Localization of dopamine D1 and D2 receptor mRNAs in the rat systemic and pulmonary vasculatures.
Mol Cells
; 9(4): 417-21, 1999 Aug 31.
Article
em En
| MEDLINE
| ID: mdl-10515606
ABSTRACT
The present study was designed to evaluate the expression of dopamine D1 and D2 receptor mRNAs in systemic and pulmonary vasculatures. Using specific antisense riboprobes for dopamine D1 and D2 receptor cDNAs, in situ hybridization histochemistry was performed in the aorta, common carotid artery, vertebral artery, pulmonary artery, and superior vena cava of the adult male Sprague Dawley rat. In the case of the aorta, common carotid artery, and vertebral artery, dopamine D1 receptor mRNAs localized mainly in the smooth muscle cells of the tunica media. However, the signals of dopamine D2 receptor mRNAs were found in the endothelium and subendothelial layer of tunica intima, and interstitial cells of tunica adventitia. In the case of the pulmonary artery, signals of dopamine D1 receptor mRNAs were detected within the tunica intima, media, and adventitia. Expression of D2 receptor mRNAs was detected in the walls of small blood vessels within the tunica adventitia of the pulmonary artery. There were no detectable signals of dopamine D1 and D2 receptor mRNAs in the vein. The uneven distribution of dopamine D1 and D2 receptor mRNAs in the rat systemic vasculatures and pulmonary artery suggests that dopamine differentially regulates the vasodilation of the systemic and pulmonary arteries through the differential stimulation of dopamine D1 and D2 receptor.
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Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Endotélio Vascular
/
Circulação Pulmonar
/
Receptores de Dopamina D2
/
Receptores de Dopamina D1
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article