Transmisión vertical del virus de la rabia cría-madre, fenómeno que podría mantener al virus en especies reservorios de vida silvestre / Vertical transmission of the rabies virus from calf to mother, a phenomenon that could maintain the virus in reservoir species of wildlife
Gac. méd. Méx
; 155(3): 249-253, may.-jun. 2019. tab, graf
Article
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| ID: biblio-1286500
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RESUMEN
Resumen Introducción:
La prueba biológica establecida por la Organización Mundial de la Salud para aislar y amplificar el virus de la rabia consiste en inocular vía intracraneal ratones lactantes para detectar signos de rabia en un periodo de 21 días.Objetivo:
Constatar el contagio viral en las madres de ratones lactantes inoculados con virus de la rabia.Método:
Veintisiete aislados mexicanos de virus de la rabia se inocularon vía intracraneal en ratones lactantes, los cuales fueron observados por 21 días y sus madres, por 60 días. El diagnóstico se llevó a cabo mediante inmunofluorescencia en cerebro. El virus se caracterizó por secuenciación y anticuerpos monoclonales.Resultados:
Todas las camadas presentaron rabia entre siete y 15 días posinoculación (p. i.); tres de las 27 hembras (11 %), a los días 33, 37 y 39 p. i. de sus crías. La caracterización viral mostró que las madres se infectaron con la misma variante de sus crías, dos procedían de murciélago hematófago y una de perro. Las camadas que trasmitieron rabia a sus madres fueron nueve individuos.Conclusiones:
En la naturaleza, el virus de la rabia podría preservarse mediante la transmisión de los neonatos (más susceptibles de contraer y amplificar el virus) a sus madres.ABSTRACT
Abstract Introduction:
The biological test established by the World Health Organization to isolate and amplify the rabies virus consists in inoculating lactating mice by intracranial route and detecting rabies signs for 21 days.Objective:
To verify viral transmission in mothers of rabies virus-inoculated lactating mice.Method:
Twenty-seven Mexican rabies virus isolates were inoculated by intracranial route in lactating mice, which were observed for 21 days. The mothers were observed for 60 days. The diagnosis was established by immunofluorescence in brain tissue. The virus was characterized by sequencing and with monoclonal antibodies.Results:
All litters showed rabies at between 7 and 15 days post-inoculation (p. i.). Three of the 27 females (11 %) had developed rabies at days 33, 37 and 39 p. i. of their litters. Viral characterization showed that the mothers were infected with the same variant of their offspring, two of them stemming from hematophagous bat and one from dog. The liters that transmitted rabies to their mothers were nine individuals.Conclusions:
In nature, the rabies virus could be preserved by transmission from neonates (more susceptible to contracting and amplifying the rabies virus) to their mothers.Palavras-chave
Texto completo:
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Base de dados:
LILACS
Assunto principal:
Complicações Infecciosas na Gravidez
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Raiva
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Vírus da Raiva
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Transmissão Vertical de Doenças Infecciosas
Idioma:
En
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Es
Ano de publicação:
2019
Tipo de documento:
Article