Hepatic stereology of schistosomiasis mansoni infected-mice fed a high-fat diet
Mem. Inst. Oswaldo Cruz
; 101(supl.1): 253-260, Oct. 2006. ilus, tab, graf
Article
em En
| LILACS
| ID: lil-441255
Biblioteca responsável:
BR1.1
ABSTRACT
High-fat diets induce weight gain and fatty liver in wild-type mice. Schistosomiasis mansoni infection also promotes hepatic injury. This study was designed to quantify hepatic alterations in schistosomiasis mansoni-infected mice fed a high fat-rich chow compared to mice fed a standard rodent chow, using stereology. Female SW mice fed each either high-fat diet (29 percent lipids) or standard chow (12 percent lipids) over 8 months, and then were infected with Schistosoma mansoni cercariae. Four experimental groups were studied infected mice fed a high-fat diet (IHFC) or standard chow (ISC), uninfected mice fed a high-fat diet (HFC) or standard chow (SC). Mice were sacrificed during early infection (9 weeks from exposure). The following hepatic biometry and the stereology parameters were determined volume density (hepatocytes [h], sinusoids [s], steatosis [st] and hepatic fibrosis [hf]); numerical density (hepatocyte nuclei - Nv[h]); absolute number of total hepatocyte N[h], normal hepatocyte N[nh], and binucleated hepatocyte N[bh], percentage of normal hepatocyte P[nh] and binucleated hepatocyte P[bh]. IHFC and HFC groups exhibited TC, HDL-C, LDL-C, and body mass significantly greater (p < 0.05) than control group. No significant differences were found regards liver volume (p = 0.07). Significant differences were observed regards P[nh] (p = 0.0045), P[bh] (p = 0.0045), Nv[h] (p = 0.0006), N[h] (p = 0.0125), N[bh] (p = 0.0164) and N[nh] (p = 0.0078). IHFC mice group presented 29 percent of binucleated hepatocytes compared to HFC group (19 percent), ISC group (17 percent) and SC (6 percent). Volume density was significantly different between groups Vv[h] (p = 0.0052), Vv[s] (p = 0.0025), Vv[st] (p = 0.0004), and Vv[hf] (p = 0.0007). In conclusion, schistosomiasis mansoni infection with concurrent high-fat diet promotes intensive quantitative changes in hepatic structure, contributing to an increasing on hepatic regeneration.
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Base de dados:
LILACS
Assunto principal:
Esquistossomose mansoni
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Gorduras na Dieta
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Fígado
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Obesidade
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article