were decreased by 36% and IGG levels by 70% in comparison with the control (C2) values. The concentration of haptoglobin, haemopexin and caeruloplasmin was increased by 75-140% on day 10 as compared with the control (C2) values.