RESUMO
In vertebrates, gonadotropin-releasing hormone (GnRH) peptide is the central mediator of reproduction. Homologous peptides have previously also been identified in molluscan species. However, emerging evidence suggests that these molecules might serve diverse regulatory functions and proposes to consider them as corazonin (CRZ). We previously isolated the full-length cDNA of the invGnRH/CRZ peptide (termed ly-GnRH/CRZ) in the well-established invertebrate model species, the great pond snail Lymnaea stagnalis; however, its predicted functions remain to be verified. In this study, we first confirmed the presence of the deduced active peptide from the central nervous system of L. stagnalis. Further, we performed in vivo and in vitro studies to explore the functions of ly-GnRH/CRZ. Injection of sexually mature specimens with synthetic active peptide had an inhibitory effect on locomotion and an acceleratory effect on egg-laying, but had no effect on feeding. The previously predicted modulatory effect of ly-GnRH/CRZ was supported by its identified co-localization with serotonin on the surface of the heart atria. Lastly, we demonstrated not only the presence of ly-GnRH/CRZ in the penial complex but also that ly-GnRH/CRZ-containing neurons project to the efferent penis nerve, suggesting ly-GnRH/CRZ may directly modulate the motor output of this peripheral tissue. Overall, our findings strongly support that ly-GnRH/CRZ is a multifunctional neuropeptide. These results contribute to the understanding of the GnRH superfamily and, more broadly, disciplines such as comparative endocrinology and neurobiology.
Assuntos
Lymnaea/fisiologia , Neuropeptídeos/fisiologia , Animais , Evolução Biológica , Sistema Nervoso Central/metabolismo , Comportamento Alimentar , Hormônio Liberador de Gonadotropina/química , Hormônio Liberador de Gonadotropina/fisiologia , Locomoção , Lymnaea/química , Neuropeptídeos/química , OviparidadeRESUMO
Lynx1, membrane-bound protein co-localized with the nicotinic acetylcholine receptors (nAChRs) and regulates their function, is a three-finger protein (TFP) made of three ß-structural loops, similarly to snake venom α-neurotoxin TFPs. Since the central loop II of α-neurotoxins is involved in binding to nAChRs, we have recently synthesized the fragments of Lynx1 central loop, including those with the disulfide between Cys residues introduced at N- and C-termini, some of them inhibiting muscle-type nAChR similarly to the whole-size water-soluble Lynx1 (ws-Lynx1). Literature shows that the main fragment interacting with TFPs is the C-loop of both nAChRs and acetylcholine binding proteins (AChBPs) while some ligand-binding capacity is preserved by analogs of this loop, for example, by high-affinity peptide HAP. Here we analyzed the structural organization of these peptide models of ligands and receptors and its role in binding. Thus, fragments of Lynx1 loop II, loop C from the Lymnaea stagnalis AChBP and HAP were synthesized in linear and Cys-cyclized forms and structurally (CD and NMR) and functionally (radioligand assay on Torpedo nAChR) characterized. Connecting the C- and N-termini by disulfide in the ws-Lynx1 fragment stabilized its conformation which became similar to the loop II within the 1H-NMR structure of ws-Lynx1, the activity being higher than for starting linear fragment but lower than for peptide with free cysteines. Introduced disulfides did not considerably change the structure of HAP and of loop C fragments, the former preserving high affinity for α-bungarotoxin, while, surprisingly, no binding was detected with loop C and its analogs.
Assuntos
Proteínas Adaptadoras de Transdução de Sinal/química , Bungarotoxinas/química , Proteínas de Transporte/ultraestrutura , Receptores Nicotínicos/química , Animais , Proteínas de Bactérias/química , Proteínas de Bactérias/ultraestrutura , Sítios de Ligação , Proteínas de Transporte/química , Humanos , Ligantes , Lymnaea/química , Lymnaea/genética , Modelos Moleculares , Neurotoxinas/química , Peptídeos/química , Ligação Proteica/genética , Conformação Proteica em Folha beta , Receptores Nicotínicos/ultraestruturaRESUMO
Ecological effects of gold nano-particles (AuNP) are examined due to growing use in consumer and industrial materials. This study investigated uptake and movement of AuNPs through an aquatic food chain. Simple (single-species) and diverse (multi-species) periphyton communities were exposed to AuNP (0, 100, 500 µg L-1 treatments). AuNP quickly aggregated and precipitated from the water column, suggesting it is an insignificant route of AuNP exposure even at elevated concentrations. Gold was measured in 100 and 500 µg L-1 periphyton treatments. Gold accumulation was similar between periphyton treatments, suggesting physical processes were important for AuNP basal accumulation. Hyalella azteca and Lymnea stagnalis whole body tissue analysis indicated gold accumulation may be attributed to different feeding mechanisms, general versus selective grazing, respectively. Results suggest trophic transfer of AuNP is organism specific and aggregation properties of AuNP are important when considering fate of nano-particles in the environment and movement through aquatic food webs.
Assuntos
Anfípodes/efeitos dos fármacos , Ouro/análise , Lymnaea/efeitos dos fármacos , Nanopartículas Metálicas/análise , Perifíton/efeitos dos fármacos , Poluentes Químicos da Água/análise , Anfípodes/química , Animais , Exposição Dietética , Cadeia Alimentar , Lymnaea/química , Especificidade da EspécieRESUMO
In the field of nicotinic acetylcholine receptors (nAChRs), recognized as important therapeutic targets, much effort has been dedicated to the development of nicotinic analogues to agonize or antagonize distinct homo- and heteropentamers nAChR subtypes, selectively. In this work we developed di- and heptavalent nicotinic derivatives based on ethylene glycol (EG) and cyclodextrin cores, respectively. The compounds showed a concentration dependent inhibition of acetylcholine-induced currents on α7 nAChR expressed by Xenopus oocytes. Interesting features were observed with the divalent nicotinic derivatives, acting as antagonists with varied inhibitory concentrations (IC50) in function of the spacer arm length. The best divalent compounds showed a 16-fold lowered IC50 compared to the monovalent reference (12 vs 195⯵M). Docking investigations provide guidelines to rationalize these experimental findings.
Assuntos
Antagonistas Nicotínicos/farmacologia , Polietilenoglicóis/farmacologia , Piridinas/farmacologia , Receptor Nicotínico de Acetilcolina alfa7/antagonistas & inibidores , beta-Ciclodextrinas/farmacologia , Animais , Feminino , Humanos , Ligantes , Lymnaea/química , Simulação de Acoplamento Molecular , Antagonistas Nicotínicos/síntese química , Antagonistas Nicotínicos/metabolismo , Oócitos/efeitos dos fármacos , Polietilenoglicóis/síntese química , Polietilenoglicóis/metabolismo , Ligação Proteica , Piridinas/síntese química , Piridinas/metabolismo , Xenopus laevis , Receptor Nicotínico de Acetilcolina alfa7/química , Receptor Nicotínico de Acetilcolina alfa7/metabolismo , beta-Ciclodextrinas/síntese química , beta-Ciclodextrinas/metabolismoRESUMO
Potentiation of the function of the α7 nicotinic acetylcholine receptor (α7-nAChR) is believed to provide a possible way for the treatment of cholinergic system dysfunctions such as Alzheimer's disease and schizophrenia. Positive allosteric modulators (PAMs) are able to augment the peak current response of the endogenous agonist of α7-nAChR by binding to some allosteric sites. In this study, the binding profile of a potent type I PAM, NS-1738, with a chimera structure (termed α7-AChBP) constructed from the extracellular domain of α7-nAChR and an acetylcholine binding protein was investigated with molecular docking, molecular dynamics simulation, and free energy calculation methods. We found that NS-1738 could bind to three allosteric sites of α7-AChBP, namely, the top pocket, the vestibule pocket and the agonist sub-pocket. NS-1738 has moderate binding affinities (-6.76 to -9.15 kcal mol-1) at each allosteric site. The urea group is critical for binding and can form hydrogen-bond interactions with the protein. The bulky trifluoromethyl group also has a great impact on the binding modes and binding affinities. We believe that our study provides valuable insight into the binding profiles of type I PAMs with α7-nAChR and is helpful for the development of novel PAMs.
Assuntos
Compostos de Fenilureia/química , Receptor Nicotínico de Acetilcolina alfa7/química , Sítio Alostérico , Animais , Humanos , Ligação de Hidrogênio , Lymnaea/química , Simulação de Acoplamento Molecular , Simulação de Dinâmica Molecular , Proteínas Recombinantes de Fusão/químicaRESUMO
Neurotransmitters are endogenous metabolites that allow the signal transmission across neuronal synapses. Their biological role is crucial for many physiological functions and their levels can be changed by several diseases. Because of their high polarity, hydrophilic interaction liquid chromatography (HILIC) is a promising tool for neurotransmitter analysis. Due to the large number of HILIC stationary phases available, an evaluation of the column performances and retention behaviors has been performed on five different commercial HILIC packing materials (silica, amino, amide and two zwitterionic stationary phases). Several parameters like the linear correlation between retention and the distribution coefficient (logD), the separation factor k and the column resolution Rs have been investigated and the column performances have been visualized with a heat map and hierarchical clustering analysis. An optimized and validated HILIC-MS/MS method based on the ZIC-cHILIC column is proposed for the simultaneous detection and quantification of twenty compounds consisting of neurotransmitters, precursors and metabolites: 3-methoxytyramine (3-MT), 5-hydroxyindoleacetic acid (5-HIAA), 5-hydroxy-L-tripthophan, acetylcholine, choline, L-3,4-dihydroxyphenylalanine (L-DOPA), dopamine, epinephrine, γ-aminobutyric acid (GABA), glutamate, glutamine, histamine, histidine, L-tryptophan, L-tyrosine, norepinephrine, normetanephrine, phenylalanine, serotonin and tyramine. The method was applied to neuronal metabolite profiling of the central nervous system of the freshwater snail Lymnaea stagnalis. This method is suitable to explore neuronal metabolism and its alteration in different biological matrices.
Assuntos
Técnicas de Química Analítica/métodos , Cromatografia Líquida , Neurotransmissores/análise , Espectrometria de Massas em Tandem , Animais , Técnicas de Química Analítica/instrumentação , Interações Hidrofóbicas e Hidrofílicas , Lymnaea/química , Reprodutibilidade dos TestesRESUMO
The epidermal mucus covering the surface of a snail represents an important barrier to trematode larvae attempting to penetrate the snail and may play a role in mediating snail-trematode compatibility. In this study, Facioloides magna miracidia were exposed to mucus harvested from a compatible snail host, Lymnaea elodes (palustris), and from an incompatible snail, Helisoma trivolvis . In vitro treatment of freshly hatched miracidia with snail-derived mucus exerted dramatically different effects on larvae depending on snail species. At the lowest dilution of mucus tested (1:3) mean damage rates (tegumental damage and/or larval lysis and death) were as high as 100% for miracidia exposed to H. trivolvis mucus, while none of F. magna miracidia were damaged in L. elodes mucus. A dilution series for each snail species, and treatments with heat and proteinase K were performed to characterize the component(s) of mucus inducing the observed morphological changes. The damaging effects of H. trivolvis mucus were concentration dependent and completely abrogated by heat (65 C, 30 min) and proteinase treatment, strongly implicating a heat-labile protein(s) in mucus as the active cytotoxic agent(s). In contrast to our prediction that miracidial contact with mucus of compatible L. elodes would trigger larval transformation, mucus from either snail species tested exhibited little to no activity. Overall these data demonstrate the presence of a potent cytotoxic protein-like factor in the mucus of F. magna -incompatible H. trivolvis , and its absence in the mucus of the compatible snail, L. elodes . This finding supports the notion that the epidermal mucus layer may be serving as an important determinant of larval trematode-snail compatibility.
Assuntos
Fasciolidae/fisiologia , Muco/fisiologia , Caramujos/química , Análise de Variância , Animais , Cervos/parasitologia , Fígado/parasitologia , Lymnaea/química , Lymnaea/parasitologia , Muco/metabolismo , Óvulo , Lagoas , Caramujos/parasitologiaRESUMO
The freshwater pulmonate snail, Lymnaea stagnalis, is the most sensitive aquatic organism tested to date for Ni. We undertook a series of experiments to investigate the underlying mechanism(s) for this observed hypersensitivity. Consistent with previous experiments, juvenile snail growth in a 21-day exposure was reduced by 48% relative to the control when exposed to 1.3 µg l(-1) Ni (EC20 less than the lowest concentration tested). Ca(2+) homeostasis was significantly disrupted by Ni exposure as demonstrated by reductions in net Ca(2+) uptake, and reductions in Ca(2+) concentrations in the hemolymph and soft tissues. We also observed reduced soft tissue [Mg(2+)]. Snails underwent a significant alkalosis with hemolymph pH increasing from 8.1 to 8.3 and hemolymph TCO2 increasing from 19 to 22 mM in control versus Ni-exposed snails, respectively. Unlike in previous studies with Co and Pb, snail feeding rates were found to be unaffected by Ni at the end of the exposure. Snails accumulated Ni in the soft tissue in a concentration-dependent manner, and Ni uptake experiments with (63)Ni revealed a biphasic uptake profile - a saturable high affinity component at low exposure concentrations (36-189 nM) and a linear component at the high exposure concentrations (189-1,897 nM). The high affinity transport system had an apparent Km of 89 nM Ni(2+) and Vmax of 2.4 nmol g(-1)h(-1). This equates to a logK of 7.1, significantly higher than logK's (2.6-5.2) for any other aquatic organisms evaluated to date, which will have implications for Biotic Ligand Model development. Finally, pharmacological inhibitors that block Ca(2+) uptake pathways in snails did not inhibit Ni uptake, suggesting that the uptake of Ni does not occur via Ca(2+) uptake pathways. As with Cu and Pb, the exact mechanism for the significant disruption in Ca(2+) homeostasis and reduction in juvenile snail growth remains unknown.
Assuntos
Homeostase/efeitos dos fármacos , Lymnaea/efeitos dos fármacos , Níquel/metabolismo , Níquel/toxicidade , Poluentes Químicos da Água/metabolismo , Poluentes Químicos da Água/toxicidade , Equilíbrio Ácido-Base/efeitos dos fármacos , Animais , Cálcio/análise , Água Doce , Íons/metabolismo , Lymnaea/química , Lymnaea/crescimento & desenvolvimento , Magnésio/análiseRESUMO
Neuronal nicotinic receptors (nAChRs) belong to the Cys-loop family of ligand-gated ion channels and are formed from five subunits either as homologous or heterologous, oligomeric receptors, and are of interest as targets for treatment of a variety of central and peripheral nervous system disorders. Using a model of the homopentameric α7 nAChR extracellular region derived from the homologous acetylcholine binding protein (AChBP) from Aplysia California, binding modes of structurally diverse, high affinity α7 ligands were examined by docking to the orthosteric ligand binding domain. While all α7 ligands show similar interactions between the essential positively charged cationic center of the ligand and αTRP147 of the receptor (i.e., hydrogen bond to the tryptophan backbone carbonyl and cation-π interaction), docked poses of various ligands show the potential to interact with three additional regions within the binding domain, identified as regions 1, 2, and 3. Region 1 is located in the vicinity of Loop-E, involves ligand-protein interactions via a network of water-mediated hydrogen bonds, and is analogous to the region where pyridinyl groups are located in many of the AChBP-nicotinic ligand cocrystal structures. Ligands interacting with region 2 probe an area that spans from Loop-E to Loops-D and -F and may contribute to α7-selectivity over other nAChR subtypes. Several high affinity α7 ligands show strong interactions in this region. Region 3 is located near Loop-F of the protein and is analogous to an area involved in binding of an active metabolite derived from DMXBA, in an AChBP cocrystal structure. It appears that π-π interactions contribute to binding affinities of α7 nAChR ligands in this latter region, and further, this region may also contribute to α7-selectivity over other nAChR subtypes. Analysis of the resulting poses suggests that compounds with high α7 binding affinity do not require interactions across all regions simultaneously, but that interactions in multiple regions may enhance ligand binding and increase selectivity. Our results provide insight for further development of selective α7 nAChR ligands and may prove useful for the design of novel scaffolds for specific nicotinic therapeutic agents.
Assuntos
Acetilcolina/química , Proteínas de Transporte/química , Colinérgicos/química , Subunidades Proteicas/química , Receptores Nicotínicos/química , Animais , Aplysia/química , Sítios de Ligação , Cristalografia por Raios X , Desenho de Fármacos , Ligação de Hidrogênio , Ligantes , Lymnaea/química , Simulação de Acoplamento Molecular , Neurônios/metabolismo , Ligação Proteica , Multimerização Proteica , Estrutura Secundária de Proteína , Homologia Estrutural de Proteína , Trítio , Triptofano/química , Receptor Nicotínico de Acetilcolina alfa7RESUMO
The nicotinic acetylcholine receptors (nAChRs) are a family of closely related but pharmacologically distinct neurotransmitter-gated ion channels. They are therapeutic targets for a wide range of neurological disorders, and a key issue in drug development is selective targeting among the more than 20 subtypes of nAChRs that are known. The present work evaluates a proposed hydrogen bonding interaction involving a residue known as the "loop B glycine" that distinguishes receptors that are highly responsive to ACh and nicotine from those that are much less so. We have performed structure-function studies on the loop B site, including unnatural amino acid mutagenesis, in three different nAChR subtypes and found that the correlation between agonist potency and this residue is strong. Low potency receptor subtypes have a glycine at this key site, and mutation to a residue with a side chain converts a low potency receptor to a high potency receptor. Innately high potency receptors have a lysine at the loop B site and show a decrease in potency for the reverse mutation (i.e., introducing a glycine). This residue lies outside of the agonist binding site, and studies of other residues at the agonist binding site show that the details of how changes at the loop B glycine site impact agonist potency vary for differing receptor subtypes. This suggests a model in which the loop B residue influences the global shape of the agonist binding site rather than modulating any specific interaction.
Assuntos
Lymnaea/química , Agonistas Nicotínicos/química , Agonistas Nicotínicos/farmacologia , Receptores Nicotínicos/química , Receptores Nicotínicos/metabolismo , Sequência de Aminoácidos , Animais , Sítios de Ligação , Glicina/química , Ligação de Hidrogênio , Lymnaea/metabolismo , Modelos Moleculares , Dados de Sequência Molecular , Alinhamento de SequênciaRESUMO
Long-term memory formation requires "de novo" expression and post-translational modification of many proteins. Understanding the temporal and spatial regulatory pattern of these proteins is fundamental to decoding the molecular basis of learning and memory. We characterized changes in expression, phosphorylation, and glycosylation of CNS proteins after operant conditioning in pond snail Lymnaea stagnalis. The phosphorylation and the glycosylation levels of proteins, measured by the ratio of Pro-Q Diamond (phosphoproteins) or Pro-Q Emerald (glycoproteins) vs. SYPRO-Ruby (total proteins) signals, increased during memory formation. Proteins whose modulation of phosphorylation might be involved in learning and memory were identified by mass spectrometry (MS) and are associated with cytoskeleton, glutamine cycle, energy metabolism, G-protein signaling, neurotransmitter release regulation, iron transport, protein synthesis, and cell division. Phosphorylation of actin increased during memory formation. To identify proteins whose expression levels changed in long-term memory formation we used two-dimensional difference gel electrophoresis followed by MS. The up-regulated proteins are mostly associated with lipoprotein and cholesterol metabolism, protein synthesis and degradation, cytoskeleton, nucleic acid synthesis, and energy supply. The down-regulated proteins are enzymes of aspartic acid metabolism involved in regulation of protein synthesis. Our proteomic analyses have revealed a number of candidate proteins associated with memory formation. These findings provide new directions for further investigation into the signaling networks required for memory formation and consolidation.
Assuntos
Aprendizagem da Esquiva/fisiologia , Sistema Nervoso Central/metabolismo , Condicionamento Operante/fisiologia , Lymnaea/metabolismo , Memória/fisiologia , Proteínas do Tecido Nervoso/biossíntese , Animais , Sistema Nervoso Central/química , Sistema Nervoso Central/fisiologia , Glicosilação , Lymnaea/química , Lymnaea/fisiologia , Proteínas do Tecido Nervoso/metabolismo , Fosfoproteínas/metabolismo , Fosfoproteínas/fisiologia , Fosforilação/fisiologiaRESUMO
The ecotoxicological impact of nitrate-induced photodegradation products of diuron and chlorotoluron was studied through monospecific biotests conducted in conjunction with experiments in outdoor aquatic mesocosms. Organisms representing three trophic levels were used: two heterotrophic microorganisms, the luminescent bacterium Vibrio fischeri and the ciliated protozoa Tetrahymena pyriformis, and one metazoa, the gastropod Lymnaea stagnalis. Among the variety of the phenylurea photoproducts, the N-formylated ones appeared clearly more toxic than the parent compounds towards the microorganisms, whereas the nitroderivatives showed a similar toxicity. Using photodegraded solutions of diuron, toxicity was maintained or even increased during disappearance of the initial herbicide, demonstrating that some of the photoproducts may have an impact additively or in synergy. Enzymatic biomarker assays performed on Lymnaea stagnalis exposed under monospecific conditions showed significant effects, due to the combination of nitrate with the pesticide and its photoproducts. A positive impact on snail fecundity was observed with chlorotoluron both under monospecific laboratory and integrated mesocosm conditions. Oviposition stimulation took place when first- and second-generation photoproducts were predominant.
Assuntos
Diurona/química , Diurona/toxicidade , Nitratos/química , Compostos de Fenilureia/química , Compostos de Fenilureia/toxicidade , Aliivibrio fischeri/química , Aliivibrio fischeri/efeitos dos fármacos , Animais , Ecotoxicologia , Herbicidas/química , Herbicidas/toxicidade , Lymnaea/química , Lymnaea/efeitos dos fármacos , Fotólise , Compostos de Amônio Quaternário/química , Tetrahymena pyriformis/química , Tetrahymena pyriformis/efeitos dos fármacos , Água/químicaRESUMO
Seminal fluid is an important part of the ejaculate of internally fertilizing animals. This fluid contains substances that nourish and activate sperm for successful fertilization. Additionally, it contains components that influence female physiology to further enhance fertilization success of the sperm donor, possibly beyond the recipient's optimum. Although evidence for such substances abounds, few studies have unraveled their identities, and focus has been exclusively on separate-sex species. We present the first detailed study into the seminal fluid composition of a hermaphrodite (Lymnaea stagnalis). Eight novel peptides and proteins were identified from the seminal-fluid-producing prostate gland and tested for effects on oviposition, hatching and consumption. The gene for the protein found to suppress egg mass production, Ovipostatin, was sequenced, thereby providing the first fully-characterized seminal fluid substance in a simultaneous hermaphrodite. Thus, seminal fluid peptides and proteins have evolved and can play a crucial role in sexual selection even when the sexes are combined.
Assuntos
Transtornos do Desenvolvimento Sexual , Lymnaea/fisiologia , Oviposição , Sêmen/química , Proteínas de Plasma Seminal/fisiologia , Animais , Feminino , Genes , Humanos , Lymnaea/química , Masculino , Próstata , Proteínas de Plasma Seminal/análise , Proteínas de Plasma Seminal/genética , Análise de Sequência de DNARESUMO
Most studies have reported negative carbon and nitrogen isotope fractionations between hosts and parasites, but isotope values have not yet been determined for many parasite species, such as trematodes, due to their relatively small body sizes. We investigated the carbon and nitrogen isotope values of freshwater snails and trematode parasites by using a method for organisms with very small body sizes. We found negative isotope fractionation values between host snails and trematode parasites, similar to published values for other parasite groups with larger body sizes, which suggest that the mechanisms for determining isotope fractionations between hosts and parasites provide similar results.
Assuntos
Isótopos de Carbono/análise , Lymnaea/química , Lymnaea/parasitologia , Isótopos de Nitrogênio/análise , Trematódeos/química , Análise de Variância , Animais , Echinostomatidae/química , Água Doce , Interações Hospedeiro-ParasitaRESUMO
This study reports the comparative molecular modeling, docking and dynamic simulations of human alpha9alpha10 nicotinic acetylcholine receptors complexed with acetylcholine, nicotine and alpha-conotoxin RgIA, using as templates the crystal structures of Aplysia californica and Lymnaea stagnalis acetylcholine binding proteins. The molecular dynamics simulations showed that Arg112 in the complementary alpha10(-) subunit, is a determinant for recognition in the site that binds small ligands. However, Glu195 in the principal alpha9(+), and Asp114 in the complementary alpha10(-) subunit, might confer the potency and selectivity to alpha-conotoxin RgIA when interacting with Arg7 and Arg9 of this ligand.
Assuntos
Modelos Moleculares , Receptores Nicotínicos/química , Acetilcolina/química , Aminoácidos , Animais , Aplysia/química , Sítios de Ligação , Simulação por Computador , Conotoxinas/química , Humanos , Lymnaea/química , Nicotina/química , Ligação ProteicaRESUMO
To estimate isotopic changes caused by trematode parasites within a host, we investigated changes in the carbon and nitrogen isotope ratios of the freshwater snail Lymnaea stagnalis infected by trematode larvae. We measured carbon and nitrogen stable isotopes within the foot, gonad, and hepatopancreas of both infected and uninfected snails. There was no significant difference in the delta13C and delta15N values of foot and gonad between infected and uninfected snails; thus, trematode parasite infections may not cause changes in snail diets. However, in the hepatopancreas, delta15N values were significantly higher in infected than in uninfected snails. The 15N enrichment in the hepatopancreas of infected snails is caused by the higher 15N ratio in parasite tissues. Using an isotope-mixing model, we roughly estimated that the parasites in the hepatopancreas represented from 0.8 to 3.4% of the total snail biomass, including the shell.
Assuntos
Lymnaea/parasitologia , Trematódeos/fisiologia , Animais , Isótopos de Carbono/análise , Dieta , Ecossistema , Comportamento Alimentar , Hepatopâncreas/parasitologia , Interações Hospedeiro-Parasita , Lymnaea/química , Isótopos de Nitrogênio/análise , Oocistos/fisiologia , Rios , SibériaRESUMO
Neonicotinoid insecticides, which act on nicotinic acetylcholine receptors (nAChRs) in a variety of ways, have extremely low mammalian toxicity, yet the molecular basis of such actions is poorly understood. To elucidate the molecular basis for nAChR-neonicotinoid interactions, a surrogate protein, acetylcholine binding protein from Lymnaea stagnalis (Ls-AChBP) was crystallized in complex with neonicotinoid insecticides imidacloprid (IMI) or clothianidin (CTD). The crystal structures suggested that the guanidine moiety of IMI and CTD stacks with Tyr185, while the nitro group of IMI but not of CTD makes a hydrogen bond with Gln55. IMI showed higher binding affinity for Ls-AChBP than that of CTD, consistent with weaker CH-pi interactions in the Ls-AChBP-CTD complex than in the Ls-AChBP-IMI complex and the lack of the nitro group-Gln55 hydrogen bond in CTD. Yet, the NH at position 1 of CTD makes a hydrogen bond with the backbone carbonyl of Trp143, offering an explanation for the diverse actions of neonicotinoids on nAChRs.
Assuntos
Guanidinas/metabolismo , Imidazóis/metabolismo , Modelos Moleculares , Nitrocompostos/metabolismo , Receptores Nicotínicos/química , Receptores Nicotínicos/metabolismo , Tiazóis/metabolismo , Animais , Sítios de Ligação , Lymnaea/química , Lymnaea/metabolismo , Conformação Molecular , Dados de Sequência Molecular , Neonicotinoides , Relação Estrutura-AtividadeRESUMO
Flame atomic absorption spectrometry was performed to determine the alteration in the concentrations of metallic ion Pb, Zn, K, Na, Fe, Cu and Co in the soft parts of the Lymnaea natalensis snails shedding Fasciola gigantica cercariae and to determine the alteration in the concentration of Ca in the soft parts and shells of the same snails. The Co was found to be present at concentration level below the detection limits of the analytical method used. Regarding detected elements, three elements Zn, K and Cu were found to be present at significantly higher concentrations in cercariae-shedding snails compared with uninfected snails. Two elements, Pb and Na, showed significant decrease in cercariae-shedding snails compared to uninfected ones. The concentration of Fe showed non-significant increase. The results showed significant lowering in the calcium content of the shells and soft parts of cercariae-shedding snails relative to the calcium content in the uninfected ones. The obtained results and the hypothesis of hypercalcification in shells of infected snails were discussed.
Assuntos
Fasciola/fisiologia , Lymnaea/química , Lymnaea/parasitologia , Animais , Calcificação Fisiológica , Cálcio/análise , Bovinos , Cobre/análise , Ferro/análise , Chumbo/análise , Potássio/análise , Sódio/análise , Espectrofotometria Atômica , Zinco/análiseRESUMO
We investigate the interactions between the long chain alpha-cobratoxin (Cbtx) and the nicotinic acetylcholine receptor using a rigid body docking procedure. The method, (i) reproduces the binding of Cbtx to Lymnea acetylcholine-binding protein (AChBP); (ii) shows that most of the structures of AChBP obtained in the presence of antagonists are compatible with Cbtx binding; and (iii) reveals a complex between Cbtx and muscle nAChR that corresponds to the basal "resting" state conformation. The structures are made available for further understanding of the allosteric transitions of the nAChR as well as for drug design.
Assuntos
Proteínas Neurotóxicas de Elapídeos/química , Proteínas Neurotóxicas de Elapídeos/metabolismo , Elapidae/metabolismo , Receptores Nicotínicos/química , Receptores Nicotínicos/metabolismo , Animais , Proteínas de Transporte/antagonistas & inibidores , Proteínas de Transporte/química , Proteínas de Transporte/metabolismo , Cristalografia por Raios X , Lymnaea/química , Lymnaea/metabolismo , Modelos Moleculares , Músculos/metabolismo , Antagonistas Nicotínicos/farmacologia , Ligação Proteica , Estrutura Quaternária de Proteína , Estrutura Terciária de ProteínaRESUMO
Even tough the central nervous system (CNS) of gastropods has long been used as a model for studying different neuronal networks underlying behaviors, there is only little information on the molecular components of the extracellular matrix (ECM) of the nervous tissue. Therefore, the aim of the present study was to identify some of the ECM molecules by acid-base histochemistry. Staining with alcian blue at strong acidic pH, and with acridine orange at different pH and salt concentrations was carried out on cryostat sections taken from CNS preparations of adult specimens of the terrestrial snail, Helix pomatia, and the aquatic species, Lymnaea stagnolis, in order to visualize mild (carboxyl) and strong (sulphate) acidic groups, which are characteristic for different glucosaminoglycans. According to our findings, sulphated proteoglycans were abundant in the periganglionic sheath of both species, and they also occurred in the neuropil of Helix, whereas they were absent in Lymnaea. The interperikaryonal space contained mainly carboxyl residues, which might refer to the presence of hyaluronic acid. It is concluded that the ECM of the snail CNS, similarly to that in vertebrates, is partly composed of polymer macromolecules of different chemical properties. It is suggested that adaptation to environmental conditions and/or altered neuronal plasticity are responsible for the differences found in chemical characters of the ECM molecules between the two snail species.