[Effect of oxidized low density lipoproteins on the structure of platelet membrane. Use of electron paramagnetic resonance]. / Vliianie okislennykh lipoproteinov nizkoi plotnosti na strukturu plazmaticheskoi membrany trombotsitov. Issledovanie metodom élektronnogo paramagnitnogo rezonansa.
Biofizika
; 44(2): 272-80, 1999.
Article
em Ru
| MEDLINE
| ID: mdl-10418677
ABSTRACT
The effect of low-density lipoproteins on the structure of platelet plasma membrane was studied by electron paramagnetic resonance spectroscopy. Low-density lipoproteins were incubated with platelet rich plasma at a volume ratio 11. Plasma incubated with buffer served as a control. After incubation, the fluidity of platelet plasma membrane was determined by electron spin resonance probes 5-doxylstearate and 16-doxylstearate, which were immobilized in membranes of cells subjected to triple precipitation. Significant differences in the order parameter S, which characterizes the spectrum of the 5-doxylstearate probe, for samples incubated with the buffer and oxidized low-density lipoproteins were found. The dependence of the parameter on incubation time and the extend of oxidation of low-density lipoproteins were obtained. No significant differences in rotational correlation time of 16-doxylstearate between platelets incubated with and without oxidized low-density lipoproteins was observed within the limits of experimental error; however, the changes in the half-width of the low-field component may be considered reliable. The interaction of oxidized low-density lipoproteins with platelets leads to an increase in plasma membrane fluidity, thereby mediating the activating action on platelets.
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Base de dados:
MEDLINE
Assunto principal:
Plaquetas
/
Lipoproteínas LDL
Idioma:
Ru
Ano de publicação:
1999
Tipo de documento:
Article