Annexin V binds to the actin-based cytoskeleton at the plasma membrane of activated platelets.
Exp Cell Res
; 251(1): 185-93, 1999 Aug 25.
Article
em En
| MEDLINE
| ID: mdl-10438584
ABSTRACT
Immunocytochemical studies demonstrate that annexin V relocates to the plasma membranes of intact stimulated blood platelets. Anti-annexin V antibodies label the cytoplasmic side of the substrate-adherent plasma membrane of mechanically unroofed, glass-activated platelets and colocalize with actin. In addition, crosslinking experiments using detergent-solubilized membranes of activated platelets have identified an 85-kDa complex containing annexin V. The 85-kDa complex is also recognized by antibodies against actin, suggesting that annexin V interacts with actin. In addition, annexin V was found to associate with filamentous actin in the presence of millimolar Ca(2+). Annexin V was also shown by immunofluorescence microscopy to be associated with platelet cytoskeletons, colocalizing with actin in the presence of micromolar Ca(2+). These findings provide the first evidence for annexin V binding to the plasma membrane and to the actin-based cytoskeleton in activated platelets and indicate that annexin V may function in both cytoskeletal and membrane domains.
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Base de dados:
MEDLINE
Assunto principal:
Plaquetas
/
Citoesqueleto
/
Ativação Plaquetária
/
Membrana Celular
/
Actinas
/
Anexina A5
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article