Proteins from nuclear extracts of two lepidopteran cell lines recognize the ends of TTAA-specific transposons piggyBac and tagalong.
Insect Mol Biol
; 8(2): 223-30, 1999 May.
Article
em En
| MEDLINE
| ID: mdl-10380106
ABSTRACT
The transposons piggyBac and tagalong are Lepidopteran transposons that exhibit extreme site-specificity for the tetranucleotide TTAA upon insertion and excise in a characteristic precise fashion, regenerating a single TTAA target site. The precise excision of both piggyBac and tagalong can occur in the absence of factors encoded by either transposon, possibly through the recruitment of host proteins and/or cross-mobilizing transposons. In this report, we utilize mobility shift assays and exonuclease III protection analyses to identify DNA binding activities from IPLB-SF21AE and TN-368 cells that are specific for piggyBac and tagalong-terminal repeats.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Nucleares
/
Elementos de DNA Transponíveis
/
Proteínas de Insetos
/
Proteínas de Ligação a DNA
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article