Multiple-pulse-mediated electrofusion of intact erythrocyte onto human term placental amnion.

The creation of surface modified human term placental amnion by electrofusing human cells onto its surface has been thought of. A multiple-pulse electrofusion protocol with 10 square pulses of 10-micros pulse length, and electric field of 0.2 kV cm(-1), can make erythrocyte-amnion tissue electrofusion possible. The protocol devised merge the cell-tissue-adherence steps with fusogenic pulse. The finding opens up a new avenue of cell electrofusion onto human tissue with minimal procedural complexities.