RESUMO
IMPORTANCE: Although the role of bovine coronavirus (BCoV) in calf diarrhea and respiratory disorders is well documented, its contribution to neurological diseases is unclear. OBJECTIVE: This study conducted virological investigations of calves showing diarrhea and respiratory and neurological signs. METHODS: An outbreak of diarrhea, respiratory, and neurological disorders occurred among the 12 calves in July 2022 in Istanbul, Türkiye. Two of these calves exhibited neurological signs and died a few days after the appearance of symptoms. One of these calves was necropsied and analyzed using molecular and histopathological tests. RESULTS: BCoV RNA was detected in the brain, lung, spleen, liver, and intestine of the calf that had neurological signs by real-time reverse transcription polymerase chain reaction. Immunostaining was also observed in the intestine and brain. A 622 bp S1 gene product was noted on gel electrophoresis only in the brain. Phylogenetic analysis indicated that the BCoV detected in this study had a high proximity to the BCoV strain GIb with 99.19% nucleotide sequence homology to the strains detected in Poland, Israel, Türkiye, and France. No distinct genetic lineages were observed when the brain isolate was compared with the respiratory and enteric strains reported to GenBank. In addition, the highest identity (98,72%) was obtained with the HECV 4408 and L07748 strains of human coronaviruses. CONCLUSIONS AND RELEVANCE: The strain detected in a calf brain belongs to the GIb-European lineage and shares high sequence homology with BCoV strains detected in Europe and Israel. In addition, the similarity between the human coronaviruses (4408 and L07748) raises questions about the zoonotic potential of the strains detected in this study.
Assuntos
Encéfalo , Doenças dos Bovinos , Infecções por Coronavirus , Coronavirus Bovino , Filogenia , Animais , Bovinos , Coronavirus Bovino/genética , Coronavirus Bovino/isolamento & purificação , Infecções por Coronavirus/veterinária , Infecções por Coronavirus/virologia , Infecções por Coronavirus/patologia , Doenças dos Bovinos/virologia , Doenças dos Bovinos/patologia , Encéfalo/virologia , Encéfalo/patologia , Turquia/epidemiologia , Surtos de Doenças/veterinária , Pneumonia Viral/veterinária , Pneumonia Viral/virologia , Pneumonia Viral/patologia , Doenças do Sistema Nervoso/veterinária , Doenças do Sistema Nervoso/virologia , Doenças do Sistema Nervoso/patologiaRESUMO
El SARS-CoV-2 es un agente patógeno que causa la enfermedad por COVID-19, la cual fue notificada por primera vez en diciembre de 2019. Se cree que el SARS-CoV-2 fue originado de una fuente animal y posteriormente diseminado a la población humana. A pesar de que se han aislado virus genéticamente relacionados en murciélagos Rhinolophus, no se ha establecido el origen exacto de SARS-CoV-2 y la ruta de introducción de este virus a la población humana sigue siendo objeto de investigación.