Bioinformatics based structural characterization of glucose dehydrogenase (gdh) gene and growth promoting activity of Leclercia sp. QAU-66
Braz. j. microbiol
; 45(2): 603-611, Apr.-June 2014. ilus, tab
Article
em En
| LILACS
| ID: lil-723124
Biblioteca responsável:
BR1.1
ABSTRACT
Glucose dehydrogenase (GDH; EC 1.1. 5.2) is the member of quinoproteins group that use the redox cofactor pyrroloquinoline quinoine, calcium ions and glucose as substrate for its activity. In present study, Leclercia sp. QAU-66, isolated from rhizosphere of Vigna mungo, was characterized for phosphate solubilization and the role of GDH in plant growth promotion of Phaseolus vulgaris. The strain QAU-66 had ability to solubilize phosphorus and significantly (p < 0.05) promoted the shoot and root lengths of Phaseolus vulgaris. The structural determination of GDH protein was carried out using bioinformatics tools like Pfam, InterProScan, I-TASSER and COFACTOR. These tools predicted the structural based functional homology of pyrroloquinoline quinone domains in GDH. GDH of Leclercia sp. QAU-66 is one of the main factor that involved in plant growth promotion and provides a solid background for further research in plant growth promoting activities.
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Texto completo:
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Base de dados:
LILACS
Assunto principal:
Phaseolus
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Glucose 1-Desidrogenase
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Enterobacteriaceae
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Fatores de Crescimento Neural
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article