RESUMO
Multi-segmented viruses often multimerize their genomic segments to ensure efficient and stoichiometric packaging of the correct genetic cargo. In the bipartite Nodaviridae family, genome heterodimerization is also observed and conserved among different species. However, the nucleotide composition and biological function for this heterodimer remain unclear. Using Flock House virus as a model system, we developed a next-generation sequencing approach ("XL-ClickSeq") to probe heterodimer site sequences. We identified an intermolecular base-pairing site which contributed to heterodimerization in both wild-type and defective virus particles. Mutagenic disruption of this heterodimer site exhibited significant deficiencies in genome packaging and encapsidation specificity to viral genomic RNAs. Furthermore, the disruption of this intermolecular interaction directly impacts the thermostability of the mature virions. These results demonstrate that the intermolecular RNA-RNA interactions within the encapsidated genome of an RNA virus have an important role on virus particle integrity and thus may impact its transmission to a new host.IMPORTANCEFlock House virus is a member of Nodaviridae family of viruses, which provides a well-studied model virus for non-enveloped RNA virus assembly, cell entry, and replication. The Flock House virus genome consists of two separate RNA molecules, which can form a heterodimer upon heating of virus particles. Although similar RNA dimerization is utilized by other viruses (such as retroviruses) as a packaging mechanism and is conserved among Nodaviruses, the role of heterodimerization in the Nodavirus replication cycle is unclear. In this research, we identified the RNA sequences contributing to Flock House virus genome heterodimerization and discovered that such RNA-RNA interaction plays an essential role in virus packaging efficiency and particle integrity. This provides significant insight into how the interaction of packaged viral RNA may have a broader impact on the structural and functional properties of virus particles.
Assuntos
Dimerização , Genoma Viral , Nodaviridae , RNA Viral , Termodinâmica , Empacotamento do Genoma Viral , Vírion , Animais , Pareamento de Bases/genética , Genoma Viral/genética , Sequenciamento de Nucleotídeos em Larga Escala , Mutação , Nodaviridae/química , Nodaviridae/genética , Nodaviridae/crescimento & desenvolvimento , Infecções por Vírus de RNA/transmissão , Infecções por Vírus de RNA/veterinária , Infecções por Vírus de RNA/virologia , RNA Viral/química , RNA Viral/genética , RNA Viral/metabolismo , Empacotamento do Genoma Viral/genética , Vírion/química , Vírion/genética , Vírion/metabolismoRESUMO
Interspecies transmission of viruses, where a pathogen crosses species barriers and jumps from its original host into a novel species, has been receiving increasing attention. Viral covert mortality disease, caused by covert mortality nodavirus (CMNV), is an emerging disease that has recently had a substantial impact on shrimp aquaculture in Southeast Asia and Latin America. While investigating the host range of CMNV, we found that this virus is also capable of infecting populations of the farmed Japanese flounder Paralichthys olivaceus, a vertebrate host. The infected fish were being raised in aquaculture facilities that were also producing marine shrimp. Through RT-nPCR, targeting the RNA-dependent RNA polymerase (RdRp) gene of CMNV, we found that 29â% of the fish sampled were positive. The amplicons were sequenced and aligned to the RdRp gene of shrimp CMNV and were found to have 98â% identity. Histopathological examination indicated that CMNV-positive fish showed vacuolation of nervous tissue in the eye and brain, as well as extensive necrosis of cardiac muscle. In situ hybridization showed positive reactions in tissues of the eye, brain, heart, liver, spleen and kidney of infected fish. Transmission electron microscopy showed the presence of CMNV-like particles in all of the above-mentioned tissues, except for brain. The novel finding of a shrimp alphanodavirus that can also infect farmed P. olivaceus indicates that this virus is capable of naturally crossing the species barrier and infecting another vertebrate. This finding will contribute to the development of efficient strategies for disease management in aquaculture.
Assuntos
Doenças dos Peixes/virologia , Linguado/virologia , Nodaviridae/isolamento & purificação , Infecções por Vírus de RNA/veterinária , Estruturas Animais/patologia , Estruturas Animais/virologia , Animais , Aquicultura , Sudeste Asiático , Histocitoquímica , Especificidade de Hospedeiro , América Latina , Nodaviridae/classificação , Nodaviridae/genética , Nodaviridae/crescimento & desenvolvimento , Penaeidae/virologia , Infecções por Vírus de RNA/virologia , RNA Viral/genética , Análise de Sequência de DNA , Homologia de SequênciaRESUMO
The family Sarthroviridae includes a single genus, Macronovirus, which in turn includes a single species, Macrobrachium satellite virus 1. Members of this species, named extra small virus, are satellite viruses of Macrobrachium rosenbergii nodavirus, an unclassified virus related to members of the family Nodaviridae. Both viruses have isometric, spherical virions, infect giant freshwater prawns and together cause white tail disease, which is responsible for mass mortalities and severe economic losses in hatcheries and farms. Infection is caused by both vertical and horizontal transmission of virus. Aquatic insects act as a carrier to transmit the disease in prawns. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Sarthroviridae, which is available at www.ictv.global/report/sarthroviridae.
Assuntos
Nodaviridae/crescimento & desenvolvimento , Vírus de RNA/classificação , Vírus de RNA/genética , Vírus Satélites/classificação , Vírus Satélites/genética , Animais , Transmissão de Doença Infecciosa , Transmissão Vertical de Doenças Infecciosas , Insetos Vetores/virologia , Nodaviridae/ultraestrutura , Palaemonidae/virologia , Infecções por Vírus de RNA/transmissão , Infecções por Vírus de RNA/veterinária , Infecções por Vírus de RNA/virologia , Vírus de RNA/isolamento & purificação , Vírus de RNA/ultraestrutura , Vírus Satélites/isolamento & purificação , Vírus Satélites/ultraestrutura , Vírion/ultraestruturaRESUMO
The inhibitory effects of ammonium chloride (NH4Cl) and chlorpromazine hydrochloride on betanodavirus were evaluated on Sahul Indian sea bass kidney (SISK) cell line. The cytotoxicity of different concentrations of NH4Cl (0.1â¯mM, 1â¯mM, 10â¯mM, 100â¯mM and 500â¯mM) and chlorpromazine hydrochloride (1⯵M, 10⯵M, 100⯵M, 200⯵M and 500⯵M) were assessed in SISK cells using different cytotoxic assays. Among the selected concentrations, 0.1â¯mM, 1â¯mM and 10â¯mM of NH4Cl and chlorpromazine hydrochloride at the dose of 1⯵M, 10⯵M and 100⯵M were found to be non-toxic to the SISK cell line and same were chosen for the trials against nodavirus. The presence of nodavirus in the infected cells was confirmed by cytopathic effect (CPE) and RT-PCR (Reverse transcriptase PCR). NH4Cl of 1â¯mM and 10â¯mM, and chlorpromazine hydrochloride of 10⯵M and 100⯵M could successfully inhibit betanodavirus infection in SISK cells, which was confirmed by indirect ELISA and real-time PCR analysis. The result further suggested that the chlorpromazine hydrochloride drug could be more effective in inhibiting the betanodavirus with much lower dose than NH4Cl which was more effective at a higher dose. The present study thus suggested that NH4Cl and chlorpromazine hydrochloride drugs could be successfully used for controlling the nodavirus infection in aquaculture.
Assuntos
Cloreto de Amônio/farmacologia , Antivirais/farmacologia , Clorpromazina/farmacologia , Avaliação Pré-Clínica de Medicamentos , Nodaviridae/efeitos dos fármacos , Cloreto de Amônio/toxicidade , Animais , Antivirais/toxicidade , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Clorpromazina/toxicidade , Efeito Citopatogênico Viral , Ensaio de Imunoadsorção Enzimática , Peixes , Testes de Sensibilidade Microbiana , Nodaviridae/crescimento & desenvolvimento , RNA Viral/análise , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Proteínas Virais/análise , Replicação Viral/efeitos dos fármacosRESUMO
Viral covert mortality disease (VCMD) has caused serious losses to shrimp aquaculture in China in recent years and the ridgetail white prawn Exopalaemon carinicauda has been suspected to be one important factor in perpetuating the high prevalence of covert mortality nodavirus (CMNV) infections due to its perennial presence in shrimp farming ponds and water from natural habitats. Experiments were carried out to determine the possibility of vertical transmission of CMNV in E. carinicauda in this study. CMNV infection in gonads, fertilized eggs and larvae was investigated by using the methods of reverse transcription nested PCR (nRT-PCR), in situ hybrization (ISH) and transmission electron microscopy (TEM). The ovarian tissue and testis tissue of artificially infected parental E. carinicauda were proved to be CMNV-positive by nRT-PCR. Fertilized eggs were also found to be CMNV-positive by nRT-PCR whether the fertilized eggs originated from the CMNV-positive female broodstock mated with the CMNV-negative male broodstock, or they originated from the CMNV-negative female broodstock mated with the CMNV-positive males. The results of ISH indicated that the positive signals were evident in the oocytes within ovarian tissue and nauplii. By TEM analysis, CMNV virions were observed in oogonia, oocytes, spermatocytes, fertilized eggs and nauplii. The presence of CMNV in fertilized eggs and larvae indicates that CMNV can transmit vertically via sperm and oocytes in E. carinicauda, which highlights the high probability of vertical transmission of CMNV in the main species of cultured shrimp and prawns.
Assuntos
Transmissão Vertical de Doenças Infecciosas , Nodaviridae/crescimento & desenvolvimento , Palaemonidae/virologia , Animais , China , Gônadas/virologia , Hibridização In Situ , Larva/virologia , Masculino , Microscopia Eletrônica de Transmissão , Oócitos/virologia , Reação em Cadeia da Polimerase , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Espermatócitos/virologia , Zigoto/virologiaRESUMO
Piscine nodavirus (betanodavirus) is the causative agent of viral nervous necrosis (VNN) in a variety of cultured fish species, particularly marine fish. In the present study, we developed a sensitive method for cell culture isolation of the virus from seawater and applied the method to a spontaneous fish-rearing environment. The virus in seawater was concentrated by an iron-based flocculation method and subjected to isolation with E-11 cells. A real-time reverse transcriptase PCR (RT-PCR) assay was used to quantify the virus in water. After spiking into seawater was performed, a betanodavirus strain (red spotted grouper nervous necrosis virus [RGNNV] genotype) was effectively recovered in the E-11 cells at a detection limit of approximately 10(5)copies (equivalent to 10(2)50% tissue culture infective doses [TCID50])/liter seawater. In an experimental infection of juvenile sevenband grouper (Epinephelus septemfasciatus) with the virus, the virus was isolated from the drainage of a fish-rearing tank when the virus level in water was at least approximately 10(5)copies/liter. The application of this method to seven band grouper-rearing floating net pens, where VNN prevailed, resulted in the successful isolation of the virus from seawater. No differences were found in the partial sequences of the coat protein gene (RNA2) between the clinical virus isolates of dead fish and the cell-cultured virus isolates from seawater, and the viruses were identified as RGNNV. The infection experiment showed that the virus isolates from seawater were virulent to seven band grouper. These results showed direct evidence of the horizontal transmission of betanodavirus via rearing water in marine aquaculture.
Assuntos
Bass/virologia , Nodaviridae/isolamento & purificação , Água do Mar/virologia , Cultura de Vírus , Animais , Doenças dos Peixes/virologia , Nodaviridae/crescimento & desenvolvimento , Infecções por Vírus de RNA/virologia , RNA Viral/genética , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNARESUMO
We previously reported that betanodavirus reassortant strains [redspotted grouper nervous necrosis virus/striped jack nervous necrosis virus (SJNNV)] isolated from Senegalese sole (Solea senegalensis) exhibited a modified SJNNV capsid amino acid sequence, with changes at aa 247 and 270. In the current study, we investigated the possible role of both residues as putative virulence determinants. Three recombinant viruses harbouring site-specific mutations in the capsid protein sequence, rSs160.03247 (S247A), rSs160.03270 (S270N) and rSs160.03247+270 (S247A/S270N), were generated using a reverse genetics system. These recombinant viruses were studied in cell culture and in vivo in the natural fish host. The three mutant viruses were shown to be infectious and able to replicate in E-11 cells, reaching final titres similar to the WT virus, although with a somewhat slower kinetics of replication. When the effect of the amino acid substitutions on virus pathogenicity was evaluated in Senegalese sole, typical clinical signs of betanodavirus infection were observed in all groups. However, fish mortality induced by all three mutant viruses was clearly affected. Roughly 40 % of the fish survived in these three groups in contrast with the WT virus which killed 100 % of the fish. These data demonstrated that aa 247 and 270 play a major role in betanodavirus virulence although when both mutated aa 247 and 270 are present, corresponding recombinant virus was not further attenuated.
Assuntos
Proteínas do Capsídeo/metabolismo , Doenças dos Peixes/patologia , Doenças dos Peixes/virologia , Nodaviridae/fisiologia , Infecções por Vírus de RNA/veterinária , Vírus Reordenados/fisiologia , Fatores de Virulência/metabolismo , Substituição de Aminoácidos , Animais , Proteínas do Capsídeo/genética , Linhagem Celular , Linguados/virologia , Mutação de Sentido Incorreto , Nodaviridae/genética , Nodaviridae/crescimento & desenvolvimento , Nodaviridae/patogenicidade , Mutação Puntual , Infecções por Vírus de RNA/patologia , Infecções por Vírus de RNA/virologia , Vírus Reordenados/genética , Vírus Reordenados/crescimento & desenvolvimento , Vírus Reordenados/patogenicidade , Genética Reversa , Análise de Sobrevida , Carga Viral , Virulência , Fatores de Virulência/genética , Replicação ViralRESUMO
UNLABELLED: Drosophila C virus (DCV) is a positive-sense RNA virus belonging to the Dicistroviridae family. This natural pathogen of the model organism Drosophila melanogaster is commonly used to investigate antiviral host defense in flies, which involves both RNA interference and inducible responses. Although lethality is used routinely as a readout for the efficiency of the antiviral immune response in these studies, virus-induced pathologies in flies still are poorly understood. Here, we characterize the pathogenesis associated with systemic DCV infection. Comparison of the transcriptome of flies infected with DCV or two other positive-sense RNA viruses, Flock House virus and Sindbis virus, reveals that DCV infection, unlike those of the other two viruses, represses the expression of a large number of genes. Several of these genes are expressed specifically in the midgut and also are repressed by starvation. We show that systemic DCV infection triggers a nutritional stress in Drosophila which results from intestinal obstruction with the accumulation of peritrophic matrix at the entry of the midgut and the accumulation of the food ingested in the crop, a blind muscular food storage organ. The related virus cricket paralysis virus (CrPV), which efficiently grows in Drosophila, does not trigger this pathology. We show that DCV, but not CrPV, infects the smooth muscles surrounding the crop, causing extensive cytopathology and strongly reducing the rate of contractions. We conclude that the pathogenesis associated with systemic DCV infection results from the tropism of the virus for an important organ within the foregut of dipteran insects, the crop. IMPORTANCE: DCV is one of the few identified natural viral pathogens affecting the model organism Drosophila melanogaster. As such, it is an important virus for the deciphering of host-virus interactions in insects. We characterize here the pathogenesis associated with DCV infection in flies and show that it results from the tropism of the virus for an essential but poorly characterized organ in the digestive tract, the crop. Our results may have relevance for other members of the Dicistroviridae, some of which are pathogenic to beneficial or pest insect species.
Assuntos
Dicistroviridae/crescimento & desenvolvimento , Drosophila melanogaster/virologia , Obstrução Intestinal/virologia , Animais , Dicistroviridae/fisiologia , Feminino , Trato Gastrointestinal/patologia , Trato Gastrointestinal/fisiopatologia , Trato Gastrointestinal/virologia , Perfilação da Expressão Gênica , Músculo Liso/virologia , Nodaviridae/crescimento & desenvolvimento , Sindbis virus/crescimento & desenvolvimento , Tropismo ViralRESUMO
We generalize the concept of allostery from the traditional non-active-site control of enzymes to virus maturation. Virtually, all animal viruses transition from a procapsid noninfectious state to a mature infectious state. The procapsid contains an encoded chemical program that is executed following an environmental cue. We developed an exceptionally accessible virus system for the study of the activators of maturation and the downstream consequences that result in particle stability and infectivity. Nudaurelia capensis omega virus (NωV) is a T=4 icosahedral virus that undergoes a dramatic maturation in which the 490-Å spherical procapsid condenses to a 400-Å icosahedral-shaped capsid with associated specific auto-proteolysis and stabilization. Employing X-ray crystallography, time-resolved electron cryo-microscopy and hydrogen/deuterium exchange as well as biochemistry, it was possible to define the mechanisms of allosteric communication among the four quasi-equivalent subunits in the icosahedral asymmetric unit. These gene products undergo proteolysis at different rates, dependent on quaternary structure environment, while particle stability is conferred globally following only a few local subunit transitions. We show that there is a close similarity between the concepts of tensegrity (associated with geodesic domes and mechanical engineering) and allostery (associated with biochemical control mechanisms).
Assuntos
Montagem de Vírus/fisiologia , Regulação Alostérica , Animais , Homeostase , Mariposas/virologia , Nodaviridae/crescimento & desenvolvimento , Nodaviridae/fisiologiaRESUMO
An optimized culture method for detection of infection of fish with the Red spotted grouper nervous necrosis virus (RGNNV) genotype of betanodavirus in striped snakehead (SSN-1, Channa striatus) cells is described. Inoculation of fish tissue homogenates at the same time or within 4 hr of seeding the SSN-1 cells was as sensitive as the method recommended by the World Organization for Animal Health, where homogenates were adsorbed onto an established cell monolayer. Such modification halved the time required and the costs of consumables, and reduced the potential for error when processing large numbers of samples. Positive culture results were obtained from 88.3% of 392 fish tissue homogenates in which RGNNV was detected using a quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay; 99.7% of 943 tissue homogenates, which were qRT-PCR negative, were cell culture negative. Cytopathic effect (CPE) was characterized by large intracytoplasmic vacuoles in 0.1-60% of cells. Detachment of affected cells from the culture surface resulting in progressive disruption of the monolayer occurred in 46.4% of primary cultures and 96.0% of subcultures of positive samples. Identification of CPE that did not disrupt the cell monolayer increased estimates of the 50% tissue culture infective dose (TCID(50)) by 1.07-2.79 logs (95% confidence interval). The predicted mean TCID(50)/ml was 3.3 logs higher when cells were inoculated less than 36 hr after subculture at less than 80% confluence compared to cells inoculated at greater than 80% confluence and more than 36 hr after subculture (P < 0.05).
Assuntos
Doenças dos Peixes/virologia , Nodaviridae/crescimento & desenvolvimento , Perciformes/virologia , Infecções por Vírus de RNA/veterinária , Animais , Técnicas de Cultura de Células/veterinária , Células Cultivadas/virologia , Nodaviridae/isolamento & purificação , Infecções por Vírus de RNA/virologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , TemperaturaRESUMO
Little is known about the molecular determinants causing and sustaining viral persistent infections at the cellular level. We found that Drosophila cells persistently infected (PI) with Flock House virus (FHV) invariably harbor defective viral RNAs, which are replicated by the FHV RNA-dependent RNA polymerase. Some defective RNAs encoded a functional B2 protein, the FHV suppressor of RNA interference, which might contribute to maintenance of virus persistence. Viral small interfering RNAs (vsiRNAs) of both polarities were detected in PI cells and primarily mapped to regions of the viral genome that were preserved in the isolated defective RNAs. This indicated that defective RNAs could represent major sources of vsiRNAs. Immunofluorescence analysis revealed that mitochondria and viral proteins are differentially distributed in PI cells and lytically infected cells, which may partly explain the reduction in infectious viral progeny. Our results provide a basis for further investigations of the molecular mechanisms underlying persistent infections.
Assuntos
Vírus Defeituosos/genética , Drosophila/virologia , Nodaviridae/crescimento & desenvolvimento , Animais , Linhagem Celular , Dados de Sequência Molecular , Nodaviridae/genética , RNA Viral/biossíntese , RNA Viral/genética , Análise de Sequência de DNARESUMO
A new cell line, Indian Catfish Fin, derived from the fin tissue of Indian walking catfish, Clarias batrachus, was established and characterized. The cell line grew well in Leibovitz's L-15 medium supplemented with 15% foetal bovine serum (FBS) and has been subcultured more than 110 times since its initiation in 2007. The cells were able to grow at a range of temperature from 28 to 37 °C with optimal growth at 28 °C. The cell line predominantly consists of fibroblast-like cells. The growth rate of fin cells increased as the FBS concentration increased from 2% to 20% at 28 °C with optimum growth at a concentration of 15% or 20% and poor growth at a concentration of 5%. The cells were found to be susceptible to fish nodavirus and IPNV-ab and infection was confirmed by cytopathic effect and reverse transcriptase-polymerase chain reaction. PCR amplification of mitochondrial 12S rRNA using primers specific to C. batrachus confirmed the catfish origin of the cell line. The cell line was characterized further by immunocytochemistry, transfection efficiency with pEGFP-N1 and cell cycle analysis by fluorescent-activated cell sorting.
Assuntos
Nadadeiras de Animais/citologia , Peixes-Gato , Linhagem Celular , Animais , Técnicas de Cultura de Células/métodos , Técnicas de Cultura de Células/veterinária , Processos de Crescimento Celular/fisiologia , Linhagem Celular/fisiologia , Linhagem Celular/virologia , Criopreservação , Peixes , Vírus da Necrose Pancreática Infecciosa/crescimento & desenvolvimento , Nodaviridae/crescimento & desenvolvimento , Temperatura , Replicação ViralRESUMO
Viruses are intracellular pathogens that are dependent on viral and host factors for multiplication. Model hosts, such as yeast, can be very valuable in identifying host factors involved in viral replication. Yeast is also useful for studies on functional interactions of host factors with viral proteins and/or virus nucleic acids. The advantages of using yeast include the availability of a single gene-deletion library and the essential gene library (yTHC); the controllable small- or large-scale expression of viral proteins and nucleic acids; and the rapid growth of yeast strains. Procedures that facilitate high-throughput analysis of host factors and plant and animal RNA virus replication in yeast, with a plant virus (tombusvirus; TBSV) and an animal virus (nodavirus; FHV) as examples, are described.
Assuntos
Nodaviridae/fisiologia , Saccharomyces cerevisiae/virologia , Tombusvirus/fisiologia , Virologia/métodos , Replicação Viral , Nodaviridae/crescimento & desenvolvimento , Tombusvirus/crescimento & desenvolvimentoRESUMO
Betanodaviruses, the causative agents of viral nervous necrosis in marine fish, have bipartite positive-sense RNA genomes. The larger genomic segment, RNA1 (~3.1 kb), encodes an RNA-dependent RNA polymerase (protein A), and the smaller genomic segment RNA2 (~1.4 kb) codes for the coat protein. These viruses can be classified into four genotypes, designated striped jack nervous necrosis virus (SJNNV), redspotted grouper nervous necrosis virus (RGNNV), tiger puffer nervous necrosis virus (TPNNV), and barfin flounder nervous necrosis virus (BFNNV), based on similarities in their partial RNA2 sequences. The optimal temperatures for the growth of these viruses are 20-25°C (SJNNV), 25-30°C (RGNNV), 20°C (TPNNV), and 15-20°C (BFNNV). However, little is known about the mechanisms underlying the temperature sensitivity of these viruses. We first constructed two reassortants between SJNNV and RGNNV to test their temperature sensitivity. The levels of viral growth and RNA replication of these reassortants and parental viruses in cultured fish cells were similar at 25°C. However, the levels of all of the viruses but RGNNV were markedly reduced at 30°C. These results indicate that both RNA1 and RNA2 control the temperature sensitivity of betanodaviruses by modulating RNA replication or earlier viral growth processes. We then constructed ten mutated RGNNVs, the RNA1 segments of which were chimeric between SJNNV and RGNNV, and showed that only chimeric viruses bearing the RGNNV RNA1 region, encoding amino acid residues 1-445, grew similarly to the parental RGNNV at 30°C. This portion of protein A is known to serve as a mitochondrial-targeting signal rather than functioning as an enzymatic domain.
Assuntos
Temperatura Alta , Nodaviridae/crescimento & desenvolvimento , Nodaviridae/genética , RNA Viral/genética , Replicação Viral/efeitos da radiação , Animais , Linhagem Celular , Peixes , Viabilidade Microbiana/efeitos da radiação , Nodaviridae/efeitos da radiação , Vírus Reordenados , Recombinação GenéticaRESUMO
Wuhan Nodavirus (WhNV) is the first reported nodavirus isolated from insect in China. The viral genome consists of two positive-strand RNA, RNA1 and RNA2. RNA1 is 3149 nucleotides in length, and contains three putative Open Reading Frames (ORFs) which encode proteins A, B1 and B2, respectively. In contrast, only one putative ORF encoding protein alpha was identified within 1562-nt-long RNA2 species. Here, we report the newly characterized molecular properties of WhNV subgenomic RNA3 and its encoded protein B2. We have successfully multiplied WhNV in the natural host Pieris rapae larvae under laboratory conditions. WhNV replication in the host cells resulted in the expression of viral proteins, ProA, B2 and Proalpha, with the absence of B1 production. Northern blot hybridization assay revealed the existence of subgenomic RNA3 which is 5' capped and 3' co-terminal with RNA1. The subgenomic RNA3 is 370 nucleotides in length and contains only one ORF (B2) with the first AUG as the authentic initiation codon. In addition, we found that nonstructural protein B2 of WhNV is an efficient RNA interference (RNAi) suppressor in a cultured drosophila cell line. The amino-terminal region (aa 1-20) of B2 is essential for this RNAi inhibition activity.
Assuntos
Inativação Gênica , Nodaviridae/genética , RNA Interferente Pequeno/genética , RNA Viral/genética , Proteínas Virais/biossíntese , Animais , Northern Blotting , China , Larva/virologia , Lepidópteros/virologia , Nodaviridae/crescimento & desenvolvimento , Nodaviridae/isolamento & purificação , Proteínas Virais/genéticaRESUMO
Betanodavirus, a bipartite RNA virus of fishes and a member of Nodaviridae family, targets nervous tissues and is the causative agent of viral nervous necrosis in marine farmed fish. Betanodavirus is thought to be propagated only in fish cells because betanodavirus has only been isolated in fish and it is not well propagated in mammalian culture cells. However, the host specificity of betanodavirus has not yet been well analyzed. To analyze the host specificity of betanodavirus, various mammalian cells were screened for their permissiveness to betanodavirus. As a result, redspotted grouper nervous necrosis virus can be propagated efficiently in the murine astrocytoma cell line, DBT. The level of viral production in DBT was 10-fold-higher than in the fish cell line, E-11. This result is the first to demonstrate the efficient propagation of betanodavirus in mammalian cells and may help to elucidate the mechanism of the host specificity of betanodavirus.
Assuntos
Astrocitoma/virologia , Nodaviridae/crescimento & desenvolvimento , Virologia/métodos , Animais , Técnicas de Cultura de Células , Linhagem Celular , CamundongosRESUMO
Fish nodaviruses are causative agents of viral nervous necrosis causing high mortality in cultured marine fishes around the world. The first successful isolation of fish nodavirus was made with SSN-1 cells, which are persistently infected with snakehead retrovirus (SnRV). In the present study, a BF-2 cell line persistently infected with SnRV (PI-BF-2) was established to evaluate the influence of SnRV on the production of fish nodavirus. The PI-BF-2 cells were slightly more slender than BF-2 cells, but no difference was observed in propagation rate between both cell lines. No difference was observed in production of SnRV between PI-BF-2 and SSN-1 cell lines. Although both PI-BF-2 and BF-2 cell lines showed no cytopathic effect (CPE) after inoculation of striped jack nervous necrosis virus (SJNNV) and redspotted grouper nervous necrosis virus (RGNNV), these fish nodaviruses could be amplified in BF-2 cells, and moreover, production of fish nodaviruses in the PI-BF-2 cell line was more than 40 times higher than in BF-2 cells. Thus, it was concluded that BF-2 cell permissiveness to fish nodaviruses was enhanced by persistent infection with SnRV. Furthermore, homologous cDNA to genomic RNA of SJNNV was detected from both PI-BF-2 and SSN-1 cell lines persistently infected with SnRV. The amount of nodavirus cDNA in SJNNV-inoculated PI-BF-2 cells was clearly lower than that in SJNNV-inoculated SSN-1 cells.
Assuntos
Epsilonretrovirus/fisiologia , Nodaviridae/crescimento & desenvolvimento , Perciformes/virologia , Animais , Linhagem Celular , Epsilonretrovirus/genética , Epsilonretrovirus/crescimento & desenvolvimento , Genes pol/genética , Nodaviridae/genética , Fatores de TempoRESUMO
This study evaluated the possible use of the fish SSN-1 cell line to investigate the development of Macrobrachium rosenbergii nodavirus (MrNV). Cells were incubated with viral particles and cytopathic effects were observed. De novo synthesis of viral capsid proteins was shown by immuno-fluorescence labelling and a sandwich ELISA test. Viral genomic replication was demonstrated by RT-PCR using primers specific to RNA-1 as well as by quantitative RT-PCR (RT-qPCR). Using electron microscopy, only a few empty particles were observed and attempts to isolate complete infectious particles or to re-infect healthy cells (second passage) were unsuccessful. As complete viral particles were rarely observed, it appeared that defaults in MrNV virogenesis might arise resulting in the formation of scarce and non-infectious particles. SSN-1 cells were found to be partially permissive to MrNV infection that induced cell lysis, but key elements for viral infection were lacking such as regulatory factors for gene replication or post-translational modifications.
Assuntos
Nodaviridae/patogenicidade , Palaemonidae/virologia , Perciformes/virologia , Animais , Anticorpos Antivirais/metabolismo , Antígenos Virais/análise , Antígenos Virais/metabolismo , Linhagem Celular , Efeito Citopatogênico Viral , Ensaio de Imunoadsorção Enzimática , Genoma Viral/fisiologia , Microscopia de Fluorescência , Nodaviridae/crescimento & desenvolvimento , Nodaviridae/isolamento & purificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de Tempo , Proteínas Virais/análiseRESUMO
Flock House Virus (family Nodaviridae, genus Alphanodavirus, FHV) was originally isolated from grass grubs Costelytra zealandica (White) (Coleoptera: Scarabaeidae) in New Zealand and belongs to a family of divided genome, plus-sense RNA insect viruses. FHV replicates in insects, a nematode, plants, and yeast. We previously reported replication of FHV in four genera of mosquitoes and expression of green fluorescent protein in Aedes aegypti (L.) produced by an FHV-based vector. We report here that FHV multiplies vigorously in vivo in the malaria vectors Anopheles gambiae Giles and An. stephensi Liston and in vitro in a cell line derived from An. gambiae. In addition, FHV multiplies extensively in two other medically important insects, the tsetse fly, Glossina morsitans morsitans Westwood, and the reduviid bug Rhodnius prolixus Stal, extending its host range to four orders of insects (Coleoptera, Lepidoptera, Diptera, and Hemiptera). The virus disseminates in all the major tissues of the insects studied. Anopheles and Glossina show mortality when FHV is injected at a dose above 10(4) plaque-forming units (pfu) or the virus accumulates to titer above 10(8) pfu. A lower dose (10(3) pfu) promotes more extensive virus multiplication and reduces mortality to < 10%. No adverse effects are observed in Ae. aegypti, Culex pipiens pipiens L., and Armigeres subalbatus (Coquillett), when injected with a dose of up to 10(7) pfu. Mosquitoes orally fed with FHV exhibited slower virus growth rate with lower mortality. Our results indicate that FHV has uniquely broad insect host range and that the virus can be used to study virus host interactions in a variety of medically important insects.
Assuntos
Insetos Vetores/virologia , Insetos/virologia , Nodaviridae/crescimento & desenvolvimento , Replicação Viral/fisiologia , Animais , Linhagem Celular , Especificidade da Espécie , Análise de Sobrevida , Fatores de TempoRESUMO
During infection by diverse viral families, RNA replication occurs on the surface of virally induced cytoplasmic membranes of cellular origin. How this process is regulated, and which cellular factors are required, has been unclear. Moreover, the host-pathogen interactions that facilitate the formation of this new compartment might represent critical determinants of viral pathogenesis, and their elucidation may lead to novel insights into the coordination of vesicular trafficking events during infection. Here we show that in Drosophila cells, Drosophila C virus remodels the Golgi apparatus and forms a novel vesicular compartment, on the surface of which viral RNA replication takes place. Using genome-wide RNA interference screening, we found that this step in the viral lifecycle requires at least two host encoded pathways: the coat protein complex I (COPI) coatamer and fatty acid biosynthesis. Our results integrate, clarify, and extend numerous observations concerning the cell biology of viral replication, allowing us to conclude that the coupling of new cellular membrane formation with the budding of these vesicles from the Golgi apparatus allows for the regulated generation of this new virogenic organelle, which is essential for viral replication. Additionally, because these pathways are also limiting in flies and in human cells infected with the related RNA virus poliovirus, they may represent novel targets for antiviral therapies.