Expression of platelet-activating factor receptor transcript-1 but not transcript-2 by human bone marrow cells.
Stem Cells
; 17(2): 121-4, 1999.
Article
em En
| MEDLINE
| ID: mdl-10195573
ABSTRACT
The presence of platelet-activating factor receptor (PAF-R) transcripts 1 and 2 was investigated in human bone marrow cells by a reverse transcriptase polymerase chain reaction (RT-PCR) procedure which detected their simultaneous presence. RT-PCR experiments reveal PAF-R transcript 1 (but not 2) in freshly isolated mononuclear marrow cells, CD34+ hematopoietic stem/progenitor cells and cultured marrow stromal cells. For these experiments, the 5637 human bladder carcinoma cell line is used as a positive control for the presence of PAF-R transcripts 1 and 2. Flow cytometry experiments confirm the presence of PAF-R on marrow stromal cells and CD34+ stem/progenitor cells. In conclusion, the expression of PAF-R transcript 1, which mainly exists in circulating leukocytes, is also found in CD34+ stem/progenitor cells and cells of the marrow microenvironment, strengthening the potential role of PAF during marrow hematopoiesis.
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Base de dados:
MEDLINE
Assunto principal:
Células da Medula Óssea
/
Glicoproteínas da Membrana de Plaquetas
/
Expressão Gênica
/
Receptores de Superfície Celular
/
Receptores Acoplados a Proteínas G
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article