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Determination of rotenone residues in raw honey by solid-phase extraction and high-performance liquid chromatography.
Jiménez, J J; Bernal, J L; del Nozal, M J; Novo, M; Higes, M; Llorente, J.
Afiliação
  • Jiménez JJ; Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid, Spain.
J Chromatogr A ; 871(1-2): 67-73, 2000 Feb 25.
Article em En | MEDLINE | ID: mdl-10735287
A method for determining residues of the insecticide rotenone in raw-honey by high-performance liquid chromatography (HPLC) is described. To extract the residues, organic solvents such as ethyl acetate, n-hexane/dichloromethane and solid-phase extraction with octadecylsilane cartridges or Florisil packed columns were tested. Determination was carried out by reversed-phase HPLC using acetonitrile-buffer phosphate (pH 7) (60:40, v/v) as mobile phase and detection at 210 nm. Although the data showed that the two extraction methods were able to isolate the pesticide residues, the extraction on octadecylsilane cartridges was preferred due to its simplicity and higher recovery. Recoveries depended strongly on the fortification level for the two extraction procedures. Practical determination limits of 0.015 mg/kg were obtained. In the analysis of honeys, from beehives treated with rotenone at therapeutical doses for 1 month, residual amounts below 0.2 mg/kg were found.
Assuntos
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Base de dados: MEDLINE Assunto principal: Rotenona / Resíduos de Praguicidas / Cromatografia Líquida de Alta Pressão / Mel / Inseticidas Idioma: En Ano de publicação: 2000 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Rotenona / Resíduos de Praguicidas / Cromatografia Líquida de Alta Pressão / Mel / Inseticidas Idioma: En Ano de publicação: 2000 Tipo de documento: Article