RESUMO
PURPOSE: Head rotation is thought to have an effect on obstructive sleep apnea (OSA) severity. However, keeping the head rotated fully during sleep is difficult to maintain, and the effect of head rotation is not the same in all OSA patients. Thus, this study aimed to identify whether less head rotation has an effect on airway patency and determine the responder characteristics to the head rotation maneuver (HRM). METHODS: We recruited 221 patients who underwent overnight polysomnography and drug-induced sleep endoscopy (DISE) in a tertiary hospital from June 2019 to July 2020. Airway patency and the site of airway collapse were determined in the supine position with the head at 0, 30, and 60 degrees of rotation (HRM0°, HRM30°, and HRM60°, respectively) during DISE. The site of collapse was determined using the VOTE classification system: the velum (palate), oropharyngeal lateral walls, tongue base, and epiglottis. Each structure was labeled as 0, 1, or 2 (patent, partially obstructed, and completely obstructed, respectively). Airway response to the HRM30° and 60° and the clinical characteristics associated with airway opening were analyzed. RESULTS: The study population had a median age of 52 (25-61) years, a body mass index of 26.7(24.6-29.4) kg/m2, and the apnea-hypopnea index (AHI) of 28.2(13.7-71.9) events/h. HRM influenced airway patency positively not only with HRM60° (p<0.001) but also following limited rotation (HRM30°, p<0.001). Patients with tongue base (40.0% with HRM 60°) and epiglottic (52.6% with HRM 60°) collapse responded particularly well to HRM. Multivariate analysis revealed that lower AHI (p<0.001) and an absence of oropharyngeal lateral walls collapse (p = 0.011) were significant predictors of responders to HRM. CONCLUSION: Head rotation improved airway obstruction in OSA patients, even with a small degree of rotation, and should be further explored as a potential form of therapy in appropriately selected patients.
Assuntos
Obstrução das Vias Respiratórias/terapia , Orofaringe/patologia , Choque , Apneia Obstrutiva do Sono/terapia , Adulto , Endoscopia , Epiglote/fisiologia , Cabeça/fisiologia , Humanos , Pessoa de Meia-Idade , Análise Multivariada , Orofaringe/fisiopatologia , Palato/fisiologia , Polissonografia , Rotação , Sono , Centros de Atenção Terciária , Língua/fisiologiaRESUMO
Lateral tongue bracing is a lingual posture in which the sides of the tongue are held against the palate and upper molars, and has been observed cross-linguistically. However, it is unknown whether lateral bracing makes adjustments to external perturbation like other body postures. The present study aims to test the robustness of lateral tongue bracing with three experiments. The first baseline experiment was an analysis of an electropalatogram database and the results showed lateral bracing being continuously maintained. The second experiment applied an external perturbation during speech production. A bite block was held between participants' teeth while intra-oral video was used to record contact between the sides of the tongue and upper molars during speech. The results indicated that lateral bracing was maintained most of the time during speech. The third experiment included simulations investigating the activation of tongue muscles relevant to lateral bracing at different degrees of jaw opening. The results show that bracing requires higher activation of bracing agonists and lower activation of bracing antagonists as jaw opening increases. Our results suggest that lateral tongue bracing is actively maintained and robust under external perturbation and further indicate it serves as an essential lingual posture during speech production.
Assuntos
Palato , Fala , Humanos , Fala/fisiologia , Medida da Produção da Fala , Palato/fisiologia , Postura , Língua/fisiologiaRESUMO
Successful adaptation to wearing dentures with palatal coverage may be associated with cortical activity changes related to tongue motor control. The purpose was to investigate the brain activity changes during tongue movement in response to a new oral environment. Twenty-eight fully dentate subjects (mean age: 28.6-years-old) who had no experience with removable dentures wore experimental palatal plates for 7 days. We measured tongue motor dexterity, difficulty with tongue movement, and brain activity using functional magnetic resonance imaging during tongue movement at pre-insertion (Day 0), as well as immediately (Day 1), 3 days (Day 3), and 7 days (Day 7) post-insertion. Difficulty with tongue movement was significantly higher on Day 1 than on Days 0, 3, and 7. In the subtraction analysis of brain activity across each day, activations in the angular gyrus and right precuneus on Day 1 were significantly higher than on Day 7. Tongue motor impairment induced activation of the angular gyrus, which was associated with monitoring of the tongue's spatial information, as well as the activation of the precuneus, which was associated with constructing the tongue motor imagery. As the tongue regained the smoothness in its motor functions, the activation of the angular gyrus and precuneus decreased.
Assuntos
Adaptação Fisiológica , Encéfalo/fisiologia , Imageamento por Ressonância Magnética , Movimento/fisiologia , Palato/fisiologia , Língua/fisiologia , Adulto , Mapeamento Encefálico , Feminino , Humanos , Masculino , Atividade Motora/fisiologiaRESUMO
Proper superimposition of intraoral scan generated 3D models enables detailed assessment of soft and hard tissue surface changes. This requires accurate 3D models and stable structures as superimposition references. In the maxilla, different reference areas have been proposed, mostly located at the palatal region. In this in vivo study we evaluated the precision of two intraoral scanners (TRIOS 3, 3Shape and CS 3600, Carestream) at the maxilla, focusing on the palate itself and also on its spatial relation to the dentition, following palatal superimposition. Precision was tested through the superimposition of repeated scans on the palate and the dental arch. Overall, the median precision of both scanners was high (< 0.1 mm). Scanner precision was comparable when the palatal area was tested individually. However, TRIOS 3 showed higher precision regarding the assessment of the dental arch, following superimposition of repeated models on the palate (median difference: approximately 40 µm). In few cases, local areas of higher imprecision were present for both scanners, exceeding 0.3 mm. Thus, scanner precision seems to be high in small, but slightly reduced considering larger areas, with differences between scanners. However, the effect on individual tooth position relative to the palate was for both scanners limited.
Assuntos
Dentição , Diagnóstico por Imagem/instrumentação , Maxila/fisiologia , Palato/fisiologia , Adulto , Algoritmos , Desenho Assistido por Computador , Feminino , Humanos , Imageamento Tridimensional , Masculino , Pessoa de Meia-Idade , Modelos Anatômicos , Polivinil/química , Reprodutibilidade dos Testes , Siloxanas/química , Software , Dente/fisiologiaRESUMO
The median palatal suture serves as a growth center for the maxilla; inadequate growth at this site causes malocclusion and dental crowding. However, the pattern formation mechanism of palatal sutures is poorly understood compared with that of calvarial sutures such as the sagittal suture. In the present study, therefore, we compared the morphological characteristics of sagittal and palatal sutures in human bone specimens. We found that palatal suture width was narrower than sagittal suture width, and the interdigitation amplitude of the palatal suture was lower than that of the sagittal suture. These tendencies were also observed in the neonatal stage. However, such differences were not observed in other animals such as chimpanzees and mice. We also used a mathematical model to reproduce the differences between palatal and sagittal sutures. After an extensive parameter search, we found two conditions that could generate the difference in interdigitation amplitude and suture width: bone differentiation threshold [Formula: see text] and growth speed c. We discuss possible biological interpretations of the observed pattern difference and its cause.
Assuntos
Suturas Cranianas , Modelos Biológicos , Modelos Teóricos , Palato , Animais , Suturas Cranianas/anatomia & histologia , Suturas Cranianas/fisiologia , Humanos , Camundongos , Palato/anatomia & histologia , Palato/fisiologia , Pan troglodytesRESUMO
To evaluate the efficiency of photobiomodulation therapy (PBMT) in the midpalatal suture (MPS) and pain sensation in patients undergoing rapid palatal expansion (RPE). Thirty-four individuals with the diagnosis of skeletal maxillary hypoplasia were divided in two groups: laser (n = 18) and control (n = 16). Treatment plan consisted of the use of the Hyrax expander in all patients. Subjects in the laser group were irradiated with diode laser (980 nm, 0.3 W) in six spots bilaterally distributed along the MPS for 10 s during the active phase of treatment and after overcorrection (passive phase of RPE). Control group received sham irradiations with the laser in standby mode to characterize the placebo effect. Digital occlusal radiographs were performed at different time-points for bone formation evaluation in both groups. The effects of laser irradiation on pain were assessed by the visual analog scale (Wong-Baker Faces Pain Scale). Bone formation between groups was not significantly different (p = 0.2273). At 3 months, bone formation was not yet complete in both groups. Pain sensation was similar between groups (p = 0.3940). However, pain was significantly higher for the first 7 days of treatment compared with the 14th day. PBMT did not accelerate bone regeneration in the MPS and pain sensation was similar.
Assuntos
Terapia com Luz de Baixa Intensidade , Osteogênese/efeitos da radiação , Técnica de Expansão Palatina , Palato/fisiologia , Palato/efeitos da radiação , Suturas , Regeneração Óssea/efeitos da radiação , Humanos , MasculinoRESUMO
Palate fullness and mouthfeel of beer are key attributes of sensory beer quality. Non-volatile substances and molar mass fractions influence sensory perceptions of palate fullness and mouthfeel. However, systematic correlations between sensory attributes and native beer compounds have not been evaluated within the concentration range found in lager beer. This article reports a chemometric analysis of 41 lager beers by evaluating analytical data of beer compositions, palate fullness, and mouthfeel descriptors. AF4-MALS-dRI indicated high variability in the macromolecular compositions of classical lager beers. Screened beers were clustered into groups differing significantly in palate fullness intensity and macromolecular distribution. Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and ß-glucan (p < 0.01). Thus, a model was built using partial least square regression (PLS) analysis to predict the palate fullness intensity in beers (R2C = 0.7993). This model can be used as a guideline by brewers to control palate fullness and mouthfeel.
Assuntos
Cerveja/análise , Informática , Palato/fisiologia , Paladar , Humanos , Análise dos Mínimos Quadrados , Substâncias Macromoleculares/análise , Peso Molecular , Viscosidade , beta-Glucanas/análiseRESUMO
BACKGROUND: Despite the strides made in understanding the complex network of key regulatory genes and cellular processes that drive palate morphogenesis, patients suffering from these conditions face treatment options that are limited to complex surgeries and multidisciplinary care throughout life. Hence, a better understanding of how molecular interactions drive palatal growth and fusion is critical for the development of treatment and preventive strategies for cleft palates in humans. Our previous work demonstrated that Pax9-dependent Wnt signaling is critical for the growth and fusion of palatal shelves. We showed that controlled intravenous delivery of small molecule Wnt agonists specifically blocks the action of Dkks (inhibitors of Wnt signaling) and corrects secondary palatal clefts in Pax9-/- mice. While these data underscore the importance of the functional upstream relationship of Pax9 to the Wnt pathway, not much is known about how the genetic nature of Pax9's interactions in vivo and how it modulates the actions of these downstream effectors during palate formation. RESULTS: Here, we show that the genetic reduction of Dkk1 during palatogenesis corrected secondary palatal clefts in Pax9-/- mice with restoration of Wnt signaling activities. In contrast, genetically induced overexpression of Dkk1 mice phenocopied the defects in tooth and palate development visible in Pax9-/- strains. Results of ChIP-qPCR assays showed that Pax9 can bind to regions near the transcription start sites of Dkk1 and Dkk2 as well as the intergenic region of Wnt9b and Wnt3 ligands that are downregulated in Pax9-/- palates. CONCLUSIONS: Taken together, these data suggest that the molecular mechanisms underlying Pax9's role in modulating Wnt signaling activity likely involve the inhibition of Dkk expression and the control of Wnt ligands during palatogenesis.
Assuntos
Fator de Transcrição PAX9/genética , Fator de Transcrição PAX9/fisiologia , Palato/embriologia , Proteínas Wnt/genética , Animais , Fissura Palatina/genética , Modelos Animais de Doenças , Feminino , Genótipo , Ligantes , Masculino , Mesoderma , Camundongos , Mutação , Palato/fisiologia , Fenótipo , Transdução de Sinais , Proteínas Wnt/metabolismo , Proteína Wnt3/genéticaRESUMO
Auditory speech perception enables listeners to access phonological categories from speech sounds. During speech production and speech motor learning, speakers' experience matched auditory and somatosensory input. Accordingly, access to phonetic units might also be provided by somatosensory information. The present study assessed whether humans can identify vowels using somatosensory feedback, without auditory feedback. A tongue-positioning task was used in which participants were required to achieve different tongue postures within the /e, ε, a/ articulatory range, in a procedure that was totally nonspeech like, involving distorted visual feedback of tongue shape. Tongue postures were measured using electromagnetic articulography. At the end of each tongue-positioning trial, subjects were required to whisper the corresponding vocal tract configuration with masked auditory feedback and to identify the vowel associated with the reached tongue posture. Masked auditory feedback ensured that vowel categorization was based on somatosensory feedback rather than auditory feedback. A separate group of subjects was required to auditorily classify the whispered sounds. In addition, we modeled the link between vowel categories and tongue postures in normal speech production with a Bayesian classifier based on the tongue postures recorded from the same speakers for several repetitions of the /e, ε, a/ vowels during a separate speech production task. Overall, our results indicate that vowel categorization is possible with somatosensory feedback alone, with an accuracy that is similar to the accuracy of the auditory perception of whispered sounds, and in congruence with normal speech articulation, as accounted for by the Bayesian classifier.
Assuntos
Retroalimentação Fisiológica , Fonética , Sensação/fisiologia , Percepção da Fala/fisiologia , Língua/fisiologia , Adulto , Feminino , Humanos , Masculino , Palato/fisiologia , Medida da Produção da Fala , Adulto JovemRESUMO
The hold phase of the stop consonants is crucial for a successful production of the release and the acoustic burst. Concurrently, it is also associated with weak acoustic energy and minimal movement, so that conventional acoustic and kinematic approaches are not relevant to investigate motor control. This paper presents an innovative experimental method to study speech motor control during this phase, based on meticulous measurement of the time variation of the mechanical pressure exerted by the tongue against the palate and also characterizing tongue-palate interaction. The concept is based on using miniature transducers with enhanced response characteristics inserted in different locations of the complete denture of edentulous subjects without perturbing the articulation. The study was done with a French-speaking adult whose maxillary denture was duplicated and mounted with six strain gauge transducers. The experiment was done with denti-alveolar and velar stop consonants with two vowel contexts. The results illustrate the potential of such device to analyse speech motor control when contact constrains tongue movements.
Assuntos
Idioma , Palato/fisiologia , Pressão , Língua/fisiologia , Fenômenos Biomecânicos , Dentaduras , França , Humanos , Masculino , Pessoa de Meia-Idade , TransdutoresRESUMO
The extinct nonavian dinosaur Tyrannosaurus rex, considered one of the hardest biting animals ever, is often hypothesized to have exhibited cranial kinesis, or, mobility of cranial joints relative to the braincase. Cranial kinesis in T. rex is a biomechanical paradox in that forcefully biting tetrapods usually possess rigid skulls instead of skulls with movable joints. We tested the biomechanical performance of a tyrannosaur skull using a series of static positions mimicking possible excursions of the palate to evaluate Postural Kinetic Competency in Tyrannosaurus. A functional extant phylogenetic bracket was employed using taxa, which exhibit measurable palatal excursions: Psittacus erithacus (fore-aft movement) and Gekko gecko (mediolateral movement). Static finite element models of Psittacus, Gekko, and Tyrannosaurus were constructed and tested with different palatal postures using anatomically informed material properties, loaded with muscle forces derived from dissection, phylogenetic bracketing, and a sensitivity analysis of muscle architecture and tested in orthal biting simulations using element strain as a proxy for model performance. Extant species models showed lower strains in naturally occurring postures compared to alternatives. We found that fore-aft and neutral models of Tyrannosaurus experienced lower overall strains than mediolaterally shifted models. Protractor muscles dampened palatal strains, while occipital constraints increased strains about palatocranial joints compared to jaw joint constraints. These loading behaviors suggest that even small excursions can strain elements beyond structural failure. Thus, these postural tests of kinesis, along with the robusticity of other cranial features, suggest that the skull of Tyrannosaurus was functionally akinetic. Anat Rec, 303:999-1017, 2020. © 2019 Wiley Periodicals, Inc.
Assuntos
Força de Mordida , Dinossauros/anatomia & histologia , Fósseis , Palato/anatomia & histologia , Crânio/anatomia & histologia , Animais , Fenômenos Biomecânicos/fisiologia , Dinossauros/fisiologia , Movimento/fisiologia , Palato/fisiologia , Filogenia , Crânio/fisiologiaRESUMO
Purpose: Visual feedback therapy, using electropalatography (EPG), has been used to remedy residual articulation errors associated with cleft palate. The tongue-palate contact patterns of typical speakers without cleft palate are usually used as a model. However, it is questionable whether these model patterns are adequate for clients with repaired cleft palate, as their dento-palatal morphology is different from non-cleft speakers. The objective of this study was to investigate the differences in tongue-palate contact patterns between typical speakers with and without cleft palate.Method: EPG data were recorded for alveolar consonants in 15 participants with repaired unilateral cleft lip and palate (UCLP) whose speech was perceptually assessed as typical Japanese. The cumulative templates for each consonant were generated from the maximum contact frame; quantitative analyses (centre of gravity [CoG], variability index) were performed. Fifteen typical Japanese speakers without cleft served as a control group.Result: EPG patterns for each consonant were generally similar between groups. The CoG value of the UCLP group was significantly lower only for /s/. The average variability index was higher for every consonant but the comparisons did not reach significance.Conclusion: The typical tongue-palate contact patterns can be used as a model of visual feedback therapy.
Assuntos
Transtornos da Articulação/fisiopatologia , Fenda Labial/fisiopatologia , Fissura Palatina/fisiopatologia , Palato/fisiologia , Língua/fisiologia , Adolescente , Adulto , Transtornos da Articulação/etiologia , Povo Asiático , Fenda Labial/complicações , Fissura Palatina/complicações , Eletrofisiologia/métodos , Feminino , Humanos , Masculino , Medida da Produção da Fala/métodos , Adulto JovemRESUMO
Purpose Anterior tongue shape during /s/ production is often described as "tip-up" or apical, versus "tip-down" or laminal. Typically, this is determined by observing the shape of the anterior midline tongue. The purpose of this study was to identify methods of curvature calculation that quantify the observed shape differences and to examine whether the shape differences were affected by palate shape. Previous work shows that palate height has some effect (Grimm et al., 2017). Method Four curvature-based measures were applied to a series of points selected along the tongue surface in midsagittal cine magnetic resonance images during speech. The measures were minimal curvature, averaged largest curvature (ALC), normalized ALC, and interpolated normalized ALC. These measures were compared to visual judgments of apical and laminal /s/. Anterior palate shape was measured from dental casts. Results The apical /s/ contained a flat or concave region in the anterior tongue, while the laminal /s/ had a convex shape along the entire tongue. Thus, the laminal shape was less complex than the apical. The last 2 metrics, based on averages of multiple normalized curvatures, captured this complexity difference. Subjects with a more steeply sloped anterior palate tended to use laminal /s/. Conclusions The tongue shape for the 2 /s/ types was best defined by complexity of the shape, rather than local anterior shape. Statistical quantities that measured curvature in multiple locations, and normalized across subjects, were best at distinguishing the 2 /s/ shapes. Interpolating additional points between the manually selected ones did not improve the method. Supplemental Material https://doi.org/10.23641/asha.9733709.
Assuntos
Palato/fisiologia , Fonética , Medida da Produção da Fala/estatística & dados numéricos , Fala/fisiologia , Língua/fisiologia , Adulto , Feminino , Voluntários Saudáveis , Humanos , Imageamento por Ressonância Magnética , Masculino , Medida da Produção da Fala/métodosRESUMO
BACKGROUND AND OBJECTIVE: The orthopedic Maxillary Expansion (ME) procedure is used for treating the transverse maxillary deficiency. This pathology consists in a smaller transverse dimension in the maxilla and leads to malocclusion. The treatment takes advantage of the existence of the midpalatal suture (MPS), which corresponds to the junction at the palatine bones of its horizontal portions. The technique employs a device, conventionally a palatal expander attached to the posterior teeth, to separate the two maxillary bones in the MPS. The objective of this study was to analyze, using the Finite Element Method, the biomechanical behavior of the MPS when an expansion is applied. METHODS: A Computer Tomography image of the maxilla was reconstructed, the suture geometry was modeled with different interdigitation levels and types of hyrax devices. A total of 12 geometric models (three levels for interdigitation and four types of hyrax devices) were prepared and analyzed taking into account the chewing forces and the expansion displacement. For each case, maximum principal stresses on the maxilla (bone), and equivalent stresses on the expander device (stainless steel) were observed. In the MPS, maximum principal stresses and directional displacement were evaluated. RESULTS: The results showed that the interdigitation does not have an important influence on the deformation behavior of the maxilla but it affects the stress distribution. In addition, the type of expander device and anchorage have a direct relationship with the treatment effectiveness; larger deformation in the expansion direction was obtained with skeletal when compared to dental anchorage. CONCLUSIONS: A study that allows a better understanding of the oral biomechanics during the application of ME was presented. To our knowledge, it is the first study based on computational simulations that takes into account bone structures, like maxilla and part of the skull, to analyze the interdigitation influence on the MPS behavior when exposed to a ME.
Assuntos
Maxila/patologia , Técnica de Expansão Palatina/instrumentação , Palato/fisiologia , Fenômenos Biomecânicos , Simulação por Computador , Análise de Elementos Finitos , Humanos , Imageamento Tridimensional/métodos , Modelos Anatômicos , Estresse Mecânico , DenteRESUMO
Arrernte is a language with a relatively large consonant inventory, and a relatively small vowel inventory. In this study, electropalatography is used to examine lingual consonant production according to lexical stress, in the context of the two most common vowels in the language, the central vowels /a/ and /É/. The consonants examined are /t̪ n̪ l̪ t n l Ê É³ É É» c ɲ Ê j k Å w/. Data are for two female speakers and are taken from a large database of read texts. Results show very little articulatory variation in consonant production depending on the contexts examined. Although consonants before a stressed vowel have greater duration than consonants before an unstressed vowel, there is no consistent difference in tongue-palate contact patterns between the two prosodic contexts. The main exception to this pattern is the retroflex stop, nasal and lateral, which show a more posterior contact before an unstressed vowel: it is suggested that this is because the preceding stressed vowel contains the primary cues to retroflex place of articulation.
Assuntos
Idioma , Fonética , Acústica da Fala , Medida da Produção da Fala/métodos , Diagnóstico por Imagem/métodos , Feminino , Humanos , Palato/fisiologia , Estudos de Amostragem , Língua/fisiologiaRESUMO
Palatal shelf elevation is an essential morphogenetic process during secondary palate formation. It has been proposed that shelf elevation results from an intrinsic elevating force and is regulated by extrinsic factors that are associated with development of other orofacial structures. Although dynamic palate culture is a common in vitro approach for studying shelf elevation, it requires the tongue or the tongue and mandible to be removed before culture, which prevents any determination of the role of the extrinsic factors in regulating shelf elevation. We showed that ex vivo removal of the tongue and mandible from unfixed embryonic heads led to spontaneous shelf movements that were more pronounced at late E13.5 and early E14.5 than those of E12.5 and early E13.5, suggesting that the strength of the elevating force increases over time during palate development. We further used a suspension culture technique to analyze palatal shelf movement in an intact oral cavity by culturing the orofacial portion of embryonic heads that include the maxilla, palatal shelves, mandible, and tongue (MPMT). MPMT explants were cultured in the serum-free medium with slow rotation for 24-48 hr. The palatal shelves successfully elevated during culture and displayed intermediate morphologies that closely resemble those of in vivo shelf elevation. We demonstrate that the tongue and mandible facilitate shelf medial movement/growth during shelf elevation and further suggest that the interaction of the palatal shelves and tongue could be one of the extrinsic factors that regulate the elevation process. Anat Rec, 302:1594-1604, 2019. © 2019 American Association for Anatomy.
Assuntos
Mandíbula/fisiologia , Maxila/fisiologia , Palato/fisiologia , Língua/fisiologia , Animais , Feminino , Técnicas In Vitro , Masculino , Mandíbula/embriologia , Maxila/embriologia , Camundongos , Camundongos Endogâmicos C57BL , Morfogênese , Palato/embriologia , Língua/embriologiaRESUMO
The aim of this study was to analyze the efficacy of using rhBMP-2 (recombinant human morphogenetic protein-2) in the treatment of patients with cleft lip and palate defects (CLPD). Seven databases were screened: PubMed (Medline), Lilacs, Ibecs, Web of Science, BBO, Scopus, and The Cochrane Library. Clinical trials that evaluated the use of bioactive treatment with rhBMP-2 in the treatment of patients with CLPD were included. Statistical analyses were performed by comparing the standardized mean difference of bone formation volume and bone filling percentage (p = 0.05). Ten studies compared the use of rhBMP-2 and iliac crest bone graft (ICBG). The global analysis for bone formation volume and bone filling percentage showed that bioactive materials were similar to ICBG with a standardized mean difference of respectively 0.07 (95% CI - 0.41 to 0.56) and 0.24 (95% CI - 0.32 to 0.80). The available literature suggested that use of rhBMP-2 presented similar bone formation results to those of ICBG in secondary alveolar bone grafting for patients with CLPD.
Assuntos
Proteína Morfogenética Óssea 2/uso terapêutico , Fenda Labial/tratamento farmacológico , Fissura Palatina/tratamento farmacológico , Fator de Crescimento Transformador beta/uso terapêutico , Transplante Ósseo/métodos , Fenda Labial/epidemiologia , Fissura Palatina/epidemiologia , Humanos , Anormalidades Maxilofaciais/tratamento farmacológico , Anormalidades Maxilofaciais/epidemiologia , Palato/efeitos dos fármacos , Palato/embriologia , Palato/fisiologia , Proteínas Recombinantes/uso terapêutico , Resultado do TratamentoRESUMO
Oral mechanoreception is implicated in fundamental functions including speech, food intake and swallowing; yet, the neuroanatomical substrates that encode mechanical stimuli are not well understood. Tactile perception is initiated by intricate mechanosensitive machinery involving dedicated cells and neurons. This signal transduction setup is coupled with the topology and mechanical properties of surrounding epithelium, thereby providing a sensitive and accurate system to detect stress fluctuations from the external environment. We mapped the distribution of anatomically distinct neuronal endings in mouse oral cavity using transgenic reporters, molecular markers and quantitative histomorphometry. We found that the tongue is equipped with an array of putative mechanoreceptors that express the principal mechanosensory channel Piezo2, including end bulbs of Krause innervating individual filiform papillae and a novel class of neuronal fibers innervating the epithelium surrounding taste buds. The hard palate and gums are densely populated with three classes of sensory afferents organized in discrete patterns including Merkel cell-neurite complexes, Meissner's corpuscles and glomerular corpuscles. In aged mice, we find that palatal Merkel cells reduce in number at key time-points that correlate with impaired oral abilities, such as swallowing and mastication. Collectively, this work identifies the mechanosensory architecture of oral tissues involved in feeding.
Assuntos
Envelhecimento/fisiologia , Mucosa Bucal/citologia , Mucosa Bucal/inervação , Células Receptoras Sensoriais/citologia , Animais , Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Gengiva/citologia , Gengiva/fisiologia , Imuno-Histoquímica , Células de Merkel/citologia , Células de Merkel/fisiologia , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Mucosa Bucal/fisiologia , Palato/citologia , Palato/fisiologia , Compostos de Piridínio/farmacocinética , Compostos de Amônio Quaternário/farmacocinética , Células Receptoras Sensoriais/fisiologia , Língua/fisiologiaRESUMO
During childhood, perioral muscle function is closely associated with malocclusion. To clarify the effects of tongue function on maxillofacial morphology in children, tongue pressure and maximum lip-closing force (LCF) were measured and the relationship between perioral muscle function and maxillofacial morphology was evaluated according to the skeletal classification. Maximum tongue pressure (MTP) and swallowing tongue pressure (STP) were measured on the anterior palatine rugae in 100 children (Hellman's dental stages IIIA-IIIC) using a balloon-type tongue pressure measurement device. Lip-closing force was measured using an LCF measurement device. Lateral cephalograms were examined to classify subjects into skeletal (S)-I, S-II and S-III groups. Correlations of skeletal classification with tongue pressure and LCF were examined. Correlations of lateral cephalometric measurements with palatal volume (PV), measured using a three-dimensional optical scanner, were evaluated. Maximum tongue pressure was significantly lower in the S-II group than in other groups. Swallowing tongue pressure was significantly lower in the S-II group than in the S-III group. Lip-closing force was significantly higher in the S-III group than in other groups. Swallowing tongue pressure was positively correlated with MTP and PV. Correlations between tongue pressure and anteroposterior skeletal classification indicated the importance of quantitative tongue function assessment.
Assuntos
Cefalometria , Deglutição/fisiologia , Má Oclusão/fisiopatologia , Músculos da Mastigação/fisiologia , Contração Muscular/fisiologia , Palato/fisiologia , Língua/fisiologia , Povo Asiático , Criança , Feminino , Humanos , Lábio/fisiologia , Masculino , Músculos da Mastigação/diagnóstico por imagem , Pressão , Língua/diagnóstico por imagemRESUMO
Establishing the genetic basis that underlies craniofacial variability in natural populations is one of the main topics of evolutionary and developmental studies. One of the genes associated with mammal craniofacial variability is RUNX2, and in the present study we investigated the association between craniofacial length and width and RUNX2 across New World bats (Phyllostomidae) and primates (Catarrhini and Platyrrhini). Our results showed contrasting patterns of association between the glutamate/alanine ratios (Q/A ratio) and palate shape in these highly diverse groups. In phyllostomid bats, we found an association between shorter/broader faces and increase of the Q/A ratio. In New World monkeys (NWM) there was a positive correlation of increasing Q/A ratios to more elongated faces. Our findings reinforced the role of the Q/A ratio as a flexible genetic mechanism that would rapidly change the time of skull ossification throughout development. However, we propose a scenario in which the influence of this genetic adjustment system is indirect. The Q/A ratio would not lead to a specific phenotype, but throughout the history of a lineage, would act along with evolutionary constraints, as well as other genes, as a facilitator for adaptive morphological changes.