Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;47(1): 50-59, 01/2014. tab, graf
Article
em En
| LILACS
| ID: lil-697673
Biblioteca responsável:
BR1.1
ABSTRACT
Cardiovascular disease is one of the leading causes of death worldwide, and evidence indicates a correlation between the inflammatory process and cardiac dysfunction. Selective inhibitors of cyclooxygenase-2 (COX-2) enzyme are not recommended for long-term use because of potentially severe side effects to the heart. Considering this and the frequent prescribing of commercial celecoxib, the present study analyzed cellular and molecular effects of 1 and 10 µM celecoxib in a cell culture model. After a 24-h incubation, celecoxib reduced cell viability in a dose-dependent manner as also demonstrated in MTT assays. Furthermore, reverse transcription-polymerase chain reaction analysis showed that the drug modulated the expression level of genes related to death pathways, and Western blot analyses demonstrated a modulatory effect of the drug on COX-2 protein levels in cardiac cells. In addition, the results demonstrated a downregulation of prostaglandin E2 production by the cardiac cells incubated with celecoxib, in a dose-specific manner. These results are consistent with the decrease in cell viability and the presence of necrotic processes shown by Fourier transform infrared analysis, suggesting a direct correlation of prostanoids in cellular homeostasis and survival.
Palavras-chave
Texto completo:
1
Base de dados:
LILACS
Assunto principal:
Pirazóis
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Sulfonamidas
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Sobrevivência Celular
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Regulação da Expressão Gênica
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Mioblastos Cardíacos
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Proliferação de Células
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article
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Project document