Direct interaction in T-cells between thetaPKC and the tyrosine kinase p59fyn.
J Biol Chem
; 274(27): 19003-10, 1999 Jul 02.
Article
em En
| MEDLINE
| ID: mdl-10383400
ABSTRACT
The protein kinase C (PKC) family has been clearly implicated in T-cell activation as have several nonreceptor protein-tyrosine kinases associated with the T-cell receptor, including p59fyn. This report demonstrates that thetaPKC and p59fyn specifically interact in vitro, in the yeast two-hybrid system, and in T-cells. Further indications of direct interaction are that p59fyn potentiates thetaPKC catalytic activity and that thetaPKC is a substrate for tyrosine phosphorylation by p59fyn. This interaction may account for the localization of thetaPKC following T-cell activation, pharmacological disruption of which results in specific cell-signaling defects. The demonstration of a physical interaction between a PKC and a protein-tyrosine kinase expands the class of PKC-anchoring proteins (receptors for activated C kinases (RACKs)) and demonstrates a direct connection between these two major T-cell-signaling pathways.
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Base de dados:
MEDLINE
Assunto principal:
Proteína Quinase C
/
Proteínas Tirosina Quinases
/
Linfócitos T
/
Proteínas Proto-Oncogênicas
/
Isoenzimas
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article