Hypoxia inducible factor-1 (HIF-1α) reduced inflammation in spinal cord injury via miR-380-3p/ NLRP3 by Circ 0001723
Biol. Res
; 53: 35, 2020. graf
Article
em En
| LILACS
| ID: biblio-1131881
Biblioteca responsável:
CL1.1
ABSTRACT
BACKGROUND:
Spinal cord injury (SCI) is a severe central nervous system trauma. The present study aimed to evaluate the effect of HIF-1α on inflammation in spinal cord injury (SCI) to uncover the molecular mechanisms of anti-inflammation.RESULTS:
HIF-1α was reduced in SCI model rats and HIF-1α activation reduced TNF-α, IL-1ß, IL-6 and IL-18 levels in SCI model rats. Meanwhile, Circ 0001723 expression was down-regulated and miR-380-3p expression was up-regulated in SCI model rats. In vitro model, down-regulation of Circ 0001723 promoted TNF-α, IL-1ß, IL-6 and IL-18 levels, compared with control negative group. However, over-expression of Circ 0001723 reduced TNF-α, IL-1ß, IL-6 and IL-18 levels in vitro model. Down-regulation of Circ 0001723 suppressed HIF-1α protein expressions and induced NLRP3 and Caspase-1 protein expressions in vitro model by up-regulation of miR-380-3p. Next, inactivation of HIF-1α reduced the pro-inflammation effects of Circ 0001723 in vitro model. Then, si-NLRP3 also inhibited the pro-inflammation effects of Circ 0001723 in vitro model via promotion of autophagy.CONCLUSIONS:
We concluded that HIF-1α reduced inflammation in spinal cord injury via miR-380-3p/ NLRP3 by Circ 0001723.Palavras-chave
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Base de dados:
LILACS
Assunto principal:
Traumatismos da Medula Espinal
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MicroRNAs
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Subunidade alfa do Fator 1 Induzível por Hipóxia
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Proteína 3 que Contém Domínio de Pirina da Família NLR
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RNA Circular
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Inflamação
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article