RESUMO
CRISPR-Cas system provides acquired immunity against invasive genetic elements in prokaryotes. In both bacteria and archaea, transcriptional factors play important roles in regulation of CRISPR adaptation and interference. In the model Crenarchaeon Sulfolobus islandicus, a CRISPR-associated factor Csa3a triggers CRISPR adaptation and activates CRISPR RNA transcription for the immunity. However, regulation of DNA repair systems for repairing the genomic DNA damages caused by the CRISPR self-immunity is less understood. Here, according to the transcriptome and reporter gene data, we found that deletion of the csa3a gene down-regulated the DNA damage response (DDR) genes, including the ups and ced genes. Furthermore, in vitro analyses demonstrated that Csa3a specifically bound the DDR gene promoters. Microscopic analysis showed that deletion of csa3a significantly inhibited DNA damage-induced cell aggregation. Moreover, the flow cytometry study and survival rate analysis revealed that the csa3a deletion strain was more sensitive to the DNA-damaging reagent. Importantly, CRISPR self-targeting and DNA transfer experiments revealed that Csa3a was involved in regulating Ups- and Ced-mediated repair of CRISPR-damaged host genomic DNA. These results explain the interplay between Csa3a functions in activating CRISPR adaptation and DNA repair systems, and expands our understanding of the lost link between CRISPR self-immunity and genome stability.
Assuntos
Proteínas Arqueais/genética , Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas , Reparo do DNA , Sulfolobus/genética , Regiões 5' não Traduzidas , Proteínas Arqueais/metabolismo , Sítios de Ligação , Proteínas Associadas a CRISPR/genética , Proteínas Associadas a CRISPR/metabolismo , Sistemas CRISPR-Cas , Dano ao DNA , Perfilação da Expressão Gênica , Regulação da Expressão Gênica em Archaea , Genoma Arqueal , Mutação , Regiões Promotoras Genéticas , Sulfolobus/crescimento & desenvolvimentoRESUMO
Aminoacyl-tRNA synthetases (aaRSs) are ancient enzymes that play a fundamental role in protein synthesis. They catalyze the esterification of specific amino acids to the 3'-end of their cognate tRNAs and therefore play a pivotal role in protein synthesis. Although previous studies suggest that aaRS-dependent errors in protein synthesis can be beneficial to some microbial species, evidence that reduced aaRS fidelity can be adaptive is limited. Using bioinformatics analyses, we identified two distinct leucyl-tRNA synthetase (LeuRS) genes within all genomes of the archaeal family Sulfolobaceae. Remarkably, one copy, designated LeuRS-I, had key amino acid substitutions within its editing domain that would be expected to disrupt hydrolytic editing of mischarged tRNALeu and to result in variation within the proteome of these extremophiles. We found that another copy, LeuRS-F, contains canonical active sites for aminoacylation and editing. Biochemical and genetic analyses of the paralogs within Sulfolobus islandicus supported the hypothesis that LeuRS-F, but not LeuRS-I, functions as an essential tRNA synthetase that accurately charges leucine to tRNALeu for protein translation. Although LeuRS-I was not essential, its expression clearly supported optimal S. islandicus growth. We conclude that LeuRS-I may have evolved to confer a selective advantage under the extreme and fluctuating environmental conditions characteristic of the volcanic hot springs in which these archaeal extremophiles reside.
Assuntos
Proteínas Arqueais/metabolismo , Leucina-tRNA Ligase/metabolismo , Sulfolobus/enzimologia , Sequência de Aminoácidos , Aminoacilação , Proteínas Arqueais/química , Proteínas Arqueais/classificação , Proteínas Arqueais/genética , Domínio Catalítico , Extremófilos/metabolismo , Edição de Genes , Concentração de Íons de Hidrogênio , Leucina/metabolismo , Leucina-tRNA Ligase/química , Leucina-tRNA Ligase/classificação , Leucina-tRNA Ligase/genética , Mutagênese Sítio-Dirigida , Filogenia , Biossíntese de Proteínas , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/química , Proteínas Recombinantes/isolamento & purificação , Alinhamento de Sequência , Sulfolobus/crescimento & desenvolvimento , TemperaturaRESUMO
Carrier state viral infection constitutes an equilibrium state in which a limited fraction of a cellular population is infected while the remaining cells are transiently resistant to infection. This type of infection has been characterized for several bacteriophages but not, to date, for archaeal viruses. Here we demonstrate that the rudivirus SIRV3 can produce a host-dependent carrier state infection in the model crenarchaeon Sulfolobus. SIRV3 only infected a fraction of a Sulfolobus islandicus REY15A culture over several days during which host growth was unimpaired and no chromosomal DNA degradation was observed. CRISPR spacer acquisition from SIRV3 DNA was induced by coinfecting with the monocaudavirus SMV1 and it was coincident with increased transcript levels from subtype I-A adaptation and interference cas genes. However, this response did not significantly affect the carrier state infection of SIRV3 and both viruses were maintained in the culture over 12 days during which SIRV3 anti-CRISPR genes were shown to be expressed. Transcriptome and proteome analyses demonstrated that most SIRV3 genes were expressed at varying levels over time whereas SMV1 gene expression was generally low. The study yields insights into the basis for the stable infection of SIRV3 and the resistance to the different host CRISPR-Cas interference mechanisms. It also provides a rationale for the commonly observed coinfection of archaeal cells by different viruses in natural environments.
Assuntos
Sistemas CRISPR-Cas/genética , Imunidade , Rudiviridae/genética , Sulfolobus/genética , Sulfolobus/imunologia , Coinfecção/virologia , DNA Viral/genética , Genoma Viral , Heterozigoto , Interações Hospedeiro-Patógeno/genética , Proteoma/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Sulfolobus/crescimento & desenvolvimento , Proteínas Virais/metabolismoRESUMO
In many organisms, hydroxyurea (HU) inhibits class I ribonucleotide reductase, leading to lowered cellular pools of deoxyribonucleoside triphosphates. The reduced levels for DNA precursors is believed to cause replication fork stalling. Upon treatment of the hyperthermophilic archaeon Sulfolobus solfataricus with HU, we observe dose-dependent cell cycle arrest, accumulation of DNA double-strand breaks, stalled replication forks, and elevated levels of recombination structures. However, Sulfolobus has a HU-insensitive class II ribonucleotide reductase, and we reveal that HU treatment does not significantly impact cellular DNA precursor pools. Profiling of protein and transcript levels reveals modulation of a specific subset of replication initiation and cell division genes. Notably, the selective loss of the regulatory subunit of the primase correlates with cessation of replication initiation and stalling of replication forks. Furthermore, we find evidence for a detoxification response induced by HU treatment.
Assuntos
Hidroxiureia/farmacologia , Ribonucleotídeo Redutases/antagonistas & inibidores , Sulfolobus/enzimologia , Proteínas de Bactérias/metabolismo , Divisão Celular/efeitos dos fármacos , DNA Primase/metabolismo , Replicação do DNA/efeitos dos fármacos , DNA Arqueal/metabolismo , Regulação da Expressão Gênica em Archaea/efeitos dos fármacos , Nucleotídeos/metabolismo , Subunidades Proteicas/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ribonucleotídeo Redutases/metabolismo , Especificidade por Substrato/efeitos dos fármacos , Sulfolobus/citologia , Sulfolobus/genética , Sulfolobus/crescimento & desenvolvimento , Transcrição Gênica/efeitos dos fármacosRESUMO
Protein post-translational methylation has been reported to occur in archaea, including members of the genus Sulfolobus, but has never been characterized on a proteome-wide scale. Among important Sulfolobus proteins carrying such modification are the chromatin proteins that have been described to be methylated on lysine side chains, resembling eukaryotic histones in that aspect. To get more insight into the extent of this modification and its dynamics during the different growth steps of the thermoacidophylic archaeon S. islandicus LAL14/1, we performed a global and deep proteomic analysis using a combination of high-throughput bottom-up and top-down approaches on a single high-resolution mass spectrometer. 1,931 methylation sites on 751 proteins were found by the bottom-up analysis, with methylation sites on 526 proteins monitored throughout three cell culture growth stages: early-exponential, mid-exponential, and stationary. The top-down analysis revealed 3,978 proteoforms arising from 681 proteins, including 292 methylated proteoforms, 85 of which were comprehensively characterized. Methylated proteoforms of the five chromatin proteins (Alba1, Alba2, Cren7, Sul7d1, Sul7d2) were fully characterized by a combination of bottom-up and top-down data. The top-down analysis also revealed an increase of methylation during cell growth for two chromatin proteins, which had not been evidenced by bottom-up. These results shed new light on the ubiquitous lysine methylation throughout the S. islandicus proteome. Furthermore, we found that S. islandicus proteins are frequently acetylated at the N terminus, following the removal of the N-terminal methionine. This study highlights the great value of combining bottom-up and top-down proteomics for obtaining an unprecedented level of accuracy in detecting differentially modified intact proteoforms. The data have been deposited to the ProteomeXchange with identifiers PXD003074 and PXD004179.
Assuntos
Proteínas Arqueais/metabolismo , Lisina/química , Proteômica/métodos , Sulfolobus/crescimento & desenvolvimento , Acetilação , Cromatina/metabolismo , Cromatografia Líquida/métodos , Metilação , Processamento de Proteína Pós-Traducional , Sulfolobus/metabolismo , Espectrometria de Massas em Tandem/métodosRESUMO
Protein methylation is believed to occur extensively in creanarchaea. Recently, aKMT, a highly conserved crenarchaeal protein lysine methyltransferase, was identified and shown to exhibit broad substrate specificity in vitro Here, we have constructed an aKMT deletion mutant of the hyperthermophilic crenarchaeon Sulfolobus islandicus The mutant was viable but showed a moderately slower growth rate than the parental strain under non-optimal growth conditions. Consistent with the moderate effect of the lack of aKMT on the growth of the cell, expression of a small number of genes, which encode putative functions in substrate transportation, energy metabolism, transcriptional regulation, stress response proteins, etc, was differentially regulated by more than twofold in the mutant strain, as compared with that in the parental strain. Analysis of the methylation of total cellular protein by mass spectrometry revealed that methylated proteins accounted for â¼2/3 (1,158/1,751) and â¼1/3 (591/1,757) of the identified proteins in the parental and the mutant strains, respectively, indicating that there is extensive protein methylation in S. islandicus and that aKMT is a major protein methyltransferase in this organism. No significant sequence preference was detected at the sites of methylation by aKMT. Methylated lysine residues, when visible in the structure, are all located on the surface of the proteins. The crystal structure of aKMT in complex with S-adenosyl-l-methionine (SAM) or S-adenosyl homocysteine (SAH) reveals that the protein consists of four α helices and seven ß sheets, lacking a substrate recognition domain found in PrmA, a bacterial homolog of aKMT, in agreement with the broad substrate specificity of aKMT. Our results suggest that aKMT may serve a role in maintaining the methylation status of cellular proteins required for the efficient growth of the organism under certain non-optimal conditions.
Assuntos
Lisina/química , Proteínas Metiltransferases/genética , Proteínas Metiltransferases/metabolismo , Proteômica/métodos , Sulfolobus/crescimento & desenvolvimento , Proteínas Arqueais/química , Proteínas Arqueais/metabolismo , Cristalografia por Raios X , Deleção de Genes , Regulação da Expressão Gênica em Archaea , Espectrometria de Massas , Metilação , Modelos Moleculares , Proteínas Metiltransferases/química , Estrutura Secundária de Proteína , S-Adenosil-Homocisteína/metabolismo , S-Adenosilmetionina/metabolismo , Sulfolobus/genética , Sulfolobus/metabolismoRESUMO
DExD/H-box helicases represent the largest family of helicases. They belong to superfamily 2 helicases and participate in nucleotide metabolism, ribosome biogenesis, and nucleocytoplasmic transport. The biochemical properties and structures of some DExD/H-box helicases in the archaea have been documented, but many of them have not been characterized; and reports on in vivo functional analyses are limited. In this study, we attempted gene knockout of 8 putative DExD/H-box helicases in Sulfolobus islandicus REY15A and obtained two deletion mutants, SiRe_0681 and SiRe_1605. We determined that ΔSiRe_0681 grew faster than wild type cells in the presence of methyl methanesulfonate (MMS). Flow cytometry analysis showed that this strain had fewer G1/S phase cells than the wild type, and the genes coding for cell division proteins were up-regulated. The stain ΔSiRe_1605 was more sensitive to MMS than the wild type cell, and many nucleotide metabolism and DNA repair enzymes were found to be down-regulated. Intriguingly, deletion of either gene led to silencing simultaneously of over 80 genes located at a specific region. This study provides a novel insight into the in vivo functions of predicted DExD/H-box family helicases in the archaea.
Assuntos
Proteínas Arqueais/genética , DNA Helicases/genética , Deleção de Genes , Sulfolobus/enzimologia , Proteínas Arqueais/metabolismo , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , DNA Helicases/metabolismo , Enzimas Reparadoras do DNA/genética , Enzimas Reparadoras do DNA/metabolismo , Fase G1 , Sulfolobus/genética , Sulfolobus/crescimento & desenvolvimentoRESUMO
A transcriptome study was performed on Sulfolobus islandicus REY15A actively undergoing CRISPR spacer acquisition from the crenarchaeal monocaudavirus STSV2 in rich and basal media over a 6 day period. Spacer acquisition preceded strong host growth retardation, altered transcriptional activity of four different CRISPR-Cas modules and changes in viral copy numbers, and with significant differences in the two media. Transcript levels of proteins involved in the cell cycle were reduced, whereas those of DNA replication, DNA repair, transcriptional regulation and some antitoxin-toxin pairs and transposases were unchanged or enhanced. Antisense RNAs were implicated in the transcriptional regulation of adaptation and interference modules of the type I-A CRISPR-Cas system, and evidence was found for the occurrence of functional co-ordination between the single CRISPR-Cas adaptation module and the functionally diverse interference modules.
Assuntos
Vírus de Archaea/genética , Sistemas CRISPR-Cas , Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas , Sulfolobus/genética , Sulfolobus/virologia , Transcriptoma , Replicação do DNA/genética , DNA Viral/genética , Regulação da Expressão Gênica em Archaea , Interações Hospedeiro-Patógeno , Dados de Sequência Molecular , Sulfolobus/crescimento & desenvolvimento , Ativação Transcricional , Replicação Viral/genéticaRESUMO
Biofilms are surface-associated colonies of microorganisms embedded in a matrix of extracellular polymeric substances (EPS). As EPS mediate the contact between cells and surfaces, an understanding of their composition and production is of particular interest. In this study, the EPS components of Sulfolobus metallicus DSM 6482(T) forming biofilms on elemental sulfur (S(0)) were investigated by confocal laser scanning microscopy (CLSM). In order to visualize cell and EPS distributions, biofilm cells were stained with various dyes specific for glycoconjugates, proteins, nucleic acids and lipids. Biofilm cells on S(0) were heterogeneously distributed and characterized as individual cells, microcolonies, and large clusters up to a hundred micrometers in diameter. The glycoconjugates in biofilms were detected by fluorescence lectin-binding analysis (FLBA). Screening of 72 commercially available lectins resulted in the selection of 21 lectins useful for staining biofilms of S. metallicus (T). Capsular EPS from planktonic cells were mainly composed of carbohydrates and proteins. In contrast, colloidal EPS from planktonic cells were dominated by carbohydrates. Proteins were found to be major components in EPS from biofilms on S(0). Using specific probes combined with CLSM, we showed that extracellular proteins and nucleic acids were present in the EPS matrix. Finally, we showed that S. metallicus (T) cells were embedded in a flexible EPS matrix. This study provides new insights into archaeal biofilms and EPS composition and properties with respect to their interactions with S(0).
Assuntos
Biopolímeros/análise , Glicoconjugados/análise , Sulfolobus/química , Biofilmes/crescimento & desenvolvimento , Carboidratos/análise , Lectinas/metabolismo , Microscopia Confocal , Ligação Proteica , Proteínas/análise , Coloração e Rotulagem , Sulfolobus/crescimento & desenvolvimento , Sulfolobus/fisiologia , EnxofreRESUMO
UNLABELLED: We investigated the interaction between Sulfolobus spindle-shaped virus (SSV9) and its native archaeal host Sulfolobus islandicus. We show that upon exposure to SSV9, S. islandicus strain RJW002 has a significant growth delay where the majority of cells are dormant (viable but not growing) for 24 to 48 hours postinfection (hpi) compared to the growth of controls without virus. We demonstrate that in this system, dormancy (i) is induced by both active and inactive virus particles at a low multiplicity of infection (MOI), (ii) is reversible in strains with active CRISPR-Cas immunity that prevents the establishment of productive infections, and (iii) results in dramatic and rapid host death if virus persists in the culture even at low levels. Our results add a new dimension to evolutionary models of virus-host interactions, showing that the mere presence of a virus induces host cell stasis and death independent of infection. This novel, highly sensitive, and risky bet-hedging antiviral response must be integrated into models of virus-host interactions in this system so that the true ecological impact of viruses can be predicted and understood. IMPORTANCE: Viruses of microbes play key roles in microbial ecology; however, our understanding of viral impact on host physiology is based on a few model bacteria that represent a small fraction of the life history strategies employed by hosts or viruses across the three domains that encompass the microbial world. We have demonstrated that rare and even inactive viruses induce dormancy in the model archaeon S. islandicus. Similar virus-induced dormancy strategies in other microbial systems may help to explain several confounding observations in other systems, including the surprising abundance of dormant cell types found in many microbial environments, the difficulty of culturing microorganisms in the laboratory, and the paradoxical virus-to-host abundances that do not match model predictions. A more accurate grasp of virus-host interactions will expand our understanding of the impact of viruses in microbial ecology.
Assuntos
Interações Hospedeiro-Parasita , Viabilidade Microbiana , Sulfolobus/crescimento & desenvolvimento , Sulfolobus/virologia , Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas , Fatores de TempoRESUMO
BACKGROUND: ATPase/Helicases and nucleases play important roles in homologous recombination repair (HRR). Many of the mechanistic details relating to these enzymes and their function in this fundamental and complicated DNA repair process remain poorly understood in archaea. Here we employed Sulfolobus islandicus, a hyperthermophilic archaeon, as a model to investigate the in vivo functions of the ATPase/helicase HerA, the nuclease NurA, and their associated proteins Mre11 and Rad50. RESULTS: We revealed that each of the four genes in the same operon, mre11, rad50, herA, and nurA, are essential for cell viability by a mutant propagation assay. A genetic complementation assay with mutant proteins was combined with biochemical characterization demonstrating that the ATPase activity of HerA, the interaction between HerA and NurA, and the efficient 5'-3' DNA end resection activity of the HerA-NurA complex are essential for cell viability. NurA and two other putative HRR proteins: a PIN (PilT N-terminal)-domain containing ATPase and the Holliday junction resolvase Hjc, were co-purified with a chromosomally encoded N-His-HerA in vivo. The interactions of HerA with the ATPase and Hjc were further confirmed by in vitro pull down. CONCLUSION: Efficient 5'-3' DNA end resection activity of the HerA-NurA complex contributes to necessity of HerA and NurA in Sulfolobus, which is crucial to yield a 3'-overhang in HRR. HerA may have additional binding partners in cells besides NurA.
Assuntos
DNA Helicases/metabolismo , Desoxirribonucleases/metabolismo , Genes Essenciais , Sulfolobus/crescimento & desenvolvimento , Proteínas Arqueais/genética , Proteínas Arqueais/metabolismo , DNA Helicases/genética , DNA Arqueal/metabolismo , Desoxirribonucleases/genética , Endodesoxirribonucleases/genética , Endodesoxirribonucleases/metabolismo , Exodesoxirribonucleases/genética , Exodesoxirribonucleases/metabolismo , Óperon , Reparo de DNA por Recombinação , Sulfolobus/enzimologia , Sulfolobus/genéticaRESUMO
Sulfolobus species have become the model organisms for studying the unique biology of the crenarchaeal division of the archaeal domain. In particular, Sulfolobus islandicus provides a powerful opportunity to explore natural variation via experimental functional genomics. To support these efforts, we further expanded genetic tools for S. islandicus by developing a stringent positive selection for agmatine prototrophs in strains in which the argD gene, encoding arginine decarboxylase, has been deleted. Strains with deletions in argD were shown to be auxotrophic for agmatine even in nutrient-rich medium, but growth could be restored by either supplementation of exogenous agmatine or reintroduction of a functional copy of the argD gene from S. solfataricus P2 into the ΔargD host. Using this stringent selection, a robust targeted gene knockout system was established via an improved next generation of the MID (marker insertion and unmarked target gene deletion) method. Application of this novel system was validated by targeted knockout of the upsEF genes involved in UV-inducible cell aggregation formation.
Assuntos
Agmatina/metabolismo , Marcação de Genes/métodos , Genética Microbiana/métodos , Biologia Molecular/métodos , Seleção Genética , Sulfolobus/genética , Sulfolobus/metabolismo , Carboxiliases/genética , Deleção de Genes , Técnicas de Inativação de Genes , Teste de Complementação Genética , Sulfolobus/crescimento & desenvolvimentoRESUMO
The endosomal-sorting complex required for transport (ESCRT) is evolutionarily conserved from Archaea to eukaryotes. The complex drives membrane scission events in a range of processes, including cytokinesis in Metazoa and some Archaea. CdvA is the protein in Archaea that recruits ESCRT-III to the membrane. Using electron cryotomography (ECT), we find that CdvA polymerizes into helical filaments wrapped around liposomes. ESCRT-III proteins are responsible for the cinching of membranes and have been shown to assemble into helical tubes in vitro, but here we show that they also can form nested tubes and nested cones, which reveal surprisingly numerous and versatile contacts. To observe the ESCRT-CdvA complex in a physiological context, we used ECT to image the archaeon Sulfolobus acidocaldarius and observed a distinct protein belt at the leading edge of constriction furrows in dividing cells. The known dimensions of ESCRT-III proteins constrain their possible orientations within each of these structures and point to the involvement of spiraling filaments in membrane scission.
Assuntos
Proteínas Arqueais/metabolismo , Complexos Endossomais de Distribuição Requeridos para Transporte/metabolismo , Sulfolobus/crescimento & desenvolvimento , Proteínas Arqueais/ultraestrutura , Membrana Celular/fisiologia , Microscopia Crioeletrônica , Citocinese , Tomografia com Microscopia Eletrônica , Complexos Endossomais de Distribuição Requeridos para Transporte/ultraestrutura , Sulfolobus/metabolismo , Sulfolobus/ultraestruturaRESUMO
The spectroscopic properties of thermophilic cytochrome P450 from the thermoacidophilic crenarchaeon Sulfolobus tokodaii strain 7 (P450st) were investigated in acidic and basic solutions. The resting form of ferric-P450st in weakly-acidic and neutral solutions contained a thiolate/H2O coordinated low-spin heme. Below pH 1.5, P450st underwent cleavage of the Fe-S bond and was converted into apo-P450st. Above pH 8, the acid-alkaline transition due to the deprotonation of the water ligand was observed. The produced thiolate/OHâ»-coordinated ferric-P450st was stable at room temperature. The pK(a) value of 8.7 for the transition reflects the protonation properties of the distal side of the heme.
Assuntos
Proteínas Arqueais/metabolismo , Sistema Enzimático do Citocromo P-450/metabolismo , Sulfolobus/enzimologia , Sulfolobus/crescimento & desenvolvimento , Apoproteínas/química , Apoproteínas/metabolismo , Proteínas Arqueais/química , Proteínas Arqueais/genética , Dicroísmo Circular , Sistema Enzimático do Citocromo P-450/química , Sistema Enzimático do Citocromo P-450/genética , Espectroscopia de Ressonância de Spin Eletrônica , Estabilidade Enzimática , Heme/química , Heme/metabolismo , Concentração de Íons de Hidrogênio , Cinética , Oxirredução , Conformação Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Espectrofotometria , Sulfolobus/metabolismoRESUMO
Acanthamoeba isolation from extreme environments suggests that they may play a role in regulating archaeal densities and contribute to these ecosystems. The purpose of this study was to determine whether Acanthamoeba grow on extremophilic/mesophilic Archaea that are dominant cellular organisms in such environments. Sulfolobus solfataricus P2 and Sulfolobus shibatae were used as representative of Archaea, while Escherichia coli K-12 strain HB101 was used as a positive control for amoeba growth. Acanthamoeba castellanii were inoculated on nonnutrient agar plates containing lawns of Sulfolobus and E. coli. The cultures of Sulfolobus supported A. castellanii growth similar to E. coli K-12, HB101. Overall, the findings revealed that Acanthamoeba feed on Sulfolobus, which may explain amoebae presence in extreme environments. This feeding behavior is important as extremophilic/mesophilic Archaea are known to play a role in biogeochemical cycling of different elements in their natural habitat impacting different ecosystems.
Assuntos
Acanthamoeba/crescimento & desenvolvimento , Interações Microbianas , Sulfolobus/crescimento & desenvolvimento , Acanthamoeba/metabolismo , Escherichia coli K12/crescimento & desenvolvimentoRESUMO
In the tank bioleaching process, maximising solid loading and mineral availability, the latter through decreasing particle size, are key to maximising metal extraction. In this study, the effect of particle size distribution on bioleaching performance and microbial growth was studied through applying knowledge based on medical geology research to understand the adverse effects of suspended fine pyrite particles. Small-scale leaching studies, using pyrite concentrate fractions (106-75, 75-25, -25 µm fines), were used to confirm decreasing performance with decreasing particle size (D 50 <40 µm). Under equivalent experimental conditions, the generation of the reactive oxygen species (ROS), hydrogen peroxide and hydroxyl radicals from pyrite was illustrated. ROS generation measured from the different pyrite fractions was found to increase with increasing pyrite surface area loading (1.79-74.01 m(2) L(-1)) and Fe(2+) concentration (0.1-2.8 g L(-1)) in solution. The highest concentration of ROS was measured from the finest fraction of pyrite (0.85 mM) and from the largest concentration of Fe(2+) (0.78 mM). No ROS was detected from solutions containing only Fe(3+) under the same conditions tested. The potential of ROS to inhibit microbial performance under bioleaching conditions was demonstrated. Pyrite-free Sulfolobus metallicus cultures challenged with hydrogen peroxide (0.5-2.5 mM) showed significant decrease in both cell growth and Fe(2+) oxidation rates within the concentration range 1.5-2.5 mM. In combination, the results from this study suggest that conditions of large pyrite surface area loading, coupled with high concentrations of dissolved Fe(2+), can lead to the generation of ROS, resulting in oxidative stress of the microorganisms.
Assuntos
Biotecnologia/métodos , Ferro/metabolismo , Minerais/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Sulfetos/metabolismo , Sulfolobus/crescimento & desenvolvimento , Sulfolobus/metabolismo , Microbiologia Ambiental , Sulfolobus/efeitos dos fármacosRESUMO
Environmental organisms are extremely diverse and only a small fraction has been successfully cultured in the laboratory. Culture in micro wells provides a method for rapid screening of a wide variety of growth conditions and commercially available plates contain a large number of substrates, nutrient sources, and inhibitors, which can provide an assessment of the phenotype of an organism. This review describes applications of phenotype arrays to anaerobic and thermophilic microorganisms, use of the plates in stress response studies, in development of culture media for newly discovered strains, and for assessment of phenotype of environmental communities. Also discussed are considerations and challenges in data interpretation and visualization, including data normalization, statistics, and curve fitting.
Assuntos
Archaea/crescimento & desenvolvimento , Bactérias/crescimento & desenvolvimento , Microbiologia Ambiental , Análise em Microsséries/métodos , Fenótipo , Archaea/isolamento & purificação , Archaea/metabolismo , Bactérias/isolamento & purificação , Bactérias/metabolismo , Meios de Cultura , Geobacter/crescimento & desenvolvimento , Geobacter/isolamento & purificação , Geobacter/metabolismo , Humanos , Poluição por Petróleo , Filogenia , Sulfolobus/crescimento & desenvolvimento , Sulfolobus/isolamento & purificação , Sulfolobus/metabolismoRESUMO
We report here a novel selectable marker for the hyperthermophilic crenarchaeon Sulfolobus islandicus. The marker cassette is composed of the sac7d promoter and the hmg gene coding for the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (P(sac7d)-hmg), which confers simvastatin resistance to this crenarchaeon. The basic plasmid vector pSSR was constructed by substituting the pyrEF gene of the expression vector pSeSD for P(sac7d)-hmg with which the Sulfolobus expression plasmids pSSRlacS, pSSRAherA, and pSSRNherA were constructed. Characterization of Sulfolobus transformants carrying pSSRlacS indicated that the plasmid was properly maintained under selection. High-level expression of the His(6)-tagged HerA helicase was obtained with the cells harboring pSSRAherA. The establishment of two efficient selectable markers (pyrEF and hmg) was subsequently exploited for genetic analysis. A herA merodiploid strain of S. islandicus was constructed using pyrEF marker and used as the host to obtain pSSRNherA transformant with simvastatin selection. While the gene knockout (ΔherA) cells generated from the herA merodiploid cells failed to form colonies in the presence of 5-fluoroorotic acid (5-FOA), the mutant cells could be rescued by expression of the gene from a plasmid (pSSRNherA), because their transformants formed colonies on a solid medium containing 5-FOA and simvastatin. This demonstrates that HerA is essential for cell viability of S. islandicus. To our knowledge, this is the first application of an antibiotic selectable marker in genetic study for a hyperthermophilic acidophile and in the crenarchaeal lineage.
Assuntos
Resistência a Medicamentos , Hidroximetilglutaril-CoA-Redutases NADP-Dependentes/metabolismo , Seleção Genética , Sinvastatina/metabolismo , Sinvastatina/toxicidade , Sulfolobus/crescimento & desenvolvimento , Sulfolobus/metabolismo , Biologia Molecular/métodosRESUMO
Sulfolobus metallicus is a hyperthermophilic and chemolithoautotrophic archaeon that uses elemental sulfur as an energy source. Its ability to oxidize H(2)S was measured either in the presence or absence of elemental sulphur, showing its ability for using both as an energy source. A biotrickling filter was set up and a biofilm of S. metallicus was established over the support. The maximum removal capacity of the biotrickling filter reached at 55°C was 40 g S/m(3)h for input loads higher than 70 g S/m(3)h. Thus, S. metallicus can be used in a biofiltration system for the treatment of waste gas emissions at high temperatures contaminated with H(2)S.
Assuntos
Sulfeto de Hidrogênio/metabolismo , Sulfolobus/metabolismo , Biofilmes/crescimento & desenvolvimento , Filtração/métodos , Oxirredução , Sulfolobus/crescimento & desenvolvimento , Sulfolobus/fisiologia , Enxofre/metabolismo , Temperatura , Purificação da Água/métodosRESUMO
Topoisomerase III (topo III), a type IA topoisomerase, is widespread in hyperthermophilic archaea. In order to interrogate the in vivo role of archaeal topo III, we constructed and characterized a topo III gene deletion mutant of Sulfolobus islandicus. The mutant was viable but grew more slowly than the wild-type strain, especially in a nutrient-poor medium. Flow cytometry analysis revealed changes of the mutant in growth cycle characteristics including an increase in proportion of cells containing either more than two genome equivalents or less than one genome equivalent in exponentially-growing cultures. As shown by fluorescence microscopy, a fraction of mutant cells in the cultures were drastically enlarged, and at least some of the enlarged cells were apparently capable of resuming cell division. The mutant also shows a different transcriptional profile from that of the wild-type strain. Our results suggest that the enzyme may serve roles in chromosomal segregation and control of the level of supercoiling in the cell.