RESUMO
Basement membranes (BMs) are specialized sheets of extracellular matrix that underlie epithelial and endothelial tissues. BMs regulate the traffic of cells and molecules between compartments, and participate in signaling, cell migration, and organogenesis. The dynamics of mammalian BMs, however, are poorly understood, largely due to a lack of models in which core BM components are endogenously labeled. Here, we describe the mTurquoise2-Col4a1 mouse in which we fluorescently tag collagen IV, the main component of BMs. Using an innovative planar-sagittal live imaging technique to visualize the BM of developing skin, we directly observe BM deformation during hair follicle budding and basal progenitor cell divisions. The BM's inherent pliability enables dividing cells to remain attached to and deform the BM, rather than lose adhesion as generally thought. Using FRAP, we show BM collagen IV is extremely stable, even during periods of rapid epidermal growth. These findings demonstrate the utility of the mTurq2-Col4a1 mouse to shed new light on mammalian BM developmental dynamics.
Assuntos
Membrana Basal , Colágeno Tipo IV , Matriz Extracelular , Animais , Camundongos , Membrana Basal/crescimento & desenvolvimento , Colágeno Tipo IV/genética , Colágeno Tipo IV/metabolismo , Matriz Extracelular/metabolismo , Corantes Fluorescentes , Folículo Piloso/crescimento & desenvolvimento , Células-TroncoRESUMO
RESEARCH QUESTION: Are there differences in the composition and structure of the basal lamina surrounding follicles in prepubertal versus adult human ovarian tissue? DESIGN: Frozen-thawed human ovarian tissue from six prepubertal and seven adult patients was divided into three fragments in each case: two for non-grafted tissue evaluation and one for long-term xenografting to mice. Collagen IV and laminin expression were investigated by immunohistochemistry before and after grafting. The basal lamina was analysed by transmission electron microscopy on frozen-thawed tissue. RESULTS: In frozen-thawed tissue, collagen IV was significantly less expressed around prepubertal follicles than around adult follicles (primordial, Pâ¯=â¯0.02; intermediate/growing follicles, Pâ¯=â¯0.03), while laminin was significantly more expressed (primordial, Pâ¯=â¯0.03; intermediate, Pâ¯=â¯0.01). Collagen IV expression was significantly higher around prepubertal primordial follicles in grafted tissue than in non-grafted tissue, reaching similar levels to those in adult tissue. Ultrastructure analysis showed the basal lamina around follicles in prepubertal frozen-thawed tissue to be rather patchy and thinner than around adult follicles (primordial/intermediate, Pâ¯=â¯0.001; primary, Pâ¯=â¯0.02). CONCLUSIONS: In frozen-thawed tissue, the basal lamina around prepubertal follicles is less mature than around adult follicles, but it becomes similar in both prepubertal and adult subjects after grafting. Grafting could therefore induce maturation of the basal lamina around prepubertal follicles.
Assuntos
Membrana Basal/ultraestrutura , Criopreservação , Ovário/ultraestrutura , Desenvolvimento Sexual , Transplante Heterólogo , Adulto , Animais , Membrana Basal/crescimento & desenvolvimento , Membrana Basal/metabolismo , Criança , Pré-Escolar , Colágeno Tipo IV/metabolismo , Feminino , Humanos , Lactente , Laminina/metabolismo , Camundongos SCID , Ovário/crescimento & desenvolvimento , Ovário/metabolismo , Adulto JovemRESUMO
RASA1, a negative regulator of Ras-MAPK signaling, is essential for the development and maintenance of lymphatic vessel valves. However, whether RASA1 is required for the development and maintenance of lymphovenous valves (LVV) and venous valves (VV) is unknown. In this study, we show that induced disruption of Rasa1 in mouse embryos did not affect initial specification of LVV or central VV, but did affect their continued development. Similarly, a switch to expression of a catalytically inactive form of RASA1 resulted in impaired LVV and VV development. Blocked development of LVV was associated with accumulation of the basement membrane protein, collagen IV, in LVV-forming endothelial cells (EC), and could be partially or completely rescued by MAPK inhibitors and drugs that promote collagen IV folding. Disruption of Rasa1 in adult mice resulted in venous hypertension and impaired VV function that was associated with loss of EC from VV leaflets. In conclusion, RASA1 functions as a negative regulator of Ras signaling in EC that is necessary for EC export of collagen IV, thus permitting the development of LVV and the development and maintenance of VV.
Assuntos
Desenvolvimento Embrionário/genética , Organogênese/genética , Válvulas Venosas/crescimento & desenvolvimento , Proteína p120 Ativadora de GTPase/genética , Animais , Membrana Basal/crescimento & desenvolvimento , Membrana Basal/metabolismo , Colágeno Tipo IV/genética , Embrião de Mamíferos , Células Endoteliais/citologia , Vasos Linfáticos/metabolismo , Camundongos , Válvulas Venosas/metabolismoRESUMO
Laminin-α2 related congenital muscular dystrophy (LAMA2-CMD) is a fatal muscle disease caused by mutations in the LAMA2 gene. Laminin-α2 is critical for the formation of laminin-211 and -221 heterotrimers in the muscle basal lamina. LAMA2-CMD patients exhibit hypotonia from birth and progressive muscle loss that results in developmental delay, confinement to a wheelchair, respiratory insufficiency and premature death. There is currently no cure or effective treatment for LAMA2-CMD. Several studies have shown laminin-111 can serve as an effective protein-replacement therapy for LAMA2-CMD. Studies have demonstrated early treatment with laminin-111 protein results in an increase in life expectancy and improvements in muscle pathology and function. Since LAMA2-CMD patients are often diagnosed after advanced disease, it is unclear if laminin-111 protein therapy at an advanced stage of the disease can have beneficial outcomes. In this study, we tested the efficacy of laminin-111 protein therapy after disease onset in a mouse model of LAMA2-CMD. Our results showed laminin-111 treatment after muscle disease onset increased life expectancy, promoted muscle growth and increased muscle stiffness. Together these studies indicate laminin-111 protein therapy either early or late in the disease process could serve as an effective protein replacement therapy for LAMA2-CMD.
Assuntos
Laminina/farmacologia , Doenças Musculares/genética , Distrofias Musculares/genética , Animais , Membrana Basal/efeitos dos fármacos , Membrana Basal/crescimento & desenvolvimento , Modelos Animais de Doenças , Humanos , Laminina/genética , Camundongos , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/crescimento & desenvolvimento , Doenças Musculares/patologia , Distrofias Musculares/patologia , Mutação/genéticaRESUMO
Laminin-γ1 is required for early embryonic development; however, the need for laminin-γ1 synthesis in adulthood is unknown. A global and inducible mouse model of laminin-γ1 deficiency was generated to address this question. Genetic ablation of the Lamc1 gene in adult mice was rapidly lethal. Despite global Lamc1 gene deletion in tamoxifen-induced mutant mice, there was minimal change in total cardiac, pulmonary, hepatic or renal laminin protein. In contrast, laminin-γ1 was significantly depleted in the small intestines, which showed crypt hyperplasia and dissociation of villous epithelium from adjacent mesenchyme. We conclude that the physiologic requirement for laminin-γ1 synthesis in adult mice is dependent on a tissue-specific basal rate of laminin-γ1 turnover that results in rapid depletion of laminin-γ1 in the intestine.
Assuntos
Desenvolvimento Embrionário/genética , Intestinos/crescimento & desenvolvimento , Laminina/genética , Animais , Membrana Basal/crescimento & desenvolvimento , Membrana Basal/metabolismo , Feminino , Laminina/biossíntese , Fígado/metabolismo , CamundongosRESUMO
Organs are sculpted by extracellular as well as cell-intrinsic forces, but how collective cell dynamics are orchestrated in response to environmental cues is poorly understood. Here we apply advanced image analysis to reveal extracellular matrix-responsive cell behaviors that drive elongation of the Drosophila follicle, a model system in which basement membrane stiffness instructs three-dimensional tissue morphogenesis. Through in toto morphometric analyses of wild type and round egg mutants, we find that neither changes in average cell shape nor oriented cell division are required for appropriate organ shape. Instead, a major element is the reorientation of elongated cells at the follicle anterior. Polarized reorientation is regulated by mechanical cues from the basement membrane, which are transduced by the Src tyrosine kinase to alter junctional E-cadherin trafficking. This mechanosensitive cellular behavior represents a conserved mechanism that can elongate edgeless tubular epithelia in a process distinct from those that elongate bounded, planar epithelia.
Assuntos
Drosophila/crescimento & desenvolvimento , Matriz Extracelular/química , Folículo Ovariano/crescimento & desenvolvimento , Animais , Membrana Basal/química , Membrana Basal/crescimento & desenvolvimento , Membrana Basal/metabolismo , Caderinas/genética , Caderinas/metabolismo , Polaridade Celular , Forma Celular , Drosophila/química , Drosophila/genética , Drosophila/metabolismo , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Matriz Extracelular/genética , Matriz Extracelular/metabolismo , Feminino , Folículo Ovariano/metabolismoRESUMO
Tissue mechanics play a crucial role in organ development. They rely on the properties of cells and the extracellular matrix (ECM), but the relative physical contribution of cells and ECM to morphogenesis is poorly understood. Here, we have analyzed the behavior of the peripodial epithelium (PE) of the Drosophila leg disc in the light of the dynamics of its cellular and ECM components. The PE undergoes successive changes during leg development, including elongation, opening and removal to free the leg. During elongation, we found that the ECM and cell layer are progressively uncoupled. Concomitantly, the tension, mainly borne by the ECM at first, builds up in the cell monolayer. Then, each layer of the peripodial epithelium is removed by an independent mechanism: while the ECM layer withdraws following local proteolysis, cellular monolayer withdrawal is independent of ECM degradation and is driven by myosin II-dependent contraction. These results reveal a surprising physical and functional cell-matrix uncoupling in a monolayer epithelium under tension during development.This article has an associated 'The people behind the papers' interview.
Assuntos
Drosophila melanogaster/embriologia , Epitélio/embriologia , Epitélio/crescimento & desenvolvimento , Matriz Extracelular/fisiologia , Membro Posterior/embriologia , Morfogênese/fisiologia , Animais , Animais Geneticamente Modificados , Membrana Basal/embriologia , Membrana Basal/crescimento & desenvolvimento , Fenômenos Biomecânicos , Padronização Corporal/fisiologia , Comunicação Celular/fisiologia , Proliferação de Células , Drosophila melanogaster/crescimento & desenvolvimento , Embrião não Mamífero , Membro Posterior/crescimento & desenvolvimento , Miosina Tipo II/fisiologia , Proteólise , Tensão SuperficialRESUMO
Basement membrane plays a foundational role in the structure and maintenance of many tissues throughout the animal kingdom. In addition to signaling to cells through cell-surface receptors, basement membrane directly influences the development and maintenance of organ shape via its mechanical properties. The mechanical properties of basement membrane are dictated by its composition, geometry, and crosslinking. Distinguishing between the ways the basement membrane influences morphology in vivo poses a major challenge. Drosophila melanogaster, already established as a powerful model for the analysis of cell signaling, has in recent years emerged as a tractable model for understanding the roles of basement membrane stiffness in vivo, in shaping and maintaining the morphology of tissues and organs. In addition to the plethora of genetic tools available in flies, the major proteins found in vertebrate basement membranes are all present in Drosophila. Furthermore, Drosophila has fewer copies of the genes encoding these proteins, making flies more amenable to genetic manipulation than vertebrate models. Because the development of Drosophila organs has been well-characterized, these different organ systems offer a variety of contexts for analyzing the role of basement membrane in development. The developing egg chamber and central nervous system, for example, have been important models for assessing the role of basement membrane stiffness in influencing organ shape. Studies in the nervous system have also shown how basement membrane stiffness can influence cellular migration in vivo. Finally, work in the imaginal wing disc has illuminated a distinct mechanism by which basement membrane can alter organ shape and size, by sequestering signaling ligands. This mini-review highlights the recent discoveries pertaining to basement membrane mechanics during Drosophila development.
Assuntos
Membrana Basal/crescimento & desenvolvimento , Drosophila melanogaster/genética , Organogênese/genética , Receptores de Superfície Celular/genética , Animais , Membrana Basal/metabolismo , Movimento Celular/genética , Drosophila melanogaster/crescimento & desenvolvimento , Sistema Nervoso/crescimento & desenvolvimento , Sistema Nervoso/metabolismo , Óvulo/crescimento & desenvolvimento , Óvulo/metabolismo , Transdução de Sinais/genéticaRESUMO
Basement membranes (BMs) are thin dense sheets of extracellular matrix that surround most tissues. When the BMs of neighboring tissues come into contact, they usually slide along one another and act to separate tissues and organs into distinct compartments. However, in certain specialized regions, the BMs of neighboring tissues link, helping to bring tissues together. These BM connections can be transient, such as during tissue fusion events in development, or long-term, as with adult tissues involved with filtration, including the blood brain barrier and kidney glomerulus. The transitory nature of these connections in development and the complexity of tissue filtration systems in adults have hindered the understanding of how juxtaposed BMs fasten together. The recent identification of a BM-BM adhesion system in C. elegans, termed B-LINK (BM linkage), however, is revealing cellular and extracellular matrix components of a nascent tissue adhesion system. We discuss insights gained from studying the B-LINK tissue adhesion system in C. elegans, compare this adhesion with other BM-BM connections in Drosophila and vertebrates, and outline important future directions towards elucidating this fascinating and poorly understood mode of adhesion that joins neighboring tissues.
Assuntos
Membrana Basal/metabolismo , Matriz Extracelular/genética , Aderências Teciduais/genética , Animais , Membrana Basal/crescimento & desenvolvimento , Barreira Hematoencefálica/crescimento & desenvolvimento , Barreira Hematoencefálica/metabolismo , Caenorhabditis elegans/genética , Comunicação Celular/genética , Compartimento Celular/genética , Drosophila/genética , Matriz Extracelular/metabolismo , Humanos , Glomérulos Renais/crescimento & desenvolvimento , Glomérulos Renais/metabolismoRESUMO
How some animals regenerate missing body parts is not well understood. Taking advantage of the zebrafish caudal fin model, we performed a global unbiased time-course transcriptomic analysis of fin regeneration. Biostatistics analyses identified extracellular matrix (ECM) as the most enriched gene sets. Basement membranes (BMs) are specialized ECM structures that provide tissues with structural cohesion and serve as a major extracellular signaling platform. While the embryonic formation of BM has been extensively investigated, its regeneration in adults remains poorly studied. We therefore focused on BM gene expression kinetics and showed that it recapitulates many aspects of development. As such, the re-expression of the embryonic col14a1a gene indicated that col14a1a is part of the regeneration-specific program. We showed that laminins and col14a1a genes display similar kinetics and that the corresponding proteins are spatially and temporally controlled during regeneration. Analysis of our CRISPR/Cas9-mediated col14a1a knockout fish showed that collagen XIV-A contributes to timely deposition of laminins. As changes in ECM organization can affect tissue mechanical properties, we analyzed the biomechanics of col14a1a-/- regenerative BM using atomic force microscopy (AFM). Our data revealed a thinner BM accompanied by a substantial increase of the stiffness when compared to controls. Further AFM 3D-reconstructions showed that BM is organized as a checkerboard made of alternation of soft and rigid regions that is compromised in mutants leading to a more compact structure. We conclude that collagen XIV-A transiently acts as a molecular spacer responsible for BM structure and biomechanics possibly by helping laminins integration within regenerative BM.
Assuntos
Nadadeiras de Animais/crescimento & desenvolvimento , Membrana Basal/crescimento & desenvolvimento , Colágeno/genética , Regeneração/genética , Proteínas de Peixe-Zebra/genética , Nadadeiras de Animais/ultraestrutura , Animais , Membrana Basal/ultraestrutura , Sistemas CRISPR-Cas , Matriz Extracelular/genética , Matriz Extracelular/ultraestrutura , Proteínas da Matriz Extracelular/genética , Regulação da Expressão Gênica no Desenvolvimento/genética , Cinética , Transcriptoma/genética , Cicatrização/genética , Peixe-Zebra/genética , Peixe-Zebra/crescimento & desenvolvimentoRESUMO
The ability of skin to act as a barrier is primarily determined by cells that maintain the continuity and integrity of skin and restore it after injury. Cutaneous wound healing in adult mammals is a complex multi-step process that involves overlapping stages of blood clot formation, inflammation, re-epithelialization, granulation tissue formation, neovascularization, and remodeling. Under favorable conditions, epidermal regeneration begins within hours after injury and takes several days until the epithelial surface is intact due to reorganization of the basement membrane. Regeneration relies on numerous signaling cues and on multiple cellular processes that take place both within the epidermis and in other participating tissues. A variety of modulators are involved, including growth factors, cytokines, matrix metalloproteinases, cellular receptors, and extracellular matrix components. Here we focus on the involvement of the extracellular matrix proteins that impact epidermal regeneration during wound healing.
Assuntos
Matriz Extracelular/genética , Pele/crescimento & desenvolvimento , Cicatrização/genética , Ferimentos e Lesões/genética , Membrana Basal/crescimento & desenvolvimento , Membrana Basal/metabolismo , Movimento Celular/genética , Células Epidérmicas , Matriz Extracelular/patologia , Humanos , Queratinócitos/metabolismo , Queratinócitos/patologia , Reepitelização , Transdução de Sinais/genética , Pele/lesões , Ferimentos e Lesões/patologiaRESUMO
This contribution describes the growth of oocytes, addressing the formation of structures that compose the follicular complex, as well as the remodeling of the extracellular matrix, specifically laminin, fibronectin and type IV collagen during gonadal maturation. Thirty-seven females of the Acari zebra fish, Hypancistrus zebra were captured and the ovaries were submitted to histological processing for light and electron microscopy and immunohistochemistry techniques. Oogonia and four stages (I - IV) of oocytes were distinguished, and structures such as the postovulatory follicle and atretic oocytes (initial and advanced atresia) were observed. The follicular complex consists of the mature oocyte, zona radiata (Zr1, Zr2 and Zr3), follicular cells, basement membrane and theca. During oocyte growth, proteins of the extracellular matrix showed different intensities of staining. Based on these observations, a model of oocyte growth is proposed to define specific characteristics of the oocyte and the remodeling of the extracellular matrix in the ovary of H. zebra. This model of oocyte growth can be extended to other species of ornamental fishes. This study contributes data for induced fertilization and eventual conservation of this species.
Assuntos
Matriz Extracelular/genética , Peixes/crescimento & desenvolvimento , Oogênese/genética , Folículo Ovariano/crescimento & desenvolvimento , Animais , Membrana Basal/crescimento & desenvolvimento , Membrana Basal/metabolismo , Matriz Extracelular/metabolismo , Feminino , Peixes/genética , Atresia Folicular/genética , Oócitos/crescimento & desenvolvimento , Oogônios/crescimento & desenvolvimento , Folículo Ovariano/metabolismo , Ovário/crescimento & desenvolvimento , Ovário/metabolismo , Ovulação/genética , Ovulação/fisiologia , Diferenciação Sexual/genéticaRESUMO
Age-related macular degeneration (AMD) remains a major cause of blindness, with dysfunction and loss of retinal pigment epithelium (RPE) central to disease progression. We engineered an RPE patch comprising a fully differentiated, human embryonic stem cell (hESC)-derived RPE monolayer on a coated, synthetic basement membrane. We delivered the patch, using a purpose-designed microsurgical tool, into the subretinal space of one eye in each of two patients with severe exudative AMD. Primary endpoints were incidence and severity of adverse events and proportion of subjects with improved best-corrected visual acuity of 15 letters or more. We report successful delivery and survival of the RPE patch by biomicroscopy and optical coherence tomography, and a visual acuity gain of 29 and 21 letters in the two patients, respectively, over 12 months. Only local immunosuppression was used long-term. We also present the preclinical surgical, cell safety and tumorigenicity studies leading to trial approval. This work supports the feasibility and safety of hESC-RPE patch transplantation as a regenerative strategy for AMD.
Assuntos
Células-Tronco Embrionárias Humanas/transplante , Degeneração Macular/terapia , Epitélio Pigmentado da Retina/transplante , Acuidade Visual/fisiologia , Idoso , Animais , Membrana Basal/diagnóstico por imagem , Membrana Basal/crescimento & desenvolvimento , Diferenciação Celular/genética , Feminino , Humanos , Degeneração Macular/diagnóstico por imagem , Degeneração Macular/patologia , Masculino , Camundongos , Pessoa de Meia-Idade , Epitélio Pigmentado da Retina/diagnóstico por imagem , Epitélio Pigmentado da Retina/crescimento & desenvolvimento , Transplante de Células-Tronco/efeitos adversos , Suínos , Tomografia de Coerência ÓpticaRESUMO
Satellite cells function as precursor cells in mature skeletal muscle homeostasis and regeneration. In healthy tissue, these cells are maintained in a state of quiescence by a microenvironment formed by myofibers and basement membrane in which LAMININs (LMs) form a major component. In the present study, we evaluated the satellite cell microenvironment in vivo and found that these cells are encapsulated by LMα2-5. We sought to recapitulate this satellite cell niche in vitro by culturing satellite cells in the presence of recombinant LM-E8 fragments. We show that treatment with LM-E8 promotes proliferation of satellite cells in an undifferentiated state, through reduced phosphorylation of JNK and p38. On transplantation into injured muscle tissue, satellite cells cultured with LM-E8 promoted the regeneration of skeletal muscle. These findings represent an efficient method of culturing satellite cells for use in transplantation through the recapitulation of the satellite cell niche using recombinant LM-E8 fragments.
Assuntos
Laminina/genética , Músculo Esquelético/crescimento & desenvolvimento , Regeneração/genética , Células Satélites de Músculo Esquelético/citologia , Animais , Membrana Basal/citologia , Membrana Basal/crescimento & desenvolvimento , Diferenciação Celular/genética , Linhagem Celular , Proliferação de Células/genética , Homeostase/genética , Humanos , Camundongos , Músculo Esquelético/citologia , Músculo Esquelético/transplante , Miofibrilas/genética , Células Satélites de Músculo Esquelético/transplante , Nicho de Células-Tronco/genéticaRESUMO
Cell invasion is a specialized cell behavior that likely co-evolved with the emergence of basement membranes in metazoans as a mechanism to break down the barriers that separate tissues. A variety of conserved and lineage-specific biological processes that occur during development and homeostasis rely on cell invasive behavior. Recent innovations in genome editing and live-cell imaging have shed some light on the programs that mediate acquisition of an invasive phenotype; however, comparative approaches among species are necessary to understand how this cell behavior evolved. Here, we discuss the contexts of cell invasion, highlighting both established and emerging model systems, and underscore gaps in our understanding of the evolution of this key cellular behavior.
Assuntos
Membrana Basal/citologia , Evolução Biológica , Caenorhabditis elegans/crescimento & desenvolvimento , Animais , Membrana Basal/crescimento & desenvolvimento , Caenorhabditis elegans/citologia , Comunicação Celular/genética , Edição de GenesRESUMO
Hypospadias, a congenital malformation of the penis characteristic of an abnormal urethral orifice, affects 1 in every 125 boys, and its incidence is rising. Herein we test the hypothesis that the basement membrane protein laminin α5 (LAMA5) plays a key role in the development of the mouse genital tubercle, the embryonic anlage of the external genitalia. Using standard histological analyses and electron microscopy, we characterized the morphology of the external genitalia in Lama5 knockout (LAMA5-KO) mouse embryos during both androgen-independent genital tubercle development and androgen-mediated sexual differentiation. We compared regulatory gene expression between control and LAMA5-KO by in situ hybridization. We also examined the epithelial structure of the mutant genital tubercle using immunofluorescence staining and histological analyses of semi-thin sections. We found that Lama5 was expressed in both ectodermal and endodermal epithelia of the cloaca. The LAMA5-KO displayed a profound external genital malformation in which the genital tubercle was underdeveloped with a large ectopic orifice at the proximal end. In older embryos, the urethra failed to form a tubular structure and was left completely exposed. These defects were not associated with a significant alteration in regulatory gene expression, but rather with a defective ectodermal epithelium and an abnormal disintegration of the cloacal membrane. We conclude that LAMA5 is required in the basement membrane to maintain normal architecture of the ventral ectoderm during genital tubercle development, which is essential for the formation of a tubular urethra. Perturbation of LAMA5, and possibly other basement membrane components, may cause hypospadias in humans.
Assuntos
Hipospadia/genética , Laminina/genética , Organogênese/genética , Diferenciação Sexual/genética , Androgênios/metabolismo , Animais , Membrana Basal/crescimento & desenvolvimento , Membrana Basal/metabolismo , Proliferação de Células/genética , Ectoderma/crescimento & desenvolvimento , Ectoderma/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Genitália/crescimento & desenvolvimento , Genitália/metabolismo , Humanos , Hipospadia/fisiopatologia , Masculino , Camundongos , Camundongos Knockout , Diferenciação Sexual/efeitos dos fármacos , Transdução de Sinais/genética , Uretra/crescimento & desenvolvimento , Uretra/metabolismoRESUMO
Invadopodia are specialized membrane protrusions composed of F-actin, actin regulators, signaling proteins, and a dynamically trafficked invadopodial membrane that drive cell invasion through basement membrane (BM) barriers in development and cancer. Due to the challenges of studying invasion in vivo, mechanisms controlling invadopodia formation in their native environments remain poorly understood. We performed a sensitized genome-wide RNAi screen and identified 13 potential regulators of invadopodia during anchor cell (AC) invasion into the vulval epithelium in C. elegans. Confirming the specificity of this screen, we identified the Rho GTPase cdc-42, which mediates invadopodia formation in many cancer cell lines. Using live-cell imaging, we show that CDC-42 localizes to the AC-BM interface and is activated by an unidentified vulval signal(s) that induces invasion. CDC-42 is required for the invasive membrane localization of WSP-1 (N-WASP), a CDC-42 effector that promotes polymerization of F-actin. Loss of CDC-42 or WSP-1 resulted in fewer invadopodia and delayed BM breaching. We also characterized a novel invadopodia regulator, gdi-1 (Rab GDP dissociation inhibitor), which mediates membrane trafficking. We show that GDI-1 functions in the AC to promote invadopodia formation. In the absence of GDI-1, the specialized invadopodial membrane was no longer trafficked normally to the invasive membrane, and instead was distributed to plasma membrane throughout the cell. Surprisingly, the pro-invasive signal(s) from the vulval cells also controls GDI-1 activity and invadopodial membrane trafficking. These studies represent the first in vivo screen for genes regulating invadopodia and demonstrate that invadopodia formation requires the integration of distinct cellular processes that are coordinated by an extracellular cue.
Assuntos
Proteínas de Caenorhabditis elegans/genética , Proteínas de Ciclo Celular/genética , Proteínas de Ligação ao GTP/genética , Inibidores de Dissociação do Nucleotídeo Guanina/genética , Neoplasias/genética , Podossomos/genética , Animais , Membrana Basal/crescimento & desenvolvimento , Caenorhabditis elegans/genética , Caenorhabditis elegans/crescimento & desenvolvimento , Proteínas de Caenorhabditis elegans/biossíntese , Proteínas de Ciclo Celular/biossíntese , Modelos Animais de Doenças , Matriz Extracelular/genética , Proteínas de Ligação ao GTP/biossíntese , Regulação da Expressão Gênica no Desenvolvimento , Inibidores de Dissociação do Nucleotídeo Guanina/biossíntese , Humanos , Neoplasias/patologia , Podossomos/patologia , Transdução de SinaisRESUMO
The construction of well-controllable in vitro models of physiological and pathological vascular endothelium remains a fundamental challenge in tissue engineering and drug development. Here, we present an approach for forming a synthetic endothelial extracellular matrix (ECM) that closely resembles that of the native structure by locally depositing basement membrane materials onto type 1 collagen nanofibers only in a region adjacent to the endothelial cell (EC) monolayer. Culturing the EC monolayer on this synthetic endothelial ECM remarkably enhanced its physiological properties, reducing its vascular permeability, and promoting a stabilized, quiescent phenotype. We demonstrated that the EC monolayer on the synthetic endothelial ECM neither creates non-physiological barriers to cell-cell or cell-ECM interactions, nor hinders molecular diffusion of growth factors and other molecules. The synthetic endothelial ECM and vascular endothelium on it may help us enter in a new phase of research in which various models of the biological barrier behavior can be tested experimentally.
Assuntos
Técnicas de Cultura de Células/métodos , Endotélio Vascular/crescimento & desenvolvimento , Matriz Extracelular/fisiologia , Engenharia Tecidual , Membrana Basal/citologia , Membrana Basal/crescimento & desenvolvimento , Adesão Celular/fisiologia , Colágeno Tipo I/química , Colágeno Tipo I/metabolismo , Endotélio Vascular/citologia , Humanos , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Microfluídica/métodos , Nanofibras/química , PermeabilidadeRESUMO
Granule cells (GCs) are the major glutamatergic neurons in the cerebellum, and GC axon formation is an initial step in establishing functional cerebellar circuits. In the zebrafish cerebellum, GCs can be classified into rostromedial and caudolateral groups, according to the locations of their somata in the corresponding cerebellar lobes. The axons of the GCs in the caudolateral lobes terminate on crest cells in the dorsal hindbrain, as well as forming en passant synapses with Purkinje cells in the cerebellum. In the zebrafish mutant shiomaneki, the caudolateral GCs extend aberrant axons. Positional cloning revealed that the shiomaneki (sio) gene locus encodes Col4a6, a subunit of type IV collagen, which, in a complex with Col4a5, is a basement membrane (BM) component. Both col4a5 and col4a6 mutants displayed similar abnormalities in the axogenesis of GCs and retinal ganglion cells (RGCs). Although type IV collagen is reported to control axon targeting by regulating the concentration gradient of an axonal guidance molecule Slit, Slit overexpression did not affect the GC axons. The structure of the BM surrounding the tectum and dorsal hindbrain was disorganized in the col4a5 and col4a6 mutants. Moreover, the abnormal axogenesis of the caudolateral GCs and the RGCs was coupled with aberrant BM structures in the type IV collagen mutants. The regrowth of GC axons after experimental ablation revealed that the original and newly formed axons displayed similar branching and extension abnormalities in the col4a6 mutants. These results collectively suggest that type IV collagen controls GC axon formation by regulating the integrity of the BM, which provides axons with the correct path to their targets.
Assuntos
Cerebelo/metabolismo , Colágeno Tipo IV/genética , Células Ganglionares da Retina/metabolismo , Peixe-Zebra/genética , Animais , Axônios/metabolismo , Membrana Basal/crescimento & desenvolvimento , Membrana Basal/metabolismo , Cerebelo/crescimento & desenvolvimento , Colágeno Tipo IV/metabolismo , Matriz Extracelular/genética , Matriz Extracelular/metabolismo , Proteínas do Tecido Nervoso/genética , Células de Purkinje/metabolismo , Peixe-Zebra/crescimento & desenvolvimento , Proteínas de Peixe-Zebra/genéticaRESUMO
The Drosophila Air Sac Primordium (ASP) has emerged as an important structure where cellular, genetic and molecular events responsible for invasive behavior and branching morphogenesis can be studied. In this report we present data which demonstrate that a Cathepsin-L encoded by the gene CP1 in Drosophila is necessary for invasive behavior during ASP development. We find that CP1 is expressed in ASP and knockdown of CP1 results in suppression of migratory and invasive behavior observed during ASP development. We further show that CP1 possibly regulates invasive behavior by promoting degradation of Basement Membrane. Our data provide clues to the possible role of Cathepsin L in human lung development and tumor invasion, especially, given the similarities between human lung and Drosophila ASP development.