RESUMO
BACKGROUND: Infection of Echinococcus multilocularis causes in humans the alveolar echinococcosis. Although the infection has world-wide distribution it is rarely detected. Diagnosis of alveococcosis is difficult because of not typical clinical picture and irregular results of radiological examinations suggesting neoplasmatic process which begins in the liver tissue or in the biliary tracts. The parasitic growth is slow, so the illness is quite often established in late invasion period. Treatment of long-lasting and late diagnosed infection is difficult and requires cooperation of parasitologists together with surgeons to avoid life-threatening organ dysfunction. CASE PRESENTATION: We describe a young male patient, diagnosed, according to the radiological, immunological and histological examination results, infection of Echinococcus multilocularis, who was treated with not radical resection of pathologic mass together with persistent albendazole intake. The right hepatectomy was performed. In addition, visible cysts were removed from the left lobe of the liver in nonanatomical resection and suspicious calcified lesions in hepatoduodenal ligament were also removed. After the operation portal hypertension, with splenomegaly and symptoms of the liver cirrhosis occurred (thrombocytopenia, collateral venous circulation, first degree varices oesophagii). The portal hypertension probably could be a result of incomplete surgery due to extended parasitic infection and liver anathomical changes due to performed procedures, because the portal hypertension and it's further complications had not been observed before the operation. CONCLUSIONS: Echinococcus multilocularis should be taken under consideration in differential diagnosis of irregular lesions within the liver. Lon-lasting invasion could be responsible for the irreversible secondary liver changes such as cirrhosis and portal hypertension. The surgery treatment (treatment of choice) is difficult and it's results depends on the invasion period the patient is operated on. After the surgery the patient requires careful follow - up, to detect early complications.
Assuntos
Equinococose Hepática/cirurgia , Echinococcus multilocularis , Hepatectomia/efeitos adversos , Hipertensão Portal/parasitologia , Complicações Pós-Operatórias/parasitologia , Adulto , Animais , Equinococose Hepática/parasitologia , Humanos , MasculinoRESUMO
INTRODUCTION: Schistosomiasis associated pulmonary arterial hypertension belongs to group 1 of the pulmonary hypertension classification and should be considered in any patient with pulmonary hypertension returning from an endemic area. CASE REPORT: A 17-year-old patient was hospitalized for pulmonary hypertension detected during the initial assessment of viral hepatitis B-related cirrhosis with portal hypertension. The initial assessment established the diagnosis of pulmonary hypertension secondary to viral hepatitis B-cirrhosis. The patient's hepatic and haemodynamic condition deteriorated and he was treated with intravenous epoprostenol. This allowed subsequent performance of a liver transplantation. Epoprostenol could then be discontinued. Unexpectedly, histology of the liver explant revealed florid schistosomiasis in addition to hepatitis B cirrhosis. CONCLUSION: The diagnosis of pulmonary arterial hypertension associated with schistosomiasis may be difficult. It is necessary to repeat the serological studies and, sometimes, to obtain a rectal biopsy. The treatment of pulmonary arterial hypertension associated with schistosomiasis is based on specific therapies and antiparasitic treatment.
Assuntos
Hipertensão Pulmonar/diagnóstico , Hipertensão Pulmonar/etiologia , Esquistossomose mansoni/complicações , Esquistossomose mansoni/diagnóstico , Adolescente , Animais , Diagnóstico Diferencial , Epoprostenol/administração & dosagem , Hepatite B/complicações , Hepatite B/diagnóstico , Hepatite B/parasitologia , Hepatite B/terapia , Humanos , Hipertensão Portal/diagnóstico , Hipertensão Portal/parasitologia , Hipertensão Portal/terapia , Hipertensão Pulmonar/parasitologia , Hipertensão Pulmonar/terapia , Transplante de Fígado , Masculino , Praziquantel/administração & dosagem , Schistosoma mansoni , Esquistossomose mansoni/terapiaRESUMO
OBJECTIVE: To understand the clinical characteristics of newly discovered advanced schistosomiasis patients in Dongpo District, Meishan City, Sichuan Province, so as to provide the reference for enhancing the clinicians' awareness for diagnosis and treatment of this disease and improving the therapeutic effect. METHODS: The data of medical records and schedule of case survey of 16 newly discovered advanced schistosomiasis patients in Dongpo District, Meishan City, Sichuan Province were collected and analyzed. RESULTS: The mean age of the 16 newly discovered advanced schistosomiasis patients was 63 years, and there were 10 cases at ages of over 60 years. The 16 cases included 8 men and 8 women, and 10 cases were detected in the historical hyper-endemic areas. There were 11 cases with an ascitic type of advanced schistosomiasis, 4 cases with a megalosplenia type, and one case with a colon proliferation type. The primary initial symptoms mainly included ascites, splenomegaly, hypersplenism and portal hypertension. Following inpatient treatments, 15 cases achieved clinical improvments. CONCLUSIONS: It is necessary to enhance the prevention and control of advanced schistosomiasis. If the patients with portal hypertension, hepatosplenomegaly, upper gastrointestinal hemorrhage visit a doctor, the clinicians should pay much attention to advanced schistosomiasis and they need to comprehensively analyze the clinical data in combination with the epidemiological information, clinical features and laboratory examinations, and make a correct diagnosis and give treatments timely.
Assuntos
Esquistossomose/diagnóstico , Ascite/parasitologia , China , Cidades , Feminino , Hospitalização , Humanos , Hipertensão Portal/parasitologia , Masculino , Pessoa de Meia-Idade , Esplenomegalia/parasitologiaAssuntos
Coinfecção , Vírus da Hepatite B/patogenicidade , Hepatite B Crônica/complicações , Hipertensão Portal/parasitologia , Hipertensão Portal/virologia , Schistosoma mansoni/patogenicidade , Esquistossomose mansoni/complicações , Animais , Biópsia , Hepatite B Crônica/diagnóstico , Hepatite B Crônica/virologia , Humanos , Hipertensão Portal/diagnóstico , Hipertensão Portal/fisiopatologia , Pessoa de Meia-Idade , Pressão na Veia Porta , Esquistossomose mansoni/diagnóstico , Esquistossomose mansoni/parasitologiaRESUMO
The role of hydrogen sulfide (H2S) in portal hypertension (PH)-induced esophagus-gastric junction vascular lesions in rabbits was observed. The rabbit PH models were established. The animals were randomly divided into the following groups: normal, PH, PH+sodium hydrosulfide (PH+S), PH+propargylglycine (PH+PPG). The plasma H2S levels, apoptosis of esophageal-gastric junction vascular smooth muscle cells, and the expression of nuclear transcription factor-κB (NF-κB), p-AKT, IκBa and Bcl-2 were detected. The cystathionine γ lyase (cystathionine-gamma-splitting enzyme, CSE) in the junction vascular tissue was measured. The results showed that the plasma H2S levels and the CSE expression levels had statistically significant difference among different groups (P<0.05). As compared with PH group, plasma H2S levels were declined obviously (11.9±4.2 vs. 20.6±4.5, P<0.05), and CSE expression levels in the junction vascular tissue were notably reduced (1.7±0.6 vs. 2.8±0.8, P<0.05), apoptosis rate of vascular smooth muscle cells per unit area was significantly decreased (0.10±0.15 vs. 0.24±0.07, P<0.05), and the expression levels of p-AKT and NF-κB were significantly decreased (2.31±0.33 vs. 3.04±0.38, P<0.05; 0.33±0.17 vs. 0.51±0.23, P<0.05), however, IκBa and Bcl-2 expression increased obviously (5.57±0.17 vs. 3.67±0.13, P<0.05; 0.79±0.29 vs. 0.44±0.36, P<0.05) in PH+PPG group. As compared with PH group, H2S levels were notably increased (32.7±7.3 vs. 20.6±4.5, P<0.05), the CSE levels in the junction vascular tissue were significantly increased (6.3±0.7 vs. 2.8±0.8, P<0.05), apoptosis rate of vascular smooth muscle cells per unit area was significantly increased (0.35±0.14 vs. 0.24±0.07, P<0.05), and the expression levels of p-AKT and NF-κB were significantly increased (4.29±0.49 vs. 3.04±0.38, P<0.05; 0.77±0.27 vs. 0.51±0.23, P<0.05), yet IκBa and Bcl-2 expression decreased significantly (3.23±0.24 vs. 3.67±0.13, P<0.05; 0.31±0.23 vs. 0.48±0.34, P<0.05) in PH+S group. It is concluded that esophagus-gastric junction vascular lesions happen under PH, and apoptosis of smooth muscle cells is declined. H2S can activate NF-κB by the p-AKT pathway, leading to the down-regulation of Bcl-2, eventually stimulating apoptosis of vascular smooth muscle cells, easing PH. H2S/CSE system may play an important role in remission of PH via the AKT-NF-κB pathway.
Assuntos
Anti-Hipertensivos/farmacologia , Sulfeto de Hidrogênio/farmacologia , Hipertensão Portal/tratamento farmacológico , NF-kappa B/agonistas , Proteínas Proto-Oncogênicas c-akt/agonistas , Esquistossomose Japônica/tratamento farmacológico , Alcinos/farmacologia , Animais , Apoptose/efeitos dos fármacos , Cistationina gama-Liase/genética , Cistationina gama-Liase/metabolismo , Modelos Animais de Doenças , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/metabolismo , Células Endoteliais/parasitologia , Esôfago/irrigação sanguínea , Esôfago/efeitos dos fármacos , Esôfago/patologia , Regulação da Expressão Gênica , Glicina/análogos & derivados , Glicina/farmacologia , Hipertensão Portal/complicações , Hipertensão Portal/genética , Hipertensão Portal/parasitologia , Junções Intercelulares/efeitos dos fármacos , Junções Intercelulares/metabolismo , Junções Intercelulares/parasitologia , Masculino , Músculo Liso Vascular/efeitos dos fármacos , Músculo Liso Vascular/metabolismo , Músculo Liso Vascular/parasitologia , Inibidor de NF-kappaB alfa/genética , Inibidor de NF-kappaB alfa/metabolismo , NF-kappa B/genética , NF-kappa B/metabolismo , Sistema Porta/efeitos dos fármacos , Sistema Porta/metabolismo , Sistema Porta/parasitologia , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Coelhos , Schistosoma japonicum/crescimento & desenvolvimento , Esquistossomose Japônica/complicações , Esquistossomose Japônica/genética , Esquistossomose Japônica/parasitologia , Transdução de Sinais , Estômago/irrigação sanguínea , Estômago/efeitos dos fármacos , Estômago/patologiaRESUMO
A 62-year-old Brazilian man who lived in endemic areas of tropical diseases had an episode of hematemesis associated with portal hypertension. He used to swim in natural ponds during childhood and developed the hepatosplenic form of schistossomiasis with moderate ascites, in addition to the characteristic features of abdominal Caput Medusae. The aim of the report is highlight the role of chronic liver disease and schistossomiasis.
Assuntos
Varizes Esofágicas e Gástricas/parasitologia , Esquistossomose mansoni/diagnóstico , Varizes Esofágicas e Gástricas/diagnóstico , Humanos , Hipertensão Portal/complicações , Hipertensão Portal/diagnóstico , Hipertensão Portal/parasitologia , Masculino , Pessoa de Meia-Idade , Esquistossomose mansoni/complicaçõesRESUMO
BACKGROUND: Schistosomiasis is endemic to several parts of the world. Among the species that affect humans, Schistosoma mansoni is one of the most common causes of illness. In regions where schistosomiasis mansoni is endemic, reinfection is responsible for the emergence of hepatosplenic schistosomiasis (HSS) with portal hypertension in about 10% of infected individuals. Regardless of its etiology, portal hypertension may bring about the formation of arteriovenous fistulas and pulmonary vascular dilation, thus constituting a pulmonary shunt and its presence has been associated with the occurrence of neurological complications. The objective of this study was to identify pulmonary shunt using TTCE in patients with HSS and esophageal varices, and to compare the abdominal ultrasound and endoscopy findings among patients with and without pulmonary shunt. METHODOLOGY/PRINCIPAL FINDINGS: In this case series, a total of 461 patients with schistosomiasis mansoni were prospectively evaluated using abdominal ultrasound and endoscopy and 71 presented with HSS with esophageal varices. Fifty seven patients remained in the final analysis. The mean age of the patients was 55 ± 14 years, and 65% were female. Pulmonary shunts were observed in 19 (33.3%) patients. On comparing the groups with and without pulmonary shunt, no significant differences were observed in relation to the abdominal ultrasound and endoscopic findings. When comparing the two subgroups with pulmonary shunts (grade 1 vs grades 2 and 3), it was observed that the subgroup with shunt grades 2 and 3 presented with a significantly higher frequency of an enlarged splenic vein diameter (>0.9 cm), and an advanced pattern of periportal hepatic fibrosis (P = 0.041 and P = 0.005, respectively). None of the patients with pulmonary shunts had severe neurological complications. CONCLUSIONS/SIGNIFICANCE: Our findings suggest that in HSS with esophageal varices the pulmonary shunts may be present in higher grades and that in this condition it was associated with ultrasound findings compatible with advanced HSS.
Assuntos
Varizes Esofágicas e Gástricas/diagnóstico por imagem , Hipertensão Portal/diagnóstico por imagem , Hepatopatias Parasitárias/diagnóstico por imagem , Schistosoma mansoni/isolamento & purificação , Esquistossomose mansoni/diagnóstico por imagem , Esplenopatias/diagnóstico por imagem , Abdome/diagnóstico por imagem , Adulto , Idoso , Animais , Ecocardiografia/métodos , Varizes Esofágicas e Gástricas/complicações , Varizes Esofágicas e Gástricas/parasitologia , Varizes Esofágicas e Gástricas/fisiopatologia , Feminino , Humanos , Hipertensão Portal/complicações , Hipertensão Portal/parasitologia , Hipertensão Portal/fisiopatologia , Hepatopatias Parasitárias/complicações , Hepatopatias Parasitárias/parasitologia , Hepatopatias Parasitárias/fisiopatologia , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Esquistossomose mansoni/complicações , Esquistossomose mansoni/fisiopatologia , Esplenopatias/complicações , Esplenopatias/parasitologia , Esplenopatias/fisiopatologia , UltrassonografiaRESUMO
BACKGROUND: Hepatosplenic schistosomiasis (HES) has not been evaluated by transient elastography so far and its correlation with ultrasound variables remains to be defined. AIMS: The aim of this study was to describe the parameters of liver and spleen stiffness in HES assessed by transient elastography in comparison with cirrhotics and controls evaluating its correlation with ultrasonographic data. PATIENTS AND METHODS: HES, hepatitis C virus-cirrhotic, and control patients were included in this sectional study. Liver and spleen stiffness were compared among the three groups. The ultrasonographic parameters were compared with transient elastography in HES patients. RESULTS: Thirty HES, 30 hepatitis C virus-cirrhotic patients, and 17 controls were included. Those with HES presented liver stiffness that was significantly higher than the controls and lower than the cirrhotics: 9.7 (3.6-75.0) versus 3.7 (2.8-5.4) versus 27.0 (14.7-61.5) kPa (P<0.001). Spleen stiffness values were comparable between hepatosplenic and cirrhotics: 66.4 (25.7-75.0) versus 69.1 (18.0-75.0) kPa (P=0.78) and were significantly higher than the controls 16.5 kPa (6.3-34.3) (P<0.001). In patients with HES, high spleen stiffness was associated with right liver lobe diameter (P=0.015), splenic artery resistance index (P=0.002), portal vein diameter (P=0.021), portal vein area (P=0.008), portal vein congestion index (P=0.035), splenic vein diameter (P=0.013), and spleen diameter (P=0.021). CONCLUSION: Liver stiffness may be a useful tool to differentiate portal hypertension related to cirrhosis from that of HES. High spleen stiffness is a potential surrogate marker of portal hypertension in this population.
Assuntos
Técnicas de Imagem por Elasticidade , Hepatite C Crônica/diagnóstico por imagem , Hipertensão Portal/diagnóstico por imagem , Cirrose Hepática/diagnóstico por imagem , Fígado/diagnóstico por imagem , Esquistossomose/diagnóstico por imagem , Baço/diagnóstico por imagem , Ultrassonografia Doppler em Cores , Adulto , Idoso , Brasil , Estudos de Casos e Controles , Estudos Transversais , Diagnóstico Diferencial , Elasticidade , Feminino , Hepatite C Crônica/virologia , Humanos , Hipertensão Portal/parasitologia , Hipertensão Portal/virologia , Fígado/parasitologia , Fígado/virologia , Cirrose Hepática/patologia , Cirrose Hepática/virologia , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Esquistossomose/parasitologia , Baço/parasitologia , Baço/virologiaRESUMO
A 62-year-old Brazilian man who lived in endemic areas of tropical diseases had an episode of hematemesis associated with portal hypertension. He used to swim in natural ponds during childhood and developed the hepatosplenic form of schistossomiasis with moderate ascites, in addition to the characteristic features of abdominal Caput Medusae. The aim of the report is highlight the role of chronic liver disease and schistossomiasis
Un hombre natural de Brasil de 62 años de edad que vivía en zonas endémicas de enfermedades tropicales presentó un episodio de hematemesis asociada con hipertensión portal. Frecuentemente se bañaba en los estanques naturales durante la infancia y desarrolló la forma hepatosplénica de la esquistosomiasis con ascitis moderada, además de los rasgos abdominales característicos de la Cabeza de Medusa. El objetivo del informe es poner de relieve el papel de la enfermedad hepática crónica y de la esquistosomiasis
Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Esquistossomose mansoni/diagnóstico , Varizes Esofágicas e Gástricas/parasitologia , Esquistossomose mansoni/complicações , Varizes Esofágicas e Gástricas/diagnóstico , Hipertensão Portal/complicações , Hipertensão Portal/diagnóstico , Hipertensão Portal/parasitologiaRESUMO
Schistosomiasis is a major cause of fibrosis and portal hypertension. The reason 4-10% of infected subjects develops hepatosplenic schistosomiasis remains unclear. Chronically infected male CBA/J mice reproduce the dichotomic forms of human schistosomiasis. Most mice (80%) develop moderate splenomegaly syndrome (similar to hepatointestinal disease in humans) and 20% present severe hypersplenomegaly syndrome (analogous to human hepatosplenic disease). We demonstrated that the profibrogenic molecule osteopontin discriminates between mice with severe and mild disease and could be a novel morbidity biomarker in murine and human schistosomiasis. Failure to downregulate osteopontin during the chronic phase may explain why hepatosplenic subjects develop severe fibrosis.
Assuntos
Hipertensão Portal/diagnóstico , Enteropatias Parasitárias/diagnóstico , Cirrose Hepática/diagnóstico , Osteopontina/sangue , Esquistossomose mansoni/complicações , Esplenomegalia/diagnóstico , Análise de Variância , Animais , Biomarcadores/sangue , Modelos Animais de Doenças , Fibrose/sangue , Fibrose/diagnóstico , Fibrose/parasitologia , Humanos , Hipertensão Portal/sangue , Hipertensão Portal/parasitologia , Enteropatias Parasitárias/sangue , Enteropatias Parasitárias/parasitologia , Cirrose Hepática/sangue , Cirrose Hepática/parasitologia , Estudos Longitudinais , Masculino , Camundongos , Camundongos Endogâmicos CBA , Curva ROC , Esplenomegalia/parasitologiaRESUMO
This is a case report of a 24-year-old Ethiopian woman with a medical history of hepatosplenic schistosomiasis. She suffers from chronic liver failure and portal hypertension. She has been hospitalised for 'hysteria' in the past but did not receive follow-up, outpatient treatment or psychiatric evaluation. After discontinuing her medications and leaving her family to use holy water, a religious medicine used by many Ethiopians, she was found at a nearby monastery. She was non-communicative and difficult to arouse. The patient was rushed to nearby University of Gondar Hospital where she received treatment for hepatic encephalopathy and spontaneous bacterial peritonitis. Her illness is the result of neglected tropical disease, reliance on traditional medicine as opposed to biomedical services and the poor state of psychiatric care in the developing world.
Assuntos
Países em Desenvolvimento , Encefalopatia Hepática/parasitologia , Hepatopatias Parasitárias/complicações , Medicinas Tradicionais Africanas/efeitos adversos , Esquistossomose/complicações , Esplenopatias/complicações , Doença Hepática Terminal/parasitologia , Etiópia , Feminino , Humanos , Hipertensão Portal/parasitologia , Histeria/parasitologia , Hepatopatias Parasitárias/parasitologia , Hepatopatias Parasitárias/psicologia , Hepatopatias Parasitárias/terapia , Medicinas Tradicionais Africanas/métodos , Peritonite/microbiologia , Esquistossomose/psicologia , Esquistossomose/terapia , Esplenopatias/parasitologia , Esplenopatias/terapia , Adulto JovemRESUMO
UNLABELLED: Background. Upper gastrointestinal bleeding is a major cause of morbidity and mortality in patients with portal hypertension secondary to schistosomiasis mansoni. AIM: To evaluate the efficacy of combined surgery and sclerotherapy versus endoscopic treatment alone in the prophylaxis of esophageal variceal rebleeding due to portal hypertension in schistosomiasis. MATERIAL AND METHODS: During a two-years period consecutive patients with schistosomiasis and a recent bleeding history were evaluated for prospective randomization. Absolute exclusion criteria were alcoholism or other liver diseases, whereas platelet count < 50,000/mm3, INR > 1.5 or presence of gastric varices were relative exclusion criteria. By random allocation 25 (group A) have received endoscopic sclerotherapy for esophageal varices alone and 22 (group B) combined treatment: esophagogastric devascularization with splenectomy followed by sclerotherapy. Interim analysis at 24 months has shown significant statistical differences between the groups and the randomization was halted. RESULTS: Mean age was 38.9 ± 15.4 years and 58.46% were male. Mean follow-up was 38.6 ± 20.1 months. Endoscopic comparison of the size of esophageal varices before and after treatment did not show significant differences among the two groups. Treatment efficacy was assessed by the rate of recurrent esophageal variceal bleeding, that was more common in group A- 9/25 patients (36.0%) vs. 2/22 (9.0%) in group B (p = 0.029). Other complications were odynophagia, dysphagia and esophageal ulcer in group A and ascites and portal vein thrombosis in the surgical group. CONCLUSION: In portal hypertension due to schistosomiasis, combined surgical and endoscopic treatment was more effective for the prevention of recurrent esophageal variceal bleeding.
Assuntos
Varizes Esofágicas e Gástricas/terapia , Hemorragia Gastrointestinal/terapia , Hemostase Endoscópica/métodos , Hipertensão Portal/terapia , Hepatopatias Parasitárias/parasitologia , Schistosoma mansoni/patogenicidade , Esquistossomose mansoni/parasitologia , Escleroterapia , Esplenectomia , Adulto , Animais , Brasil , Terapia Combinada , Varizes Esofágicas e Gástricas/diagnóstico , Varizes Esofágicas e Gástricas/parasitologia , Feminino , Hemorragia Gastrointestinal/diagnóstico , Hemorragia Gastrointestinal/parasitologia , Hemostase Endoscópica/efeitos adversos , Humanos , Hipertensão Portal/diagnóstico , Hipertensão Portal/parasitologia , Hepatopatias Parasitárias/complicações , Hepatopatias Parasitárias/diagnóstico , Masculino , Pessoa de Meia-Idade , Recidiva , Esquistossomose mansoni/complicações , Esquistossomose mansoni/diagnóstico , Esplenectomia/efeitos adversos , Fatores de Tempo , Resultado do Tratamento , Adulto JovemRESUMO
Schistosomiasis is a major cause of portal hypertension worldwide. It associates with portal fibrosis that develops during chronic infection. The mechanisms by which the pathogen evokes these host responses remain unclear. We evaluated the hypothesis that schistosome eggs release factors that directly stimulate liver cells to produce osteopontin (OPN), a pro-fibrogenic protein that stimulates hepatic stellate cells to become myofibroblasts. We also investigated the utility of OPN as a biomarker of fibrosis and/or severity of portal hypertension. Cultured cholangiocytes, Kupffer cells and hepatic stellate cells were treated with soluble egg antigen (SEA); OPN production was quantified by quantitative reverse transcriptase polymerase chain reaction (qRTPCR) and ELISA; cell proliferation was assessed by BrdU (5-bromo-2'-deoxyuridine). Mice were infected with Schistosoma mansoni for 6 or 16 weeks to cause early or advanced fibrosis. Liver OPN was evaluated by qRTPCR and immunohistochemistry (IHC) and correlated with liver fibrosis and serum OPN. Livers from patients with schistosomiasis mansoni (early fibrosis n=15; advanced fibrosis n=72) or healthy adults (n=22) were immunostained for OPN and fibrosis markers. Results were correlated with plasma OPN levels and splenic vein pressures. SEA-induced cholangiocyte proliferation and OPN secretion (P<0.001 compared with controls). Cholangiocytes were OPN (+) in Schistosoma-infected mice and humans. Liver and serum OPN levels correlated with fibrosis stage (mice: r=0.861; human r=0.672, P=0.0001) and myofibroblast accumulation (mice: r=0.800; human: r=0.761, P=0.0001). Numbers of OPN (+) bile ductules strongly correlated with splenic vein pressure (r=0.778; P=0.001). S. mansoni egg antigens stimulate cholangiocyte proliferation and OPN secretion. OPN levels in liver and blood correlate with fibrosis stage and portal hypertension severity.
Assuntos
Proliferação de Células , Hipertensão Portal/metabolismo , Cirrose Hepática/metabolismo , Osteopontina/metabolismo , Esquistossomose mansoni/metabolismo , Adolescente , Adulto , Animais , Antígenos de Helmintos/farmacologia , Ductos Biliares/citologia , Ductos Biliares/efeitos dos fármacos , Ductos Biliares/metabolismo , Linhagem Celular , Células Cultivadas , Feminino , Células Estreladas do Fígado/efeitos dos fármacos , Células Estreladas do Fígado/metabolismo , Interações Hospedeiro-Parasita , Humanos , Hipertensão Portal/genética , Hipertensão Portal/parasitologia , Imuno-Histoquímica , Células de Kupffer/efeitos dos fármacos , Células de Kupffer/metabolismo , Cirrose Hepática/genética , Cirrose Hepática/parasitologia , Masculino , Camundongos , Pessoa de Meia-Idade , Osteopontina/sangue , Osteopontina/genética , Ratos , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Schistosoma/fisiologia , Esquistossomose mansoni/genética , Esquistossomose mansoni/parasitologia , Adulto JovemRESUMO
We present a case of a patient with hypersplenism secondary to portal hypertension due to hepato-splenic schistosomiasis, which was accompanied by severe and refractory thrombocytopenia. We performed spleen ablation and measured the total spleen and ablated volumes with contrast-enhanced computed tomography and volumetry. No major complications occurred, thrombocytopenia was resolved, and platelet levels remained stable, which allowed for early treatment of the patient's underlying disease. Previous work has shown that splenic radiofrequency ablation is an attractive alternative treatment for hypersplenism induced by liver cirrhosis. We aimed to contribute to the currently sparse literature evaluating the role of radiofrequency ablation (RFA) in the management of hypersplenism. We conclude that splenic RFA appears to be a viable and promising option for the treatment of hypersplenism.
Assuntos
Ablação por Cateter , Hiperesplenismo/cirurgia , Hepatopatias Parasitárias/parasitologia , Esquistossomose mansoni/parasitologia , Trombocitopenia/cirurgia , Meios de Contraste , Humanos , Hiperesplenismo/diagnóstico , Hiperesplenismo/parasitologia , Hipertensão Portal/diagnóstico , Hipertensão Portal/parasitologia , Hepatopatias Parasitárias/complicações , Hepatopatias Parasitárias/diagnóstico , Masculino , Pessoa de Meia-Idade , Contagem de Plaquetas , Valor Preditivo dos Testes , Esquistossomose mansoni/complicações , Esquistossomose mansoni/diagnóstico , Trombocitopenia/diagnóstico , Trombocitopenia/parasitologia , Tomografia Computadorizada por Raios X , Resultado do TratamentoRESUMO
AIM: To propose a less invasive surgical treatment for schistosomal portal hypertension. METHODS: Ten consecutive patients with hepatosplenic schistosomiasis and portal hypertension with a history of upper gastrointestinal hemorrhage from esophageal varices rupture were evaluated in this study. Patients were subjected to a small supraumbilical laparotomy with the ligature of the splenic artery and left gastric vein. During the procedure, direct portal vein pressure before and after the ligatures was measured. Upper gastrointestinal endoscopy was performed at the 30(th) postoperative day, when esophageal varices diameter were measured and band ligature performed. During follow-up, other endoscopic procedures were performed according to endoscopy findings. RESULTS: There was no intra-operative mortality and all patients had confirmed histologic diagnoses of schistosomal portal hypertension. During the immediate postoperative period, two of the ten patients had complications, one characterized by a splenic infarction, and the other by an incision hematoma. Mean hospitalization time was 4.1 d (range: 2-7 d). Pre- and post-operative liver function tests did not show any significant changes. During endoscopy thirty days after surgery, a decrease in variceal diameters was observed in seven patients. During the follow-up period (57-72 mo), endoscopic therapy was performed and seven patients had their varices eradicated. Considering the late postoperative evaluation, nine patients had a decrease in variceal diameters. A mean of 3.9 endoscopic banding sessions were performed per patient. Two patients presented bleeding recurrence at the late postoperative period, which was controlled with endoscopic banding in one patient due to variceal rupture and presented as secondary to congestive gastropathy in the other patient. Both bleeding episodes were of minor degree with no hemodynamic consequences or need for blood transfusion. CONCLUSION: Ligature of the splenic artery and left gastric vein with supraumbilical laparotomy is a promising and less invasive method for treating presinusoidal schistosomiasis portal hypertension.
Assuntos
Endoscopia , Hipertensão Portal/cirurgia , Pressão na Veia Porta , Esquistossomose/parasitologia , Artéria Esplênica/cirurgia , Adulto , Idoso , Varizes Esofágicas e Gástricas/parasitologia , Feminino , Hemorragia Gastrointestinal/parasitologia , Humanos , Hipertensão Portal/diagnóstico , Hipertensão Portal/parasitologia , Hipertensão Portal/fisiopatologia , Tempo de Internação , Ligadura , Testes de Função Hepática , Masculino , Pessoa de Meia-Idade , Projetos Piloto , Complicações Pós-Operatórias/etiologia , Estudos Prospectivos , Esquistossomose/complicações , Esquistossomose/diagnóstico , Artéria Esplênica/fisiopatologia , Fatores de Tempo , Resultado do TratamentoRESUMO
We report for the first time in the Philippines a case of portal vein thrombosis in a 12 year old Filipino boy with advanced schistosomiasis. The boy was referred to the Research Institute for Tropical Medicine (RITM), Manila, due to a rapidly enlarging spleen post-praziquantel treatment. At RITM, liver function tests were within normal limits but complete blood examinations showed pancytopenia and abnormal coagulation times. Serum markers for hepatitis A, B and C were negative. Abdominal MRI revealed schistosome-induced periportal fibrosis. The main portal vein appeared thrombosed with characteristic cavernous transformation of the right portal vein. Varices were seen in the oesophagus, gastrohepatic ligament, and splenic hilum. The spleen was markedly enlarged, with parenchymal foci representing Gamna-Gandy bodies. The patient underwent splenectomy. Histopathologic findings in the liver showed moderate pipestem fibrosis and schistosome egg granulomas. The patient was discharged from the hospital in excellent clinical condition.
Assuntos
Veia Porta , Esquistossomose/complicações , Esplenectomia , Esplenomegalia/cirurgia , Trombose Venosa/parasitologia , Criança , Diagnóstico Diferencial , Humanos , Hipertensão Portal/parasitologia , Masculino , Esplenomegalia/diagnóstico , Esplenomegalia/parasitologia , Resultado do Tratamento , Trombose Venosa/diagnósticoRESUMO
AIM: To investigate the association between endogenous hydrogen sulfide (H2S) and portal hypertension as well as its effect on vascular smooth muscle cells. METHODS: Portal hypertension patients were categorized by Child-Pugh score based on bilirubin and albumin levels, prothrombin time, ascites and hepatic encephalopathy. Plasma H2S concentrations and portal vein diameters (PVDs) were compared between portal hypertension patients and control participants, as well as between portal hypertension patients with varying degrees of severity. In addition, we established a rabbit hepatic schistosomiasis portal hypertension (SPH) model and analyzed liver morphology, fibrosis grade, plasma and liver tissue H2S concentrations, as well as cystathionine γ-lyase (CSE) activity and phosphorylated extracellular signal-regulated kinase (pERK)1/2, B cell lymphoma (Bcl)-2 and Bcl-XL expression in portal vein smooth muscle cells, in addition to their H2S-induced apoptosis rates. RESULTS: In portal hypertension patients, endogenous H2S levels were significantly lower than those in healthy controls. The more severe the disease was, the lower were the H2S plasma levels, which were inversely correlated with PVD and Child-Pugh score. Liver tissue H2S concentrations and CSE expression were significantly lower in the SPH rabbit livers compared with the control animals, starting at 3 wk, whereas pERK 1/2 expressions gradually increased 12-20 wk after SPH model establishment. In portal vein smooth muscle cells, increasing H2S levels led to increased apoptosis, while Bcl-2 and Bcl-XL expression decreased. CONCLUSION: H2S prevents vascular restructuring caused by excessive proliferation of smooth muscle cells via apoptosis induction, which helps to maintain normal vascular structures.
Assuntos
Junção Esofagogástrica/irrigação sanguínea , Junção Esofagogástrica/metabolismo , Sulfeto de Hidrogênio/sangue , Hipertensão Portal/sangue , Fígado/metabolismo , Músculo Liso Vascular/metabolismo , Miócitos de Músculo Liso/metabolismo , Adulto , Animais , Apoptose , Estudos de Casos e Controles , Proliferação de Células , Células Cultivadas , Modelos Animais de Doenças , Feminino , Humanos , Hipertensão Portal/parasitologia , Hipertensão Portal/patologia , Fígado/patologia , Cirrose Hepática Experimental/metabolismo , Cirrose Hepática Experimental/parasitologia , Masculino , Pessoa de Meia-Idade , Músculo Liso Vascular/patologia , Miócitos de Músculo Liso/patologia , Veia Porta/metabolismo , Veia Porta/patologia , Coelhos , Esquistossomose/complicações , Índice de Gravidade de Doença , Fatores de TempoRESUMO
CONTEXT: Data on vascular alterations in patients with hepatosplenic schistosomiasis and portal hypertensive colopathy and changes in these after surgery to decrease portal hypertension are limited. OBJECTIVE: The purpose of this study was to analyse the alterations of portal hypertensive colopathy previously and 6-12 months after splenectomy and gastric devascularization. METHODS: Twelve patients with hepatosplenic schistosomiasis who also had upper gastrointestinal bleeding were studied prospectively. Their endoscopic findings before and 6-12 months after the surgery were analysed. In addition, mucosal biopsies from ascending colon, sigmoid colon and rectum at these time points were subjected to histological and histomorphometric assessment. It was used a control group due to lack of normal pattern of the histomorphometric measures of vessels in individuals without portal hypertension. The critical level of significance adopted in all tests was of a maximum probability error of 5%. RESULTS: Surgery did not lead to significant improvement in histological and endoscopic findings. However, on histomorphometry, there was a significant decrease in the area, diameter and thickness of the vessels in mucosa at all colonic sites. CONCLUSION: Surgery for decompression of schistosomal portal hypertension has a beneficial effect on the associated colopathy, being best indicated in patients with gastrointestinal bleeding and esophageal varices.
Assuntos
Doenças do Colo/parasitologia , Varizes Esofágicas e Gástricas/parasitologia , Hemorragia Gastrointestinal/parasitologia , Hipertensão Portal/parasitologia , Esquistossomose mansoni/complicações , Doenças do Colo/cirurgia , Colonoscopia , Varizes Esofágicas e Gástricas/cirurgia , Hemorragia Gastrointestinal/cirurgia , Humanos , Hipertensão Portal/cirurgia , Estudos Prospectivos , Esquistossomose mansoni/cirurgia , Índice de Gravidade de Doença , Esplenectomia , Resultado do TratamentoRESUMO
BACKGROUND: The schistosomiasis affects 200 million people in 70 countries worldwide. It is estimated that 10% of those infected will develop hepatosplenic status and of these, 30% will progress to portal hypertension and esophagogastric varices, whose expression is through gastrointestinal bleeding with significant mortality in the first bleeding episode. Multiple surgical techniques have been developed to prevent re-bleeding. AIM: To evaluate the evolutional profile of esophageal varices after splenectomy + ligation of the left gastric vein associated with endoscopic sclerotherapy in schistosomal portal hypertension. METHODS: Prospective and observational study including schistosomiasis patients with previous history of upper digestive hemorrhage and underwent to splenectomy + ligation of the left gastric vein and sclerotherapy. The variables were: evolutional profile of esophageal varices before and after surgery and re-bleeding rate. RESULTS: The sample included 30 patients, 15 patients for each gender. The age ranged from 19 to 74 years (median = 43 years). There was a reduction in the degree, caliber and red spots in all patients (p< 0.05). The eradication of varices with sclerotherapy was achieved in 86.7% and with surgery alone in 15.4%. The mean follow-up was 28 months, ranging from two to 76 months. Were carried from one to seven sessions of sclerotherapy and the average was three per patient to eradicate varices. Four (13.3%) did not complete the follow-up. The re-bleeding rate was 16.7%. CONCLUSION: There was a reduction of the degree, caliber and red spots of esophageal varices in all patients.