Virus-specific capping of tobacco mosaic virus RNA: methylation of GTP prior to formation of covalent complex p126-m7GMP.
FEBS Lett
; 455(1-2): 45-8, 1999 Jul 16.
Article
em En
| MEDLINE
| ID: mdl-10428469
ABSTRACT
In capping cellular mRNAs, a covalent GMP-enzyme intermediate leads to formation of G(5')ppp(5')N at the 5' end of the RNA, which is modified by methylation catalyzed by guanine-7-methyltransferase. Here we show that isolated membranes from tobacco mosaic virus (TMV)-infected plant or insect cells expressing TMV replicase protein p126, synthesized m7GTP using S-adenosylmethionine (AdoMet) as the methyl donor, and catalyzed the formation of a covalent guanylate-p126 complex in the presence of AdoMet. The methyl group from AdoMet was incorporated into p126, suggesting that the complex consisted of m7GMP-p126. Thus, TMV and alphaviruses, despite their evolutionary distance, share the same virus-specific capping mechanism.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Vírus do Mosaico do Tabaco
/
Capuzes de RNA
/
RNA Viral
/
Guanosina Monofosfato
/
Guanosina Trifosfato
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article