Expression of a functional antibody fragment in the gut of Rhodnius prolixus via transgenic bacterial symbiont Rhodococcus rhodnii.
Med Vet Entomol
; 13(2): 115-9, 1999 May.
Article
em En
| MEDLINE
| ID: mdl-10484156
ABSTRACT
Expression within insects of foreign antiparasitic gene products via microbial symbionts could be used to prevent transmission of vector-borne pathogens to vertebrate hosts. Genetically transformed symbiotic bacteria Rhodococcus rhodnii expressed functional antibody fragments (rDB3 encoding murine V(H)/K which binds progesterone) that were exported into the gut lumen of the triatomine bug Rhodnius prolixus (Hemiptera Reduviidae), a vector of Chagas disease. Transgenic symbionts were maintained in successive nymphal instars and adults of Rhodnius prolixus despite competition with native untransformed Rhodococcus rhodnii. This is the first description of a functional mammalian antibody fragment expressed in an insect. Our system is a model for constructing paratransgenic insects (insects carrying transformed symbionts) with compromised ability to transmit pathogens.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Rhodnius
/
Rhodococcus
/
Região Variável de Imunoglobulina
/
Fragmentos de Imunoglobulinas
/
Cadeias Pesadas de Imunoglobulinas
/
Vetores Genéticos
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article