RESUMO
Purpose: To test whether mice with microfibril deficiency due to the Tsk mutation of fibrillin-1 (Fbn1Tsk/+) have increased susceptibility to pressure-induced retinal ganglion cell (RGC) degeneration. Methods: Intraocular pressure (IOP) elevation was induced in Fbn1Tsk/+ and wild type (wt) mice by injecting microbeads into the anterior chamber. Mice were then followed up for four months, with IOP measurements every three to six days. Retinas were stained for Brn3a to determine RGC number. Optic nerve cross-sections were stained with p-phenylene diamine to determine nerve area, axon number, and caliber and thickness of the pia mater. Results: Microbead injection induced significant IOP elevation that was significantly less for Fbn1Tsk/+ mice compared with wt. The optic nerves and optic nerve axons were larger, and the elastic fiber-rich pia mater was thinner in Fbn1Tsk/+ mice. Microbead injection resulted in reduced optic nerve size, thicker pia mater, and a slight decrease in axon size. Fbn1Tsk/+ mice had significantly greater loss of RGCs and optic nerve axons compared with wt (14.8% vs. 5.8%, P = 0.002, and 17.0% vs. 7.5%, P = 0.002, respectively). Conclusions: Fbn1Tsk/+mice had altered optic nerve structure as indicated by larger optic nerves, larger optic nerve axons and thinner pia mater, consistent with our previous findings. Despite lower IOP elevation, Fbn1Tsk/+mice had greater loss of RGCs and optic nerve axons, suggesting increased susceptibility to IOP-induced optic nerve degeneration in microfibril-deficient mice.
Assuntos
Glaucoma/patologia , Microfibrilas/fisiologia , Células Ganglionares da Retina/patologia , Animais , Suscetibilidade a Doenças/patologia , Feminino , Fibrilina-1/genética , Glaucoma/complicações , Pressão Intraocular , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Microfibrilas/genética , Nervo Óptico/patologia , Retina/patologiaRESUMO
A crucial question in developmental biology is how cell growth is coordinated in living tissue to generate complex and reproducible shapes. We address this issue here in plants, where stiff extracellular walls prevent cell migration and morphogenesis mostly results from growth driven by turgor pressure. How cells grow in response to pressure partly depends on the mechanical properties of their walls, which are generally heterogeneous, anisotropic and dynamic. The active control of these properties is therefore a cornerstone of plant morphogenesis. Here, we focus on wall stiffness, which is under the control of both molecular and mechanical signaling. Indeed, in plant tissues, the balance between turgor and cell wall elasticity generates a tissue-wide stress field. Within cells, mechano-sensitive structures, such as cortical microtubules, adapt their behavior accordingly and locally influence cell wall remodeling dynamics. To fully apprehend the properties of this feedback loop, modeling approaches are indispensable. To that end, several modeling tools in the form of virtual tissues have been developed. However, these models often relate mechanical stress and cell wall stiffness in relatively abstract manners, where the molecular specificities of the various actors are not fully captured. In this paper, we propose to refine this approach by including parsimonious biochemical and biomechanical properties of the main molecular actors involved. Through a coarse-grained approach and through finite element simulations, we study the role of stress-sensing microtubules on organ-scale mechanics.
Assuntos
Parede Celular/fisiologia , Modelos Biológicos , Células Vegetais/fisiologia , Anisotropia , Fenômenos Biomecânicos , Celulose/metabolismo , Simulação por Computador , Elasticidade , Análise de Elementos Finitos , Conceitos Matemáticos , Mecanotransdução Celular/fisiologia , Microfibrilas/fisiologia , Microtúbulos/fisiologia , Desenvolvimento Vegetal/fisiologia , Estresse Mecânico , Interface Usuário-ComputadorRESUMO
MAIN CONCLUSION: CAD-deficient poplars enabled studying the influence of altered lignin composition on mechanical properties. Severe alterations in lignin composition did not influence the mechanical properties. Wood represents a hierarchical fiber-composite material with excellent mechanical properties. Despite its wide use and versatility, its mechanical behavior has not been entirely understood. It has especially been challenging to unravel the mechanical function of the cell wall matrix. Lignin engineering has been a useful tool to increase the knowledge on the mechanical function of lignin as it allows for modifications of lignin content and composition and the subsequent studying of the mechanical properties of these transgenics. Hereby, in most cases, both lignin composition and content are altered and the specific influence of lignin composition has hardly been revealed. Here, we have performed a comprehensive micromechanical, structural, and spectroscopic analysis on xylem strips of transgenic poplar plants, which are downregulated for cinnamyl alcohol dehydrogenase (CAD) by a hairpin-RNA-mediated silencing approach. All parameters were evaluated on the same samples. Raman microscopy revealed that the lignin of the hpCAD poplars was significantly enriched in aldehydes and reduced in the (relative) amount of G-units. FTIR spectra indicated pronounced changes in lignin composition, whereas lignin content was not significantly changed between WT and the hpCAD poplars. Microfibril angles were in the range of 18°-24° and were not significantly different between WT and transgenics. No significant changes were observed in mechanical properties, such as tensile stiffness, ultimate stress, and yield stress. The specific findings on hpCAD poplar allowed studying the specific influence of lignin composition on mechanics. It can be concluded that the changes in lignin composition in hpCAD poplars did not affect the micromechanical tensile properties.
Assuntos
Oxirredutases do Álcool/deficiência , Lignina/fisiologia , Populus/fisiologia , Lignina/metabolismo , Microfibrilas/metabolismo , Microfibrilas/fisiologia , Populus/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier , Análise Espectral Raman , Resistência à Tração , Difração de Raios XRESUMO
The growing plant cell wall is commonly considered to be a fibre-reinforced structure whose strength, extensibility and anisotropy depend on the orientation of crystalline cellulose microfibrils, their bonding to the polysaccharide matrix and matrix viscoelasticity1-4. Structural reinforcement of the wall by stiff cellulose microfibrils is central to contemporary models of plant growth, mechanics and meristem dynamics4-12. Although passive microfibril reorientation during wall extension has been inferred from theory and from bulk measurements13-15, nanometre-scale movements of individual microfibrils have not been directly observed. Here we combined nanometre-scale imaging of wet cell walls by atomic force microscopy (AFM) with a stretching device and endoglucanase treatment that induces wall stress relaxation and creep, mimicking wall behaviours during cell growth. Microfibril movements during forced mechanical extensions differ from those during creep of the enzymatically loosened wall. In addition to passive angular reorientation, we observed a diverse repertoire of microfibril movements that reveal the spatial scale of molecular connections between microfibrils. Our results show that wall loosening alters microfibril connectivity, enabling microfibril dynamics not seen during mechanical stretch. These insights into microfibril movements and connectivities need to be incorporated into refined models of plant cell wall structure, growth and morphogenesis.
Assuntos
Parede Celular/fisiologia , Celulose/química , Microfibrilas/fisiologia , Cebolas/fisiologia , Microscopia de Força AtômicaRESUMO
Bamboo, a fast-growing grass, has a higher strength-to-weight ratio than steel and concrete. The unique properties of bamboo come from the natural composite structure of fibers that consists mainly of cellulose microfibrils in a matrix of intertwined hemicellulose and lignin called lignin-carbohydrate complex (LCC). Here, we have used atomistic simulations to study the mechanical properties of and adhesive interactions between the materials in bamboo fibers. With this aim, we have developed molecular models of lignin, hemicellulose and LCC structures to study the elastic moduli and the adhesion energies between these materials and cellulose microfibril faces. Good agreement was observed between the simulation results and experimental data. It was also shown that the hemicellulose model has stronger mechanical properties than lignin while lignin exhibits greater tendency to adhere to cellulose microfibrils. The study suggests that the abundance of hydrogen bonds in hemicellulose chains is responsible for improving the mechanical behavior of LCC. The strong van der Waals forces between lignin molecules and cellulose microfibril is responsible for higher adhesion energy between LCC and cellulose microfibrils. We also found out that the amorphous regions of cellulose microfibrils are the weakest interfaces in bamboo fibrils. Hence, they determine the fibril strength.
Assuntos
Módulo de Elasticidade , Sasa/fisiologia , Estresse Mecânico , Resistência à Tração/fisiologia , Celulose/química , Matriz Extracelular , Lignina/química , Microfibrilas/fisiologia , Microfibrilas/ultraestrutura , Modelos Moleculares , Polissacarídeos/química , Sasa/genéticaRESUMO
The microfibril-associated glycoproteins MAGP-1 and MAGP-2 are extracellular matrix proteins that interact with fibrillin to influence microfibril function. The two proteins are related through a 60 amino acid matrix-binding domain but their sequences differ outside of this region. A distinguishing feature of both proteins is their ability to interact with TGFß family growth factors, Notch and Notch ligands, and multiple elastic fiber proteins. MAGP-2 can also interact with αvß3 integrins via a RGD sequence that is not found in MAGP-1. Morpholino knockdown of MAGP-1 expression in zebrafish resulted in abnormal vessel wall architecture and altered vascular network formation. In the mouse, MAGP-1 deficiency had little effect on elastic fibers in blood vessels and lung but resulted in numerous unexpected phenotypes including bone abnormalities, hematopoietic changes, increased fat deposition, diabetes, impaired wound repair, and a bleeding diathesis. Inactivation of the gene for MAGP-2 in mice produced a neutropenia yet had minimal effects on bone or adipose homeostasis. Double knockouts had phenotypes characteristic of each individual knockout as well as several additional traits only seen when both genes are inactivated. A common mechanism underlying all of the traits associated with the knockout phenotypes is altered TGFß signaling. This review summarizes our current understanding of the function of the MAGPs and discusses ideas related to their role in growth factor regulation.
Assuntos
Proteínas Contráteis/fisiologia , Proteínas da Matriz Extracelular/fisiologia , Microfibrilas/fisiologia , Processamento Alternativo , Sequência de Aminoácidos , Animais , Desenvolvimento Ósseo , Fibrilinas , Expressão Gênica , Glicoproteínas/fisiologia , Hematopoese , Humanos , Proteínas dos Microfilamentos/fisiologia , Dados de Sequência Molecular , Processamento de Proteína Pós-Traducional , Fatores de Processamento de RNA , CicatrizaçãoRESUMO
The fibrillins, large extracellular matrix molecules, are polymerized to form "microfibrils." The fibrillin microfibril scaffold is populated by microfibril-associated proteins and by growth factors, which are likely to be latent. The scaffold, associated proteins, and bound growth factors, together with cellular receptors that can sense the microfibril matrix, constitute the fibrillin microenvironment. Activation of TGFß signaling is associated with the Marfan syndrome, which is caused by mutations in fibrillin-1. Today we know that mutations in fibrillin-1 cause the Marfan syndrome as well as Weill-Marchesani syndrome (and other acromelic dysplasias) and result in opposite clinical phenotypes: tall or short stature; arachnodactyly or brachydactyly; joint hypermobility or stiff joints; hypomuscularity or hypermuscularity. We also know that these different syndromes are associated with different structural abnormalities in the fibrillin microfibril scaffold and perhaps with specific cellular receptors (mechanosensors). How does the microenvironment, framed by the microfibril scaffold and populated by latent growth factors, work? We must await future investigations for the molecular and cellular mechanisms that will answer this question. However, today we can appreciate the importance of the fibrillin microfibril niche as a contextual environment for growth factor signaling and potentially for mechanosensation.
Assuntos
Microfibrilas/fisiologia , Proteínas dos Microfilamentos/fisiologia , Animais , Proteínas Morfogenéticas Ósseas/fisiologia , Matriz Extracelular/fisiologia , Fibrilina-1 , Fibrilinas , Humanos , Síndrome de Marfan/genética , Síndrome de Marfan/metabolismo , Síndrome de Marfan/patologia , Mecanotransdução Celular , Fator de Crescimento Transformador beta/fisiologiaRESUMO
Elastic tissue was first described well over a hundred years ago and has since been identified in nearly every part of the body. In this review, we examine elastic tissue in the corneal stroma with some mention of other ocular structures which have been more thoroughly described in the past. True elastic fibers consist of an elastin core surrounded by fibrillin microfibrils. However, the presence of elastin fibers is not a requirement and some elastic tissue is comprised of non-elastin-containing bundles of microfibrils. Fibers containing a higher relative amount of elastin are associated with greater elasticity and those without elastin, with structural support. Recently it has been shown that the microfibrils, not only serve mechanical roles, but are also involved in cell signaling through force transduction and the release of TGF-ß. A well characterized example of elastin-free microfibril bundles (EFMBs) is found in the ciliary zonules which suspend the crystalline lens in the eye. Through contraction of the ciliary muscle they exert enough force to reshape the lens and thereby change its focal point. It is believed that the molecules comprising these fibers do not turn-over and yet retain their tensile strength for the life of the animal. The mechanical properties of the cornea (strength, elasticity, resiliency) would suggest that EFMBs are present there as well. However, many authors have reported that, although present during embryonic and early postnatal development, EFMBs are generally not present in adults. Serial-block-face imaging with a scanning electron microscope enabled 3D reconstruction of elements in murine corneas. Among these elements were found fibers that formed an extensive network throughout the cornea. In single sections these fibers appeared as electron dense patches. Transmission electron microscopy provided additional detail of these patches and showed them to be composed of fibrils (â¼10 nm diameter). Immunogold evidence clearly identified these fibrils as fibrillin EFMBs and EFMBs were also observed with TEM (without immunogold) in adult mammals of several species. Evidence of the presence of EFMBs in adult corneas will hopefully pique an interest in further studies that will ultimately improve our understanding of the cornea's biomechanical properties and its capacity to repair.
Assuntos
Substância Própria/ultraestrutura , Elastina/análise , Microfibrilas/ultraestrutura , Animais , Fibrilinas , Humanos , Imageamento Tridimensional/métodos , Imuno-Histoquímica , Microfibrilas/fisiologia , Proteínas dos Microfilamentos/análise , Microscopia Eletrônica de Varredura/métodos , Microscopia Eletrônica de TransmissãoRESUMO
X-ray diffraction technology was used to rapidly predict variation in microfibril angle (MFA) in Dendrocalamus fari- X-ray diffraction technology was used to rapidly predict variation in microfibril angle (MFA) in Dendrocalamus farinosus. The results show that there is little variation in MFA with bamboo age from 2a to 5e, and MFA of 3a is at the maximum with the value of 8.521 degrees. The average value of MFA of 2a or 3a is greater than 4a or 5a with absolute differences less than 0.10 degrees. MFA of base, middle and upper position respectively are 8.499 degrees, 8.497 degrees and 8.483 degrees with coefficient of variation about 5%. There is an increasing tendency from the periphery to the inner of bamboo culms. Variance analysis indicates that MFA is highly sensitive to radial position, but insensitive to bamboo age and longitudinal position. The correlation coefficient of longitudinal strength and modulus of elasticity (MOE) is 0.57. MFA was responsible for 35% and 43% of the variation found in longitudinal strength and MOE respectively, which means MFA has a certain extent effect on mechanical properties.
Assuntos
Microfibrilas/fisiologia , Poaceae/anatomia & histologia , Difração de Raios X , Análise de Variância , Resistência à TraçãoRESUMO
The main components of elastic fibers, elastin and fibrillin-containing microfibrils play a structural and mechanical role in the arteries and their essential function is to provide elasticity and resilience to the tissues. However, through control of the quiescent contractile phenotype of arterial smooth muscle cells, elastin also acts as an autocrine factor and, via the binding of 'latent transforming growth factor (TGF)-ß binding protein (LTBP) - latency-associated peptide (LAP) - TGF-ß' complexes, fibrillins regulate the activation and availability of TGF-ßs. These recent discoveries are detailed in this review.
Assuntos
Artérias/ultraestrutura , Tecido Elástico/fisiologia , Animais , Artérias/fisiologia , Comunicação Autócrina , Tecido Elástico/ultraestrutura , Elasticidade , Elastina/genética , Elastina/fisiologia , Fibrilinas , Glicoproteínas/fisiologia , Humanos , Camundongos , Camundongos Knockout , Microfibrilas/fisiologia , Microfibrilas/ultraestrutura , Proteínas dos Microfilamentos/fisiologia , Miócitos de Músculo Liso/citologia , Precursores de Proteínas/metabolismo , Processamento de Proteína Pós-Traducional , Fator de Crescimento Transformador beta/metabolismo , Resistência Vascular/fisiologia , Vasoconstrição/fisiologia , Síndrome de Williams/genética , Síndrome de Williams/metabolismo , Síndrome de Williams/patologiaRESUMO
Biomechanical forces can induce the transformation of fibronectin (Fn) from its compact structure to an extended fibrillar state. Adsorption of plasma proteins onto metallic surfaces may also influence their conformation. We used a cone-plate rheometer to investigate the effect of shear and stainless steel on conformational changes of Fn. In control experiments, cones grafted once or twice with polyethylene glycol were used. Plasma Fn was added at concentrations of 50 or 100 µg/ml to bovine serum albumin (BSA)- or Fn-coated plates and subsequently exposed to dynamic shear rates stepwise increasing from 50 to 5000 s-1 within 5 min and subsequently decreasing from 5000 to 50 s-1 within 5 min. The viscosity (mPa s) of Fn solutions was recorded over 10 min. Upon exposure to shear, the viscosity in the sample increased, suggesting conformational changes in Fn. Western blotting and densitometric analyses demonstrated that conformational changes of plasma Fn depended both on shear and protein concentration. However, there was no significant difference in fibril formation between BSA- or Fn-coated plates, suggesting that physical properties of stainless steel and biomechanical forces such as shear can affect the molecular structure of Fn. Our model may provide useful information of surface- and flow-induced alterations of plasma proteins.
Assuntos
Fibronectinas/sangue , Fibronectinas/química , Microfibrilas/química , Resistência ao Cisalhamento/fisiologia , Adulto , Velocidade do Fluxo Sanguíneo/fisiologia , Adesão Celular/fisiologia , Fibronectinas/isolamento & purificação , Humanos , Microfibrilas/fisiologia , Conformação Proteica , Soroalbumina Bovina/química , Aço InoxidávelRESUMO
The identification of bast fibre samples, in particular, bast fibres used in textiles, is an important issue in archaeology, criminology and other scientific fields. One of the characteristic features of bast fibres is their fibrillar orientation, referred to as Z- or S twist (or alternatively right- and left-handed fibres). An empirical test for determining the fibrillar orientation using polarized light microscopy has been known in the community for many years. It is referred to as the modified Herzog test or red plate test. The test has the reputation for never producing false results, but also for occasionally not working. However, so far, no proper justification has been provided in the literature that the 'no false results' assumption is really correct and it has also not been clear up till now, why the method sometimes does not work. In this paper, we present an analytical model for the modified Herzog test, which explains why the test never gives a false result. We also provide an explanation for why the Herzog test sometimes does not work: According to our model, the Herzog test will not work if none of the three distinct layers in the secondary cell wall is significantly thicker than the others.
Assuntos
Microfibrilas/fisiologia , Microscopia de Polarização/métodos , Têxteis/análise , Algoritmos , Parede Celular/metabolismo , PlantasRESUMO
The charging effects of microfibrils of sciatic nerve tissues due to electron irradiation are investigated using electron holography. The phenomenon that the charging effects are enhanced with an increase of electron intensity is visualized through direct observations of the electric potential distribution around the specimen. The electric potential at the surface of the specimen could be quantitatively evaluated by simulation, which takes into account the reference wave modulation due to the long-range electric field.
Assuntos
Eletricidade , Holografia/métodos , Microfibrilas/química , Microfibrilas/fisiologia , Nervo Isquiático/química , Nervo Isquiático/fisiologia , Animais , Camundongos , Camundongos Endogâmicos C57BLAssuntos
Tecido Elástico/fisiologia , Estudo de Associação Genômica Ampla , Microfibrilas/fisiologia , Estrias de Distensão/genética , Adulto , Tecido Elástico/patologia , Feminino , Humanos , Masculino , Microfibrilas/patologia , Pessoa de Meia-Idade , Gravidez , Pele/patologia , Estrias de Distensão/patologiaRESUMO
This brief text aims at illustrating the interactions between connective tissue fibers and cell cytoskeleton fibers. These two networks are connected by molecular bridges at the level of the cell membrane of the cells of the connective and vascular tissues, allowing functional adjustments across the two domains, but also the transduction of forces and tensions into a biochemical alphabet. The signaling between the cell kern and its environment, but equally the other way round, from the environment to the core of the cell, depends on it.
Assuntos
Citoesqueleto/fisiologia , Matriz Extracelular/fisiologia , Microfibrilas/fisiologia , HumanosRESUMO
Over previous decades and centuries, the difficulty encountered in the manner in which the tissue of our bodies is organised, and structured, is clearly explained by the impossibility of exploring it in detail. Since the creation of the microscope, the perception of the basic unity, which is the cell, has been essential in understanding the functioning of reproduction and of transmission, but has not been able to explain the notion of form; since the cells are not everywhere and are not distributed in an apparently balanced manner. The problems that remain are those of form and volume and also of connection. The concept of multifibrillar architecture, shaping the interfibrillar microvolumes in space, represents a solution to all these questions. The architectural structures revealed, made up of fibres, fibrils and microfibrils, from the mesoscopic to the microscopic level, provide the concept of a living form with structural rationalism that permits the association of psychochemical molecular biodynamics and quantum physics: the form can thus be described and interpreted, and a true structural ontology is elaborated from a basic functional unity, which is the microvacuole, the intra and interfibrillar volume of the fractal organisation, and the chaotic distribution. Naturally, new, less linear, less conclusive, and less specific concepts will be implied by this ontology, leading one to believe that the emergence of life takes place under submission to forces that the original form will have imposed and oriented the adaptive finality.
Assuntos
Fractais , Metafísica , Microfibrilas/fisiologia , Morfogênese/fisiologia , HumanosRESUMO
Large arteries allow the vascular system to be more than a simple route in which the blood circulates within the organism. The elastic fibers present in the wall endow these vessels with elasticity and are responsible for the smoothing of the blood pressure and flow, which are delivered discontinuously by the heart. This function is very important to ensure appropriate hemodynamics. Elastic fibers are composed of elastin (90%) and fibrillin-rich microfibrils (10%) which provide the vessels with elasticity and are also signals able to bind to relatively specific cell membrane receptors. Stimulation of the high affinity elastin receptor by elastin peptides or tropoelastin--the elastin precursor--triggers an increase in intracellular free calcium in vascular cells, especially endothelial cells, associated with attachment, migration or proliferation. Similar effects of the stimulation of endothelial cells by microfibrils or fibrillin-1 fragments, which bind to integrins, have been demonstrated. This dual function--mechanical and in signaling--makes the elastic fibers an important actor of the development and ageing processes taking place in blood vessels. An alteration of the elastin (Eln) or fibrillin (Fbn) gene products leads to severe genetic pathologies of the cardiovascular system, such as supravalvular aortic stenosis, or Williams Beuren syndrome--in which elastin deficiency induces aortic stenoses--or Marfan syndrome, in which on the contrary fibrillin-1 deficiency promotes the appearance of aortic aneurysms. Genetically-engineered mouse models of these pathologies (such as Eln+/- mice and Fbn-1+/mgΔ mice, Eln+/-Fbn-1+/- mice) have permitted a better understanding of the pathogenesis of these syndromes. In particular, it has been shown that elastin and fibrillin-1 roles can be complementary in some aspects, while they can be opposed in some other situations. For instance, the double heterozygosity in elastin and fibrillin-1 leads to increased arterial wall stress--compared to the level induced by one of these two deficiencies alone--while the decrease in diameter induced by Eln deficiency is partly compensated by an additional deficiency in Fbn-1. Also, it is now clear that early modifications of elastin or fibrillin-1 availability can alter the normal signaling action of these proteins and lead to long term modifications of the vascular physiology and ageing processes.
Assuntos
Vasos Sanguíneos/crescimento & desenvolvimento , Senescência Celular/fisiologia , Elastina/fisiologia , Microfibrilas/fisiologia , Animais , Vasos Sanguíneos/metabolismo , Senescência Celular/genética , Elastina/genética , Elastina/metabolismo , Fibrilina-1 , Fibrilinas , Humanos , Camundongos , Camundongos Knockout , Microfibrilas/genética , Microfibrilas/metabolismo , Proteínas dos Microfilamentos/genética , Proteínas dos Microfilamentos/metabolismo , Proteínas dos Microfilamentos/fisiologia , Modelos Biológicos , Neovascularização Fisiológica/genética , Neovascularização Fisiológica/fisiologia , Transdução de Sinais/genética , Transdução de Sinais/fisiologiaRESUMO
The biomechanical character of the periodontal ligament (PDL) is crucial in its response to functional and orthodontic forces. Collagen has been the primary subject of investigations in this field. Several studies, however, indicate that oxytalan fibres, which belong to the elastic fibre family, also contribute to the biomechanical character and behaviour of the PDL. In order to elucidate this, we have evaluated the available literature on the oxytalan fibre network within the PDL and supra-alveolar tissues with respect to development, morphology and distribution, and response to mechanical stimulation. To this end, we have combined the classical histological studies with more recent in vitro studies. Oxytalan fibres develop simultaneously with the root and the vascular system within the PDL. A close association between oxytalan fibres and the vascular system also remains later in life, suggesting a role in vascular support. Mechanical loading of the PDL, through orthodontic force application, appears to induce an increase in the number, size, and length of oxytalan fibres. In line with this, in vitro stretching of PDL fibroblasts (PDLFs) results in an increased production of fibrillin, a major structural component of the microfibrils that make up oxytalan fibres. The available data suggest a mechanical function for oxytalan, but to date experimental data are limited. Further research is required to clarify their exact mechanical function and possible role in orthodontic tooth movement.