Crystallization and preliminary X-ray analysis of the Rhodobacter capsulatus magnesium chelatase BchI subunit.
Acta Crystallogr D Biol Crystallogr
; 55(Pt 3): 689-90, 1999 Mar.
Article
em En
| MEDLINE
| ID: mdl-10089453
ABSTRACT
The Rhodobacter capsulatus BchI protein is one of three subunits of Mg chelatase, the enzyme which catalyzes the first committed step of chlorophyll and bacteriochlorophyll biosynthesis. The BchI protein was produced with an inducible T7 RNA polymerase expression system in Escherichia coli. The protein was purified from the soluble cell-extract fraction and crystallized from polyethylene glycol solution. The crystals diffract to a minimum Bragg spacing of 2.1 A. The space group is P63 with unit-cell dimensions a = b = 90.6, c = 84.1 A.
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Base de dados:
MEDLINE
Assunto principal:
Rhodobacter capsulatus
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Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article