Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight microsatellites
Genet. mol. res. (Online)
; 7(1): 29-32, Jan. 2008. tab
Article
em En
| LILACS
| ID: lil-553767
Biblioteca responsável:
BR26.1
ABSTRACT
The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtained demonstrate high heterozygosity (average of 0.69) in a Lower Amazon population. The multiplex system described can thus be considered a fast, efficient and inexpensive method for the investigation of genetic variability in Arapaima populations.
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Texto completo:
1
Base de dados:
LILACS
Assunto principal:
Variação Genética
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Reação em Cadeia da Polimerase
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Repetições de Microssatélites
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Peixes
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article