Molecular characterization of Listeria monocytogenes isolated from foods
Rev. microbiol
; 30(4): 356-61, out.-dez. 1999. tab
Article
em En
| LILACS
| ID: lil-286791
Biblioteca responsável:
BR32.1
ABSTRACT
A total of 30 strains of "Listeria monocytogenes" isolated from different foods (16 of different kinds of sausage, 14 cheese) purchased at groceries of São Paulo City were ribotyped and analysed for the presence and expression of hemolysin gene and production of phosphatidylinositol-specific phospholipase C-PI-PLC enzyme. The "L. monocytogenes" strains were differentiated into six ribotype classes. A total of 13 (43.3(per cent)) from these strains belong to the same ribotype (ribotype I), and was coincident to the ribotype of the standard "L. monocytogenes" prototype strain (ATCC-15313). The hemolytic activity was observed in 29 (96.7(per cent)) strains when incubated at 37ºC, but not at 4ºC. The direct colony hibridizatioon method for hemolysin gene detection showed a positive reaction with other "Listeria" spp. The PI-PLC was produced by (90(per cent)) of the strains analysed. There was no correlation between the six identified ribotypes and the virulence facors (hemolysin and PI-PLC) studied
Texto completo:
1
Base de dados:
LILACS
Assunto principal:
Infecções Bacterianas
/
Análise de Alimentos
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Listeria monocytogenes
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Produtos da Carne
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article