Diterpenoids from Azorella compacta (Umbelliferae) active on Trypanosoma cruzi
Mem. Inst. Oswaldo Cruz
; 98(3): 413-418, Apr. 2003. ilus, tab
Article
em En
| LILACS
| ID: lil-340125
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BR1.1
ABSTRACT
The anti-Trypanosoma cruzi activity of natural products isolated from Azorella compacta was evaluated, with particular emphasis on their effect against intracellular amastigotes. Five diterpenoids from A. compacta derived from mulinane and azorellane were isolated and identified. Only two products, named azorellanol (Y-2) and mulin-11,3-dien-20-oic acid (Y-5), showed trypanocidal activity against all stages of T. cruzi including intracellular amastigotes. At 10 æM, these compounds displayed a strong lytic activity. It ranged from 88.4 ± 0.6 to 99.0 ± 1 percent for all strains and stages evaluate, with an IC50 /18 h values of 20-84 æM and 41-87 æM, respectively. The development of intracellular amastigotes was also inhibited by nearly 60 percent at 25 æM. The trypanocidal molecules Y-2 and Y-5 did show different degrees of cytotoxicity depending on the cell line tested, with an IC50 /24 h ranging from 33.2 to 161.2 æM. We evaluated the effect of diterpenoids against intracellular T. cruzi forms by immunofluorescent identification of a specific membrane molecular marker (Ssp-4 antigen) of the T. cruzi amastigote forms. The accuracy and reproducibility of the measurements were found to be outstanding when examined by confocal microscopy
Texto completo:
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Base de dados:
LILACS
Assunto principal:
Tripanossomicidas
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Trypanosoma cruzi
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Extratos Vegetais
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Bryopsida
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Diterpenos
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article
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Project document