RESUMO
In this study, catalytic generation of nitric oxide by a copper(II) complex embedded within a poly(vinyl chloride) matrix in the presence of nitrite (source of nitric oxide) and ascorbic acid (reducing agent) was shown to effectively control the formation and dispersion of nitrifying bacteria biofilms. Amperometric measurements indicated increased and prolonged generation of nitric oxide with the addition of the copper complex when compared to that with nitrite and ascorbic acid alone. The effectiveness of the copper complex-nitrite-ascorbic acid system for biofilm control was quantified using protein analysis, which showed enhanced biofilm suppression when the copper complex was used in comparison to that with nitrite and ascorbic acid treatment alone. Confocal laser scanning microscopy (CLSM) and LIVE/DEAD staining revealed a reduction in cell surface coverage without a loss of viability with the copper complex and up to 5 mM of nitrite and ascorbic acid, suggesting that the nitric oxide generated from the system inhibits proliferation of the cells on surfaces. Induction of nitric oxide production by the copper complex system also triggered the dispersal of pre-established biofilms. However, the addition of a high concentration of nitrite and ascorbic acid to a pre-established biofilm induced bacterial membrane damage and strongly decreased the metabolic activity of planktonic and biofilm cells, as revealed by CLSM with LIVE/DEAD staining and intracellular adenosine triphosphate measurements, respectively. This study highlights the utility of the catalytic generation of nitric oxide for the long-term suppression and removal of nitrifying bacterial biofilms.
Assuntos
Complexos de Coordenação/química , Cobre/química , Óxido Nítrico/metabolismo , Cloreto de Polivinila/química , Ácido Ascórbico/química , Ácido Ascórbico/farmacologia , Biofilmes/efeitos dos fármacos , Catálise , Microscopia Confocal , Nitritos/química , Nitritos/farmacologia , Nitrosomonas europaea/fisiologia , Espectroscopia FotoeletrônicaRESUMO
Biofilms of the ammonia oxidizing bacterium Nitrosomonas europaea were cultivated to study microbial processes associated with ammonia oxidation in pure culture. We explored the hypothesis that the kinetic parameters of ammonia oxidation in N. europaea biofilms were in the range of those determined with batch suspended cells. Oxygen and pH microelectrodes were used to measure dissolved oxygen (DO) concentrations and pH above and inside biofilms and reactive transport modeling was performed to simulate the measured DO and pH profiles. A two dimensional (2-D) model was used to simulate advection parallel to the biofilm surface and diffusion through the overlying fluid while reaction and diffusion were simulated in the biofilm. Three experimental studies of microsensor measurements were performed with biofilms: i) NH3 concentrations near the Ksn value of 40 µM determined in suspended cell tests ii) Limited buffering capacity which resulted in a pH gradient within the biofilms and iii) NH3 concentrations well below the Ksn value. Very good fits to the DO concentration profiles both in the fluid above and in the biofilms were achieved using the 2-D model. The modeling study revealed that the half-saturation coefficient for NH3 in N. europaea biofilms was close to the value measured in suspended cells. However, the third study of biofilms with low availability of NH3 deviated from the model prediction. The model also predicted shifts in the DO profiles and the gradient in pH that resulted for the case of limited buffering capacity. The results illustrate the importance of incorporating both key transport and chemical processes in a biofilm reactive transport model.
Assuntos
Amônia/metabolismo , Biofilmes/crescimento & desenvolvimento , Nitrosomonas europaea/fisiologia , Meios de Cultura/química , Concentração de Íons de Hidrogênio , Modelos Estatísticos , Nitrosomonas europaea/crescimento & desenvolvimento , Nitrosomonas europaea/metabolismo , Oxirredução , Oxigênio/análiseRESUMO
Nitrosomonas europaea, a model ammonia oxidizing bacterium, was sensitive to both ionic silver (Ag(+)) and 20 nm citrate capped silver nanoparticles (AgNPs). AgNP toxicity has been previously shown to be primarily due to the dissolution of Ag(+). The rate of AgNP dissolution dramatically increased in test medium containing ammonium sulfate ((NH4)2SO4) and HEPES buffer compared to test medium containing either deionized water or HEPES buffer alone. The AgNP dissolution rates accelerated with increases in ammonia (NH3) concentrations either through increases in pH or through higher (NH4)2SO4 concentrations. Ammonia likely participated in the oxidation of the AgNP to form [Formula: see text] in solution leading to the observed increase in AgNP dissolution rates. AgNP toxicity was enhanced as NH3 concentrations increased. However, Ag(+) toxicity was constant at all NH3 concentrations tested. Therefore, it can be concluded that the increased AgNP toxicity was due to increased Ag(+) release and not due to a synergistic effect between NH3 and Ag(+). The results of this study may provide insights in the fate and toxicity of AgNPs in high NH3 environments including wastewater treatment plants, eutrophic waterways and alkaline environments.
Assuntos
Amônia/química , Nanopartículas Metálicas/toxicidade , Nitrosomonas europaea/efeitos dos fármacos , Prata/toxicidade , Poluentes Químicos da Água/toxicidade , Nanopartículas Metálicas/química , Nitrosomonas europaea/fisiologia , Prata/química , Poluentes Químicos da Água/químicaRESUMO
Ammonia oxidation by Nitrosomonas europaea, an ammonia oxidizing bacterium prevalent in wastewater treatment, is inhibited in the presence of phenol, due to interaction of the phenol with the ammonia monooxygenase enzyme. Suspended cells of N. europaea were cultured in batch reactors and continuous flow reactors at dilution rates of 0.01-0.2 d(-1). The rate of ammonia oxidation in the continuous cultures correlated to the dilution rate in the reactor. The batch and continuous cultures were exposed to 20 µM phenol and ammonia oxidation activity was measured by specific oxygen uptake rates (SOURs). Inhibition of NH3 oxidation by 20 µM phenol ranged from a 77% reduction of SOUR observed with suspended cells harvested during exponential growth, to 26% in biofilms. The extent of inhibition was correlated with ammonia oxidation rates in both suspended and biofilm cells, with greater percent inhibition observed with higher initial rates of NH3 oxidation. In biofilm grown cells, an increase in activity and phenol inhibition were both observed upon dispersing the biofilm cells into fresh, liquid medium. Under higher oxygen tension, an increase in the NO2(-) production of the biofilms was observed and biofilms were more susceptible to phenol inhibition. Dissolved oxygen microsensor measurements showed oxygen limited conditions existed in the biofilms. The ammonia oxidation rate was much lower in biofilms, which were less inhibited during phenol exposure. The results clearly indicate in both suspended and attached cells of N. europaea that a higher extent of phenol inhibition is positively correlated with a higher rate of NH3 oxidation (enzyme turnover).
Assuntos
Amônia/metabolismo , Técnicas de Cultura Celular por Lotes/métodos , Biofilmes/crescimento & desenvolvimento , Nitrosomonas europaea/efeitos dos fármacos , Nitrosomonas europaea/metabolismo , Fenol/farmacologia , Biofilmes/efeitos dos fármacos , Reatores Biológicos/microbiologia , Nitritos/metabolismo , Nitrosomonas europaea/fisiologia , Oxirredução/efeitos dos fármacos , Oxigênio/metabolismoRESUMO
The toxicity of the water associated fraction (WAF) of Alaska North Slope Crude oil (ANSC), Corexit 9500A and the dispersant enhanced WAF (DEWAF) of ANSC:Corexit 9500A mixtures were examined on the model ammonia oxidizing bacterium, Nitrosomonas europaea. Corexit 9500A was not toxic at environmentally relevant concentrations. Corexit 9500A greatly increased the toxicity of ANSC by increasing the chemical oxygen demand (COD) of the DEWAF. However, a majority of the DEWAF compounds were not toxic to N. europaea. Weathered WAF and DEWAF were not toxic to N. europaea even though their COD did not change compared to non-weathered controls, suggesting that toxicity was due to a small volatile fraction of the ANSC. The over-expression of the NE1545 gene, a marker for aromatic hydrocarbon exposure, in N. europaea cells exposed to WAF and DEWAF suggests that aromatic hydrocarbons are bioavailable to the cells and may play a role in the observed toxicity.
Assuntos
Lipídeos/toxicidade , Nitrosomonas europaea/efeitos dos fármacos , Petróleo/toxicidade , Tensoativos/toxicidade , Poluentes Químicos da Água/toxicidade , Alaska , Amônia/análise , Amônia/metabolismo , Nitrosomonas europaea/fisiologia , Poluentes Químicos da Água/análise , Tempo (Meteorologia)RESUMO
A suite of techniques was utilized to evaluate the correlation between biofilm physiology, fluid-induced shear stress, and detachment in hollow fiber membrane aerated bioreactors. Two monoculture species biofilms were grown on silicone fibers in a hollow fiber membrane aerated bioreactors (HfMBR) to assess detachment under laminar fluid flow conditions. Both physiology (biofilm thickness and roughness) and nutrient mass transport data indicated the presence of a steady state mature biofilm after 3 weeks of development. Surface shear stress proved to be an important parameter for predicting passive detachment for the two biofilms. The average shear stress at the surface of Nitrosomonas europaea biofilms (54.5 ± 3.2 mPa) was approximately 20% higher than for Pseudomonas aeruginosa biofilms (45.8 ± 7.7 mPa), resulting in higher biomass detachment. No significant difference in shear stress was measured between immature and mature biofilms of the same species. There was a significant difference in detached biomass for immature vs. mature biofilms in both species. However, there was no difference in detachment rate between the two species.
Assuntos
Biofilmes , Reatores Biológicos/microbiologia , Membranas Artificiais , Análise de Variância , Fenômenos Fisiológicos Bacterianos , Biotecnologia/instrumentação , Hidrodinâmica , Nitrosomonas europaea/fisiologia , Pseudomonas aeruginosa/fisiologia , Resistência ao Cisalhamento , Silicones/química , Estresse MecânicoRESUMO
In this study, Nitrosomonas europaea ATCC 19718 was exposed to silver nanoparticles (AgNPs) of different particle size (7±3 and 40±14nm) and different coatings (polyvinyl alcohol and adenosine triphosphate disodium). For all different AgNPs used in the study, large aggregates were gradually formed after addition of AgNPs into the media containing N. europaea. The scanning electron microscopy and energy dispersive X-ray spectroscopy of the microstructures suggested that bacterial cells and electrolytes had significant effects on AgNP aggregation. Size- and coating-dependent inhibition of ammonia oxidation by AgNPs was observed, and our analysis suggested that the inhibition was not only due to the released dissolved silver, but also the dispersity of AgNPs in the culture media. Electron microscopy images showed AgNPs could cause the damage of cell wall of N. europaea and make the nucleoids disintegrated and condensed next to cell membrane. Surface-enhanced Raman scattering signals also implied the damage of cell membrane caused by AgNPs. Further protein expression analysis revealed that AgNPs would inhibit important protein functions, including biosynthesis, gene expression, energy production and nitrification to further cause toxicity to N. europaea. Our findings explain the susceptibility of N. europaea to inhibition by AgNPs and the possible interaction between each other. Future research is needed to characterize these effects in more complex cultures and media such as activated sludge and wastewater.
Assuntos
Substâncias Perigosas/toxicidade , Nanopartículas Metálicas/toxicidade , Nitrosomonas europaea/efeitos dos fármacos , Prata/toxicidade , Nanopartículas Metálicas/química , Nanopartículas Metálicas/ultraestrutura , Microscopia Eletrônica de Varredura , Nitrificação , Nitrosomonas europaea/fisiologia , Prata/química , Análise Espectral RamanRESUMO
The ammonia monooxygenase of chemolithotrophic ammonia-oxidizing bacteria (AOB) catalyzes the first step in ammonia oxidation by converting ammonia to hydroxylamine. The monooxygenase of Nitrosomonas europaea is encoded by two nearly identical operon copies (amoCAB(1,2)). Several AOB, including N. europaea, also possess a divergent monocistronic copy of amoC (amoC(3)) of unknown function. Previous work suggested a possible functional role for amoC(3) as part of the σ(E) stress response regulon during the recovery of N. europaea from extended ammonia starvation, thus indicating its importance during the exit of cells from starvation. We here used global transcription analysis to show that expression of amoC(3) is part of a general poststarvation cellular response system in N. europaea. We also found that amoC(3) is required for an efficient response to some stress conditions, as deleting this gene impaired growth at elevated temperatures and recovery following starvation under high oxygen tensions. Deletion of the σ(32) global stress response regulator demonstrated that the heat shock regulon plays a significant role in mediating the recovery of N. europaea from starvation. These findings provide the first described phenotype associated with the divergent AmoC(3) subunit which appears to function as a stress-responsive subunit capable of maintaining ammonia oxidation activity under stress conditions. While this study was limited to starvation and heat shock, it is possible that the AmoC(3) subunit may be responsive to other membrane stressors (e.g., solvent or osmotic shocks) that are prevalent in the environments of AOB.
Assuntos
Amônia/metabolismo , Resposta ao Choque Térmico , Nitrosomonas europaea/fisiologia , Oxirredutases/metabolismo , Subunidades Proteicas/metabolismo , Mutação , Nitrosomonas europaea/genética , Nitrosomonas europaea/metabolismo , Análise de Sequência com Séries de Oligonucleotídeos , Óperon , Oxirredução , Oxirredutases/genética , Oxigênio/metabolismo , Subunidades Proteicas/genética , Transcrição Gênica , TranscriptomaRESUMO
Living hybrid materials that respond dynamically to their surrounding environment have important applications in bioreactors. Silica based sol-gels represent appealing matrix materials as they form a mesoporous biocompatible glass lattice that allows for nutrient diffusion while firmly encapsulating living cells. Despite progress in sol-gel cellular encapsulation technologies, current techniques typically form bulk materials and are unable to generate regular silica membranes over complex geometries for large-scale applications. We have developed a novel biomimetic encapsulation technique whereby endogenous extracellular matrix molecules facilitate formation of a cell surface specific biomineral layer. In this study, monoculture Pseudomonas aeruginosa and Nitrosomonas europaea biofilms are exposed to silica precursors under different acid conditions. Scanning electron microscopy (SEM) imaging and electron dispersive X-ray (EDX) elemental analysis revealed the presence of a thin silica layer covering the biofilm surface. Cell survival was confirmed 30 min, 30 days, and 90 days after encapsulation using confocal imaging with a membrane integrity assay and physiological flux measurements of oxygen, glucose, and NH 4âº. No statistical difference in viability, oxygen flux, or substrate flux was observed after encapsulation in silica glass. Shear induced biofilm detachment was assessed using a particle counter. Encapsulation significantly reduced detachment rate of the biofilms for over 30 days. The results of this study indicate that the thin regular silica membrane permits the diffusion of nutrients and cellular products, supporting continued cellular viability after biomineralization. This technique offers a means of controllably encapsulating biofilms over large surfaces and complex geometries. The generic deposition mechanism employed to form the silica matrix can be translated to a wide range of biological material and represents a platform encapsulation technology.
Assuntos
Biofilmes/crescimento & desenvolvimento , Nitrosomonas europaea/fisiologia , Pseudomonas aeruginosa/fisiologia , Dióxido de Silício/metabolismo , Nitrosomonas europaea/ultraestrutura , Porosidade , Pseudomonas aeruginosa/ultraestruturaRESUMO
The terrestrial biotic ligand model (BLM) for metal toxicity in soil postulates that metal toxicity depends on the free metal ion activity in solution and on ions competing for metal sorption to the biotic ligand. Unequivocal evidence for the BLM assumptions is most difficult to obtain for native soil microorganisms because the abiotic and biotic compartments cannot be experimentally separated. Here, we report copper (Cu) toxicity to a bioluminescent Nitrosomonas europaea reporter strain that was used in a solid phase-contact assay and in corresponding soil extracts and artificial soil solutions. The Cu(2+) ion activities that halve bioluminescence (EC50) in artificial solutions ranged 10(-5) to 10(-7) M and increased with increasing activities of H(+), Ca(2+) and Mg(2+) according to the BLM concept. The solution based Cu(2+) EC50 values of N. europaea in six contaminated soils ranged 2 × 10(-6) to 2 × 10(-9) M and these thresholds for both solid phase or soil extract based assays were well predicted by the ion competition model fitted to artificial solution data. In addition, solution based Cu(2+) EC50 of the solid phase-contact assay were never smaller than corresponding values in soil extracts suggesting no additional solid phase toxic route. By restricting the analysis to the same added species, we show that the Cu(2+) in solution represents the toxic species to this bacterium.
Assuntos
Cobre/toxicidade , Nitrosomonas europaea/efeitos dos fármacos , Poluentes do Solo/toxicidade , Cátions Bivalentes/metabolismo , Cátions Bivalentes/toxicidade , Cobre/química , Água Doce/química , Modelos Biológicos , Nitrosomonas europaea/fisiologia , Solo/química , Poluentes do Solo/químicaRESUMO
Bioreactors containing sessile bacteria (biofilms) grown on hollow fiber membranes have been used for treatment of many wastestreams. Real time operational control of bioreactor performance requires detailed knowledge of the relationship between bulk liquid water quality and physiological transport at the biofilm-liquid interface. Although large data sets exist describing membrane-aerated bioreactor effluent quality, very little real time data is available characterizing boundary layer transport under physiological conditions. A noninvasive, microsensor technique was used to quantify real time (≈1.5 s) changes in oxygen and proton flux for mature Nitrosomonas europaea and Pseudomonas aeruginosa biofilms in membrane-aerated bioreactors following exposure to environmental toxins. Stress response was characterized during exposure to toxins with known mode of action (chlorocarbonyl cyanide phenyl-hydrazone and potassium cyanide), and four environmental toxins (rotenone, 2,4-dinitrophenol, cadmium chloride, and pentachlorophenol). Exposure to sublethal concentrations of all environmental toxins caused significant increases in O(2) and/or H(+) flux (depending on the mode of action). These real time microscale signatures (i.e., fingerprints) of O(2) and H(+) flux can be coupled with bulk liquid analysis to improve our understanding of physiology in counter-diffusion biofilms found within membrane aerated bioreactors; leading to enhanced monitoring/modeling strategies for bioreactor control.
Assuntos
Poluentes Ambientais/toxicidade , Membranas Artificiais , Nitrosomonas europaea/fisiologia , Oxigênio/metabolismo , Prótons , Pseudomonas aeruginosa/fisiologia , Biofilmes/efeitos dos fármacos , Concentração de Íons de Hidrogênio/efeitos dos fármacos , Nitrosomonas europaea/efeitos dos fármacos , Cianeto de Potássio/toxicidade , Pseudomonas aeruginosa/efeitos dos fármacos , Estresse Fisiológico/efeitos dos fármacosRESUMO
The effects of CdSO(4) additions on the gene expressions of a mercury reductase, merA, an oxidative stress protein, trxA, the ammonia-monooxygenase enzyme (AMO), amoA, and the hydroxylamine oxidoreductase enzyme (HAO), hao, were examined in continuously cultured N. europaea cells. The reactor was fed 50 mM NH(4)+ and was operated for 78 days with a 6.9 days hydraulic retention time. Over this period, six successive batch additions of CdSO(4) were made with increasing maximum concentrations ranging from 1 to 60 microM Cd(2+). The expression of merA was highly correlated with the level of Cd(2+) within the reactor (Rs = 0.90) with significant up-regulation measured at non-inhibitory Cd(2+) concentrations. Cd(2+) appears to target AMO specifically at lower concentrations and caused oxidative stress at higher concentrations, as indicated by the SOURs (specific oxygen uptake rates) and the up-regulation of trxA. Since Cd(2+) inhibition is irreversible and amoA was up-regulated in response to Cd(2+) inhibition, it is hypothesized that de novo synthesis of the AMO enzyme occurred and was responsible for the observed recovery in activity. Continuously cultured N. europaea cells were more resistant to Cd(2+) inhibition than previously examined batch cultured cells due to the presence of Mg(2+) and Ca(2+) in the growth media, suggesting that Cd(2+) enters the cell through Mg(2+) and Ca(2+) import channels. The up-regulation of merA during exposure to non-inhibitory Cd(2+) levels indicates that merA is an excellent early warning signal for Cd(2+) inhibition.
Assuntos
Proteínas de Bactérias/biossíntese , Compostos de Cádmio/metabolismo , Nitrosomonas europaea/efeitos dos fármacos , Nitrosomonas europaea/fisiologia , Estresse Fisiológico , Sulfatos/metabolismo , Reatores Biológicos/microbiologia , Perfilação da Expressão Gênica , Regulação Bacteriana da Expressão Gênica , Nitrosomonas europaea/genética , Oxirredutases/biossíntese , Compostos de Amônio Quaternário/metabolismo , Tiorredoxinas/biossínteseRESUMO
Nitrosomonas europaea (ATCC 19718) is one of several nitrifying species that participate in the biological removal of nitrogen from wastewater by oxidizing ammonia to nitrite, the first step in nitrification. Because nitrification is quite sensitive to cyanide, a compound often encountered in wastewater treatment plants, we characterized the physiological and transcriptional responses of N. europaea cells to cyanide. The cells were extremely sensitive to low concentrations of cyanide, with NO-(2)production and ammonia-dependent oxygen uptake rates decreasing by 50% within 30 min of exposure to 1 microM NaCN. Whole-genome transcriptional responses of cells exposed to 1 microM NaCN were examined using Affymetrix microarrays to identify stress-induced genes. The transcript levels of 35 genes increased more than 2-fold while transcript levels of 29 genes decreased more than 20-fold. A gene cluster that included moeZ (NE2353), encoding a rhodanese homologue and thought to be involved in detoxification of cyanide, showed the highest up-regulation (7-fold). The down-regulated genes included genes encoding proteins involved in the sulfate reduction pathway, signal transduction mechanisms, carbohydrate transport, energy production, coenzyme metabolism, and amino acid transport.
Assuntos
Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Nitrosomonas europaea/efeitos dos fármacos , Nitrosomonas europaea/fisiologia , Cianeto de Sódio/farmacologia , Amônia/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , RNA Polimerases Dirigidas por DNA/genética , RNA Polimerases Dirigidas por DNA/metabolismo , Regulação para Baixo/efeitos dos fármacos , Perfilação da Expressão Gênica , Genoma Bacteriano , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Nitritos/metabolismo , Nitrosomonas europaea/genética , Análise de Sequência com Séries de Oligonucleotídeos , Oxigênio/metabolismo , Reprodutibilidade dos Testes , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fator sigma/genética , Fator sigma/metabolismo , Estresse Fisiológico/efeitos dos fármacos , Regulação para Cima/efeitos dos fármacosRESUMO
The family of ammonia and ammonium channel proteins comprises the Amt proteins, which are present in all three domains of life with the notable exception of vertebrates, and the homologous Rh proteins (Rh50 and Rh30) that have been described thus far only in eukaryotes. The existence of an RH50 gene in bacteria was first revealed by the genome sequencing of the ammonia-oxidizing bacterium Nitrosomonas europaea. Here we have used a phylogenetic approach to study the evolution of the N. europaea RH50 gene, and we show that this gene, probably as a component of an integron cassette, has been transferred to the N. europaea genome by horizontal gene transfer. In addition, by functionally characterizing the Rh50(Ne) protein and the corresponding knockout mutant, we determined that NeRh50 can mediate ammonium uptake. The RH50(Ne) gene may thus have replaced functionally the AMT gene, which is missing in the genome of N. europaea and may be regarded as a case of nonorthologous gene displacement.
Assuntos
Amônia/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/metabolismo , Nitrosomonas europaea/genética , Nitrosomonas europaea/fisiologia , Evolução Molecular , Deleção de Genes , Filogenia , Homologia de Sequência de AminoácidosRESUMO
Nitrosomonas europaea, as an ammonia-oxidizing bacterium, has a high Fe requirement and has 90 genes dedicated to Fe acquisition. Under Fe-limiting conditions (0.2 microM Fe), N. europaea was able to assimilate up to 70% of the available Fe in the medium even though it is unable to produce siderophores. Addition of exogenous siderophores to Fe-limited medium increased growth (final cell mass). Fe-limited cells had lower heme and cellular Fe contents, reduced membrane layers, and lower NH3- and NH2OH-dependent O2 consumption activities than Fe-replete cells. Fe acquisition-related proteins, such as a number of TonB-dependent Fe-siderophore receptors for ferrichrome and enterobactin and diffusion protein OmpC, were expressed to higher levels under Fe limitation, providing biochemical evidence for adaptation of N. europaea to Fe-limited conditions.
Assuntos
Adaptação Fisiológica , Ferro/metabolismo , Nitrosomonas europaea/fisiologia , Proteínas da Membrana Bacteriana Externa/biossíntese , Proteínas da Membrana Bacteriana Externa/isolamento & purificação , Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/isolamento & purificação , Biomassa , Membrana Celular/ultraestrutura , Citoplasma/química , Heme/análise , Espectrometria de Massas , Microscopia Eletrônica de Transmissão , Nitrosomonas europaea/química , Nitrosomonas europaea/crescimento & desenvolvimento , Nitrosomonas europaea/metabolismo , Consumo de Oxigênio , Porinas/biossíntese , Porinas/isolamento & purificação , Receptores de Superfície Celular/biossíntese , Receptores de Superfície Celular/isolamento & purificação , Sideróforos/metabolismoRESUMO
To remove ammonia for production of drinking water, nitrification can be performed in a bio-filter. At least 1 month is necessary to capture from the groundwater and then grow a sufficient amount of nitrifying bacteria to reach the desired removal efficiency. Improving start-up of bio-filters at low substrate concentration is therefore a major challenge. In this connection, it is important to develop appropriate models for designing, monitoring or analysing biofilm systems during start-up or following disinfection events. This study discusses the development and calibration of a nitrification PDE model which reflects the compromise between the complexity associated with the description of the full physical and biochemical mechanisms and the search for a simplified model with identifiable parameters. This model takes only the relevant phenomena (considering the full operating range) into account. The validity of the calibrated model has been evaluated through experiments under very different operational conditions, at the laboratory and under real industrial conditions, involving the full upstream chain of water treatment (iron oxidation and sand filter).
Assuntos
Amônia/isolamento & purificação , Nitrobacter/fisiologia , Nitrogênio/química , Nitrosomonas europaea/fisiologia , Purificação da Água/métodos , Abastecimento de Água , Biofilmes , Biomassa , Calibragem , Filtração , Modelos Teóricos , Nitrobacter/classificação , Nitrobacter/crescimento & desenvolvimento , Nitrogênio/análise , Nitrosomonas europaea/crescimento & desenvolvimento , Reprodutibilidade dos Testes , Fatores de TempoRESUMO
The transcriptome of Nitrosomonas europaea was analyzed with whole-genome microarrays. Growing cells were compared to cells deprived of (NH4)2SO4 and Na2CO3. Hybridization signals were detected for 76% of the genes represented on the array under either or both conditions. Transcript levels for 68% of the genes were at least twofold higher in growing cells than in deprived cells, while only 0.42% of the genes were present at more than twofold higher levels in deprived cells. Transcript levels for the remaining 7% of the genes did not change significantly with the treatments. These trends were confirmed for selected genes by Northern hybridizations and quantitative RT-PCR. Compared to heterotrophic bacteria, N. europaea downregulates a greater proportion of its genes and fewer genes appear to be associated with the adaptation to starvation.
Assuntos
Amônia/metabolismo , Proteínas de Bactérias/metabolismo , Carbonatos/metabolismo , Perfilação da Expressão Gênica , Resposta ao Choque Térmico , Nitrosomonas europaea/crescimento & desenvolvimento , Proteínas de Bactérias/genética , Regulação Bacteriana da Expressão Gênica , Nitrosomonas europaea/fisiologia , Análise de Sequência com Séries de Oligonucleotídeos , Transcrição GênicaRESUMO
Although Nitrosomonas europaea lacks measurable alpha-ketoglutarate dehydrogenase activity, the recent completion of the genome sequence revealed the presence of the genes encoding the enzyme. A knockout mutation was created in the sucA gene encoding the E1 subunit. Compared to wild-type cells, the mutant strain showed an accelerated loss of ammonia monooxygenase and hydroxylamine oxidoreductase activities upon entering stationary phase. In addition, unlike wild-type cells, the mutant strain showed a marked lag in the ability to resume growth in response to pH adjustments in late stationary phase.
Assuntos
Regulação Bacteriana da Expressão Gênica , Inativação Gênica , Complexo Cetoglutarato Desidrogenase/genética , Nitrosomonas europaea/enzimologia , Nitrosomonas europaea/crescimento & desenvolvimento , Concentração de Íons de Hidrogênio , Complexo Cetoglutarato Desidrogenase/metabolismo , Mutação , Nitrosomonas europaea/genética , Nitrosomonas europaea/fisiologia , Consumo de Oxigênio , Reação em Cadeia da PolimeraseRESUMO
As obligate chemolithotrophs, ammonia-oxidizing bacteria (AOB) grow very slowly and are known to be extremely sensitive to a wide variety of inhibitors. Since it is generally accepted that inhibition of ammonia oxidation by AOB results in a total failure of nitrogen removal, it is necessary to develop a method to detect inhibitors of ammonia oxidation in wastewater. Since ammonia oxidation accompanies oxygen consumption, ammonia oxidation can be easily evaluated by measuring oxygen consumption rate using a dissolved oxygen (DO) probe. In this study, a rapid and simple respirometric biosensor using the pure culture of Nitrosomonas europaea was developed. N. europaea was cultivated in a continuous fermentor operating at the dilution rate of 0.008 h(-1) to obtain physiologically constant cells and was immobilized onto the dialysis membrane through filtration. DO, determined by the biosensor, started to increase 30 s later after ammonia oxidation inhibitor was fed, and a new steady-state DO was obtained in 10-30 min. For this DO profile, steady-state kinetics was applied to evaluate ammonia oxidation efficiency. The concentration of a toxic compound causing 50% decrease of oxygen-consumption activity (EC50) was determined for different chemicals. The EC50 values obtained with the biosensor (0.018 mg l(-1) for allylthiourea, 0.027 mg l(-1) for thioacetamide, 1.10 mg l(-1) for phenol and 0.0 1mg l(-1) for thiourea) indicated that the developed biosensor was highly sensitive to a variety of the inhibitors. It was also shown that the biosensor is applicable for on-line real time monitoring.
Assuntos
Amônia/metabolismo , Técnicas Biossensoriais/instrumentação , Eletroquímica/instrumentação , Nitrosomonas europaea/fisiologia , Consumo de Oxigênio/fisiologia , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/farmacologia , Bioensaio/instrumentação , Bioensaio/métodos , Técnicas Biossensoriais/métodos , Células Imobilizadas/efeitos dos fármacos , Células Imobilizadas/fisiologia , Eletroquímica/métodos , Desenho de Equipamento , Análise de Falha de Equipamento , Nitrosomonas europaea/citologia , Nitrosomonas europaea/efeitos dos fármacos , Oxirredução/efeitos dos fármacos , Consumo de Oxigênio/efeitos dos fármacosRESUMO
NO, a free radical gas, is the signal for Nitrosomonas europaea cells to switch between different growth modes. At an NO concentration of more than 30 ppm, biofilm formation by N. europaea was induced. NO concentrations below 5 ppm led to a reversal of the biofilm formation, and the numbers of motile and planktonic (motile-planktonic) cells increased. In a proteomics approach, the proteins expressed by N. europaea were identified. Comparison studies of the protein patterns of motile-planktonic and attached (biofilm) cells revealed several clear differences. Eleven proteins were found to be up or down regulated. Concentrations of other compounds such as ammonium, nitrite, and oxygen as well as different temperatures and pH values had no significant effect on the growth mode of and the proteins expressed by N. europaea.