Estudio de la acción del suero antiloxoscélico sobre los efectos hemolíticos y ulcero-necróticos cutáneos producidos por el veneno de la araña loxosceles laeta / Study of antiloxosceles serum action on hemolytic and ulcero-necrotic cutaneous effects of loxosceles laeta venom

ABSTRACT

The action of an anti-loxosceles serum on in vitro human red blood cell hemolysis and on the development of ulcer-necrotic lesions in rabbit skin, induced by loxosceles laeta venom, was studied. An 81ñ 3 per cent hemolysis was obtained after 72 h incubation of a 2.5 per cent Rh+ red blood cell solution with the equivalent of one Loxosceles laeta venom gland. This parameter was not modified adding anti-loxosceles serum before, along with or after the venom (79.7 ñ 0.8, 77.3 ñ 2.1 and 80.7 ñ 0.7 per cent respectively). After the intradermic injection of a minimal necrotizing venom dose in rabbits, a skin necrotic lesion appeared. This lesion did not appear if anti-loxosceles serum was injected together with the venom; if the serum was injected one hour after the venom, the resulting skin lesion was inflammatory but not necrotic. It is conclude that anti-loxoscles serum does not inhibit loxosceles venom induced hemolysis and causes a time dependent inhibition on skin necrotic lesions