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1.
Methods Mol Biol ; 2100: 395-405, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31939138

RESUMO

Alginate has demonstrated high applicability as a matrix-forming biomaterial for cell immobilization due to its ability to make hydrogels combined with cells in a rapid and non-toxic manner in physiological conditions, while showing excellent biocompatibility, preserving immobilized cell viability and function. Moreover, depending on its application, alginate hydrogel physicochemical properties such as porosity, stiffness, gelation time, and injectability can be tuned. This technology has been applied to several cell types that are able to produce therapeutic factors. In particular, alginate has been the most commonly used material in pancreatic islet entrapment for type 1 diabetes mellitus treatment. This chapter compiles information regarding the alginate handling, and we describe the most important steps and recommendations to immobilize insulin-producing cells within a tuned injectable alginate hydrogel using a syringe-based mixing system, detailing how to assess the viability and the biological functionality of the embedded cells.


Assuntos
Alginatos , Materiais Biocompatíveis , Células Imobilizadas , Hidrogéis , Células Secretoras de Insulina , Alicerces Teciduais , Animais , Linhagem Celular , Sobrevivência Celular , Diabetes Mellitus Tipo 1/terapia , Insulinas/biossíntese , Engenharia Tecidual
2.
Biomed J ; 41(6): 337-339, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30709574

RESUMO

In this issue of the Biomedical Journal, we highlight original research that will help to guide the choice of insulin administration regimes for children with type 1 diabetes. We also investigate whether a common medication for attention deficit/hyperactivity disorder worsens sleep problems among these children, and discover a new approach to maximize the lifetime of a fragile piece of surgical equipment.


Assuntos
Diabetes Mellitus Tipo 1/tratamento farmacológico , Insulinas/uso terapêutico , Extratos Vegetais/uso terapêutico , Animais , Criança , Diabetes Mellitus Tipo 1/diagnóstico , Modelos Animais de Doenças , Humanos , Insulinas/biossíntese
3.
Sheng Wu Gong Cheng Xue Bao ; 33(7): 1178-1189, 2017 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-28869737

RESUMO

To improve the yield of insulin precursor (PI), we constructed a recombinant expression vector pPIC9K-PI and transformed it into Pichia pastoris GS115 using electroporation. After screening, a mutant strain CL012 with 12 copies was obtained on the YPDS plate containing 4.0 mg/mL G418. Then, the components of SNAREs (Soluble N-ethylmaleimide-sensitive factor attachment receptor proteins), SNC2 and SNC2-SSO2, were expressed in the strain CL012 to explore the effect of SNAREs on the yield of PI. In shake flask culture, the strains expressing SNC2 and SNC2-SSO2 yielded PI of 1.89 mg/L and 2.21 mg/L after methanol induction for 96 h, which were improved by 23.53% and 44.44% compared to that of strain CL012 (1.53 mg/L), respectively. Further, in a 5-L bioreactor, the yield of PI with strain CL012 was 53 mg/L for high-density fermentation, after 96 h of methanol induction, which was 34.64-fold higher than that of shake culture. The strains expressing SNC2 and SNC2-SSO2 yielded the PI of 64 mg/L and 78 mg/L, which were respectively increased by 20.75% and 47.17%, compared to that of strain CL012. This work indicated that SNAREs components promoted the secretion of PI to improve its heterologous expression in P. pastoris.


Assuntos
Reatores Biológicos , Insulinas/biossíntese , Pichia , Proteínas Recombinantes/biossíntese , Fermentação , Microbiologia Industrial
4.
PLoS Genet ; 12(12): e1006450, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27926939

RESUMO

Misfolded proteins in transgenic models of conformational diseases interfere with proteostasis machinery and compromise the function of many structurally and functionally unrelated metastable proteins. This collateral damage to cellular proteins has been termed 'bystander' mechanism. How a single misfolded protein overwhelms the proteostasis, and how broadly-expressed mutant proteins cause cell type-selective phenotypes in disease are open questions. We tested the gain-of-function mechanism of a R37C folding mutation in an endogenous IGF-like C.elegans protein DAF-28. DAF-28(R37C) is broadly expressed, but only causes dysfunction in one specific neuron, ASI, leading to a distinct developmental phenotype. We find that this phenotype is caused by selective disruption of normal biogenesis of an unrelated endogenous protein, DAF-7/TGF-ß. The combined deficiency of DAF-28 and DAF-7 biogenesis, but not of DAF-28 alone, explains the gain-of-function phenotype-deficient pro-growth signaling by the ASI neuron. Using functional, fluorescently-tagged protein, we find that, in animals with mutant DAF-28/IGF, the wild-type DAF-7/TGF-ß is mislocalized to and accumulates in the proximal axon of the ASI neuron. Activation of two different branches of the unfolded protein response can modulate both the developmental phenotype and DAF-7 mislocalization in DAF-28(R37C) animals, but appear to act through divergent mechanisms. Our finding that bystander targeting of TGF-ß explains the phenotype caused by a folding mutation in an IGF-like protein suggests that, in conformational diseases, bystander misfolding may specify the distinct phenotypes caused by different folding mutations.


Assuntos
Efeito Espectador/genética , Proteínas de Caenorhabditis elegans/genética , Insulinas/genética , Dobramento de Proteína , Fator de Crescimento Transformador beta/genética , Animais , Caenorhabditis elegans/genética , Caenorhabditis elegans/crescimento & desenvolvimento , Proteínas de Caenorhabditis elegans/biossíntese , Proteínas de Caenorhabditis elegans/química , Regulação da Expressão Gênica no Desenvolvimento , Insulinas/biossíntese , Insulinas/química , Larva/genética , Larva/crescimento & desenvolvimento , Mutação , Neurônios/metabolismo , Neurônios/patologia , Fenótipo , Receptor de Insulina/genética , Fator de Crescimento Transformador beta/biossíntese , Fator de Crescimento Transformador beta/química
5.
PLoS Genet ; 11(5): e1005209, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-26020940

RESUMO

The coordination of growth with nutritional status is essential for proper development and physiology. Nutritional information is mostly perceived by peripheral organs before being relayed to the brain, which modulates physiological responses. Hormonal signaling ensures this organ-to-organ communication, and the failure of endocrine regulation in humans can cause diseases including obesity and diabetes. In Drosophila melanogaster, the fat body (adipose tissue) has been suggested to play an important role in coupling growth with nutritional status. Here, we show that the peripheral tissue-derived peptide hormone CCHamide-2 (CCHa2) acts as a nutrient-dependent regulator of Drosophila insulin-like peptides (Dilps). A BAC-based transgenic reporter revealed strong expression of CCHa2 receptor (CCHa2-R) in insulin-producing cells (IPCs) in the brain. Calcium imaging of brain explants and IPC-specific CCHa2-R knockdown demonstrated that peripheral-tissue derived CCHa2 directly activates IPCs. Interestingly, genetic disruption of either CCHa2 or CCHa2-R caused almost identical defects in larval growth and developmental timing. Consistent with these phenotypes, the expression of dilp5, and the release of both Dilp2 and Dilp5, were severely reduced. Furthermore, transcription of CCHa2 is altered in response to nutritional levels, particularly of glucose. These findings demonstrate that CCHa2 and CCHa2-R form a direct link between peripheral tissues and the brain, and that this pathway is essential for the coordination of systemic growth with nutritional availability. A mammalian homologue of CCHa2-R, Bombesin receptor subtype-3 (Brs3), is an orphan receptor that is expressed in the islet ß-cells; however, the role of Brs3 in insulin regulation remains elusive. Our genetic approach in Drosophila melanogaster provides the first evidence, to our knowledge, that bombesin receptor signaling with its endogenous ligand promotes insulin production.


Assuntos
Proteínas de Drosophila/biossíntese , Proteínas de Drosophila/genética , Insulina/metabolismo , Insulinas/biossíntese , Neuropeptídeos/genética , Receptores da Bombesina/genética , Receptores Odorantes/genética , Animais , Animais Geneticamente Modificados , Encéfalo/metabolismo , Drosophila melanogaster , Corpo Adiposo/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Humanos , Células Secretoras de Insulina/metabolismo , Insulinas/genética , Neuropeptídeos/biossíntese , Receptores Odorantes/biossíntese
6.
Mol Biol Rep ; 41(9): 5891-902, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24969480

RESUMO

The aim of the present study was construction of mammary gland specific expression vector for high level of human insulin (hINS) expression in transgenic buffalo for therapeutic use. We have constructed mammary gland specific vector containing human insulin gene and there expression efficiency was checked into in vitro cultured buffalo mammary epithelial cells (BuMECs). Human pro-insulin coding region was isolated from human genomic DNA by intron skipping PCR primer and furin cleavage site was inserted between B-C and C-A chain of human insulin by overlap extension PCR. A mammary gland-specific buffalo beta-lactoglobulin promoter was isolated from buffalo DNA and used for human insulin expression in BuMEC cells. The construct was transfected into BuMECs by lipofection method and positive transgene cell clones were obtained by G418 selection after 3 weeks. Expression of hINS in transfected cells were confirmed by RT-PCR, Immunocytochemistry, Western Blotting and ELISA. The pAcISUBC insulin-expressing clones secreted insulin at varying levels between 0.18 - 1.43 ng/ml/24 h/2.0 × 10(6) cells.


Assuntos
Vetores Genéticos , Insulinas/biossíntese , Glândulas Mamárias Animais/citologia , Proteínas Recombinantes/biossíntese , Animais , Animais Geneticamente Modificados , Sequência de Bases , Búfalos/genética , Células Cultivadas , Clonagem Molecular , Células Epiteliais/citologia , Éxons , Feminino , Regulação da Expressão Gênica , Humanos , Lactoglobulinas/genética , Dados de Sequência Molecular , Regiões Promotoras Genéticas , Transfecção , Transgenes
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