RESUMO
The biology of extinct animals is usually reconstructed from external morphological characters and comparison with present-day analogues. Internal soft organs are very rarely preserved in fossils and require high-tech approaches for visualization. Here, we report the internal anatomy of a female and male of the ~ 162 Myr-old lobster Eryma ventrosum from the Jurassic La Voulte-sur-Rhône Konservat-Lagerstätte in France using X-ray synchrotron tomography. The Erymidae is an extinct, species-rich, widespread and ecologically important Mesozoic family of decapod crustaceans. Our investigation revealed the anatomy of the locomotory, respiratory, circulatory, excretory, digestive, nervous and sensory, and reproductive systems at a resolution resembling low-magnification histology. Particularly notable is the detailed preservation of the small brain and the fragile hepatopancreas, the main metabolic organ of decapods that decays rapidly post-mortem. The remarkable preservation shows that the internal anatomy of Eryma ventrosum is closer to that of Nephropidae (clawed lobsters) than Astacidae (freshwater crayfish), their closest living relatives based on skeletal morphology. The microanatomy of the gonads and hepatopancreas indicates that the two specimens investigated were a young, well-nourished female and male prior to sexual maturity. The analysis of the soft anatomy reveals remarkable conservatism over 160 Myr and offers new insights into feeding, reproduction, life history and lifestyle of an important component of the macrozoobenthos of Middle Jurassic seas.
Assuntos
Fósseis , Animais , Feminino , Fósseis/anatomia & histologia , Masculino , Decápodes/anatomia & histologia , França , Nephropidae/anatomia & histologiaRESUMO
Crustacean eggs are rare in the fossil record. Here we report the exquisite preservation of a fossil polychelidan embedded within an unbroken nodule from the Middle Jurassic La Voulte-sur-Rhône Lagerstätte (France) and found with hundreds of eggs attached to the pleon. This specimen belongs to a new species, Palaeopolycheles nantosueltae sp. nov. and offers unique clues to discuss the evolution of brooding behaviour in polychelidan lobsters. In contrast to their development, which now relies on a long-lived planktic larval stage that probably did not exist in the early evolutionary steps of the group, the brood size of polychelidan lobsters seems to have remained unchanged and comparatively small since the Jurassic. This finding is at odds with reproductive strategies in other lobster groups, in which a long-lived planktic larval stage is associated with a large brood size.
Assuntos
Nephropidae/classificação , Óvulo/química , Animais , Evolução Biológica , Fósseis/anatomia & histologia , Fósseis/história , França , História Antiga , Nephropidae/anatomia & histologia , Nephropidae/genética , Nephropidae/crescimento & desenvolvimento , Óvulo/classificação , Óvulo/crescimento & desenvolvimento , PaleontologiaRESUMO
Light microscopy studies of the female American lobster Homarus americanus reproductive system are essentially nonexistent or outdated. Based on samples taken in the spring, summer, and autumn from the southern Gulf of St. Lawrence between 1994 and 2014, and using a combination of histological and scanning electron microscope techniques, we propose an ovarian cycle with 10 stages, identifying for the first time a recovery stage. Also, an atypical resorption stage, characterized by massive reabsorption of mature oocytes, is occasionally observed during summer months. The oviducts are composed of connective tissue (elastic and collagen fibers) with no muscle or secretory activities. Their epithelium shows a cyclic pattern and phagocytosis activities linked to spawning. Although the role of the seminal receptacle is to store and protect semen, free spermatozoa (i.e., without the spermatophoric wall and the acellular gelatinous substance that constitute the semen) were also observed in its posteriolateral grooves immediately prior to spawning, which is consistent with an external fertilization mechanism at the seminal receptacle. Unexpectedly, free spermatozoa were observed externally near two pore-like structures located on the gonopore's operculum, not at the seminal receptacle, after spawning; hence, more work is needed to fully understand the fertilization mechanism for the American lobster.
Assuntos
Genitália Feminina/anatomia & histologia , Genitália Feminina/fisiologia , Nephropidae/anatomia & histologia , Nephropidae/fisiologia , Animais , Feminino , Genitália Feminina/ultraestrutura , Nephropidae/ultraestrutura , Oogênese , Ovário/citologia , Ovário/embriologiaRESUMO
Despite supporting a valuable fishery, the reproductive system of the male American lobster (Homarus americanus) is poorly understood. The elongated H-shaped testis is responsible for spermatogenesis and is composed of follicles, a common collecting duct with interlaced scattered striated muscles, and a serosa as an external wall. Sertoli cells are associated with the spermatogenesis that produces spermatozoa, which are transferred to the collecting duct through a temporary passageway. Spermatogenesis is asynchronous between follicles and occurs on a continuous basis. The anterior and posterior lobes of the testes are independent and connect to the vasa deferentia through the Y-shaped collecting tubules that have a different cell anatomy and function than the two organs they connect. The vas deferens is divided into four regions. Spermatophores, produced in the proximal vas deferens, are packets of spermatozoa encapsulated in a single layer-the spermatophoric wall, which is composed of mucopolysaccharide acid. Large dense ovoid granules and the seminal fluid, composed of acidic sulfated mucosubstances, are secreted in the median vas deferens. Spermatophores within these secreted substances (i.e., semen) are stored in the distal vas deferens that, with the spermiduct (last region of the vas deferens), is responsible for the extrusion of the semen by striated muscle contractions. Smooth muscles suggest a peristaltic movement of the spermatophores within the vas deferens. Finally, the gonopores and the first pair of pleopods (i.e., gonopod) move the semen to the female seminal receptacle during copulation.
Assuntos
Genitália Masculina/anatomia & histologia , Genitália Masculina/fisiologia , Nephropidae/anatomia & histologia , Nephropidae/fisiologia , Animais , Masculino , Espermatogênese , Espermatogônias/citologia , Espermatogônias/ultraestrutura , Testículo/anatomia & histologia , Testículo/citologia , Testículo/ultraestrutura , Ducto Deferente/anatomia & histologia , Ducto Deferente/citologia , Ducto Deferente/ultraestruturaRESUMO
The work is to investigate the relationships between the microstructures and mechanical behaviors of lobster cuticles and reveal the inner mechanisms of the anisotropic mechanical properties of the cuticles and give the helpful guidance for the design of high-performance man-made composites. First, the tensile mechanical properties of the longitudinal and transverse specimens of the cuticles of American lobsters were tested with a mechanical-testing instrument. It is was found that the fracture strength and elastic modulus of the longitudinal specimens are distinctly larger than those of the transverse specimens. Then, the microstructural characteristics of the fracture surfaces of the specimens were observed with scanning electron microscope. It was observed that the pore canals in the cuticles are elliptic and their orientations are along the longitudinal orientation of the cuticles. Furthermore, the stresses and micro-damage of the longitudinal and transverse specimens were calculated with the rule of progressive damage by finite element method. It was revealed that the shape and orientation of the pore canals in the cuticles give rise to the anisotropic mechanical property of the cuticles and ensure that the cuticles possess the largest fracture strength and elastic modulus along their largest main-stress orientation.
Assuntos
Exoesqueleto/fisiologia , Fenômenos Biomecânicos/fisiologia , Nephropidae/anatomia & histologia , Nephropidae/fisiologia , Estresse Mecânico , Animais , Módulo de Elasticidade , Microscopia Eletrônica de Varredura , Resistência à TraçãoRESUMO
The crustacean stomatogastric nervous system (STNS) is a well-known model for investigating neuropeptidergic control of rhythmic behavior. Among the peptides known to modulate the STNS are the C-type allatostatins (AST-Cs). In the lobster, Homarus americanus, three AST-Cs are known. Two of these, pQIRYHQCYFNPISCF (AST-C I) and GNGDGRLYWRCYFNAVSCF (AST-C III), have non-amidated C-termini, while the third, SYWKQCAFNAVSCFamide (AST-C II), is C-terminally amidated. Here, antibodies were generated against one of the non-amidated peptides (AST-C I) and against the amidated isoform (AST-C II). Specificity tests show that the AST-C I antibody cross-reacts with both AST-C I and AST-C III, but not AST-C II; the AST-C II antibody does not cross-react with either non-amidated peptide. Wholemount immunohistochemistry shows that both subclasses (non-amidated and amidated) of AST-C are distributed throughout the lobster STNS. Specifically, the antibody that cross-reacts with the two non-amidated peptides labels neuropil in the CoGs and the stomatogastric ganglion (STG), axons in the superior esophageal (son) and stomatogastric (stn) nerves, and ~ 14 somata in each commissural ganglion (CoG). The AST-C II-specific antibody labels neuropil in the CoGs, STG and at the junction of the sons and stn, axons in the sons and stn, ~ 42 somata in each CoG, and two somata in the STG. Double immunolabeling shows that, except for one soma in each CoG, the non-amidated and amidated peptides are present in distinct sets of neuronal profiles. The differential distributions of the two AST-C subclasses suggest that the two peptide groups are likely to serve different modulatory roles in the lobster STNS.
Assuntos
Sistema Digestório/citologia , Sistema Digestório/inervação , Gânglios dos Invertebrados/metabolismo , Neuropeptídeos/metabolismo , Animais , Nephropidae/anatomia & histologiaRESUMO
Gastroliths are highly calcified structures formed in the cardiac stomach wall of crustaceans for the temporary storage of amorphous CaCO3 (ACC). The gastrolithic ACC is stabilized by the presence of biomolecules, and represents a novel model for research into biomineralization. For the first time, an in vitro biomimetic retrosynthesis of scaffolds of gastrolithic matrices with CaCO3 is presented. With the help of synthetic polyacrylic (PAA) and phytic (PA) acids, amorphous precursor particles were stabilized in double (DD) and gas (GD) diffusion crystallization assays. The presence of these synthetic molecules as efficient inhibitors of nucleation and growth of CaCO3, and the use of biological gastrolith scaffolds as confined reaction environments determined the kinetics of crystallization, and controlled the morphogenesis of CaCO3. The formation of ACC particles was demonstrated and their crystallization was followed by light microscopy, scanning and transmission electron microscopy, and electron diffraction.
Assuntos
Biomimética/métodos , Calcificação Fisiológica , Carbonato de Cálcio/química , Nephropidae/anatomia & histologia , Animais , Cristalização , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de TransmissãoRESUMO
The genus Nephropsis Wood-Mason, 1872 has been reported from Brazil by Tavares (1998), Tavares & Young (2002), Silva et al. (2003), Dall´Occo et al. (2007) and Serejo et al. (2007), recording Nephropsis aculeata Smith, 1881, N. rosea Bate, 1888 and N. agassizii A. Milne-Edwards, 1880, the last of which occurs in both northeastern and southeastern of Brazil.
Assuntos
Nephropidae/classificação , Distribuição Animal , Estruturas Animais/anatomia & histologia , Estruturas Animais/crescimento & desenvolvimento , Animais , Tamanho Corporal , Brasil , Ecossistema , Feminino , Masculino , Nephropidae/anatomia & histologia , Nephropidae/crescimento & desenvolvimento , Tamanho do ÓrgãoRESUMO
The potential of natural products to prevent obesity have been investigated, with evidence to suggest that chitosan has anti-obesity effects. The current experiment investigated the anti-obesity potential of prawn shell derived chitosan on a range of variables relevant to obesity in a pig model. The two dietary treatment groups included in this 63 day study were: T1) basal diet and T2) basal diet plus 1000 ppm chitosan (n = 20 gilts per group (70 ± 0.90 kg). The parameter categories which were assessed included: performance, nutrient digestibility, serum leptin concentrations, nutrient transporter and digestive enzyme gene expression and gut microbial populations. Pigs offered chitosan had reduced feed intake and final body weight (P< 0.001), lower ileal digestibility of dry matter (DM), gross energy (GE) (P< 0.05) and reduced coefficient of apparent total tract digestibility (CATTD) of gross energy and nitrogen (P<0.05) when compared to the basal group. Fatty acid binding protein 2 (FABP2) gene expression was down-regulated in pigs offered chitosan (P = 0.05) relative to the basal diet. Serum leptin concentrations increased (P< 0.05) in animals offered the chitosan diet compared to pigs offered the basal diet. Fatness traits, back-fat depth (mm), fat content (kg), were significantly reduced while lean meat (%) was increased (P<0.05) in chitosan supplemented pigs. Pigs offered chitosan had decreased numbers of Firmicutes in the colon (P <0.05), and Lactobacillus spp. in both the caecum (P <0.05) and colon (P <0.001). Bifidobacteria populations were increased in the caecum of animals offered the chitosan diet (P <0.05). In conclusion, these findings suggest that prawn shell chitosan has potent anti-obesity/body weight control effects which are mediated through multiple biological systems in vivo.
Assuntos
Exoesqueleto/química , Quitosana/administração & dosagem , Trato Gastrointestinal/microbiologia , Microbiota/efeitos dos fármacos , Nephropidae/química , Obesidade/prevenção & controle , Animais , Peso Corporal/efeitos dos fármacos , Quitosana/farmacologia , Suplementos Nutricionais , Modelos Animais de Doenças , Ingestão de Alimentos/efeitos dos fármacos , Trato Gastrointestinal/efeitos dos fármacos , Leptina/sangue , Nephropidae/anatomia & histologia , SuínosRESUMO
The giant deep-sea lobster genus Acanthacaris Bate, 1888 is reported for the first time from Taiwan. The single specimen with a total length of 36 cm was collected near a cold seep off southwestern Taiwan at about 1300 m deep and identified as A. tenuimana Bate, 1888.
Assuntos
Nephropidae/classificação , Distribuição Animal , Estruturas Animais/anatomia & histologia , Estruturas Animais/crescimento & desenvolvimento , Animais , Tamanho Corporal , Ecossistema , Masculino , Nephropidae/anatomia & histologia , Nephropidae/crescimento & desenvolvimento , Tamanho do Órgão , TaiwanRESUMO
This study describes experiments carried out to examine effects of the antiparasitic drug teflubenzuron, used in delousing farmed salmon, on a non-target species, the European lobster (Homarus gammarus). Juvenile lobsters were fed two doses of teflubenzuron, 10 and 20mg/kg successively for 7 days corresponding to a standard medication of the fish (10mg/kg day) and twice the standard dose (20mg/kg day). Monitoring lasted 3 months to include at least one moulting period for all individuals. Cumulative mortality was higher in all replicates given medicated feed compared with the control group. Mean cumulative mortality for each dosing was 41 ± 13% for 10mg/kg and 38 ± 8% for 20mg/kg, i.e. no difference. Drug residue was analysed in all juveniles that died, in addition to 12 juveniles at day 8 and the first 12 surviving lobsters. A decline in concentration of teflubenzuron from over 8,000 ng/g (day 5) to 14 ng/g (day 70) was observed in the juveniles that died during the experiment. Twelve individuals that died contained 82 ng/g or less whereas the mean concentration in the first 12 lobsters that survived moulting was 152 ng/g. Following a single oral administration, the half-life of teflubenzuron in lobster was estimated to 3.4 days and the initial concentration (C0) to 515 ng/g at time t0. At the end of the study a considerable number of juvenile lobsters were observed with deformities in various organs; carapace, walking legs, cheliped, tail fan, abdomen and antenna. The occurrence of observed deformities varied from 0 to 15% in treated replicates and will most likely affect ability to locate and consume food (antenna, claw and walking legs), respiration (carapace) and ability to move/swim (walking legs, tail fan and abdomen). In total, the mortality and senescent damages were close to 50% in all replicates. Juveniles that survived medication without deformities however, moulted and increased in size at each moult equally well as the unmedicated controls.
Assuntos
Benzamidas/toxicidade , Nephropidae/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Animais , Antiparasitários/metabolismo , Antiparasitários/toxicidade , Benzamidas/metabolismo , Meia-Vida , Nephropidae/anatomia & histologia , Nephropidae/crescimento & desenvolvimento , Análise de Sobrevida , Poluentes Químicos da Água/metabolismoRESUMO
Most biological materials are nanocomposites characterized by a multi-level structural hierarchy. Particularly, the arthropod cuticle is a chitin-based composite material where the mechanical properties strongly depend on both molecular chitin/protein properties, and the structural arrangement of chitin-fibrils within the protein matrix. Here materials properties and structural organization of two types of cuticle from distantly related arthropods, the wandering spider Cupiennius salei and American lobster Homarus americanus were studied using nanoindentation and X-ray diffraction. The structural analysis of the two types of cuticle including the packing and alignment of chitin-fibrils is supported by Monte Carlo simulations of the experimental X-ray data, thereby regions of parallel and rotated fibril arrangement can be clearly distinguished. The tip of the spider fang which is used to inject venom into the prey was found to be considerably harder than the lobster carapace, while its stiffness is slightly lower.
Assuntos
Exoesqueleto/metabolismo , Proteínas de Artrópodes/metabolismo , Quitina/metabolismo , Nephropidae/fisiologia , Aranhas/fisiologia , Exoesqueleto/química , Animais , Nephropidae/anatomia & histologia , Picada de Aranha , Aranhas/anatomia & histologia , Difração de Raios XRESUMO
The digestive gland of crustacean is involved in various metabolic activities, including the synthesis and secretion of digestive enzymes that begin the process of food digestion, intracellular digestion and absorption of nutrients, storage of reserves, and disposal of waste products. It consists of two glandular lobes which extensively subdivide to form a complex of blind-ending tubules, whose size, surface area, and digestive cells are associated with intracellular digestion and the nutritional status of the organism. The aim of this paper was to study the morphology of the digestive gland in various lobster species and calculate the surface area of tubules, lumen and digestive cells (R-, F-, and B-cells) and their ratios to total tubule surface area. The similarity in ratios obtained in this study between individual lobsters suggests that the method developed in this study can be successfully applied to a range of species. This study describes a novel image processing algorithm for the automatic measurement of the hepatopancreas structure using stained cross sections of digestive gland tubules. The proposed new methodology could be used for studying the physiology and nutrient metabolism of lobsters and other crustaceans. The computer-aided analysis described in this paper is accurate for the quantitative assessment of the lobster's digestive gland structure.
Assuntos
Biometria/métodos , Processamento de Imagem Assistida por Computador/métodos , Nephropidae/anatomia & histologia , Algoritmos , Animais , Automação/métodos , Sistema Digestório/anatomia & histologia , Histocitoquímica/métodosRESUMO
Previous in vitro and in vivo studies showed that the frequency of rhythmic pyloric network activity in the lobster is modulated directly by oxygen partial pressure (PO(2)). We have extended these results by (1) increasing the period of exposure to low PO(2) and by (2) testing the sensitivity of the pyloric network to changes in PO(2) that are within the narrow range normally experienced by the lobster (1 to 6 kPa). We found that the pyloric network rhythm was indeed altered by changes in PO(2) within the range typically observed in vivo. Furthermore, a previous study showed that the lateral pyloric constrictor motor neuron (LP) contributes to the O(2) sensitivity of the pyloric network. Here, we expanded on this idea by testing the hypothesis that pyloric pacemaker neurons also contribute to pyloric O(2) sensitivity. A 2-h exposure to 1 kPa PO(2), which was twice the period used previously, decreased the frequency of an isolated group of pacemaker neurons, suggesting that changes in the rhythmogenic properties of these cells contribute to pyloric O(2) sensitivity during long-term near-anaerobic (anaerobic threshold, 0.7-1.2 kPa) conditions.
Assuntos
Limiar Anaeróbio , Relógios Biológicos , Sistema Digestório/inervação , Nephropidae/metabolismo , Neurônios/metabolismo , Oxigênio/metabolismo , Periodicidade , Potenciais de Ação , Animais , Nephropidae/anatomia & histologia , Rede Nervosa/metabolismo , Fatores de TempoRESUMO
The Norway lobster (Nephrops norvegicus) is economically important in Europe. However, apart from the female reproductive system, very little is known about its internal anatomy. This article focuses on studying the internal anatomy and ultrastructure of the male reproductive system. This system follows the general pattern found among decapod crustaceans, with several peculiarities. Testes are composed of lobular sperm ducts in which the spermatozoa are fully constituted. The spermatozoa present three lateral arms and a long acrosome, which gives a false appearance of flagellated spermatozoa. The two testes form a double H under the heart, and the vas deferens (VD) arise from each side at the posterior edge of the double H. The main characteristic of the VD is the presence of a sphincter in the enlarged area of the distal end of the middle VD. The MVD here shows an increase in musculature of the wall as compared to the VD, which regulates the passage of the sperm cord to the distal VD (DVD) and thence to the thelycum of the female. The wall of the spermatophore is formed in the distal part of the proximal VD, which surrounds the unique sperm cord present in the VD. Isolated spermatophores are not observed in the VD. The sperm cord is pinched off during copulation by the musculature of the DVD. Then, a portion of the sperm cord is transferred from each VD to form the isolated spermatophores. The wall of the spematophores and the spermatozoa that are observed inside the thelycum have the same morphology as those observed in the VD.
Assuntos
Nephropidae/anatomia & histologia , Acrossomo/ultraestrutura , Animais , Masculino , Nephropidae/ultraestrutura , Reprodução , Espermatogônias/ultraestrutura , Espermatozoides/fisiologia , Espermatozoides/ultraestrutura , Testículo/anatomia & histologia , Testículo/ultraestrutura , Sistema Urogenital/anatomia & histologia , Sistema Urogenital/ultraestrutura , Ducto Deferente/ultraestruturaRESUMO
Ectoparasitic copepods have been reported in a wide range of aquatic animals, including crustacean shellfish. However, with the exception of the salmon louse, Lepeophtheirus salmonis, our knowledge of such parasites in commercial species is rudimentary. The current study examines the morphology and pathology of the parasitic copepod, Nicothoë astaci (the 'lobster louse') in its host, the European lobster, Homarus gammarus. Lobsters were sampled from waters surrounding Lundy Island (Bristol Channel, UK) and all individuals collected were found to harbour female adult N. astaci in their gills, with a mean of 47·3 parasites/lobster. The majority of N. astaci were found in the basal region of pleurobranch gills. The parasite was found to attach to gill filaments via its oral sucker, maxillae and maxillipeds, and to feed on host haemolymph (blood) through a funnel-like feeding channel. It caused varying degrees of damage to the host gill, including occlusion of gill filaments and disruption to the vascular system in the central axis. Although there was evidence of extensive host response (haemocytic infiltration) to the parasite, it was displaced from the parasite attachment site and thus was observed in the central gill axis below. The region of gill filament immediately underlying the parasite feeding channel was devoid of such activity suggesting that the parasite interferes with the cellular defence and haemostatic mechanisms of the lobster in order to maintain invasion of the host.
Assuntos
Copépodes/fisiologia , Ectoparasitoses/parasitologia , Brânquias/parasitologia , Interações Hospedeiro-Parasita , Nephropidae/parasitologia , Frutos do Mar/parasitologia , Animais , Copépodes/ultraestrutura , Ectoparasitoses/imunologia , Ectoparasitoses/patologia , Amarelo de Eosina-(YS)/análise , Feminino , Brânquias/imunologia , Brânquias/ultraestrutura , Hematoxilina/análise , Hemócitos/citologia , Hemócitos/imunologia , Hemolinfa/citologia , Interações Hospedeiro-Parasita/imunologia , Masculino , Microscopia Confocal , Nephropidae/anatomia & histologia , Nephropidae/imunologia , Reino UnidoRESUMO
Recently, we proposed a hierarchical model for the elastic properties of mineralized lobster cuticle using (i) ab initio calculations for the chitin properties and (ii) hierarchical homogenization performed in a bottom-up order through all length scales. It has been found that the cuticle possesses nearly extremal, excellent mechanical properties in terms of stiffness that strongly depend on the overall mineral content and the specific microstructure of the mineral-protein matrix. In this study, we investigated how the overall cuticle properties changed when there are significant variations in the properties of the constituents (chitin, amorphous calcium carbonate (ACC), proteins), and the volume fractions of key structural elements such as chitin-protein fibers. It was found that the cuticle performance is very robust with respect to variations in the elastic properties of chitin and fiber proteins at a lower hierarchy level. At higher structural levels, variations of design parameters such as the volume fraction of the chitin-protein fibers have a significant influence on the cuticle performance. Furthermore, we observed that among the possible variations in the cuticle ingredients and volume fractions, the experimental data reflect an optimal use of the structural variations regarding the best possible performance for a given composition due to the smart hierarchical organization of the cuticle design.
Assuntos
Simulação de Dinâmica Molecular , Nephropidae/anatomia & histologia , Nephropidae/metabolismo , Teoria Quântica , Esqueleto , Animais , Fenômenos Biomecânicos , Quitina/química , Quitina/metabolismo , Módulo de Elasticidade , Minerais/metabolismo , Conformação Molecular , Nephropidae/fisiologia , Proteínas/química , Proteínas/metabolismo , Suporte de CargaRESUMO
Gelsolin was localized by immunoelectron microscopy in fast and slow cross-striated muscles of the lobster Homarus americanus. When ultrathin sections of the muscles were labelled with anti-gelsolin and a gold-conjugated second antibody, 90% of all gold particles in the myoplasm were detected on myofibrils, preferentially in the I-band and AI-region of the sarcomeres. Both the region of the H-zone (lacking thin filaments) and the Z-disc contained no or little gold label. Under physiological conditions, a close association of gelsolin with the thin filaments was observed for both muscle types. The preferential localization of particles in the I- and AI-region indicated that gelsolin was distributed randomly over the whole length of the thin filaments. Preincubation of muscle strips with Ringer solution containing 0.5 mM EGTA resulted in a significantly different distribution pattern; gold particles were now localized preferentially in the cell periphery close to the sarcolemma, with significantly decreased abundance in the centre of the cell. Compared with the muscle under physiological conditions, the number of gold particles over sarcomeric structures was significantly reduced. Thus, binding of gelsolin to the thin filaments is apparently reversible in vivo and depends on the presence of calcium ions. We assume a functional role for gelsolin in the actin turnover processes in invertebrate muscle systems.
Assuntos
Gelsolina/análise , Miofibrilas/química , Nephropidae/química , Actinas/análise , Actinas/metabolismo , Animais , Western Blotting , Cálcio/análise , Cálcio/metabolismo , Citoesqueleto/metabolismo , Eletroforese em Gel de Poliacrilamida , Gelsolina/imunologia , Gelsolina/ultraestrutura , Microscopia Imunoeletrônica , Miofibrilas/metabolismo , Miofibrilas/ultraestrutura , Nephropidae/anatomia & histologia , Nephropidae/ultraestrutura , Sarcômeros/química , Sarcômeros/metabolismo , Sarcômeros/ultraestruturaRESUMO
Unfolded epipodite isolated from American lobsters (Homarus americanus) acclimated to dilute seawater was mounted in an Ussing-type chamber for ion transport studies. The split epipodite is an electrically polarized, one-cell-layer epithelium supported with cuticle. Under open-circuit conditions, the transepithelial potential was -4.2+/-1.0 mV (N=38). In the short-circuited epithelium, the current averaged over all of the preparations was -185.4+/-20.2 A cm(-2) (N=38) with a high conductance of 55.2+/-11.4 mS cm(-2) (N=38), typical for a leaky epithelium. The Na:Cl absorptive flux ratio was 1:1.6; ion substitution experiments indicated that the transport of Na+ and Cl- is coupled. Basolateral application of the Cl- channel blockers 5-nitro-2-(3-phenylpropylamino) benzoate (NPPB) and niflumic acid (NFA) dose-dependently inhibited short-circuit current (ISC). Secretory K+ (Rb+) fluxes exceeded influxes and were inhibited by the Na+/K+-ATPase inhibitor ouabain and the K+ channel blocker cesium. Western blot analysis showed that Na+/K+-ATPase alpha-subunit protein was more highly expressed in the epipodite of lobsters acclimated to 20 p.p.t. compared with animals acclimated to seawater (34 p.p.t.). 3-Isobutyl-1-methyl-xanthine (IBMX) stimulated a negative ISC and enhanced apical secretory K+ flux. Basolateral application of NPPB inhibited JRbB-->A fluxes, suggesting the interaction of K+ channels with NPPB-sensitive Cl- channels. The results are summarized in a transport model, suggesting apical Na+/K+/2Cl- co-transport, a dominant apical K+-secreting channel and basolaterally located Cl- and K+ channels. This study represents the first comprehensive characterization of ion transport processes across the lobster epipodite epithelium and indeed in any tissue within the branchial cavity of the American lobster.