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1.
Asian Pac J Cancer Prev ; 22(12): 3927-3932, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34967573

RESUMO

OBJECTIVE: Infection with human tumor viruses is one of the hypothesized causes of cancer. The current investigation aimed to explore the presence and quantitative analysis of a new human tumor virus, Merkel cell polyomavirus (MCPyV) in tissue samples of 114 patients with oral cavity lesions including oral squamous cell carcinoma (OSCC), oral lichen planus (OLP), Dysplasia and oral irritation fibroma (OIF) in Northern Iran. METHODS: From 114 formalin fixed paraffin embedded samples; 35 with SCC, 29 with OLP, 14 with dysplasia and 36 with OIF were cut, deparaffinized and DNA was extracted. Quantitative detection of MCPyV large T antigen was performed by absolute quantitative Real-Time PCR. RESULT: MCPyV DNA was detected in 30.6% (n: 11/36) of IF, 24.1% (n; 7/29) of OLP, 21.4% (n:3/14) of dysplasia and 20% (n;7/35) of OSCC samples. The mean MCPyV DNA copy number was 2.32×10-2 ± 3.97 ×10-2, 2.02×10-2 (SD=3.13×10-2), 2.69×10-4 (SD=2.51×10-4), and 2.56×10-4 (SD=6.73×10-4) per cell in OSCC, dysplasia and both of OLP and OIF samples, respectively (P=0.76). CONCLUSION: This study provides the first data from Iran regarding the presence of MCPyV genome in oral cavity lesions and oral cancer. These results also emphasize that MCPyV has an active role in the occurrence of oral lesions and progression to cancer. Further studies should be carried out to clarify the role of MCPyV in oral cavity lesions.


Assuntos
Poliomavírus das Células de Merkel/isolamento & purificação , Doenças da Boca/epidemiologia , Neoplasias Bucais/epidemiologia , Infecções por Polyomavirus/epidemiologia , Infecções Tumorais por Vírus/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Biópsia , Carcinoma de Célula de Merkel/epidemiologia , Carcinoma de Célula de Merkel/virologia , Criança , DNA Viral/análise , Feminino , Fibroma/epidemiologia , Fibroma/virologia , Neoplasias de Cabeça e Pescoço/epidemiologia , Neoplasias de Cabeça e Pescoço/virologia , Humanos , Irã (Geográfico)/epidemiologia , Líquen Plano Bucal/epidemiologia , Líquen Plano Bucal/virologia , Masculino , Poliomavírus das Células de Merkel/genética , Pessoa de Meia-Idade , Boca/virologia , Doenças da Boca/virologia , Neoplasias Bucais/virologia , Infecções por Polyomavirus/virologia , Prevalência , Reação em Cadeia da Polimerase em Tempo Real , Neoplasias Cutâneas/epidemiologia , Neoplasias Cutâneas/virologia , Carcinoma de Células Escamosas de Cabeça e Pescoço/epidemiologia , Carcinoma de Células Escamosas de Cabeça e Pescoço/virologia , Infecções Tumorais por Vírus/virologia , Adulto Jovem
2.
Asian Pac J Cancer Prev ; 22(6): 1875-1881, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-34181346

RESUMO

OBJECTIVES: The main objectives of this study were to investigate the detection rate of high-risk human papillomavirus types 16 and 18 (high-risk HPV16/18) in oral potentially malignant disorders (OPMDs) including oral leukoplakia (OL) and oral lichen planus (OLP) in a Thai population and their associations with demographic, risk habits, and clinicopathologic features. METHODS: Paraffin-embedded formalin-fixed specimens from 101 OL and 59 OLP patients with patients' demographic, risk habits, and clinicopathologic data were collected. Conventional qualitative polymerase chain reaction was used to detect high-risk HPV16/18 DNA. Associations between high-risk HPV type 16/18 and demographic, clinicopathologic, risk factors (tobacco and alcohol uses) of OPMDs were analysed by Chi-square or Fisher's exact test. The results with p value less than 0.05 were considered statistically significant. RESULTS: HPV16/18 DNA was found in both OL and OLP groups with the detection rate of 19.8% and 18.6%, respectively. Approximately 90% of high-risk HPV were HPV18 subtype. Additionally, in OL group, high-risk HPV was found more frequently in patients with moderate/severe dysplasia than that in mild dysplasia. Interestingly, in OLP group, high-risk HPV was only detected in atrophic/ulcerative subtypes. None of risk factors was associated with high-risk HPV. CONCLUSIONS: Approximately 19% of OPMDs were HPV16/18-positive. HPV18 DNA was predominantly detected in both OL and OLP patients (90%). Additionally, the detection rate of high-risk HPV was higher in more severe dysplastic cases of OL and more clinically severe cases of OLP.


Assuntos
Papillomavirus Humano 18/isolamento & purificação , Leucoplasia Oral/virologia , Líquen Plano Bucal/virologia , Infecções por Papillomavirus/virologia , Lesões Pré-Cancerosas/virologia , Adulto , Idoso , Feminino , Papillomavirus Humano 16/isolamento & purificação , Humanos , Leucoplasia Oral/epidemiologia , Líquen Plano Bucal/epidemiologia , Masculino , Pessoa de Meia-Idade , Infecções por Papillomavirus/epidemiologia , Lesões Pré-Cancerosas/epidemiologia , Prevalência , Fatores de Risco , Tailândia/epidemiologia
3.
Rom J Intern Med ; 59(4): 359-368, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-33951354

RESUMO

Introduction. Lichen planus (LP) is a mucocutaneous T-cell mediated disorder of unknown etiology. There is growing evidence that oxidative stress is an important player in the pathogenesis of LP. Therefore, we have investigated oxidative stress markers in LP and the influence of hepatitis C virus (HCV) infection, a frequently associated condition, on oxidative stress in LP patients. Method. We have determined the serum levels of 4- hydroxynonenal (4-HNE) and symmetric dimethylarginine (SDMA), as markers of oxidative stress, and total antioxidant capacity (TAC), as a marker of the antioxidant defence, in 4 groups: group A - HCV positive patients with LP (n=12), group B - HCV positive patients without LP (n=12), group C - HCV negative patients with LP (n=31) and group D - control group (n=26). Results. In LP patients, we have identified an increased level of lipid peroxidation (4-HNE - group A - 8.41±1.11 µg/mL, group B - 7.97±2.17 µg/mL, group C - 7.81±1.96 µg/mL and group D - 6.15±1.17 µg/mL) and alterations in arginine methylation (SDMA - group A - 1.10±0.24 µmol/L, group B - 1.03±0.16 µmol/L, group C - 0.84±0.19 µmol/L and group D - 0.50±0.06 µmol/L) associated with a diminished antioxidant defence (TAC - group A - 234.50±49.96, µmol/L group B - 255.83±41.41 µmol/L, group C - 269.83±43.33 µmol/L and group D - 316.46 ±29.33 µmol/L), processes augmented by the association with HCV infection. Conclusion. There is an imbalance between oxidants and antioxidants in patients with LP, an imbalance that is augmented by the presence of HCV infection. SDMA could be regarded as a novel biomarker of oxidative stress among these patients. To the best of our knowledge this is the first study to investigate the influence of HCV infection on oxidative stress in LP patients.


Assuntos
Aldeídos/sangue , Arginina/análogos & derivados , Hepacivirus , Hepatite C/complicações , Líquen Plano/complicações , Idoso , Antioxidantes , Arginina/sangue , Biomarcadores/sangue , Feminino , Hepacivirus/isolamento & purificação , Hepatite C/sangue , Humanos , Líquen Plano/sangue , Líquen Plano/virologia , Líquen Plano Bucal/sangue , Líquen Plano Bucal/virologia , Masculino , Pessoa de Meia-Idade , Estresse Oxidativo , Projetos Piloto , RNA Viral/sangue
4.
Eur Rev Med Pharmacol Sci ; 23(18): 8148-8157, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31599444

RESUMO

OBJECTIVE: The aim of this review is to describe the "hot points" of current clinical governance for oral HPV comprising the use of new diagnostic molecular procedures, namely, Pyrosequencing and Next Generation Sequencing. MATERIALS AND METHODS: The data on oral HPV was collected through two levels of research. First for all, we used the canonical medical search engines, PubMed, and Medline, followed by the study of current commercial tools for HPV diagnosis, particularly within commercial companies involved in the molecular procedures for HPV detecting and genotyping. RESULTS: Different medical procedures are now described and used throughout the world in HPV diagnosis and treatment. However, the laboratory methods are often validated and used for genital infections, and, in these cases, data are missing in the literature as regards the clinical approach for oral lesions. CONCLUSIONS: Dental care units are often the front line for a clinical evaluation of a possible HPV lesion in the oral cavity, which means that correct clinical governance could avoid a viral neoplastic progression of this disease with great advantages for the patient. In this case, the problem is due to the difficulty in lesion recognition but also and more especially the absence of correct laboratory diagnosis and subsequent treatment in the clinical course.


Assuntos
Doenças da Boca/diagnóstico , Doenças da Boca/terapia , Infecções por Papillomavirus/diagnóstico , Infecções por Papillomavirus/terapia , Carcinoma Verrucoso/diagnóstico , Carcinoma Verrucoso/terapia , Carcinoma Verrucoso/virologia , Criocirurgia , Hiperplasia Epitelial Focal/diagnóstico , Hiperplasia Epitelial Focal/terapia , Hiperplasia Epitelial Focal/virologia , Humanos , Terapia a Laser , Leucoplasia Oral/diagnóstico , Leucoplasia Oral/terapia , Leucoplasia Oral/virologia , Líquen Plano Bucal/diagnóstico , Líquen Plano Bucal/terapia , Líquen Plano Bucal/virologia , Doenças da Boca/virologia , Neoplasias Bucais/diagnóstico , Neoplasias Bucais/terapia , Neoplasias Bucais/virologia , Papiloma/diagnóstico , Papiloma/terapia , Papiloma/virologia , Papillomaviridae/genética , Infecções por Papillomavirus/virologia , Fotoquimioterapia , Carcinoma de Células Escamosas de Cabeça e Pescoço/diagnóstico , Carcinoma de Células Escamosas de Cabeça e Pescoço/terapia , Carcinoma de Células Escamosas de Cabeça e Pescoço/virologia , Verrugas/diagnóstico , Verrugas/terapia , Verrugas/virologia
6.
Oral Dis ; 25(4): 1100-1106, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30801844

RESUMO

OBJECTIVE: To verify whether there are differences between populations of CD8 + and FoxP3 + T cells in lesions of oral lichen planus associated with hepatitis C virus chronic infection (OLP-HCV) and lesions of idiopathic oral lichen planus (OLP-I). MATERIALS AND METHODS: A case-control study was performed using a convenience sample of 11 paraffin-embedded tissue blocks of OLP-HCV and 19 of OLP-I. Histological sections stained with haematoxylin and eosin were used to classify the intensity of inflammatory infiltrate. Immunohistochemistry was used to identify CD8 + and FoxP3 + T cells. The count of positive cells was compared between the two groups and correlated to clinical and demographic data (p < 0.05). RESULTS: There were no statistically significant differences in the distribution of CD8 + and FoxP3 + T cells regarding the inflammatory infiltrate in lesions of OLP-HCV and OLP-I. Atrophic/erosive lesions showed a higher relationship between counts of CD8/FoxP3 T cells per mm2 (p = 0.018) and counts of CD8 + T cells per mm2 (p = 0.034) in OLP-HCV group compared to OLP-I group. CONCLUSION: Overall, no difference was found between cell populations in the lesions of OLP-HCV and OLP-I. However, atrophic/erosive lesions of OLP-HCV had a higher amount of CD8 + T cells and lower FoxP3 expression.


Assuntos
Linfócitos T CD8-Positivos/patologia , Fatores de Transcrição Forkhead , Líquen Plano Bucal/metabolismo , Mucosa Bucal/patologia , Linfócitos T Reguladores/metabolismo , Linfócitos T Reguladores/patologia , Adulto , Idoso , Estudos de Casos e Controles , Hepatite C Crônica/complicações , Hepatite C Crônica/imunologia , Humanos , Inflamação/metabolismo , Inflamação/patologia , Líquen Plano Bucal/patologia , Líquen Plano Bucal/virologia , Pessoa de Meia-Idade
7.
PLoS One ; 14(1): e0210070, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30650110

RESUMO

BACKGROUND: Human papillomavirus (HPV) is the cause of a fraction of head and neck squamous cell carcinoma. Although this relation is well-known, it is still not clear the role of HPV in premalignant oral lesions such as oral lichen planus (OLP) and dysplasia. We aimed to evaluate the HPV-DNA prevalence and type distribution in a set of oral biopsies obtained from patients diagnosed with OLP and dysplasia, as well as the role of HPV in these lesions. METHODS: A retrospective cohort of all premalignant oral lesions consecutively diagnosed from March 30th 1995 to May 21st 2014 at Hospital of Bellvitge and Odontological University Hospital of Bellvitge was identified and classified in four groups: OLP (groups 1 and 2) and dysplasias (groups 3 and 4) that progressed or not to invasive cancer during follow-up. A random selection targeting 25 cases was aimed to be performed for each group. All selected cases were subjected to pathological evaluation, DNA quality control and HPV-DNA detection. HPV-DNA positive samples were further subject to p16INK4a analysis. RESULTS: A total of 83 cases yielded a valid HPV-DNA result. From those, 7 and 34 cases were OLP that progressed or not to invasive cancer during follow-up, whereas 24 and 18 cases were displasias that progressed or not to invasive cancer during follow-up, respectively. HPV-DNA was detected in 4 samples (3 dysplastic lesions and 1 OLP). Two samples were HPV16 positive (2%), 1 sample HPV18 positive (1%) and 1 sample (1%) was HPV indeterminate. Two out of four HPV-DNA positive cases had high p16INK4a expression and none of the HPV positive cases progressed to invasive cancer during long-term follow-up. CONCLUSIONS: We found a low HPV-DNA attributable fraction in premalignant lesions of the oral cavity, suggesting that HPV is unlikely to play a significant role in oral carcinogenesis in our setting.


Assuntos
Líquen Plano Bucal/patologia , Boca/patologia , Infecções por Papillomavirus/patologia , Lesões Pré-Cancerosas/patologia , Idoso , Carcinogênese , DNA Viral/análise , DNA Viral/genética , Feminino , Humanos , Líquen Plano Bucal/virologia , Masculino , Pessoa de Meia-Idade , Boca/virologia , Neoplasias Bucais/diagnóstico , Neoplasias Bucais/virologia , Papillomaviridae/classificação , Papillomaviridae/genética , Papillomaviridae/fisiologia , Infecções por Papillomavirus/virologia , Lesões Pré-Cancerosas/virologia , Estudos Retrospectivos , Espanha
8.
Arch Oral Biol ; 96: 189-194, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30290294

RESUMO

OBJECTIVES: This study aimed to determine the association of rs2430561 and rs4073 polymorphisms in the Interferon gamma (IFN-ɤ) and Interleukin 8 (IL-8) genes, respectively, with hepatitis C virus-related oral lichen planus and disease severity. DESIGN: This is a case-control study. 60 subjects were equally divided into patients with and without oral lichen planus. They were further subdivided into hepatitis C virus seropositive and seronegative patients. All patients were genotyped for IFN-γ rs2430561 thymine to adenine (T > A) and IL-8 rs4073 adenine to thymine (A > T) polymorphisms. All patients with oral lichen planus had their lesions measured and documented using the Escudier scoring system. RESULTS: Disease activity was significantly higher in the "oral lichen planus/hepatitis C virus-positive" patients than in the "oral lichen planus/hepatitis C virus-negative" patients (P = 0.003). IFN-γ rs2430561 T > A and IL-8 rs4073 A > T genotypes and allele frequencies were not associated with the oral lichen planus group or the normal group. Stratification of the two groups into HCV and non-HCV-infected patients or into erosive and non-erosive types revealed no significant associations. The "A-allele-containing" genotypes of IL-8 rs4073 A > T were significantly more prevalent in the patients with oral lichen planus than in those without. CONCLUSION: Hepatitis C virus infection is associated with the development of erosive oral lichen planus. The A-allele of IL-8 rs4073 A > T may have a role in the development and progression of oral lichen planus.


Assuntos
Hepatite C/complicações , Interferon gama/genética , Interleucina-8/genética , Líquen Plano Bucal/virologia , Polimorfismo de Nucleotídeo Único , Estudos de Casos e Controles , Progressão da Doença , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase em Tempo Real
9.
Bosn J Basic Med Sci ; 18(3): 217-223, 2018 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-29984679

RESUMO

Oral lichen planus (OLP) is a chronic inflammatory disease characterized by the occurrence of multiple, symmetrical lesions in the oral cavity. Hepatitis C virus (HCV) infection has been suggested as an etiological factor in OLP. The purpose of this review was to summarize the current literature regarding the treatment of OLP in patients with HCV infection. An electronic search of the PubMed database was conducted until January 2018, using the following keywords: OLP, HCV, corticosteroids, retinoids, immunomodulatory agents, surgical interventions, photochemotherapy, laser therapy, interferon, ribavirin, and direct-acting antivirals. We selected the articles focusing on the clinical features and treatment management of OLP in patients with/without HCV infection. Topical corticosteroids are considered the first-line treatment in OLP. Calcineurin inhibitors or retinoids can be beneficial for recalcitrant OLP lesions. Systemic therapy should be used in the case of extensive and refractory lesions that involve extraoral sites. Surgical intervention is recommended for isolated lesions. In patients with HCV, monotherapy with interferon (IFN)-α may either improve, aggravate or trigger OLP lesions, while combined IFN-α and ribavirin therapy does not significantly influence the progression of lesions. Direct-acting antiviral (DAA) therapy appears to be a promising approach in patients with HCV-related OLP, as it can improve symptoms of both liver disease and OLP, with fewer side effects. Nevertheless, for clinical utility of DAAs in OLP patients, further studies with larger sample sizes, adequate treatment duration, and long term follow-up are required.


Assuntos
Antivirais/uso terapêutico , Hepatite C/complicações , Interferon-alfa/efeitos adversos , Interferon-alfa/uso terapêutico , Líquen Plano Bucal/complicações , Ribavirina/uso terapêutico , Adulto , Idoso , Feminino , Humanos , Inflamação , Líquen Plano Bucal/tratamento farmacológico , Líquen Plano Bucal/virologia , Hepatopatias/complicações , Hepatopatias/tratamento farmacológico , Hepatopatias/virologia , Masculino , Pessoa de Meia-Idade , Prevalência
10.
J Dent Res ; 97(13): 1494-1500, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30004820

RESUMO

Epstein-Barr virus (EBV), in addition to its transforming properties, contributes to the pathogenesis of several inflammatory diseases. Here, we investigated its involvement in oral lichen planus (OLP), a common autoimmune-like disease of unknown etiopathogenesis that can display a malignant potential. EBV-infected cells (EBV+ cells) were sought in a large series of clinically representative OLPs ( n = 99) through in situ hybridization to detect small noncoding EBV-encoded RNAs. Overall, our results demonstrated that EBV was commonly found in OLP (74%), with significantly higher frequency (83%) in the erosive form than in the reticular/keratinized type mild form (58%). Strikingly, many erosive OLPs were massively infiltrated by large numbers of EBV+ cells, which could represent a large part of the inflammatory infiltrate. Moreover, the number of EBV+ cells in each OLP section significantly correlated with local inflammatory parameters (OLP activity, infiltrate depth, infiltrate density), suggesting a direct relationship between EBV infection and inflammatory status. Finally, we characterized the nature of the infiltrated EBV+ cells by performing detailed immunohistochemistry profiles ( n = 21). Surprisingly, nearly all EBV+ cells detected in OLP lesions were CD138+ plasma cells (PCs) and more rarely CD20+ B cells. The presence of EBV+ PCs in erosive OLP was associated with profound changes in cytokine expression profile; notably, the expression of key inflammatory factors, such as IL1-ß and IL8, were specifically increased in OLP heavily infiltrated with EBV+ PCs. Moreover, electron microscopy-based experiments showed that EBV+ PCs actively produced EBV viral particles, suggesting possible amplification of EBV infection within the lesion. Our study thus brings conclusive evidence showing that OLP is commonly infiltrated with EBV+ PCs, adding a further puzzling element to OLP pathogenesis, given that PCs are now considered to be major regulatory immune cells involved in several autoimmune diseases (ClinicalTrials.gov NCT02276573).


Assuntos
Herpesvirus Humano 4 , Líquen Plano Bucal/virologia , Plasmócitos/virologia , Adulto , Biópsia , Estudos de Casos e Controles , Citocinas/metabolismo , Feminino , França , Interações Hospedeiro-Patógeno , Humanos , Técnicas Imunoenzimáticas , Hibridização In Situ , Masculino , Microscopia Eletrônica , Estudos Retrospectivos
11.
Asian Pac J Cancer Prev ; 19(6): 1633-1637, 2018 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-29936791

RESUMO

Background: Oral lichen planus (OLP) is a chronic autoimmune disease with an unknown etiology. Dentists are usually the first medical practitioners to diagnose this condition although it also affects body parts other than the oral mucosa. Several studies have reported an association between the OLP and hepatitis B and C infections. This study aimed to determine the prevalence of hepatitis B virus (HBV) antigen and hepatitis C virus (HCV) antibodies in patients with OLP compared with healthy controls. Methods: In this case‒control study, 50 patients with clinical and histopathological characteristics of OLP, and 50 age- and sex-matched healthy controls supplied serum samples (5 mL) for evaluation by ELISA. Data were analyzed using SPSS Software, version 21. Chi-square test was applied as appropriate. Results: In this study, the 50 patients with OLP (33 females and 17 males) had a mean age of 42.0 ± 14.5 years, and the 50 healthy subjects (33 females and 17 males) a mean age of 41.9 ± 13.7 years. None demonstrated any evidence of HBV antigen or HCV antibodies. Discussion: We could not detect any association between OLP and viral hepatitis. This could be attributed to a lower prevalence of hepatitis viruses compared to other countries or genotypic variation or other etiological factors contributing in our cases.


Assuntos
Hepacivirus/isolamento & purificação , Vírus da Hepatite B/isolamento & purificação , Hepatite B/epidemiologia , Hepatite C/epidemiologia , Líquen Plano Bucal/epidemiologia , Mucosa Bucal/virologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos de Casos e Controles , Feminino , Seguimentos , Hepatite B/complicações , Hepatite B/virologia , Hepatite C/complicações , Hepatite C/virologia , Anticorpos Anti-Hepatite C/sangue , Humanos , Irã (Geográfico)/epidemiologia , Líquen Plano Bucal/complicações , Líquen Plano Bucal/virologia , Masculino , Pessoa de Meia-Idade , Prevalência , Prognóstico , Adulto Jovem
12.
Mol Oral Microbiol ; 33(1): 22-28, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-28869787

RESUMO

Oral lichen planus (OLP) is a variant of lichen planus (LP), a common chronic mucocutaneous inflammatory disease. Cutaneous lesions of LP are self-limiting, but OLP lesions are non-remissive, alternating periods of exacerbation and quiescence, and only symptomatic treatments exist for OLP. The precise etiology and pathogenesis of OLP are hardly understood, which is a major obstacle to the development of new therapeutics for this disease. OLP is considered a T-cell-mediated inflammatory disease. Although various antigens have been considered, what actually triggers the inflammatory response of T cells is unknown. Suggested predisposing factors include genetic factors, stress, trauma, and infection. The aim of this review was to determine whether microbial infection can cause OLP. We first reviewed the association between OLP and microbial factors, including viral, fungal, and bacterial infections. In addition, each microbial factor associated with OLP was assessed by modified guidelines of Fredricks and Relman to determine whether it establishes a causal relationship. In conclusion, no microbial factor yet fulfills the guidelines to establish the causality of OLP. By focusing on the unclarified issues, however, the potential roles of microbial factors in the pathogenesis of OLP will be soon elucidated.


Assuntos
Infecções/complicações , Líquen Plano Bucal/etiologia , Micoses/complicações , Viroses/complicações , Humanos , Líquen Plano Bucal/microbiologia , Líquen Plano Bucal/virologia , Linfócitos T
13.
J Transl Med ; 15(1): 133, 2017 06 07.
Artigo em Inglês | MEDLINE | ID: mdl-28592251

RESUMO

BACKGROUND: Oral lichen planus (OLP) is a T cell-mediated autoimmune disease. The aetiology and molecular mechanisms of OLP remain unclear. Human cytomegalovirus (HCMV) infection is a causal factor in the development of various diseases, but the clinical relevance of HCMV in OLP has not been thoroughly investigated. METHODS: In the present study, we firstly examined twenty-three HCMV-encoded microRNA (miRNA) expression profiles in plasma from training set that including 21 OLP patients and 18 healthy controls using RT-qPCR technology. Dysregulated miRNAs were subsequently confirmed in another larger cohort refereed as validation set consisting of 40 OLP patients and 33 healthy controls. HCMV DNA in peripheral blood leukocytes (PBLs) was also measured in an additional cohort of 13 OLP patients and 12 control subjects. Furthermore, bioinformatics analyses, luciferase reporter assay and western blotting were also performed to predict and verify the direct potential targets of HCMV-encoded miRNAs. RESULTS: The RT-qPCR results showed that the plasma levels of five HCMV-encoded miRNAs including hcmv-miR-UL112-3p, hcmv-miR-UL22a-5p, hcmv-miR-UL148d, hcmv-miR-UL36-5p and hcmv-miR-UL59 were significantly increased in OLP patients in both training and validation sets. HCMV DNA in PBLs was also significantly higher in OLP patients than in control subjects. Additionally, by using a combination of luciferase reporter assay and western blotting, we demonstrated that cytomegalovirus UL16-binding protein 1, a molecule that mediates the killing of virus-infected cells by natural killer cells, is a direct target of hcmv-miR-UL59. CONCLUSIONS: Our results demonstrate a distinct expression pattern of HCMV-encoded miRNAs in OLP patients, which may provide insight into the relationship between HCMV infection and OLP, and warrants additional study in the diagnosis and aetiology of OLP.


Assuntos
Perfilação da Expressão Gênica , Líquen Plano Bucal/genética , Líquen Plano Bucal/virologia , MicroRNAs/genética , Sequência de Bases , Estudos de Casos e Controles , Citomegalovirus , Demografia , Exossomos/metabolismo , Feminino , Proteínas Ligadas por GPI/metabolismo , Células HEK293 , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Líquen Plano Bucal/sangue , Masculino , MicroRNAs/metabolismo , Pessoa de Meia-Idade , Reprodutibilidade dos Testes , Regulação para Cima/genética
14.
J Med Virol ; 89(11): 2055-2057, 2017 11.
Artigo em Inglês | MEDLINE | ID: mdl-28661003

RESUMO

Oral lichen planus (OLP) is a chronic inflammatory disease considered as a CD8+ T lymphocyte-mediated autoimmune reaction, which may be triggered by undetermined virus. Recent reports have described the detection of Merkel cell polyomavirus (MCPyV) DNA in oral samples from healthy patients and in patients with different forms of oral cancers. We therefore investigated in a prospective way whether MCPyV was detectable in oral lesions of patients with active OLP. Our preliminary results do not support the hypothesis that OLP may be triggered by MCPyV infection. Further studies are needed to evaluate the involvement of other human polyomaviruses in OLP pathogenesis.


Assuntos
Líquen Plano Bucal/virologia , Poliomavírus das Células de Merkel/genética , Poliomavírus das Células de Merkel/isolamento & purificação , Boca/virologia , Infecções por Polyomavirus/virologia , Adulto , Idoso , DNA Viral/isolamento & purificação , Feminino , Humanos , Líquen Plano Bucal/complicações , Masculino , Pessoa de Meia-Idade , Boca/patologia , Neoplasias Bucais/virologia , Infecções por Polyomavirus/complicações , Estudos Prospectivos , Adulto Jovem
15.
BMJ Case Rep ; 20172017 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-28385698

RESUMO

Oral hairy leukoplakia (OHL) is an oral mucosal lesion that is associated with Epstein-Barr virus infection. It commonly presents as an asymptomatic, non-removable white patch on the lateral borders of the tongue in individuals who are immunocompromised. Historically, OHL was thought to be pathognomonic of HIV infection; however, it is now an established phenomenon in a range of conditions affecting immune competence. Hairy cell leukaemia (HCL) is a rare chronic B cell lymphoproliferative disease named after the distinctive cytology of the atypical cells. We report the first case of OHL arising in an individual with HCL that resolved following remission of the haematological malignancy.


Assuntos
Infecções por Vírus Epstein-Barr/complicações , Leucemia de Células Pilosas/tratamento farmacológico , Leucoplasia Pilosa/diagnóstico , Líquen Plano Bucal/diagnóstico , Humanos , Leucoplasia Pilosa/virologia , Líquen Plano Bucal/virologia , Masculino , Pessoa de Meia-Idade , Purinas
16.
PLoS One ; 11(8): e0161339, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27571417

RESUMO

BACKGROUND: The role of human papilloma virus (HPV) in oral lichen planus (OLP) is controversial. OBJECTIVES: The primary aim of the current study is to calculate the pooled risk estimates of HPV infection in OLP when compared with healthy controls. METHODS: Bibliographic searches were conducted in three electronic databases. Articles on the association between HPV and OLP were selected from case-control studies or cross-sectional studies, following predefined criteria. Pooled data were analyzed by calculating odds ratios (OR) and 95% confidence interval (CI). RESULTS: Of the 233 publications identified, 22 case-control studies met the inclusion criteria. Collectively, 835 cases and 734 controls were available for analysis. The summary estimate showed that OLP patients have significantly higher HPV prevalence (OR: 6.83; 95% CI: 4.15-11.27) than healthy controls. In subgroup analyses, the association of HPV and OLP varied significantly by geographic populations. The ORs ranged from 2.43 to 132.04. The correlation of HPV and erosive-atrophic oral lichen planus (EA-OLP) (OR: 9.34) was comparable and well above that of HPV and non-EA-OLP (OR: 4.32). Among HPV genotypes, HPV 16 showed an extremely strong association with OLP (OR: 11.27), and HPV 18 showed a relatively strong one (OR: 6.54). CONCLUSION: In conclusion, a significant association was found between HPV and OLP. The strength of the association varied across geographic populations, clinical types of OLP, and HPV genotypes. The results suggest that HPV might play an important causal role in OLP and in its malignant to progression.


Assuntos
Líquen Plano Bucal/virologia , Papillomaviridae/fisiologia , Estudos de Casos e Controles , Humanos
17.
Med Oral Patol Oral Cir Bucal ; 21(2): e161-8, 2016 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-26827059

RESUMO

BACKGROUND: China has been one of the countries with high prevalence of chronic hepatitis B virus (HBV) and hepatitis C virus (HCV) liver disease. And lichen planus is an extrahepatic manifestation of patients with chronic HCV infection. This case-control study was conducted to investigate the relationship between oral lichen planus (OLP) and HBV/HCV infection in China. MATERIAL AND METHODS: A total of 776 patients, including 150 patients with OLP (Group OLP), 429 inpatients from the Trauma Ward of Oral and Maxillofacial Surgery Department (Group A), 110 patients with other oral mucosal diseases, but without a reported association with HCV infection (Group B) and 87 patients with oral lichenoid lesion (Group OLL), were compared with their seroprevalence of anti-HCV antibody (HCVAb), hepatitis B surface antigen (HBsAg) and the parameters of liver functions. Moreover, the clinical characteristics of OLP were also observed, such as gender, age, chief complaint, course of the disease, clinical type, sites involved and so on. RESULTS: The positive rates of HCVAb and HBsAg in OLP patients were 0.7% and 4%, respectively. Neither HCVAb nor HBsAg was associated with OLP as demonstrated by both the univariate and the multivariate analyses. The clinical features and liver functions of OLP patients with negative or positive HBsAg were nearly the same. CONCLUSIONS: Our findings verify that there is no association between OLP and hepatitis and there is no need to run a screening test for HCV or HBV in OLP patients in China.


Assuntos
Antígenos da Hepatite B/sangue , Anticorpos Anti-Hepatite C/sangue , Líquen Plano Bucal/sangue , Adulto , Estudos de Casos e Controles , China , Feminino , Hepatite B Crônica/complicações , Hepatite C Crônica/complicações , Humanos , Líquen Plano Bucal/virologia , Masculino , Pessoa de Meia-Idade
18.
Beijing Da Xue Xue Bao Yi Xue Ban ; 48(1): 84-8, 2016 Feb 18.
Artigo em Chinês | MEDLINE | ID: mdl-26885914

RESUMO

OBJECTIVE: To investigate the possibility for human papillomavirus (HPV) infection to be a predictable signal for the carcinogenesis of oral mucosa by comparing the prevalences of HPV in each stage of oral mucosal carcinogenesis and to compare the sensitivity differences of the two methods in detecting HPV infection in oral cavity. METHODS: The hybrid capture (HC-II) was used to detect infection of HPV in 255 samples taken from 12 cases of healthy oral mucosa, 211 cases of patients with pathological diagnosis and 32 cases of patients with clinical diagnosis. The diagnosed cases included 8 cases of benign lesions of the oral mucosa, precancerous lesions [74 cases of oral leukoplakia (OLK) with hyperplasia and 42 cases of OLK with oral epithelial dysplasia (OED)], 91 cases of precancerous condition [oral lichen planus (OLP)] and 28 cases of oral squamous cell carcinoma (OSCC). And in situ hybridization (ISH) was used to detect infection of HPV in 33 cases of OSCC and 76 cases of OLK, including 30 cases of hyperplasia, 15 cases of mild OED, 15 cases of moderate OED and 16 cases of severe OED. RESULTS: The prevalence of HPV in OLP samples was higher (12.12%, 8/66) than that of OLK (2.59%, 3/116) (χ(2)=4.666, P=0.031) and OSCC(7.14%, 2/28, χ(2)=0.513, P=0.474). The prevalence of HPV in OSCC (7.14%, 2/28) was higher than that of OLK (2.59%, 3/116), and no significant difference was found. There was only one case of smoke spot and statistical analysis was not carried out. ISH was used to detect type 16/18 and type 31/33 HPV DNA in 109 cases of oral mucosal lesions in paraffin sections and only one case of OSCC was HPV positive. Thirty-seven cases were detected by HC-II and ISH methods at the same time. The same negative results by the two methods were found in 94.6% samples (35/37). In the other two samples, one was OSCC with early infiltration and the other was OLK with hyperplasia, The HC-II results were positive while the ISH results were negative. The patients with OLP and HPV testing results were followed up and the average follow-up period was (36.2 ± 10.5) months. It was found that three of them had a malignant transformation, and the malignant transformation rate of HPV positive patients was 12.50% (1/8), which was higher than that of HPV negative patients (3.45%, 2/58), and the difference was not statistically significant, P=0.249. CONCLUSION: HC-II assay was more sensitive in detecting HPV infection of oral mucosal lesions than ISH. The results of this study showed that there was insufficient evidence for taking HPV infection as a predictor of OLK carcinogenesis. Patients suffering from OLP were in a precancerous condition. The prevalence of HPV in OLP patients of this study was higher than that in OLK and OSCC patients, suggesting that for some reason, OLP patients were susceptible to HPV. HPV testing can be considered as routine in patients with OLP, and HC-II assay was recommended. And patients with OLP and HPV positive should be followed up regularly.


Assuntos
Carcinoma de Células Escamosas/virologia , Leucoplasia Oral/virologia , Líquen Plano Bucal/virologia , Neoplasias Bucais/virologia , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/patologia , Carcinogênese , Carcinoma de Células Escamosas/patologia , Transformação Celular Neoplásica , Humanos , Hibridização In Situ , Leucoplasia Oral/patologia , Líquen Plano Bucal/patologia , Mucosa Bucal/patologia , Neoplasias Bucais/patologia , Infecções por Papillomavirus/diagnóstico , Lesões Pré-Cancerosas/patologia , Lesões Pré-Cancerosas/virologia
19.
J Clin Pathol ; 69(4): 358-63, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26468392

RESUMO

AIMS: To detect the presence of Epstein-Barr virus (EBV) DNA in different sources of materials from a matched group of patients with oral lichen planus (OLP) and a group of people without OLP lesions, and to correlate the presence of virus with epidemiological variables of the groups studied. METHODS: Fresh tissue samples, saliva, exfoliated cells and plasma of 24 patients with OLP lesions (cases) and 17 patients without OLP lesions (controls) were collected. EBV was detected by nested PCR. RESULTS: Viral positivity was obtained in 62.5% of tissue samples; in 70.8% of exfoliated cell samples; in 33.3% of blood plasma samples and in 75% of saliva samples in the cases; and in 35.3% of tissue samples; 82.4% of exfoliated cell samples; in 47.1% of blood plasma samples and in 64.7% of saliva samples in the controls. There was a predominance of women in both groups. Variables not atrophic-erosive were most affected by EBV. CONCLUSIONS: No relationship between EBV and OLP was found. However, all sources tested in this study were considered suitable for the detection of viruses.


Assuntos
Infecções por Vírus Epstein-Barr/epidemiologia , Líquen Plano Bucal/virologia , Adulto , Idoso , Estudos de Casos e Controles , DNA Viral/análise , Feminino , Herpesvirus Humano 4 , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Prevalência
20.
Aust Dent J ; 61(3): 282-7, 2016 09.
Artigo em Inglês | MEDLINE | ID: mdl-26475515

RESUMO

BACKGROUND: A role for hepatitis C virus in oral lichen planus has been postulated. This systematic review and meta-analysis of the existing epidemiological studies was conducted to determine if there is a correlation between oral lichen planus and hepatitis C virus infection. METHODS: We examined the association between hepatitis C virus and oral lichen planus by conducting a systematic review and meta-analysis of case-control studies that examined the prevalence of anti-HCV antibodies in the serum of cases and controls. We searched PubMed, Embase and The Cochrane Library databases from 2005 to January 2015. Associations were measured using random-effect odds ratios (ORs) combined with 95% confidence intervals. RESULTS: Nineteen eligible studies, encompassing 1807 cases of OLP and 2519 controls, were retrieved and included in this review. The summary estimate OR for all studies was 6.07 (95% CI: 2.73-13.48), showing a statistically significant difference in the proportion of HCV seropositivity among oral lichen planus patients, compared with controls and substantial heterogeneity between studies (I(2) = 65%) as a result of a variety of geographical distributions. CONCLUSIONS: The association of hepatitis C virus infection with oral lichen planus emphasizes the importance of hepatitis C virus screening in oral lichen planus patients.


Assuntos
Hepatite C/epidemiologia , Líquen Plano Bucal/virologia , Hepatite C/complicações , Anticorpos Anti-Hepatite C/sangue , Humanos , Razão de Chances , Prevalência
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