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1.
J Biol Chem ; 300(8): 107591, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-39032647

RESUMO

Neuronal exocytosis requires the assembly of three SNARE proteins, syntaxin and SNAP25 on the plasma membrane and synaptobrevin on the vesicle membrane. However, the precise steps in this process and the points at which assembly and fusion are controlled by regulatory proteins are unclear. In the present work, we examine the kinetics and intermediate states during SNARE assembly in vitro using a combination of time resolved fluorescence and EPR spectroscopy. We show that syntaxin rapidly forms a dimer prior to forming the kinetically stable 2:1 syntaxin:SNAP25 complex and that the 2:1 complex is not diminished by the presence of excess SNAP25. Moreover, the 2:1 complex is temperature-dependent with a reduced concentration at 37 °C. The two segments of SNAP25 behave differently. The N-terminal SN1 segment of SNAP25 exhibits a pronounced increase in backbone ordering from the N- to the C-terminus that is not seen in the C-terminal SNAP25 segment SN2. Both the SN1 and SN2 segments of SNAP25 will assemble with syntaxin; however, while the association of the SN1 segment with syntaxin produces a stable 2:2 (SN1:syntaxin) complex, the complex formed between SN2 and syntaxin is largely disordered. Synaptobrevin fails to bind syntaxin alone but will associate with syntaxin in the presence of either the SN1 or SN2 segments; however, the synaptobrevin:syntaxin:SN2 complex remains disordered. Taken together, these data suggest that synaptobrevin and syntaxin do not assemble in the absence of SNAP25 and that the SN2 segment of SNAP25 is the last to enter the SNARE complex.


Assuntos
Neurônios , Proteínas Qa-SNARE , Proteína 25 Associada a Sinaptossoma , Proteína 25 Associada a Sinaptossoma/metabolismo , Proteína 25 Associada a Sinaptossoma/genética , Proteína 25 Associada a Sinaptossoma/química , Neurônios/metabolismo , Animais , Proteínas Qa-SNARE/metabolismo , Proteínas Qa-SNARE/genética , Proteínas Qa-SNARE/química , Cinética , Proteínas SNARE/metabolismo , Proteínas SNARE/genética , Ratos , Multimerização Proteica
2.
Nat Commun ; 12(1): 6227, 2021 10 28.
Artigo em Inglês | MEDLINE | ID: mdl-34711829

RESUMO

The SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) protein syntaxin-5 (Stx5) is essential for Golgi transport. In humans, the STX5 mRNA encodes two protein isoforms, Stx5 Long (Stx5L) from the first starting methionine and Stx5 Short (Stx5S) from an alternative starting methionine at position 55. In this study, we identify a human disorder caused by a single missense substitution in the second starting methionine (p.M55V), resulting in complete loss of the short isoform. Patients suffer from an early fatal multisystem disease, including severe liver disease, skeletal abnormalities and abnormal glycosylation. Primary human dermal fibroblasts isolated from these patients show defective glycosylation, altered Golgi morphology as measured by electron microscopy, mislocalization of glycosyltransferases, and compromised ER-Golgi trafficking. Measurements of cognate binding SNAREs, based on biotin-synchronizable forms of Stx5 (the RUSH system) and Förster resonance energy transfer (FRET), revealed that the short isoform of Stx5 is essential for intra-Golgi transport. Alternative starting codons of Stx5 are thus linked to human disease, demonstrating that the site of translation initiation is an important new layer of regulating protein trafficking.


Assuntos
Anormalidades Congênitas/metabolismo , Proteínas Qa-SNARE/metabolismo , Motivos de Aminoácidos , Anormalidades Congênitas/genética , Fibroblastos/metabolismo , Glicosilação , Complexo de Golgi/metabolismo , Humanos , Mutação , Biossíntese de Proteínas , Isoformas de Proteínas/química , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Transporte Proteico , Proteínas Qa-SNARE/química , Proteínas Qa-SNARE/genética
3.
Sci Rep ; 10(1): 21350, 2020 12 07.
Artigo em Inglês | MEDLINE | ID: mdl-33288783

RESUMO

Syntaxins are a family of membrane-anchored SNARE proteins that are essential components required for membrane fusion in eukaryotic intracellular membrane trafficking pathways. Syntaxins contain an N-terminal regulatory domain, termed the Habc domain that is not highly conserved at the primary sequence level but folds into a three-helix bundle that is structurally conserved among family members. The syntaxin Habc domain has previously been found to be structurally very similar to the GAT domain present in GGA family members and related proteins that are otherwise completely unrelated to syntaxins. Because the GAT domain has been found to be a ubiquitin binding domain we hypothesized that the Habc domain of syntaxins may also bind to ubiquitin. Here, we report that the Habc domain of syntaxin 3 (Stx3) indeed binds to monomeric ubiquitin with low affinity. This domain binds efficiently to K63-linked poly-ubiquitin chains within a narrow range of chain lengths but not to K48-linked poly-ubiquitin chains. Other syntaxin family members also bind to K63-linked poly-ubiquitin chains but with different chain length specificities. Molecular modeling suggests that residues of the GGA3-GAT domain known to be important for ionic and hydrophobic interactions with ubiquitin may have equivalent, conserved residues within the Habc domain of Stx3. We conclude that the syntaxin Habc domain and the GAT domain are both structurally and functionally related, and likely share a common ancestry despite sequence divergence. Binding of Ubiquitin to the Habc domain may regulate the function of syntaxins in membrane fusion or may suggest additional functions of this protein family.


Assuntos
Proteínas Qa-SNARE/química , Proteínas Qa-SNARE/metabolismo , Sequência de Aminoácidos , Animais , Análise Mutacional de DNA/métodos , Humanos , Modelos Moleculares , Anotação de Sequência Molecular , Poliubiquitina/metabolismo , Ligação Proteica , Conformação Proteica , Proteínas SNARE/química , Proteínas SNARE/metabolismo , Ressonância de Plasmônio de Superfície
4.
Elife ; 92020 08 17.
Artigo em Inglês | MEDLINE | ID: mdl-32804076

RESUMO

Fusion of intracellular trafficking vesicles is mediated by the assembly of SNARE proteins into membrane-bridging complexes. SNARE-mediated membrane fusion requires Sec1/Munc18-family (SM) proteins, SNARE chaperones that can function as templates to catalyze SNARE complex assembly. Paradoxically, the SM protein Munc18-1 traps the Qa-SNARE protein syntaxin-1 in an autoinhibited closed conformation. Here we present the structure of a second SM-Qa-SNARE complex, Vps45-Tlg2. Strikingly, Vps45 holds Tlg2 in an open conformation, with its SNARE motif disengaged from its Habc domain and its linker region unfolded. The domain 3a helical hairpin of Vps45 is unfurled, exposing the presumptive R-SNARE binding site to allow template complex formation. Although Tlg2 has a pronounced tendency to form homo-tetramers, Vps45 can rescue Tlg2 tetramers into stoichiometric Vps45-Tlg2 complexes. Our findings demonstrate that SM proteins can engage Qa-SNAREs using at least two different modes, one in which the SNARE is closed and one in which it is open.


Assuntos
Proteínas Munc18/química , Proteínas Munc18/metabolismo , Proteínas Qa-SNARE/química , Proteínas Qa-SNARE/metabolismo , Chaetomium/genética , Proteínas Fúngicas/química , Proteínas Fúngicas/metabolismo , Ligação Proteica , Conformação Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo
5.
J Mol Biol ; 432(17): 4773-4782, 2020 08 07.
Artigo em Inglês | MEDLINE | ID: mdl-32682743

RESUMO

Membrane fusion during synaptic transmission mediates the trafficking of chemical signals and neuronal communication. The fast kinetics of membrane fusion on the order of millisecond is precisely regulated by the assembly of SNAREs and accessory proteins. It is believed that the formation of the SNARE complex is a key step during membrane fusion. Little is known, however, about the molecular machinery that mediates the formation of a large pre-fusion complex, including multiple SNAREs and accessory proteins. Syntaxin, a transmembrane protein on the plasma membrane, has been observed to undergo oligomerization to form clusters. Whether this clustering plays a critical role in membrane fusion is poorly understood in live cells. Optogenetics is an emerging biotechnology armed with the capacity to precisely modulate protein-protein interaction in time and space. Here, we propose an experimental scheme that combines optogenetics with single-vesicle membrane fusion, aiming to gain a better understanding of the molecular mechanism by which the syntaxin cluster regulates membrane fusion. We envision that newly developed optogenetic tools could facilitate the mechanistic understanding of synaptic transmission in live cells and animals.


Assuntos
Proteínas Qa-SNARE/química , Proteínas Qa-SNARE/metabolismo , Membranas Sinápticas/fisiologia , Animais , Humanos , Fusão de Membrana , Neurônios/fisiologia , Optogenética , Multimerização Proteica , Transmissão Sináptica
6.
Cells ; 8(8)2019 07 26.
Artigo em Inglês | MEDLINE | ID: mdl-31357511

RESUMO

The soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) syntaxin 5 (Stx5) in mammals and its ortholog Sed5p in Saccharomyces cerevisiae mediate anterograde and retrograde endoplasmic reticulum (ER)-Golgi trafficking. Stx5 and Sed5p are structurally highly conserved and are both regulated by interactions with other ER-Golgi SNARE proteins, the Sec1/Munc18-like protein Scfd1/Sly1p and the membrane tethering complexes COG, p115, and GM130. Despite these similarities, yeast Sed5p and mammalian Stx5 are differently recruited to COPII-coated vesicles, and Stx5 interacts with the microtubular cytoskeleton, whereas Sed5p does not. In this review, we argue that these different Stx5 interactions contribute to structural differences in ER-Golgi transport between mammalian and yeast cells. Insight into the function of Stx5 is important given its essential role in the secretory pathway of eukaryotic cells and its involvement in infections and neurodegenerative diseases.


Assuntos
Retículo Endoplasmático/metabolismo , Complexo de Golgi/metabolismo , Proteínas Qa-SNARE/metabolismo , Sequência de Aminoácidos , Animais , Humanos , Espaço Intracelular/metabolismo , Mamíferos , Modelos Moleculares , Ligação Proteica , Conformação Proteica , Isoformas de Proteínas/metabolismo , Multimerização Proteica , Processamento de Proteína Pós-Traducional , Transporte Proteico , Proteínas Qa-SNARE/química , Proteínas SNARE/metabolismo , Relação Estrutura-Atividade , Proteínas de Transporte Vesicular/metabolismo , Leveduras
7.
Sci Rep ; 9(1): 2895, 2019 02 27.
Artigo em Inglês | MEDLINE | ID: mdl-30814610

RESUMO

Type-II Diabetes Mellitus (T2DM) is one of the fastest growing public health issues of modern times, consuming 12% of worldwide health budgets and affecting an estimated 400 million people. A key pathological trait associated with this disease is the failure of normal glucose-stimulated insulin secretion (GSIS) from pancreatic beta cells. Several lines of evidence suggest that vesicle trafficking events such as insulin secretion are regulated by the post-translational modification, SUMOylation, and indeed SUMOylation has been proposed to act as a 'brake' on insulin exocytosis. Here, we show that diabetic stimuli which inhibit GSIS are correlated with an increase in cellular protein SUMOylation, and that inhibition of deSUMOylation reduces GSIS. We demonstrate that manipulation of cellular protein SUMOylation levels, by overexpression of several different components of the SUMOylation pathway, have varied and complex effects on GSIS, indicating that SUMOylation regulates this process at multiple stages. We further demonstrate that inhibition of syntaxin1A SUMOylation, via a knockdown-rescue strategy, greatly enhances GSIS. Our data are therefore consistent with the model that SUMOylation acts as a brake on GSIS, and we have identified SUMOylation of syntaxin 1 A as a potential component of this brake. However, our data also demonstrate that the role of SUMOylation in GSIS is complex and may involve many substrates.


Assuntos
Exocitose/efeitos dos fármacos , Glucose/farmacologia , Secreção de Insulina/fisiologia , Células Secretoras de Insulina/fisiologia , Proteínas Qa-SNARE/metabolismo , Sumoilação , Animais , Secreção de Insulina/efeitos dos fármacos , Células Secretoras de Insulina/citologia , Células Secretoras de Insulina/efeitos dos fármacos , Processamento de Proteína Pós-Traducional , Proteínas Qa-SNARE/química , Ratos , Edulcorantes/farmacologia
8.
J Biol Chem ; 294(11): 4188-4201, 2019 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-30655294

RESUMO

Autophagy is an intracellular degradation pathway that transports cytoplasmic material to the lysosome for hydrolysis. It is completed by SNARE-mediated fusion of the autophagosome and endolysosome membranes. This process must be carefully regulated to maintain the organization of the membrane system and prevent mistargeted degradation. As yet, models of autophagosomal fusion have not been verified within a cellular context because of difficulties with assessing protein interactions in situ Here, we used high-resolution fluorescence lifetime imaging (FLIM)-FRET of HeLa cells to identify protein interactions within the spatiotemporal framework of the cell. We show that autophagosomal syntaxin 17 (Stx17) heterotrimerizes with synaptosome-associated protein 29 (SNAP29) and vesicle-associated membrane protein 7 (VAMP7) in situ, highlighting a functional role for VAMP7 in autophagosome clearance that has previously been sidelined in favor of a role for VAMP8. Additionally, we identified multimodal regulation of SNARE assembly by the Sec1/Munc18 (SM) protein VPS33A, mirroring other syntaxin-SM interactions and therefore suggesting a unified model of SM regulation. Contrary to current theoretical models, we found that the Stx17 N-peptide appears to interact in a positionally conserved, but mechanistically divergent manner with VPS33A, providing a late "go, no-go" step for autophagic fusion via a phosphoserine master-switch. Our findings suggest that Stx17 fusion competency is regulated by a phosphosite in its N-peptide, representing a previously unknown regulatory step in mammalian autophagy.


Assuntos
Autofagia , Proteínas Qa-SNARE/metabolismo , Proteínas de Transporte Vesicular/metabolismo , Células HeLa , Humanos , Imagem Óptica , Proteínas Qa-SNARE/química , Células Tumorais Cultivadas , Proteínas de Transporte Vesicular/química
9.
Methods Mol Biol ; 1860: 115-144, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30317501

RESUMO

The interaction between the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein syntaxin (Sx) and regulatory partner Sec/Munc18 (SM) protein is a critical step in vesicle fusion. The exact role played by SM proteins, whether positive or negative, has been the topic of much debate. High-resolution structures of the SM:Sx complex have shown that SM proteins can bind syntaxin in a closed fusion incompetent state. However, in vitro and in vivo experiments also point to a positive regulatory role for SM proteins that is inconsistent with binding syntaxin in a closed conformation. Here we present protocols we used for the expression and purification of the SM proteins Munc18a and Munc18c and syntaxins 1 and 4 along with procedures used for small-angle X-ray and neutron scattering that showed that syntaxins can bind in an open conformation to SM proteins. We also describe methods for chemical cross-linking experiments and detail how this information can be combined with scattering data to obtain low-resolution structural models for SM:Sx protein complexes.


Assuntos
Proteínas Munc18/metabolismo , Ligação Proteica , Proteínas Qa-SNARE/metabolismo , Espalhamento a Baixo Ângulo , Cromatografia Líquida de Alta Pressão/instrumentação , Cromatografia Líquida de Alta Pressão/métodos , Deutério/química , Espectrometria de Massas/instrumentação , Espectrometria de Massas/métodos , Fusão de Membrana , Proteínas Munc18/química , Proteínas Munc18/isolamento & purificação , Difração de Nêutrons , Estrutura Terciária de Proteína , Proteínas Qa-SNARE/química , Proteínas Qa-SNARE/isolamento & purificação , Proteínas Recombinantes/química , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Difração de Raios X
10.
Methods Mol Biol ; 1860: 175-189, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30317504

RESUMO

Intracellular membrane fusion is mediated by the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins that are highly conserved and tightly regulated by a variety of factors. The exocyst complex is one of the multi-subunit tethering complexes and functions in the tethering of the secretory vesicles to the plasma membrane. We have found that the yeast Sec3, a subunit of the exocyst, binds to the t-SNARE protein Sso2 and promotes its interaction with another t-SNARE protein, Sec9. Here, we describe the structural analysis and in vitro membrane fusion assays, by which we found that Sec3 binding leads to a conformational change within Sso2, and facilitates SNARE assembly and the membrane fusion.


Assuntos
Fusão de Membrana , Modelos Moleculares , Proteínas Qa-SNARE/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Motivos de Aminoácidos/genética , Lipossomos/química , Lipossomos/metabolismo , Mutagênese , Ligação Proteica , Proteínas Qa-SNARE/química , Proteínas Qa-SNARE/genética , Proteínas Qa-SNARE/isolamento & purificação , Proteínas Qc-SNARE/química , Proteínas Qc-SNARE/isolamento & purificação , Proteínas Qc-SNARE/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Proteínas de Saccharomyces cerevisiae/química , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/isolamento & purificação
11.
J Phys Chem B ; 122(48): 10834-10840, 2018 12 06.
Artigo em Inglês | MEDLINE | ID: mdl-30408418

RESUMO

Neuronal transmitters are released from nerve terminals via the fusion of synaptic vesicles with the presynaptic membrane. Vesicles are attached to the membrane via the SNARE complex, comprising the vesicle associated protein synaptobrevin (Syb), the membrane associated protein syntaxin (Syx), and the cytosolic protein SNAP25, that together form a four-helical bundle. The full assembly of Syb onto the core SNARE bundle promotes vesicle fusion. We investigated SNARE assembly using a coarse-grained model of the SNARE complex that retains chemical specificity. Steered force-control simulations of SNARE unzippering were used to set up initial disassembled states of the SNARE complex. From these states, the assembly process was simulated. We find that if Syb is in helical form and proximal to the other helices, then the SNARE complex assembles rapidly, on a microsecond time-scale, which is well within in vivo synaptic vesicle fusion time scales. Assembly times grow exponentially with a separation distance between Syb and Syx C-termini. Our results indicate that for biologically relevant rapid assembly of the SNARE complex, Syb should be in helical form, and the SNARE constituent helices brought into proximity, possibly by an agent, such as a chaperone.


Assuntos
Proteínas Qa-SNARE/metabolismo , Proteínas R-SNARE/metabolismo , Proteína 25 Associada a Sinaptossoma/metabolismo , Simulação de Dinâmica Molecular , Método de Monte Carlo , Ligação Proteica , Conformação Proteica em alfa-Hélice , Dobramento de Proteína , Proteínas Qa-SNARE/química , Proteínas R-SNARE/química , Proteína 25 Associada a Sinaptossoma/química
12.
Nat Struct Mol Biol ; 25(10): 911-917, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30291360

RESUMO

The regulated exocytotic release of neurotransmitter and hormones is accomplished by a complex protein machinery whose core consists of SNARE proteins and the calcium sensor synaptotagmin-1. We propose a mechanism in which the lipid membrane is intimately involved in coupling calcium sensing to release. We found that fusion of dense core vesicles, derived from rat PC12 cells, was strongly linked to the angle between the cytoplasmic domain of the SNARE complex and the plane of the target membrane. We propose that, as this tilt angle increases, force is exerted on the SNARE transmembrane domains to drive the merger of the two bilayers. The tilt angle markedly increased following calcium-mediated binding of synaptotagmin to membranes, strongly depended on the surface electrostatics of the membrane, and was strictly coupled to the lipid order of the target membrane.


Assuntos
Exocitose , Modelos Moleculares , Sinaptotagminas/fisiologia , Vesículas Transportadoras/química , Animais , Sinalização do Cálcio , Metabolismo dos Lipídeos/fisiologia , Células PC12 , Domínios Proteicos , Proteínas Qa-SNARE/química , Proteínas Qa-SNARE/metabolismo , Proteínas Qa-SNARE/fisiologia , Ratos , Proteínas SNARE/química , Proteínas SNARE/metabolismo , Proteínas SNARE/fisiologia , Sinaptotagminas/química , Sinaptotagminas/metabolismo , Vesículas Transportadoras/metabolismo , Vesículas Transportadoras/fisiologia
13.
Mol Biol Cell ; 29(22): 2700-2708, 2018 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-30156474

RESUMO

Syntaxin (stx)-1 is an integral plasma membrane protein that is crucial for two distinct steps of regulated exocytosis, docking of secretory granules at the plasma membrane and membrane fusion. During docking, stx1 clusters at the granule docking site, together with the S/M protein munc18. Here we determined features of stx1 that contribute to its clustering at granules. In live insulin-secreting cells, stx1 and stx3 (but not stx4 or stx11) accumulated at docked granules, and stx1 (but not stx4) rescued docking in cells expressing botulinum neurotoxin-C. Using a series of stx1 deletion mutants and stx1/4 chimeras, we found that all four helical domains (Ha, Hb, Hc, SNARE) and the short N-terminal peptide contribute to recruitment to granules. However, only the Hc domain confers specificity, and it must be derived from stx1 for recruitment to occur. Point mutations in the Hc or the N-terminal peptide designed to interfere with binding to munc18-1 prevent stx1 from clustering at granules, and a mutant munc18 deficient in binding to stx1 does not cluster at granules. We conclude that stx1 is recruited to the docking site in a munc18-1-bound conformation, providing a rationale for the requirement for both proteins for granule docking.


Assuntos
Proteínas Munc18/metabolismo , Proteínas Qa-SNARE/metabolismo , Vesículas Secretórias/metabolismo , Animais , Linhagem Celular , Exocitose , Camundongos , Simulação de Acoplamento Molecular , Peptídeos/química , Ligação Proteica , Domínios Proteicos , Proteínas Qa-SNARE/química , Ratos
14.
Elife ; 72018 07 09.
Artigo em Inglês | MEDLINE | ID: mdl-29985126

RESUMO

SNARE complex disassembly by the ATPase NSF is essential for neurotransmitter release and other membrane trafficking processes. We developed a single-molecule FRET assay to monitor repeated rounds of NSF-mediated disassembly and reassembly of individual SNARE complexes. For ternary neuronal SNARE complexes, disassembly proceeds in a single step within 100 msec. We observed short- (<0.32 s) and long-lived (≥0.32 s) disassembled states. The long-lived states represent fully disassembled SNARE complex, while the short-lived states correspond to failed disassembly or immediate reassembly. Either high ionic strength or decreased αSNAP concentration reduces the disassembly rate while increasing the frequency of short-lived states. NSF is also capable of disassembling anti-parallel ternary SNARE complexes, implicating it in quality control. Finally, complexin-1 competes with αSNAP binding to the SNARE complex; addition of complexin-1 has an effect similar to that of decreasing the αSNAP concentration, possibly differentially regulating cis and trans SNARE complexes disassembly.


Assuntos
Proteínas Adaptadoras de Transporte Vesicular/metabolismo , Proteínas Sensíveis a N-Etilmaleimida/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Proteínas SNARE/metabolismo , Animais , Cricetulus , Transferência Ressonante de Energia de Fluorescência , Cinética , Camundongos , Proteínas Mutantes/metabolismo , Mutação/genética , Proteínas Sensíveis a N-Etilmaleimida/ultraestrutura , Concentração Osmolar , Ligação Proteica , Domínios Proteicos , Proteínas Qa-SNARE/química , Proteínas Qa-SNARE/metabolismo , Ratos , Imagem Individual de Molécula , Proteínas de Ligação a Fator Solúvel Sensível a N-Etilmaleimida/metabolismo , Proteínas de Ligação a Fator Solúvel Sensível a N-Etilmaleimida/ultraestrutura
15.
J Biol Chem ; 293(18): 6893-6904, 2018 05 04.
Artigo em Inglês | MEDLINE | ID: mdl-29549124

RESUMO

The voltage-dependent K+ (Kv) channel Kv2.1 is a major delayed rectifier in many secretory cells, including pancreatic ß cells. In addition, Kv2.1 has a direct role in exocytosis at an undefined step, involving SNARE proteins, that is independent of its ion-conducting pore function. Here, we elucidated the precise step in exocytosis. We previously reported that syntaxin-3 (Syn-3) is the key syntaxin that mediates exocytosis of newcomer secretory granules that spend minimal residence time on the plasma membrane before fusion. Using high-resolution total internal reflection fluorescence microscopy, we now show that Kv2.1 forms reservoir clusters on the ß-cell plasma membrane and binds Syn-3 via its C-terminal C1b domain, which recruits newcomer insulin secretory granules into this large reservoir. Upon glucose stimulation, secretory granules were released from this reservoir to replenish the pool of newcomer secretory granules for subsequent fusion, occurring just adjacent to the plasma membrane Kv2.1 clusters. C1b deletion blocked the aforementioned Kv2.1-Syn-3-mediated events and reduced fusion of newcomer secretory granules. These insights have therapeutic implications, as Kv2.1 overexpression in type-2 diabetes rat islets restored biphasic insulin secretion.


Assuntos
Células Secretoras de Insulina/metabolismo , Insulina/metabolismo , Proteínas Qa-SNARE/metabolismo , Vesículas Secretórias/metabolismo , Canais de Potássio Shab/metabolismo , Animais , Membrana Celular/metabolismo , Exocitose/fisiologia , Glucose/farmacologia , Células Secretoras de Insulina/efeitos dos fármacos , Masculino , Camundongos , Microscopia de Fluorescência , Ligação Proteica , Domínios Proteicos , Proteínas Qa-SNARE/química , Ratos , Ratos Wistar , Proteínas SNARE/metabolismo , Canais de Potássio Shab/fisiologia
16.
Prog Nucl Magn Reson Spectrosc ; 105: 41-53, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29548366

RESUMO

SNARE-mediated membrane fusion is a ubiquitous process responsible for intracellular vesicle trafficking, including membrane fusion in exocytosis that leads to hormone and neurotransmitter release. The proteins that facilitate this process are highly dynamic and adopt multiple conformations when they interact with other proteins and lipids as they form highly regulated molecular machines that operate on membranes. Solution NMR is an ideal method to capture high-resolution glimpses of the molecular transformations that take place when these proteins come together and work on membranes. Since solution NMR has limitations on the size of proteins and complexes that can be studied, lipid bilayer model membranes cannot be used in these approaches, so the relevant interactions are typically studied in various types of membrane-mimetics that are tractable by solution NMR methods. In this review we therefore first summarize different membrane-mimetic systems that are commonly used or that show promise for solution NMR studies of membrane-interacting proteins. We then summarize recent NMR studies on two SNARE proteins, syntaxin and synaptobrevin, and two related regulatory proteins, complexin and α-synuclein, and their interactions with membrane lipids. These studies provide a structural and dynamical framework for how these proteins might carry out their functions in the vicinity of lipid membranes. The common theme throughout these studies is that membrane interactions have major influences on the structural dynamics of these proteins that cannot be ignored when attempting to explain their functions in contemporary models of SNARE-mediated membrane fusion.


Assuntos
Proteínas Adaptadoras de Transporte Vesicular/química , Materiais Biomiméticos/química , Espectroscopia de Ressonância Magnética/métodos , Proteínas do Tecido Nervoso/química , Proteínas SNARE/química , alfa-Sinucleína/química , Proteínas Adaptadoras de Transporte Vesicular/metabolismo , Animais , Linhagem Celular , Membrana Celular/metabolismo , Permeabilidade da Membrana Celular , Humanos , Bicamadas Lipídicas/química , Fusão de Membrana , Proteínas do Tecido Nervoso/metabolismo , Ligação Proteica , Conformação Proteica , Transporte Proteico , Proteínas Qa-SNARE/química , Proteínas Qa-SNARE/metabolismo , Proteínas R-SNARE/química , Proteínas R-SNARE/metabolismo , Proteínas SNARE/metabolismo , alfa-Sinucleína/metabolismo
17.
J Biol Chem ; 293(10): 3593-3606, 2018 03 09.
Artigo em Inglês | MEDLINE | ID: mdl-29352103

RESUMO

Platelets regulate vascular integrity by secreting a host of molecules that promote hemostasis and its sequelae. Given the importance of platelet exocytosis, it is critical to understand how it is controlled. The t-SNAREs, SNAP-23 and syntaxin-11, lack classical transmembrane domains (TMDs), yet both are associated with platelet membranes and redistributed into cholesterol-dependent lipid rafts when platelets are activated. Using metabolic labeling and hydroxylamine (HA)/HCl treatment, we showed that both contain thioester-linked acyl groups. Mass spectrometry mapping further showed that syntaxin-11 was modified on cysteine 275, 279, 280, 282, 283, and 285, and SNAP-23 was modified on cysteine 79, 80, 83, 85, and 87. Interestingly, metabolic labeling studies showed incorporation of [3H]palmitate into the t-SNAREs increased although the protein levels were unchanged, suggesting that acylation turns over on the two t-SNAREs in resting platelets. Exogenously added fatty acids did compete with [3H]palmitate for t-SNARE labeling. To determine the effects of acylation, we measured aggregation, ADP/ATP release, as well as P-selectin exposure in platelets treated with the acyltransferase inhibitor cerulenin or the thioesterase inhibitor palmostatin B. We found that cerulenin pretreatment inhibited t-SNARE acylation and platelet function in a dose- and time-dependent manner whereas palmostatin B had no detectable effect. Interestingly, pretreatment with palmostatin B blocked the inhibitory effects of cerulenin, suggesting that maintaining the acylation state is important for platelet function. Thus, our work shows that t-SNARE acylation is actively cycling in platelets and suggests that the enzymes regulating protein acylation could be potential targets to control platelet exocytosis in vivo.


Assuntos
Plaquetas/metabolismo , Cisteína/metabolismo , Exocitose , Processamento de Proteína Pós-Traducional , Proteínas Qa-SNARE/metabolismo , Proteínas Qb-SNARE/metabolismo , Proteínas Qc-SNARE/metabolismo , Acilação/efeitos dos fármacos , Aciltransferases/antagonistas & inibidores , Aciltransferases/metabolismo , Plaquetas/efeitos dos fármacos , Plaquetas/enzimologia , Cisteína/química , Inibidores Enzimáticos/farmacologia , Exocitose/efeitos dos fármacos , Humanos , Hidroxilamina/farmacologia , Microdomínios da Membrana/efeitos dos fármacos , Microdomínios da Membrana/metabolismo , Oxirredução , Selectina-P/metabolismo , Ácido Palmítico/metabolismo , Ativação Plaquetária/efeitos dos fármacos , Agregação Plaquetária/efeitos dos fármacos , Processamento de Proteína Pós-Traducional/efeitos dos fármacos , Transporte Proteico/efeitos dos fármacos , Proteínas Qa-SNARE/química , Proteínas Qb-SNARE/química , Proteínas Qc-SNARE/química , Substâncias Redutoras/farmacologia , Propriedades de Superfície/efeitos dos fármacos , Tioléster Hidrolases/antagonistas & inibidores , Tioléster Hidrolases/metabolismo , Trítio
18.
Sci Rep ; 8(1): 193, 2018 01 09.
Artigo em Inglês | MEDLINE | ID: mdl-29317735

RESUMO

SNAREs and SNARE-binding accessory proteins are believed to be central molecular components of neurotransmitter release, although the precise sequence of molecular events corresponding to distinct physiological states is unclear. The mechanism of docking of vesicles to the plasma membrane remains elusive, as the anchoring protein residing on vesicles is unknown. Here I show that targeting small amounts of syntaxin to granules by transmembrane domain alteration leads to a substantial enhancement of syntaxin clustering beneath granules, as well as of morphological granule docking. The effect was abolished without munc18 and strongly reduced by removal of the N-terminal peptide in the syntaxin mutant. Thus, in contrast to the current paradigm, I demonstrate that syntaxin acts from the vesicular membrane, strongly facilitating docking of vesicles, likely via interaction of its N-peptide with munc18. Docking was assayed by quantifying the syntaxin clusters beneath granules, using two-color Total Internal Reflectance Fluorescence microscopy in live PC-12 cells and confirmed by electron microscopy. Hereby, I propose a new model of vesicle docking, wherein munc18 bridges the few syntaxin molecules residing on granules to the syntaxin cluster on the plasma membrane, suggesting that the number of syntaxins on vesicles determines docking and conceivably fusion probability.


Assuntos
Grânulos Citoplasmáticos/metabolismo , Proteínas Munc18/metabolismo , Proteínas Qa-SNARE/metabolismo , Animais , Sítios de Ligação , Mutação , Células PC12 , Ligação Proteica , Transporte Proteico , Proteínas Qa-SNARE/química , Proteínas Qa-SNARE/genética , Ratos
19.
J Biol Chem ; 292(50): 20449-20460, 2017 12 15.
Artigo em Inglês | MEDLINE | ID: mdl-29046354

RESUMO

The membrane fusion necessary for vesicle trafficking is driven by the assembly of heterologous SNARE proteins orchestrated by the binding of Sec1/Munc18 (SM) proteins to specific syntaxin SNARE proteins. However, the precise mode of interaction between SM proteins and SNAREs is debated, as contrasting binding modes have been found for different members of the SM protein family, including the three vertebrate Munc18 isoforms. While different binding modes could be necessary, given their roles in different secretory processes in different tissues, the structural similarity of the three isoforms makes this divergence perplexing. Although the neuronal isoform Munc18a is well-established to bind tightly to both the closed conformation and the N-peptide of syntaxin 1a, thereby inhibiting SNARE complex formation, Munc18b and -c, which have a more widespread distribution, are reported to mainly interact with the N-peptide of their partnering syntaxins and are thought to instead promote SNARE complex formation. We have reinvestigated the interaction between Munc18c and syntaxin 4 (Syx4). Using isothermal titration calorimetry, we found that Munc18c, like Munc18a, binds to both the closed conformation and the N-peptide of Syx4. Furthermore, using a novel kinetic approach, we found that Munc18c, like Munc18a, slows down SNARE complex formation through high-affinity binding to syntaxin. This strongly suggests that secretory Munc18s in general control the accessibility of the bound syntaxin, probably preparing it for SNARE complex assembly.


Assuntos
Regulação para Baixo , Modelos Moleculares , Proteínas Munc18/metabolismo , Proteínas Qa-SNARE/metabolismo , Proteínas SNARE/metabolismo , Substituição de Aminoácidos , Animais , Sítios de Ligação , Calorimetria , Cinética , Camundongos , Proteínas Munc18/química , Proteínas Munc18/genética , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/genética , Fragmentos de Peptídeos/metabolismo , Filogenia , Mutação Puntual , Conformação Proteica , Dobramento de Proteína , Domínios e Motivos de Interação entre Proteínas , Isoformas de Proteínas/química , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Multimerização Proteica , Proteínas Qa-SNARE/química , Proteínas Qa-SNARE/genética , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Proteínas SNARE/química , Termodinâmica , Titulometria
20.
J Cell Sci ; 130(22): 3829-3838, 2017 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-28972131

RESUMO

Endocytosis is crucial for all cells as it allows them to incorporate material from the extracellular space and control the availability of transmembrane proteins at the plasma membrane. In yeast, endocytosis followed by recycling to the plasma membrane results in a polarised distribution of membrane proteins by a kinetic mechanism. Here, we report that increasing the volume of residues that constitute the exoplasmic half of the transmembrane domain (TMD) in the yeast SNARE Sso1, a type II membrane protein, results in its polarised distribution at the plasma membrane. Expression of this chimera in strains affected in either endocytosis or recycling revealed that this polarisation is achieved by endocytic cycling. A bioinformatics search of the Saccharomyces cerevisiae proteome identified several proteins with high-volume exoplasmic hemi-TMDs. Our experiments indicate that TMDs from these proteins can confer a polarised distribution to the Sso1 cytoplasmic domain, indicating that the shape of the TMD can act as a novel endocytosis and polarity signal in yeast. Additionally, a high-volume exoplasmic hemi-TMD can act as an endocytosis signal in a mammalian cell line.


Assuntos
Endocitose , Sequência de Aminoácidos , Animais , Células CHO , Cricetulus , Complexos Endossomais de Distribuição Requeridos para Transporte/química , Complexos Endossomais de Distribuição Requeridos para Transporte/metabolismo , Proteínas de Membrana/química , Proteínas de Membrana/metabolismo , Domínios Proteicos , Sinais Direcionadores de Proteínas , Transporte Proteico , Proteínas Qa-SNARE/química , Proteínas Qa-SNARE/metabolismo , Saccharomyces cerevisiae , Proteínas de Saccharomyces cerevisiae/química , Proteínas de Saccharomyces cerevisiae/metabolismo , Complexos Ubiquitina-Proteína Ligase/química , Complexos Ubiquitina-Proteína Ligase/metabolismo
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