Cloning and characterization of a gene complementing the mutation of an ethanol-sensitive mutant of sake yeast.
Biosci Biotechnol Biochem
; 64(2): 229-36, 2000 Feb.
Article
em En
| MEDLINE
| ID: mdl-10737174
ABSTRACT
Ethanol-sensitive mutants (esl to es10) were isolated from sake yeast, Saccharomyces cerevisiae SY-32. These mutants were unable to grow at 7% ethanol at which the wild type strain SY-32 does grow. The mutants had a variety of fermentation rates and viabilities in the presence of ethanol. The gene ERG6, complementing the ethanol-sensitive mutation of es5, was cloned from an SY-32 gene library. ERG6 encodes S-adenosylmethionine delta 24-sterol-C-methyltransferase (EC 2.1.1.41) in the ergosterol synthetic pathway. Mutant es5 had a reduced ability to synthesize ergosterol. An erg6 disruptant was also ethanol-sensitive. These results suggested that ERG6 plays an important role in the ethanol tolerance of S. cerevisiae.
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Base de dados:
MEDLINE
Assunto principal:
Vinho
/
Leveduras
/
Etanol
/
Teste de Complementação Genética
/
Mutação
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article