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Molecular typing of dengue virus type 2 in Brazil
Miagostovich, Marize P; Sequeira, Patrícia C; Santos, Flávia B. dos; Maia, Amanda; Nogueira, Rita Maria R; Schatzmayr, Hermann G; Harris, Eva; Riley, Lee W.
Afiliação
  • Miagostovich, Marize P; Instituto Oswaldo Cruz. Departmento de Virologia. Laboratório de Flavivirus. Rio de Janeiro. BR
  • Sequeira, Patrícia C; Instituto Oswaldo Cruz. Departmento de Virologia. Laboratório de Flavivirus. Rio de Janeiro. BR
  • Santos, Flávia B. dos; Instituto Oswaldo Cruz. Departmento de Virologia. Laboratório de Flavivirus. Rio de Janeiro. BR
  • Maia, Amanda; Instituto Oswaldo Cruz. Departmento de Virologia. Laboratório de Flavivirus. Rio de Janeiro. BR
  • Nogueira, Rita Maria R; Instituto Oswaldo Cruz. Departmento de Virologia. Laboratório de Flavivirus. Rio de Janeiro. BR
  • Schatzmayr, Hermann G; Instituto Oswaldo Cruz. Departmento de Virologia. Laboratório de Flavivirus. Rio de Janeiro. BR
  • Harris, Eva; University of California. School of Public Health. Division of Infectious Diseases. Berkeley. US
  • Riley, Lee W; University of California. School of Public Health. Division of Infectious Diseases. Berkeley. US
Rev. Inst. Med. Trop. Säo Paulo ; Rev. Inst. Med. Trop. Säo Paulo;45(1): 17-21, Jan.- Feb. 2003. ilus, tab
Article em En | LILACS | ID: lil-330507
Biblioteca responsável: BR1.1
RESUMO
Strain typing is a critical tool for molecular epidemiological analysis and can provide important information about the spread of dengue viruses. Here, we performed a molecular characterization of DEN-2 viruses isolated in Brazil during 1990-2000 from geographically and temporally distinct areas in order to investigate the genetic distribution of this serotype circulating in the country. Restriction site-specific polymerase chain reaction (RSS)-PCR presented the same pattern for all 52 Brazilian samples, showing the circulation of just one DEN-2 variant. Phylogenetic analysis using progressive pairwise alignments from 240-nucleotide sequences of the E/NS1 junction in 15 isolates showed that they belong to genotype III (Jamaica genotype)
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Texto completo: 1 Base de dados: LILACS Assunto principal: Vírus da Dengue Idioma: En Ano de publicação: 2003 Tipo de documento: Article / Project document
Texto completo
Imprimir
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Buscar no Google
Texto completo: 1 Base de dados: LILACS Assunto principal: Vírus da Dengue Idioma: En Ano de publicação: 2003 Tipo de documento: Article / Project document