Sphingosine 1-phosphate induces arachidonic acid mobilization in A549 human lung adenocarcinoma cells.
Biochim Biophys Acta
; 1483(1): 154-60, 2000 Jan 03.
Article
em En
| MEDLINE
| ID: mdl-10601704
ABSTRACT
In the present paper, the effect of sphingosine 1-phosphate (Sph-1-P) on arachidonic acid mobilization in A549 human lung adenocarcinoma cells was investigated. Sph-1-P provoked a rapid and relevant release of arachidonic acid which was similar to that elicited by bradykinin, well-known pro-inflammatory agonist. The Sph-1-P-induced release of arachidonic acid involved Ca(2+)-independent phospholipase A(2) (iPLA2) activity, as suggested by the dose-dependent inhibition exerted by the rather specific inhibitor bromoenol lactone. The Sph-1-P-induced release of arachidonic acid was pertussis toxin-sensitive, pointing at a receptor-mediated mechanism, which involves heterotrimeric Gi proteins. The action of Sph-1-P was totally dependent on protein kinase C (PKC) catalytic activity and seemed to involve agonist-stimulated phospholipase D (PLD) activity. This study represents the first evidence for Sph-1-P-induced release of arachidonic acid which occurs through a specific signaling pathway involving Gi protein-coupled receptor(s), PKC, PLD and iPLA2 activities.
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Base de dados:
MEDLINE
Assunto principal:
Esfingosina
/
Lisofosfolipídeos
/
Ácido Araquidônico
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article