RESUMO
Mortuary practices in human evolution record cognitive, social changes and technological innovations. The Neolithic Revolution in the Levant was a watershed in this domain that has long fascinated the archaeological community. Plaster modelled skulls are well known at Jericho and several other Neolithic sites, and in Nahal Hemar cave (Israel, ca. 8200 -7300 cal. BC) excavations yielded six unique human skulls covered with a black organic coating applied in a net pattern evoking a headdress. This small cave was used as storage for paraphernalia in the semi-arid area of the Judean desert and the dry conditions preserved other artefacts such as baskets coated with a similar dark substance. While previous analysis had revealed the presence of amino acids consistent with a collagen signature, in the present report, specific biomarkers were characterised using combined proteomic and lipid approaches. Basket samples yielded collagen and blood proteins of bovine origin (Bos genus) and a large sequence coverage of a plant protein charybdin (Charybdis genus). The skull residue samples were dominated by benzoate and cinnamate derivatives and triterpenes consistent with a styrax-type resin (Styrax officinalis), thus providing the earliest known evidence of an odoriferous plant resin used in combination with an animal product.
Assuntos
Práticas Mortuárias/história , Animais , Arqueologia , Arte/história , Bovinos , Cavernas , Colágeno/química , Colágeno/história , Fósseis , História Antiga , Humanos , Israel , Práticas Mortuárias/métodos , Proteínas de Plantas/química , Proteínas de Plantas/história , CrânioRESUMO
Intraflagellar transport is the rapid, bidirectional movement of protein complexes along the length of most eukaryotic cilia and flagella. Discovery of this intracellular process in Chlamydomonas reinhardtii 20 years ago led to a rapid discovery of cellular mechanisms that underlie a large number of human ciliopathies. Described herein are the events that led to this discovery.
Assuntos
Chlamydomonas reinhardtii/metabolismo , Cílios/metabolismo , Flagelos/metabolismo , Proteínas de Plantas/história , História do Século XX , História do Século XXI , Proteínas de Plantas/metabolismo , Transporte ProteicoAssuntos
Homeopatia , Publicações Periódicas como Assunto/história , História do Século XX , Homeopatia/história , Homeopatia/métodos , Humanos , Masculino , Materia Medica/história , Oligospermia/história , Oligospermia/terapia , Extratos Vegetais/história , Proteínas de Plantas/história , Reino UnidoAssuntos
Homeopatia/história , Neoplasias/história , Antineoplásicos/história , Doença Crônica/terapia , História do Século XX , Homeopatia/métodos , Humanos , Materia Medica/história , Neoplasias/terapia , Publicações Periódicas como Assunto/história , Extratos Vegetais/história , Proteínas de Plantas/história , Reino UnidoRESUMO
After 1946, serum albumin was available for studies. Its residue sequence and internal disulfide bonding was developed by 1976. We began to make dielectric dispersion studies and apply Perrin's equations for rotational relaxation times around the two axes of revolution in 1938. These data indicated that albumin should have an elongated shape. In 1992 atomic structure data indicated the molecule was heart-shaped. A similar 1998 study of albumin complexed with fatty acid showed that the molecule was substantially rearranged. We found that the dielectric constant of albumin solutions was sensitive to fatty acid content, making this property an attractive probe in stop-flow kinetic studies. Such studies show that the fatty acid reaction is a two-step process. The fatty acid first binds to exterior sites in a diffusion-limited second order reaction complete in 1 ms. Then a first order rearrangement reaction with approximately 400 ms half-life follows. Thus the highly specialized serum albumin sequence of amino acid residues determines not only the structure of the unligated molecule, but also the distinctive structures of the numerous multiligated molecules.