RESUMO
In the present study, we investigated the in vitro and in vivo antitumor effects of crude extract of Scutellaria Barbate (CE-SB) on mouse hepatoma H22 cells. The MTT assay was used to determine the growth inhibition of H22 cells in vitro. The in vivo therapeutic effects of CE-SB were determined using H22 tumor bearing mice. Besides, the body weight, tumor weight, thymus index and spleen index of H22 bearing mice were also measured. The tumor inhibitory rate (IR) was calculated according to the mean weight of tumor (MWT). The phagocytotic function of macrophages was examined by observing peritoneal macrophages phagocytize chicken RBC. The results showed that CE-SB could inhibit the growth of hepatoma H22 Cells in vitro and in vivo. Furthermore, CE-SB could improve immune function of H22 tumor bearing mice. Together these results indicate that CE-SB has antitumor activity and seems to be safe and effective for the use of anti-tumor therapy.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Modelos Animais de Doenças , Neoplasias Hepáticas/metabolismo , Extratos Vegetais/farmacologia , Scutellaria/química , Animais , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Leucócitos/efeitos dos fármacos , Neoplasias Hepáticas/imunologia , Macrófagos/efeitos dos fármacos , Masculino , Camundongos , Fagocitose/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Scutellaria/citologia , Scutellaria/ultraestrutura , Timo/efeitos dos fármacos , Timo/imunologia , Carga Tumoral/efeitos dos fármacos , Ensaios Antitumorais Modelo de XenoenxertoRESUMO
Methods using liquid chromatography with UV detection (LC-UV), thin-layer chromatography (TLC), and digital photomicroscopy were developed to distinguish between the different species of Scutellaria lateriflora L. and its adulterants Teucrium canadense L. and T. chamaedrys L. Chemically, the 70% ethanol extract of S. lateriflora is characterized by the presence of flavonoids--predominantly baicalin, lateriflorin, dihydrobaicalin, and baicalein. The major compounds of the 70% ethanol extract of T. canadense are phenylpropanoids, with verbascoside as the most prominent, and a variable amount of teucrioside. Teucrioside is the major compound in T. chamaedrys, but it is not present in S. lateriflora. The presence of phenylpropane glycosides can therefore be used to distinguish between the S. lateriflora L. and the two Teucrium species by LC-UV and TLC. The abundant strap-shaped trichomes on the stem, as well as bristle-like trichomes on the leaf, are typically seen microscopically for T. canadense, whereas the waxy cuticle with numerous glandular scales is found in T. chamaedrys. These cell structures were used to determine the adulteration of S. lateriflora crude herb with either of the two Teucrium species.