A Drosophila Axin homolog, Daxin, inhibits Wnt signaling.
Development
; 126(18): 4165-73, 1999 Sep.
Article
em En
| MEDLINE
| ID: mdl-10457025
ABSTRACT
The vertebrate Axin protein, the product of the mouse fused gene, binds to beta-catenin to inhibit Wnt signaling. We have identified a homolog of Axin in Drosophila, Daxin. Using double-stranded RNA interference, we generated loss-of-function phenotypes that are similar to overexpression of the Drosophila Wnt gene wingless (wg). Overexpression of Daxin produces phenotypes similar to loss of wg. In addition, we show that Daxin overexpression can modify phenotypes elicited by wg and another Drosophila Wnt gene, DWnt-2. Using immunoprecipitation of endogenous Daxin protein from embryos we show that Daxin interacts with Armadillo and Zeste-white 3. The loss-of-function and overexpression phenotypes show that Daxin, like its mammalian counterpart, acts as a negative regulator of wg/Wnt signaling.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
/
Transdução de Sinais
/
Proteínas de Transporte
/
Transativadores
/
Proteínas Proto-Oncogênicas
/
Proteínas de Drosophila
/
Quinase 3 da Glicogênio Sintase
/
Proteínas Adaptadoras de Transdução de Sinal
/
Drosophila melanogaster
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article