RESUMO
OBJECTIVE: To investigate the volume and functional connectivity of dorsal and ventral striatal nuclei in anorexia nervosa (AN) and their relationship with early exposure to obstetric complications. METHOD: Fifty-one patients with lifetime AN (35 acute, 16 recovered) and 34 healthy controls underwent high-resolution and resting-state functional magnetic resonance imaging. RESULTS: The AN group showed reduced functional connectivity of the putamen compared with healthy women, and this reduction was more evident in patients with lifetime binge eating/purging. Both acute and recovered AN groups showed a larger left accumbens area compared with that of healthy women. The functional connectivity of bilateral nucleus accumbens and putamen showed significant negative correlations with number of obstetric complications in the AN group. DISCUSSION: This study supports the hypothesis that AN is associated with structural and functional alterations of striatal networks, and reveals the possible role of obstetric complications in the pathogenesis of striatal dysfunction.
Assuntos
Anorexia Nervosa/fisiopatologia , Efeitos Tardios da Exposição Pré-Natal/fisiopatologia , Putamen/fisiopatologia , Adulto , Idade de Início , Anorexia Nervosa/patologia , Estudos de Casos e Controles , Feminino , Humanos , Imageamento por Ressonância Magnética/métodos , Núcleo Accumbens/embriologia , Núcleo Accumbens/patologia , Núcleo Accumbens/fisiopatologia , Gravidez , Efeitos Tardios da Exposição Pré-Natal/patologia , Putamen/embriologia , Putamen/patologia , Adulto JovemRESUMO
Twin studies are one of the most powerful study designs for estimating the relative contribution of genetic and environmental influences on phenotypic variation inhuman brain morphology. In this study, we applied deformation based morphometry, a technique that provides a voxel-wise index of local tissue growth or atrophy relative to a template brain, combined with univariate ACE model, to investigate the genetic and environmental effects on the human brain structural variations in a cohort of homogeneously aged healthy pediatric twins. In addition, anatomical regions of interest (ROIs) were defined in order to explore global and regional genetic effects. ROI results showed that the influence of genetic factors on cerebrum (h(2)=0.70), total gray matter (0.67), and total white matter (0.73) volumes were significant. In particular, structural variability of left-side lobar volumes showed a significant heritability. Several subcortical structures such as putamen (h(ROI)(2)=0.79/0.77(L/R),h(MAX)(2)=0.82/0.79) and globus pallidus (0.81/0.76, 0.88/0.82) were also significantly heritable in both voxel-wise and ROI-based results. In the voxel-wise results, lateral parts of right cerebellum (c(2)=0.68) and the posterior portion of the corpus callosum (0.63) were rather environmentally determined, but it failed to reach statistical significance. Pediatric twin studies are important because they can discriminate several influences on developmental brain trajectories and identify relationships between gene and behavior. Several brain structures showed significant genetic effects and might therefore serve as biological markers for inherited traits, or as targets for genetic linkage and association studies.
Assuntos
Encéfalo/embriologia , Encéfalo/crescimento & desenvolvimento , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Variação Genética/fisiologia , Gêmeos/genética , Mapeamento Encefálico/métodos , Cerebelo/embriologia , Cerebelo/crescimento & desenvolvimento , Criança , Estudos de Coortes , Feminino , Globo Pálido/embriologia , Globo Pálido/crescimento & desenvolvimento , Humanos , Processamento de Imagem Assistida por Computador/métodos , Imageamento por Ressonância Magnética/métodos , Masculino , Putamen/embriologia , Putamen/crescimento & desenvolvimentoRESUMO
BACKGROUND: Human intrastriatal fetal allografts survive over long periods of time in the brains of Parkinson's disease (PD) patients and integrate into host circuitry. However, some grafted patients with a prior history of levodopa-induced dyskinesias have developed off-medication dyskinesias and dystonias following allografting whose mechanism remains poorly understood. The authors present single-unit discharge characteristics in the external and internal globus pallidus (GPe and GPi) in an awake patient with PD undergoing microelectrode-guided surgery for pallidal deep brain stimulation, 10 years following bilateral intraputamenal fetal mesencephalic allografting in an NIH-funded protocol. METHODS: Pallidal single-unit activity at 'rest' and during active movement was evaluated and compared with data sets from 13 PD patients in the 'off-medication' state and from one non-dyskinetic PD patient in the 'on-medication' state. RESULTS AND DISCUSSION: Analysis of firing rate, bursting discharge and oscillatory activity showed that the graft corrected some, but not all, of the abnormalities associated with the off-medication state. Additionally, in the transplanted patient, voluntary hand movement produced a marked reduction in pallidal discharge rate at multiple GPi recording sites, which was not observed during active movement in other patients. These findings are consistent with a persistent effect of transplanted dopamine cells on basal ganglia outflow and suggest a mechanism for the graft-induced dystonic phenotype.
Assuntos
Transplante de Tecido Encefálico , Transplante de Tecido Fetal , Globo Pálido/fisiopatologia , Doença de Parkinson/cirurgia , Potenciais de Ação/fisiologia , Adulto , Idoso , Estimulação Encefálica Profunda , Humanos , Pessoa de Meia-Idade , Neurônios/fisiologia , Doença de Parkinson/fisiopatologia , Tomografia por Emissão de Pósitrons , Putamen/embriologia , Putamen/transplanteRESUMO
Infants born to mothers taking selective serotonin reuptake inhibitors (SSRIs) late in pregnancy have been reported to exhibit signs of antidepressant withdrawal. Such evidence suggests that these drugs access the fetal brain in utero at biologically significant levels. Recent studies in rodents have revealed that early exposure to antidepressants can lead to long lasting abnormalities in adult behaviors, and result in robust decreases in the expression of a major serotonin synthetic enzyme (tryptophan hydroxylase) along the raphe midline. In the present investigation, we injected rat pups with citalopram (CTM: 5 mg/kg, 10 mg/kg, and 20 mg/kg) from postnatal Days 8-21, and examined serotonin transporter (SERT) labeling in the hippocampus, ventrobasal thalamic complex, and caudate-putamen when the subjects reached adulthood. Our data support the idea, that forebrain targets in receipt of innervation from the raphe midline are particularly vulnerable to the effects of CTM. SERT-immunoreactive fiber density was preferentially decreased throughout all sectors of the hippocampal formation, whereas the subcortical structures, each supplied by more lateral and rostral aspects of the raphe complex, respectively, were not significantly affected. Reductions in SERT staining were also found to be dose-dependent. These findings suggest that SSRIs may not only interfere with the establishment of chemically balanced circuits in the neonate but also impose selective impairment on higher cortical function and cognitive processes via more circumscribed (i.e., regionally specific) deficits in 5-HT action.
Assuntos
Citalopram/farmacologia , Hipocampo/efeitos dos fármacos , Hipocampo/metabolismo , Efeitos Tardios da Exposição Pré-Natal/metabolismo , Inibidores Seletivos de Recaptação de Serotonina/farmacologia , Proteínas da Membrana Plasmática de Transporte de Serotonina/metabolismo , Animais , Animais Recém-Nascidos , Núcleo Caudado/efeitos dos fármacos , Núcleo Caudado/embriologia , Núcleo Caudado/metabolismo , Córtex Cerebral/fisiologia , Cognição/fisiologia , Relação Dose-Resposta a Droga , Feminino , Hipocampo/embriologia , Modelos Animais , Gravidez , Putamen/efeitos dos fármacos , Putamen/embriologia , Putamen/metabolismo , Ratos , Ratos Long-Evans , Núcleos Ventrais do Tálamo/efeitos dos fármacos , Núcleos Ventrais do Tálamo/embriologia , Núcleos Ventrais do Tálamo/metabolismoRESUMO
Betamethasone has been used extensively to accelerate fetal lung maturation, yet little is known of its effects on neuronal morphogenesis in the developing fetus. Microtubule-associated proteins (MAPs) are a diverse family of cytoskeletal proteins that are important for brain development and the maintenance of neuroarchitecture. Vehicle (n = 7) or betamethasone (10 ug h-1, n = 7) was infused I.V. to fetal sheep over 48 h beginning at 0.87 of gestation (128 days of gestation), producing fetal plasma betamethasone concentrations resembling those to which the human fetus is exposed during antenatal glucocorticoid therapy. Paraffin sections of the left hemisphere were stained with monoclonal antibodies against MAP1B and the MAP2 isoforms MAP2a,b,c and MAP2a,b. The level of the juvenile isoform MAP2c was determined by comparison of the two MAP2 immunostainings. We were able to detect MAP1B and MAP2 immunoreactivity (IR) in the fetal sheep brain. MAP2c was the major MAP2, constituting 90.2 % of the total MAPBetamethasone exposure diminished MAP1B IR in the frontal cortex and caudate putamen (P < 0.05) but not in the hippocampus. A decrease of MAP2 IR was found in the frontal cortex, hippocampus and caudate putamen (P < 0.05). Loss of MAP2 IR was mainly due to the loss of MAP2c IR. Haematoxylin-eosin staining did not demonstrate irreversible neuronal damage. Regional cerebral blood flow determined using coloured microspheres was significantly decreased by 28 % in the frontal cortex and by 36 % in the caudate putamen but not in the hippocampus 24 h after the onset of betamethasone exposure (P < 0.05). The loss of MAP1B and MAP2a,b,c IR showed a significant correlation to the cerebral blood flow decrease only in the frontal cortex (P < 0.05). These data suggest that mechanisms other than metabolic insufficiency caused by the decreased cerebral blood flow may contribute to the loss of MAPs. The results suggest that clinical doses of betamethasone may have acute effects on cytoskeletal proteins in the fetal brain.
Assuntos
Betametasona/farmacologia , Encéfalo/embriologia , Proteínas Associadas aos Microtúbulos/metabolismo , Neurônios/metabolismo , Animais , Anticorpos Monoclonais , Betametasona/administração & dosagem , Betametasona/sangue , Encéfalo/efeitos dos fármacos , Núcleo Caudado/embriologia , Feminino , Sangue Fetal/metabolismo , Lobo Frontal/embriologia , Idade Gestacional , Hipocampo/embriologia , Imuno-Histoquímica , Infusões Intravenosas , Troca Materno-Fetal , Neurônios/efeitos dos fármacos , Gravidez , Isoformas de Proteínas/metabolismo , Putamen/embriologia , OvinosRESUMO
Knowledge of morphofunctional effects on the fetal brain induced by exogenous glucocorticoids is limited. Recently, we reported alterations of both the neuronal cytoskeleton and electrocortical function in the ovine fetal brain after antenatal betamethasone treatment in doses used in perinatal medicine. In the present study we examined whether these changes are accompanied by morphological alterations of synapses. Chronically instrumented fetal sheep at 0.87 of gestation were treated either with isotonic saline (n=7) or 10 microg/h betamethasone (n=7) over 48 h administered directly to the fetal jugular vein. Paraffin sections of the frontal neocortex, caudate putamen and hippocampus were stained with a monoclonal antibody against synaptophysin, a specific membrane protein of presynaptic vesicles and quantified morphometrically. Synaptophysin-like immunoreactivity (synaptophysin-LI) showed a widespread granular pattern in the neuropil. Betamethasone exposure reduced synaptophysin-LI in the frontal neocortex, caudate putamen and hippocampus by 46.9, 41.0 and 55.4%, respectively, (P<0.05) that was not accompanied by irreversible neuronal damage. These results suggest that clinical doses of betamethasone have acute effects on presynaptic terminals in the fetal sheep brain that could contribute to the altered complexity of electrocortical function that we have shown previously to occur following fetal exposure to betamethasone.
Assuntos
Betametasona/administração & dosagem , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Terminações Pré-Sinápticas/metabolismo , Sinaptofisina/metabolismo , Animais , Encéfalo/embriologia , Núcleo Caudado/efeitos dos fármacos , Núcleo Caudado/embriologia , Núcleo Caudado/metabolismo , Feto , Lobo Frontal/efeitos dos fármacos , Lobo Frontal/embriologia , Lobo Frontal/metabolismo , Hipocampo/efeitos dos fármacos , Hipocampo/embriologia , Hipocampo/metabolismo , Imuno-Histoquímica , Injeções Intravenosas , Neurônios/citologia , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Terminações Pré-Sinápticas/efeitos dos fármacos , Putamen/efeitos dos fármacos , Putamen/embriologia , Putamen/metabolismo , OvinosRESUMO
Within the striatum two compartments, matrix and patches, can be distinguished by differences in the expression of neuroactive substances, afferent and efferent connections and time of neurogenesis. The present study was done to demonstrate the pattern of synaptophysin (SYN) expression which is indicative of synaptogenesis in the human fetal striatum (15th-32nd weeks of gestation) with special reference to developmental changes. From the 15th to the 22nd gestational weeks an intense diffuse SYN immunolabelling of striatal patches is observed. In the matrix SYN-immunoreactive fiber bundles are seen until the 20th week. Thereafter, the matrix is nearly devoid of SYN-immunoreactive structures. From the 28th week of gestation the matrix contains diffuse SYN immunoreactivity which gradually becomes as intense as that of the patches. The latter, thus, can no longer be delineated in the 30th week. The results show that fibrous SYN immunolabelling most probably indicating intra-axonal transport of synaptic vesicles can only be observed during the first half of gestation. Moreover, it becomes obvious that the patch compartment can selectively be visualized by anti-SYN until the 28th week. This pattern may correspond to the early dopaminergic innervation from the substantia nigra which is known to reach the developing patches. From the 28th week a transition from patchy to diffuse immunolabelling is seen. The increase in matrix labelling may be due to the occurrence of new neuronal contacts. The changeover from patchy to homogeneous SYN immunolabelling takes place distinctly earlier than changes in the distribution of other neuroactive substances described before.
Assuntos
Corpo Estriado/embriologia , Desenvolvimento Embrionário e Fetal , Sinaptofisina/análise , Idoso , Idoso de 80 Anos ou mais , Núcleo Caudado/embriologia , Corpo Estriado/citologia , Feto , Idade Gestacional , Humanos , Imuno-Histoquímica , Fibras Nervosas/fisiologia , Fibras Nervosas/ultraestrutura , Putamen/embriologiaRESUMO
The developmental expression pattern of the carbohydrate epitope CD15 (Lewis X, Le X) (alpha1-->3-fucosyl-N-acetyl-lactosamine) has been immunocytochemically evaluated in paraffin sections within the human basal ganglia from 10 weeks gestation to three years after birth. At 11 weeks of gestation, CD15 (Le X) positive radial glial cells were located in the anterior and dorsal parts of the lateral ganglionic eminence. Their processes ran from the subventricular zone radially in a highly ordered fashion to the dorsolateral margin of the caudate nucleus and further to the lateral rim of the putamen. At 12 weeks of gestation, strands of CD15 (Le X) material continued to the pial surface, forming a continuous CD15 (Le X) positive borderline separating the accumbens nucleus and olfactory tubercle from the piriform cortex. At 13 weeks of gestation the dorsal putamen was completely CD15 (Le X) immunoreactive along its perimeter and CD15 (Le X) patches, consisting of fine granular material, appeared at the dorsolateral margin of the putamen at this age; while the first CD15 (Le X) patches in the caudate nucleus were observed four weeks later. The matrix compartment of the caudate and dorsal putamen became gradually stained by granular CD15 (Le X) positive material into which CD15 (Le X) immunoreactive somata were embedded. The striking contrast in staining between patch and matrix compartments disappeared shortly after birth. The ventral striatum did not become immunoreactive until the last few weeks before birth. After the formation of CD15 (Le X) positive patches in the striatum (from 12 weeks of gestation), delicate CD15 (Le X) fibres, often accumulated in bundles and related to the striatal patches, became apparent coursing towards the external pallidal lamina and the globus pallidus. Immunoreactivity in the globus pallidus itself was transient, emerging from 16 weeks of gestation, reaching a peak at 21 weeks of gestation and disappearing by birth. Both processes, i.e. the occurrence of CD15 (Le X) striatopallidal fibres and the emerging immunoreactivity in their pallidal target, may be interrelated, so that ingrowing CD15 (Le X) positive axons from the striatum provoke CD15 (Le X) expression in the external and internal pallidum. The variable patterns and intensities of CD15 (Le X) expression are possibly related to periods of maturation of the striatum and the establishment of functional interactions within the basal ganglia. Differential staining of patch and matrix in the developing neostriatum suggests that a distinct phase of cellular adhesion or dishesion mediated by the CD15 (Le X) epitope occurs during establishment of the patch and matrix regions.
Assuntos
Gânglios da Base/imunologia , Regulação da Expressão Gênica no Desenvolvimento , Antígenos CD15/genética , Envelhecimento/imunologia , Antígenos CD/análise , Antígenos CD/genética , Gânglios da Base/embriologia , Gânglios da Base/crescimento & desenvolvimento , Embrião de Mamíferos , Desenvolvimento Embrionário e Fetal/imunologia , Matriz Extracelular/fisiologia , Globo Pálido/embriologia , Globo Pálido/crescimento & desenvolvimento , Globo Pálido/imunologia , Humanos , Imuno-Histoquímica , Lactente , Recém-Nascido , Antígenos CD15/análise , Fibras Nervosas/imunologia , Fibras Nervosas/fisiologia , Putamen/embriologia , Putamen/crescimento & desenvolvimento , Putamen/imunologiaRESUMO
In order to launch a new transplantation program for Parkinson's disease (PD), we evaluated the safety and efficacy of fetal ventral mesencephalic grafts in 3 patients with advanced PD. Inclusion criteria and clinical evaluation followed strictly the Core Assessment Program for Intracerebral Transplantation. The transplantation procedure was based on the technique previously described by the groups in Lund (Sweden) and Créteil (France). The putamen contralateral to the site of predominant symptoms was unilaterally grafted in all patients. Magnetic resonance (MR)-based stereotactic guidance with multiplanar correlation was used to define 3 implantation trajectories in the precommissural, commissural, and postcommissural putamen. Fetal ventral mesencephalon was prepared from 6- to 8-week-old human embryos obtained from same-day abortions. Under general anesthesia, 8 deposits of 3 microliters of the fetal tissue were placed 1 mm apart along each implantation trajectory using a customized microsyringe and needle attached to the stereotactic frame. The patients recovered uneventfully from the neurosurgical procedure. Early postoperative MR clearly showed the implantation trajectories reaching the putamen in all patients. The follow-up period was of 12, 9 and 6 months, for each of the 3 patients, respectively. Clinical changes appeared between 3 and 6 months after transplantation and consisted of an increase in the 'on' periods and in quantitative bilateral improvement in the motor timed tests. There was an improvement of the Unified Parkinson's Disease Rating Scale score and an improvement of rigidity. Tremor was unchanged, and there was a slight and transient increase in dyskinesias. Neuropsychological follow-up revealed slight frontal alterations in 2 patients. Positron emission tomography demonstrated an increase of 18F-fluorodopa uptake in the grafted site. Adverse events include a reversible Cushing syndrome secondary to immunosuppression in 1 patient and a transient episode of confusion in another. The results of this study, designed as a prerequisite for a wider transplantation program, are in accordance with those previously reported by others and show that, using standardized neurosurgical techniques and methods of evaluation, transplantation is a reproducible and safe therapeutic approach which provides clinical benefits to patients with advanced PD.
Assuntos
Transplante de Tecido Encefálico/métodos , Transplante de Tecido Fetal/métodos , Mesencéfalo/embriologia , Doença de Parkinson/cirurgia , Putamen/embriologia , Transplante de Tecido Encefálico/normas , Protocolos Clínicos/normas , Transplante de Tecido Fetal/normas , Seguimentos , Humanos , Imageamento por Ressonância Magnética , Mesencéfalo/diagnóstico por imagem , Mesencéfalo/transplante , Período Pós-Operatório , Avaliação de Programas e Projetos de Saúde , Putamen/diagnóstico por imagem , Putamen/transplante , Técnicas Estereotáxicas , Tomografia Computadorizada de Emissão , Resultado do TratamentoRESUMO
The glycoprotein 5'-nucleotidase is a cell surface phosphatase and represents a new marker for striosomes in the adult rat caudoputamen. We report here on its developmental expression in the rat and mouse striatum, and show an unexpected converse 5'-nucleotidase chemoarchitecture of the caudoputamen in these closely related species. In the rat, 5'-nucleotidase activity was first visible as neuropil staining in tyrosine hydroxylase-positive dopamine islands of the midstriatum on postnatal day 1, and by the end of the first postnatal week, 5'-nucleotidase-positive dopamine islands also appeared rostrally. This compartmental pattern persisted thereafter, so that in adult animals, in all but the caudal caudoputamen, zones of enhanced 5'-nucleotidase staining were restricted to calbindin-D28k-poor striosomes. Weak 5'-nucleotidase activity also emerged in the matrix. In striking contrast, in the mouse striatum, enhanced 5'-nucleotidase activity was preferentially associated with extrastriosomal tissue. Enzymatic reaction first appeared on embryonic day 18, and developed over the first postnatal week into a mosaic pattern in which the matrix was stained but the dopamine islands were unstained. The matrix staining itself was heterogeneous. After the second postnatal week, most of the caudoputamen was stained, and in adult mice only rostral striosomes expressed low 5'-nucleotidase activity. We conclude that in rats, 5'-nucleotidase represents one of the few substances that maintains a preferential dopamine island/striosome distribution during striatal development. In mice, 5'-nucleotidase activity is expressed preferentially in the matrix during development, and its compartmental pattern is gradually lost with maturation, except very rostrally. These findings do not suggest an instructive role of the enzyme in striatal compartment formation in either species, but do suggest the possibility that 5'-nucleotidase contributes to the differentiation of striatal compartments during development.
Assuntos
5'-Nucleotidase/metabolismo , Núcleo Caudado/metabolismo , Dopamina/fisiologia , Glicoproteínas/biossíntese , Organelas/enzimologia , Putamen/metabolismo , 5'-Nucleotidase/imunologia , Animais , Animais Recém-Nascidos , Núcleo Caudado/embriologia , Núcleo Caudado/crescimento & desenvolvimento , Feminino , Imuno-Histoquímica , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos , Gravidez , Putamen/embriologia , Putamen/crescimento & desenvolvimento , Ratos , Ratos Sprague-Dawley , Especificidade da Espécie , Tirosina 3-Mono-Oxigenase/imunologia , Tirosina 3-Mono-Oxigenase/metabolismoRESUMO
The regional and cellular ontogeny of the mRNA encoding the dopamine- and cAMP-regulated phosphoprotein, DARPP-32, has been studied in rat striatum by quantitative in situ hybridization histochemistry. The mRNA for DARPP-32 exhibited a characteristic developmental profile. The hybridization signal was first visible on the day of birth, at which time DARPP-32 mRNA was concentrated in patches in the caudate-putamen. By the end of the first postnatal week, the majority of neurons in the caudate-putamen expressed the DARPP-32 message. Levels of mRNA per cell increased markedly during the second postnatal week, and peaked around the beginning of the third week. The adult level of DARPP-32 mRNA was lower than that observed at the apex of mRNA expression, on a per cell basis, while the proportion of neurons expressing detectable levels of message remained relatively constant. In the nucleus accumbens and olfactory tubercle, DARPP-32 mRNA development lagged somewhat behind that observed in the caudate-putamen, but was similar in other respects. A non-quantitative study employing an oligonucleotide probe complementary to the mRNA encoding another cAMP-regulated phosphoprotein, ARPP-21, revealed a similar developmental sequence to DARPP-32. The present results suggest that for DARPP-32 mRNA, genetic and, possibly, environmental factors play a role in determining the developmental patterns observed.
Assuntos
Envelhecimento/fisiologia , Núcleo Caudado/metabolismo , Regulação da Expressão Gênica , Proteínas do Tecido Nervoso/genética , Fosfoproteínas/genética , Putamen/metabolismo , RNA Mensageiro/metabolismo , Animais , Autorradiografia , Sequência de Bases , Encéfalo/metabolismo , Núcleo Caudado/embriologia , Núcleo Caudado/crescimento & desenvolvimento , DNA/genética , Fosfoproteína 32 Regulada por cAMP e Dopamina , Desenvolvimento Embrionário e Fetal , Idade Gestacional , Hibridização In Situ , Dados de Sequência Molecular , Proteínas do Tecido Nervoso/biossíntese , Oligodesoxirribonucleotídeos , Especificidade de Órgãos , Fosfoproteínas/biossíntese , Putamen/embriologia , Putamen/crescimento & desenvolvimento , RNA Mensageiro/análise , Ratos , Ratos Sprague-Dawley , Radioisótopos de EnxofreRESUMO
In the present study, we attempted to trace the development of the striatal matrix by analyzing the ontogenetic expression of calbindin-D28K (calbindin), a calcium binding protein selectivity expressed in medium-sized neurons of the matrix compartment of the mature rat's caudoputamen. The localization of calbindin was documented in a series of developing rat brains, as was the compartmental location of these cells relative to tyrosine hydroxylase (TH)-immunostained dopamine islands, sites of future striosomes. Medium-sized striatal neurons appeared in the striatum at embryonic day (E) 20, and from their first appearance, the calbindin-positive neurons had highly heterogeneous distributions. They first formed a latticework of patches and bands in a ventral region of the caudoputamen. By postnatal day (P) 7, this early calbindin-positive lattice had evolved into a mosaic in which circumscript pockets of low calbindin-like immunoreactivity appeared in more extensive calbindin-rich surrounds. With further development, the mosaic gradually encroached on all but the dorsolateral caudoputamen, a district that is calbindin-poor at adulthood. A special lateral branch of the striatal calbindin system was also identified, distinct from the rest of the calbindin-positive mosaic in several developmental characteristics. In the parts of the caudoputamen where the developing calbindin system and dopamine island system were both present, the dopamine islands invariably lay in calbindin-poor zones. Most dopamine islands, however, only filled parts of the corresponding calbindin-poor zones. Moreover, there were some calbindin-poor zones for which TH-positive dopamine islands could not be detected. Thus during development, calbindin was expressed in the extrastriosomal matrix of the striatum, but the matrix could be divided into calbindin-rich and calbindin-poor zones. In the calbindin-rich regions, there were patches of especially intense calbindin expression and zones of weaker expression. These results suggest that there is neurochemical heterogeneity in the striatal matrix during the prolonged developmental period in which the early calbindin-positive lattice expands to form the calbindin-positive matrix of the mature striatum. Surprisingly, calbindin expression in the matrix, although eventually distributed in strictly complementary fashion to striosomes, does not originate as a system complementary to dopamine islands. The prolonged disparity between the borders of dopamine islands and calbindin-poor zones, and the different spatiotemporal schedules of development of the islands and the calbindin gaps suggest instead that the final match between the borders of striosomes and surrounding matrix results from dynamic processes occurring early in postnatal development. Candidate mechanisms for the gradual adjustment of these borders are proposed.
Assuntos
Corpo Estriado/fisiologia , Neurônios/fisiologia , Proteína G de Ligação ao Cálcio S100/análise , Envelhecimento , Animais , Calbindina 1 , Calbindinas , Núcleo Caudado/embriologia , Núcleo Caudado/crescimento & desenvolvimento , Núcleo Caudado/fisiologia , Corpo Estriado/embriologia , Corpo Estriado/crescimento & desenvolvimento , Dopamina/análise , Desenvolvimento Embrionário e Fetal , Matriz Extracelular/fisiologia , Neurônios/citologia , Putamen/embriologia , Putamen/crescimento & desenvolvimento , Putamen/fisiologia , Ratos , Proteína G de Ligação ao Cálcio S100/biossíntese , Tirosina 3-Mono-Oxigenase/análiseRESUMO
The ontogeny of muscarinic cholinergic receptors in developing human brain was analyzed by in vitro receptor autoradiography with [3H]Quinuclidinyl Benzilate. It was found that muscarinic receptors develop relatively early; the levels at 24 weeks of gestation were comparable or even higher then the values in the adult brain, and that the levels of both M1 and M2 receptors increase with age. M1 receptors were concentrated mainly in forebrain regions while M2 receptors dominated in the thalamus. Scatchard analysis revealed Kd and Bmax values which are comparable to the adult values. Three brains of aborted Down's syndrome fetuses were examined in parallel and exhibited comparable levels and similar distribution to normal non-Down fetuses except for a modest increase of receptor levels which was observed in the striatum.
Assuntos
Encéfalo/embriologia , Síndrome de Down/embriologia , Receptores Muscarínicos/metabolismo , Aborto Induzido , Adulto , Encéfalo/metabolismo , Núcleo Caudado/embriologia , Síndrome de Down/metabolismo , Feminino , Lobo Frontal/embriologia , Idade Gestacional , Globo Pálido/embriologia , Hipocampo/embriologia , Humanos , Bulbo/embriologia , Especificidade de Órgãos , Gravidez , Segundo Trimestre da Gravidez , Putamen/embriologia , Quinuclidinil Benzilato/metabolismo , Valores de ReferênciaRESUMO
Specific oligonucleotide probes and in situ hybridization histochemistry were used to study the ontogeny and regulation of the mRNAs for proenkephalin A and preprosomatostatin in rat brain. In adult brain the most intense hybridization signal for the proenkephalin A mRNA was in caudate putamen, nucleus accumbens and olfactory tubercle. By contrast, the hybridization signal for preprosomatostatin mRNA was more diffusely scattered throughout the brain, with high signals in the neocortex, olfactory bulb and hippocampal formation. Studies of the ontogeny of these mRNAs revealed a different pattern of ontogenetic expression and differential regulation by dopaminergic input. The mRNA for preposomatostatin reached the highest level within the first postnatal week, whereas proenkephalin A mRNA progressively increased throughout the entire period studied. In addition the proenkephalin A mRNA showed a medial to lateral gradient in 2-day-old rat striatum which disappeared with increasing age, whereas preprosomatostatin mRNA increased in most brain areas in fairly uniform fashion with increasing age. Treatment of newborn rats with 6-hydroxydopamine increased the expression of proenkephalin A mRNA by 1.6 fold but had no effect on the expression of preprosomatostatin mRNA. The 6-hydroxydopamine-induced change in proenkephalin A mRNA expression was not observed until postnatal day 32, indicating that enkephalin-containing neurons of the developing striatum are relatively insensitive to dopamine input and that they cannot compensate for the neonatal lesion, despite the fact that the insult was given in a period of high plasticity of the neural tissue.
Assuntos
Núcleo Caudado/fisiologia , Encefalinas/genética , Precursores de Proteínas/genética , Putamen/fisiologia , RNA Mensageiro/genética , Somatostatina/genética , Envelhecimento , Animais , Núcleo Caudado/embriologia , Núcleo Caudado/crescimento & desenvolvimento , Idade Gestacional , Hibridização de Ácido Nucleico , Sondas de Oligonucleotídeos , Putamen/embriologia , Putamen/crescimento & desenvolvimento , RNA Mensageiro/análise , Ratos , Ratos EndogâmicosRESUMO
The development of the human putamen was studied using complete serial sections from the brains of 6 fetuses at 21, 23, 27, 30, 33 and 34 weeks of gestation (WG). Morphometric analysis revealed the following data. (1) The total number of putaminal neurons increased gradually from 21 to 33 WG, suggesting that the migration of putaminal neurons continues at least up to 33 WG, and the neuronal number increased markedly after 33 WG. (2) No neuronal death was observed from 21 to 34 WG. (3) Large-sized neurons 4-5 times the area of small-sized neurons appeared from 30 WG onward. The ratio of large neurons to the total putaminal neurons increased by up to 1.4% at 33 and 34 WG.
Assuntos
Putamen/embriologia , Contagem de Células , Feto/anatomia & histologia , Feto/citologia , Humanos , Neurônios/citologia , Putamen/citologiaRESUMO
By combining [3H]thymidine autoradiography with choline acetyltransferase (ChAT) immunocytochemistry, we have determined the generation pattern of the large cholinergic neurons in the neostriatum. All of these neurons are produced between embryonic days 12 and 17 (E12-E17), with 75% of them being born between E13 and 15. Cholinergic neurons appeared to be among the earliest cells produced in the neostriatum when compared with previous generation studies of all neurons in the rat caudate-putamen. The caudal-to-rostral neurogenic gradient reported in previous investigations of all neurons was the only spatiotemporal gradient observed for cholinergic neurons. The generation peak for these cells was E13 caudally, and E15 rostrally. Additional immunocytochemical studies detected ChAT immunoreactivity within somata and primary dendrites of 1 day postnatal (1 dpn) rat neostriatum, and subsequently demonstrated a dramatic increase in the intensity of reaction product and the complexity of dendritic arborizations by 14 dpn. Large ChAT-positive neurons of the basal forebrain contained within the same specimens appeared to differentiate their cholinergic phenotype earlier than those in the neostriatum. However, recent generation studies of basal forebrain neurons combined with the present results have demonstrated that both cholinergic populations are produced simultaneously along the same neurogenic gradients. This then represents an example of cholinergic projection (basal forebrain system) and local circuit (neostriatum) neurons that share similar generation patterns but differ with respect to sequences of transmitter phenotype expression. Thus, for cholinergic forebrain neurons, a cell's position along the neurogenic gradient and its transmitter phenotype appear to be more closely associated with its birth date than its ultimate projection or rate of differentiation.
Assuntos
Núcleo Caudado/embriologia , Colina O-Acetiltransferase/metabolismo , Fibras Colinérgicas/citologia , Desenvolvimento Embrionário e Fetal , Putamen/embriologia , Animais , Autorradiografia , Núcleo Caudado/citologia , Núcleo Caudado/metabolismo , Diferenciação Celular , Fibras Colinérgicas/metabolismo , Feminino , Putamen/citologia , Putamen/metabolismo , Ratos , Ratos Endogâmicos , TimidinaRESUMO
Dendritic spine density was determined quantitatively in 35-day-old neostriatal transplants and age-matched control tissue. Transplanted spiny I neurons showed significant decreases in spine density and in number of proximal dendrites. These differences may be due to aberrant maturation of transplanted neurons.
Assuntos
Núcleo Caudado/transplante , Dendritos/fisiologia , Putamen/transplante , Animais , Núcleo Caudado/citologia , Núcleo Caudado/embriologia , Sobrevivência de Enxerto , Putamen/citologia , Putamen/embriologia , RatosRESUMO
Cytodifferentiation and synaptogenesis in the neostriatum (caudate nucleus and putamen) were analyzed by the Golgi impregnation method and electron microscopy in 14 fetuses and 8 postnatal rhesus monkeys. During the second fetal month the neostriatum consists primarily of simple, mostly bipolar, immature cells and a small number of undefined profiles ending with growth cones. The first morphologically defined synapses appear in the putamen at embryonic day 60 ( E60 ) and in the head of the caudate nucleus at E65 . Synaptic density in both structures is less than one per 1000/micron2 of neuropil at this stage; synapses are characterized by asymmetric junctions between axonal profiles and immature dendritic shafts, accumulation of an intermembrane web and aggregation of round clear vesicles in presynaptic profiles. During the third fetal month neuronal cell bodies and glial cells enlarge, and axonal and dendritic processes in Golgi preparations become more complex. Although the basic morphology of synapses remains unchanged, their density increases to 9/1000 micron2 in the putamen and 3.7/1000 micron2 in the caudate. During the fourth fetal month the four principal cell classes of the neostriatum emerge. Spines on the shafts of dendrites are followed closely by the appearance of axospinous synapses. Synaptic density in the putamen is still significantly higher (10.1/1000 micron2) than in the caudate (5.4/1000 micron2), but by the end of the fifth fetal month ( E150 ) it is the same (80/1000 micron2) in both structures. A dramatic increase in synaptic density to 125/1000 micron2 occurs before term ( E165 ) with the emergence of the first asymmetric synapses as well as symmetric synapses with flat or pleomorphic vesicles that terminate predominately on dendritic shafts. Synaptic density continues to increase after birth, reaching a plateau of approximately 190/1000 micron2 at the end of the first postnatal month. Throughout postnatal development the proportions of symmetric and asymmetric synapses on the smooth dendritic shafts undergo systematic fluctuations which may reflect the ingrowth of various afferents as well as local cytological differentiation including the formation of cellular compartments.
Assuntos
Núcleo Caudado/ultraestrutura , Macaca mulatta/anatomia & histologia , Macaca/anatomia & histologia , Putamen/ultraestrutura , Sinapses/ultraestrutura , Animais , Núcleo Caudado/embriologia , Diferenciação Celular , Microscopia Eletrônica , Putamen/embriologia , Fatores de TempoRESUMO
The development of the mouse neostriatum was studied from 11 days postconception to 180 days postnatum in semithin sections, Golgi-stained sections and electron micrographs. The neostriatum was first visible in 12 day embryos. Neuroblasts, glioblasts and microglia were first identified at 13 days and by 16 days sufficient cells were differentiated to carry out differential cell counts. Astrocyte processes were identified in the electron micrographs at 15 days but astrocytes were only identified in semithin sections at birth. Oligodendrocytes were first observed 5 days after birth. No glioblasts were present later than 15 days after birth. Astrocytes were present in adult numbers by 5 days after birth; microglia were present in adult numbers by 15 days but oligodendrocytes increased in number up to 90 days. By 5 days after birth all neurons appeared fairly well differentiated in electron micrographs, but Golgi preparations showed a marked increase in dendritic spines between 5 and 90 days.
Assuntos
Núcleo Caudado/crescimento & desenvolvimento , Putamen/crescimento & desenvolvimento , Animais , Núcleo Caudado/embriologia , Núcleo Caudado/ultraestrutura , Camundongos , Microscopia Eletrônica , Neuroglia/ultraestrutura , Putamen/embriologia , Putamen/ultraestruturaRESUMO
The time of neuron origin has been determined in the basal ganglia and related basal forebrain structures of the Chinese hamster with the aid of 3H-thymidine autoradiography. Large-celled structures like the globus pallidus, nucleus of the horizontal limb of the diagonal band of Broca as well as large cells in the rostral part of the substantia innominata, in the caudate-putamen-complex and in the olfactory tubercle arise early (E12--E16), whereas medium-sized and small cells in the basal forebrain have a persistent origin over a much longer period. Neuron formation in the basal forebrain persists decrementally until P4. A clear caudorostral spatiotemporal gradient as well as a distinct 'outside-in' gradient have been observed in the caudate-putamen-complex. Medium-sized neurons in the neostriatum and in the nucleus accumbens, generated simultaneously, are usually arranged in scattered clusters. The present data on time of neuron origin strongly support other evidence which points to the conclusion that the nucleus accumbens can be considered as a ventromedial extension of the caudate-putamen-complex.