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Cytotoxicity, Morphology and Chemical Composition of Two Luting Cements: An in Vitro Study
Said, Farrukh; Moeen, Faisal; Khan, Muhammad Talal; Mansoor, Afsheen; Uzbek, Usman Haider; Alam, Mohammad Khursheed; Siddiqui, Ammar Ahmed.
Afiliação
  • Said, Farrukh; Islamic International Dental College. Department of Dental Materials. Islamabad. PK
  • Moeen, Faisal; Islamic International Dental College. Department of Dental Materials. Islamabad. PK
  • Khan, Muhammad Talal; Islamic International Dental College. Department of Dental Materials. Islamabad. PK
  • Mansoor, Afsheen; Islamic International Dental College. Department of Dental Materials. Islamabad. PK
  • Uzbek, Usman Haider; s.af
  • Alam, Mohammad Khursheed; Jouf University. College of Dentistry. Department of Orthodontics. Sakaka. SA
  • Siddiqui, Ammar Ahmed; University of Hail. College of Dentistry. Department of Preventive Dentistry. Hail. SA
Article em En | LILACS, BBO | ID: biblio-1101284
Biblioteca responsável: BR1264.1
ABSTRACT
Abstract

Objective:

To assess the cytotoxicity, surface morphology, elemental compositions and chemical characterization of two commonly used luting cement. Material and

Methods:

The two luting types of cement used were Elite Cement® and Hy-Bond Resiglass®. Freshly mixed (n=6) and set form (n=6) of each cement was placed in medium to obtain extracts. The extract from each sample was exposed to L929 mouse fibroblasts (1x104cells/well). Alamar Blue Assay assessed cell viability. Surface morphology and elemental composition were evaluated using scanning electron microscopy and energy dispersive spectroscopy. The chemical characterization was performed by Fourier Transform Infrared Spectroscopy. One-way ANOVA and post-hoc Tukey analysis were conducted to assess results.

Results:

Hy-Bond Resiglass® was the more cytotoxic of the two types of cement in both freshly mixed (68.10 +5.16; p<0.05) and set state (87.58 +4.86; p<0.05), compared to Elite Cement® both freshly mixed (77.01 +5.45; p<0.05) and set state (89.39 +5.66; p<0.05). Scanning electron microscopy revealed a more irregular and porous structure in Hy-Bond Resiglass® compared to Elite Cement®. Similarly, intense peaks of aluminium, tungsten and fluorine were observed in energy dispersive spectroscopy in Hy-Bond Resiglass.

Conclusion:

All these three elements (aluminium, tungsten and fluorine) have cytotoxic potential. The Fourier transform infrared spectroscopy revealed the presence of hydroxyethyl methacrylate in Hy-Bond Resiglass®, which has a cytotoxic potential.
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Texto completo: 1 Base de dados: BBO / LILACS Assunto principal: Cimento de Fosfato de Zinco / Técnicas In Vitro / Cimentos Dentários / Fibroblastos Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: BBO / LILACS Assunto principal: Cimento de Fosfato de Zinco / Técnicas In Vitro / Cimentos Dentários / Fibroblastos Idioma: En Ano de publicação: 2020 Tipo de documento: Article