Your browser doesn't support javascript.
loading
Acute exercise inhibits gastric emptying of liquids in rats: influence of the NO-cGMP pathway
Cavalcante, A K M; Siqueira, R C L; Feitosa Júnior, V N; de Andrade, C R; Santos, A A; Silva, M T B.
Afiliação
  • Cavalcante, A K M; Centro Universitário UNINTA. Programa de Pós-Graduação em Ciências Biológicas/Biotecnologia. Sobral. BR
  • Siqueira, R C L; Universidade Federal do Ceará. Faculdade de Medicina. Departmento de Fisiologia e Farmacologia. Fortaleza. BR
  • Feitosa Júnior, V N; Universidade Federal do Ceará. Faculdade de Medicina. Departmento de Fisiologia e Farmacologia. Fortaleza. BR
  • de Andrade, C R; Centro Universitário Christus (UniChristus). Laboratorio de Pesquisa Translacional. Fortaleza. BR
  • Santos, A A; Universidade Federal do Ceará. Programa de Pós-Graduação em Ciências Médicas. Fortaleza. BR
  • Silva, M T B; Universidade Federal do Piauí. Departmento de Educação Física. Teresina. BR
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;51(11): e7541, 2018. tab, graf
Article em En | LILACS | ID: biblio-951721
Biblioteca responsável: BR1.1
ABSTRACT
We previously found that acute exercise inhibited the gastric emptying of liquid in awake rats by causing an acid-base imbalance. In the present study, we investigated the involvement of the nitric oxide-cyclic guanosine monophosphate (NO-cGMP) pathway, vasoactive intestinal peptide (VIP), and corticotropin-releasing factor (CRF) peptide in this phenomenon. Male rats were divided into exercise or sedentary group and were subjected to a 15-min swim session against a load (2.5 or 5% b.w.). The rate of gastric emptying was evaluated after 5, 10, or 20 min postprandially. Separate groups of rats were treated with vehicle (0.9% NaCl, 0.1 mL/100 g, ip) or one of the following agents atropine (1.0 mg/kg, ip), the NO non-selective inhibitor Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME; 10.0 mg/kg, ip), or the selective cGMP inhibitor 1H-(1,2,4)oxadiazole[4,3-a]quinoxalin-1-one (ODQ; 5.0 mg/kg, ip), the i-NOS non-specific inhibitor (aminoguanidine; 10.0 mg/kg, ip), the corticotropin-releasing factor receptor antagonist (astressin; 100 µg/kg, ip), or the vasoactive intestinal peptide (VIP) receptor antagonist Lys1, Pro2,5, Arg3,4, Tyr6 (100 µg/kg, ip). Compared to sedentary rats, both the 2.5 and 5% exercise groups exhibited higher (P<0.05) values of blood lactate and fractional gastric dye recovery. Corticosterone and NO levels increased (P<0.05) in the 5% exercised rats. Pretreatment with astressin, VIP antagonist, atropine, L-NAME, and ODQ prevented the increase in gastric retention caused by exercise in rats. Acute exercise increased gastric retention, a phenomenon that appears to be mediated by the NO-cGMP pathway, CRF, and VIP receptors.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: LILACS Assunto principal: Hormônio Liberador da Corticotropina / Guanosina Monofosfato / Esvaziamento Gástrico / Óxido Nítrico Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: LILACS Assunto principal: Hormônio Liberador da Corticotropina / Guanosina Monofosfato / Esvaziamento Gástrico / Óxido Nítrico Idioma: En Ano de publicação: 2018 Tipo de documento: Article